This study examined the phenol degradation capabilities and oxidative stress responses of Candida tropicalis SHC-03, demonstrating its metabolic superiority and resilience compared to Saccharomyces cerevisiae BY4742 in a culture medium with phenol as the sole carbon source. Through comparative growth, transcriptomic, and metabolomic analyses under different phenol concentrations, this study revealed C. tropicalis SHC-03’s specialized adaptations for thriving in phenol as the sole carbon source environments. These include a strategic shift from carbohydrate metabolism to enhanced phenol degradation pathways, highlighted by the significant upregulation of genes for Phenol 2-monoxygenase and Catechol 1,2-dioxygenase. Despite phenol levels reaching 1.8 g/L, C. tropicalis exhibits a robust oxidative stress response, efficiently managing ROS through antioxidative pathways and the upregulation of genes for peroxisomal proteins like PEX2 , PEX13 , and PMP34 . Concurrently, there was significant upregulation of genes associated with membrane components and transmembrane transporters, enhancing the cell’s capacity for substance exchange and signal transduction. Especially, when the phenol concentration was 1.6 g/L and 1.8 g/L, the degradation rates of C. tropicalis towards it were 99.47 and 95.91%, respectively. Conversely, S. cerevisiae BY4742 shows limited metabolic response, with pronounced growth inhibition and lack of phenol degradation. Therefore, our study not only sheds light on the molecular mechanisms underpinning phenol tolerance and degradation in C. tropicalis but also positions this yeast as a promising candidate for environmental and industrial processes aimed at mitigating phenol pollution.
Social development requires us to train socialist builders and successors who are all-round developed morally, intellectually, physically, and professionally.Music education is an important component of art education, and moral education teaching is a humanized and moral teaching way.The cultivation of students is an important part of students' moral education in the task of modern teaching.The beauty of art and ideological and moral education can help students form a correct world outlook, outlook on life and values, cultivate students' sentiment, beautify students' behavior, and make students receive subtle education.Through investigation and research, this paper mainly analyzes the importance of moral education in middle school music education, tries to combine music education with moral education, and explore the ways to cultivate students' moral education in middle school music teaching by learning patriotic music works, using traditional music perception, and carrying out teamwork activities.To make a little contribution to the establishment of perfect moral education in the future middle school music classroom, but also for the future to better promote the development of students in the music classroom.
Cell tracking in chimeric models is essential yet challenging, particularly in developmental biology, regenerative medicine, and transplantation studies. Existing methods, such as fluorescent labeling and genetic barcoding, are technically demanding, costly, and often impractical for dynamic, heterogeneous tissues. To address these limitations, we propose a computational framework that leverages sex as a surrogate marker for cell tracking. Our approach uses a machine learning model trained on single-cell transcriptomic data to predict cell sex with high accuracy, enabling clear distinction between donor (male) and recipient (female) cells in sex-mismatched chimeric models. The model identifies specific genes critical for sex prediction and has been validated using public datasets and experimental flow sorting, confirming the biological relevance of the identified cell populations. Applied to skeletal muscle macrophages, our method revealed distinct transcriptional profiles associated with cellular origins. This pipeline offers a robust, cost-effective solution for cell tracking in chimeric models, advancing research in regenerative medicine and immunology by providing precise insights into cellular origins and therapeutic outcomes.
Most of arsenic in the stibnite enters the antimony oxide powder after a traditional volatilization smelting/roasting process and further transfers into an arsenic-alkali residue in the followed antimony smelting process. This transfer of arsenic poses a huge threat to the environment. Decreasing the As transfer, a new process is proposed in this paper including two stages of roasting with pyrite addition for As pre-removal from antimony oxide powder. First, at temperatures higher than 500°C in a nitrogen atmosphere, the As2O3, Sb2O3, and As-Sb solid solution in the antimony oxide powder were transformed to As-S and Sb-S compounds. Then with the oxidizing roasting process, which was carried out at the conditions of an air flow rate of 200 ml/min, roasting time of 90 min, roasting temperature of 550°C and pyrite dosage of 5 wt.%, the As-S compounds were oxidized to As2O3 and volatilized with an As removal rate of 92.71%, while the antimony was transformed to nonvolatile high-valence compounds that remained in roasted residues with Sb loss rate of only 5.64%. Using this treated antimony oxide powder in the traditional antimony smelting process, the formation of arsenic-alkali residue and antimony loss can be decreased to a great extent.
Abstract Background Sediments function as a secondary and significant source of tributyltin (TBT) and triphenyltin (TPT) in aquatic ecosystems and may pose a potential threat on benthic organisms and human health. The subchronic toxicity of sediment-associated TBT or TPT to snails Bellamya aeruginosa at environmentally relevant concentrations was investigated in this study. Multiple endpoints at the biochemical [ethoxyresorufin- O -deethylase (EROD), superoxide dismutase (SOD), catalase (CAT), protein carbonyl content (PCC) and lipid peroxidation (LPO)] and transcriptomic levels were examined. Results TBT or TPT in sediment could induce antioxidant enzymes’ activities and result in oxidative damage in the hepatopancreas of B. aeruginosa after 28-day exposure. A transcriptomic profile of B. aeruginosa exposed to TBT and TPT was reported. CYP genes and EROD activity were sensible and reliable biomarkers for toxicity assessment of TBT or TPT in sediments. Comparative pathway analysis revealed the alteration of steroid hormone biosynthesis and retinol metabolism in B. aeruginosa after 90-day exposure to sediment-associated TBT at the concentration of 2000 ng/g dw, which might affect both reproduction and lipogenesis functions. The ubiquitin proteasome system and immune system might be the toxicity target in B. aeruginosa after exposure to sediment-associated TPT for 90 days. Conclusions The results offered new mechanisms underlying the toxicity of sediment-associated tributyltin and triphenyltin.
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