This dataset contains the digitized treatments in Plazi based on the original journal article Cho, Geonho, Burckhardt, Daniel, Inoue, Hiromitsu, Luo, Xinyu, Lee, Seunghwan (2017): Systematics of the east Palaearctic pear psyllids (Hemiptera: Psylloidea) with particular focus on the Japanese and Korean fauna. Zootaxa 4362 (1): 75-98, DOI: https://doi.org/10.11646/zootaxa.4362.1.4
Abstract Citrus greening (Huanglongbing) disease has serious impacts on citrus production. Field monitoring of the Asian citrus psyllid Diaphorina citri (Hemiptera: Psyllidae), a vector of citrus greening disease, is essential to prevent the invasion and spread of this disease. This study reports a new method for determining the presence of D. citri and traces of contact with host plants by collecting leaves of host plants from the survey area and detecting the environmental DNA (eDNA) derived from D. citri . The results show that with the applied method the presence of D. citri can be determined. As D. citri ‐derived eDNA is contained in DNA solutions of plants with a history of contact with D. citri , we succeeded in detecting not only genes conserved in D. citri mitochondria but also genes of D. citri symbiont organisms consisting of Wolbachia , Wolbachia phage, Candidatus Carsonella sp., and Candidatus Profftella sp. Diaphorina citri ‐derived eDNA could be detected in host plants even after only 10 min of contact with D. citri and could still be detected 6 months after contact. This technology has the potential to trace D. citri from their traces without individual detection and is expected to greatly contribute to the early detection and invasion warning of citrus greening disease in the future.
Abstract Flowers on the ground of orchards can provide substantial resources for wild pollinators of orchard trees. Few studies, however, have examined the relative importance of groundcover flowers to orchard pollination by analyzing pollen on the body surface of pollinators. Oriental persimmon trees bloom within the longer blooming period of white clover, which is occasionally found as a flowering plant on the ground of persimmon orchards in Japan. The present study compared the insect species assemblage collected on persimmon flowers with that on clover. Before persimmon bloomed, Bombus ardens ardens and Apis cerana japonica were the major visitors of clover flowers. Once persimmon bloomed, the former was the most abundant bee that visited persimmon flowers over the flowering period. Apis mellifera was captured only on clover flowers. We found numerous clover pollen grains on the body surface of bumblebees captured on persimmon flowers, but far fewer persimmon pollen grains on bees that visited clover. These findings show that B. ardens ardens utilized the clover flowers under the orchards before persimmon bloomed.
The records of the jumping plant-lice previously published under Carsidara shikokuensis (Miyatake, 1981) from Japan and C. marginalis Walker, 1869 from Korea are critically reviewed. It is concluded that both refer to the same species that is associated with Firmiana simplex (L.) W.Wight (Malvaceae). Carsidara shikokuensis shows no relevant morphological differences to C. limbata (Enderlein, 1926), and the two are synonymised: Carsidara limbata (Enderlein, 1926) = Carsidara shikokuensis (Miyatake, 1981) syn. nov. A record of C. marginalis from Korea is a misidentification of C. limbata. The latter is diagnosed and illustrated and differences to the former are discussed. The immatures of C. limbata are free-living on the leaves of Firmiana simplex and particularly the older instars secrete large amounts of flocculent wax and honeydew. Information on its potential usage as biological control agent of invasive Firmiana simplex in North America is briefly discussed.
Abstract Several European and North American countries have started releasing the Japanese knotweed psyllid Aphalara itadori (Hemiptera: Aphalaridae) to control the Japanese knotweed Reynoutria japonica (Caryophyllales: Polygonaceae) and its relatives, which are among the worst invasive exotic plants. However, establishing populations of the current Kyushu and Hokkaido strains in the field has not been successful, desiring new lineages. Moreover, little is known about the microbiome of the current strains, which potentially impacts properties as biocontrol agents. Hence, this study analyzed the microbiota of an A. itadori strain newly collected on Honshu Island, along with related species of the family Aphalaridae, using amplicon sequencing of 16S rRNA genes. The A. itadori symbionts were further located using fluorescence in situ hybridization. The results demonstrated that the analyzed A. itadori strain has a dual symbiotic system with “ Candidatus Carsonella ruddii” (Gammaproteobacteria: Oceanospirillales) and Sodalis sp. (Gammaproteobacteria: Enterobacterales) harbored in the bacteriome, suggesting their evolutionarily stable mutualistic relationships with A. itadori . The central area of the bacteriome harboring Sodalis appeared to comprise uninucleate bacteriocytes with nuclei larger than those of bacteriocytes for Carsonella , rather than a syncytium with smaller nuclei as previously reported for various psyllid lineages. No known plant pathogens or manipulators of insect reproduction were identified in the analyzed strain, indicating its suitability as a biocontrol agent, posing a minimum risk to the ecosystem. Besides distinct Carsonella lineages, the analysis identified Sodalis independently acquired by Craspedolepta miyatakeai , and an ambiguous Enterobacterales symbiont in Epheloscyta kalopanacis . Only Carsonella was identified in Togepsylla matsumurana .
New junior synonyms are proposed for the Sorbus-feeding Cacopsylla moiwasana (Kuwayama, 1908): Psylla jezoensis Miyatake, 1963, syn. nov. and Psylla midoriae Miyatake, 1963, syn. nov. The species is newly recorded from Korea.
