Objectives To study clinical and coronary angiographic findings in patients with both coronary heart diseases (CHD) and type 2 diabetes mellitus (T2DM). Methods 215 patients with CHD confirmed by coronary angiography were involved in this study. The patients were divided into two groups: 74 CHD patients with T2DM ( mean age 64.7 ±8.2 years, male/female 47/27), and 141 CHD patients without T2DM (mean age 66.2 ± 9.2 years,male/female 100/41 ). The clinical features and the data from selective coronary angiographies were compared between type 2 diabetic and non - diabetic CHD patients. Results Compared to non -diabetic CHD patients, the patients with both CHD and T2DM suffered more from acute myocardial infarction, silent ischemia and severe arrhythmias (P ＜ 0. 01, P ＜ 0. 05 ) ,and had higher serum triglycerides and apo - lipoprotein B, along with increased serum uric acid (P ＜ 0. 01, P ＜0. 05), increased left ventricular end diastolic diameter (P ＜ 0. 01 ), and decreased left ventricular ejection fraction ( P ＜ 0. 001 ). Compared to non - diabetic CHD patients, the patients with both CHD and T2DM suffered more from triple vessel disease ( P ＜ 0. 01 ) ,severe coronary artery stenosis, complete occlusions and diffuse lesions ( P ＜ 0. 001 ). Conclusions Severe clinical manifestation, left ventricular dysfunction,diffuse or complicated lesions of coronary arteries were more common in patients with both CHD and T2DM, it suggests that the type 2 diabetic CHD patients have poor prognosis.
[Objective]This study aimed to investigate the mutations of OmpF from an isolated antibiotic resistant Escherichia coli strain.[Methods]The mutant OmpF(mOmpF)from antibiotic resistant E.coli was amplified by PCR with Pfu DNA polymerase and ligated into the expression vector pET28a.Subsequently,the expression vector pET28-mOmpF was sequenced and analyzed by DNAMAN software and Swiss-Model online.[Result]Sequence analysis revealed that the open reading fragment of mOmpF was 903 bp long,which was mutated dramatically compared to that of the 1 020 bp long model OmpF.The DNA sequence shared only54.5%homology with OmpF.mOmpF was 44.6%identical to that of OmpF.Protein structure predication and analysis through Swiss-Model online suggested that the structure of mOmpF changed dramatically compared to OmpF.[Conclusion]The present study provided basis for further analyzing the relationships between the structure and functions of mOmpF from antibiotic resistant E.coli.
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