Two isostructural 2D supramolecular cocrystal compounds show different dielectric responses and proton conductivities due to the alteration of the carbon-skeleton of piperazine derivatives.
Polyvinylidene fluoride (PVDF) membrane are widely used in water treatment due to its good physical and chemical properties, and the pores of PVDF membranes determine most of the characteristics of PVDF membrane. The hydrophilic porogens play an important role in the preparation of PVDF membranes and the regulation of membrane pores. In this work, three hydrophilic porogens with different quality components were used to prepare PVDF ultrafiltration membranes by the nonsolvent induced phase separation (NIPS) method. Through the Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM), mechanical strength, contact angle and other characterizations, the influence of hydrophilic porogens on the surface free energy, microstructure and performance of PVDF membrane were studied. The results suggest that lithium chloride (LiCl) can inhibit the formation of macropores and improve the mechanical strength of the membrane. Polyvinylpyrrolidone (PVP) can form through-hole through the PVDF membrane. All three hydrophilic porogens can increased the surface free energy of the PVDF membranes, where polyethylene glycol (PEG) had the most impact on the surface free energy.
Introduction: Stress-related gastric mucosal lesions (SGMLs) are the most common complication in critical care patients. Previous studies have demonstrated that herbal pair (HP), Polygonum hydropiper-Coptis chinensis (HP P-C) has the anti-SGML effect. However, the underlying mechanism of HP P-C against SGML remains elusive. This study aimed to elucidate how HP P-C extracts exert their protective effects on SGML by examining the role of gut microbiota and metabolites. Methods: SD rats were pretreated with different doses of HP P-C extracts for 6 days, followed by inducing SGML with water-immersion restraint stress (WIRS). After a comprehensive evaluation of serum and gastric tissue indicators in rats, 16S rRNA sequencing and metabolomics analyses were conducted to assess the impact of HP P-C on the fecal microorganisms and metabolites and their correlation. Results: Animal experiment suggested that pretreatment with HP P-C effectively reduced the gastric mucosal lesions, remarkably increased superoxide dismutase (SOD) activity in SGML model rats induced by WIRS. 16S rRNA sequencing analysis showed that HP P-C altered the composition of gut microbiota by raising the abundance of Lactobacillus and Akkermansia . In addition, metabolomics data identified seventeen main differential metabolites related to WIRS-induced gastric mucosal injury, primarily involving in tyrosine metabolism and betalain biosynthesis. HP P-C was found to regulate tyrosine metabolism and betalain biosynthesis by down-regulating the tyramine, L-tyrosine and L-dopa and up -regulating the gentisic acid and dopaquinone. Conclusion: Taken together, this study indicated that HP P-C could effectively protect against WIRS-induced gastric mucosal lesions by modulating intestinal flora and metabolites.
Abstract The education programs for transesophageal echocardiography (TEE), which plays significant roles in various surgical procedures, in China is currently limited to stimulation system and in-hospital patients. Although, existing TEE simulators have become increasingly sophisticated, the use of animal models retains significant advantages in terms of enabling dynamic cardiac monitoring. The aim of this study is to develop a better teaching and training program for TEE and establish baseline values for the porcine animal model. The thorough TEE examinations were conducted in 10 domestic porcine weighing 45-60kg according to ASA guideline for TEE. All the recommended views were explored and saved. The depth and angles of each view were recorded. Additionally, hemodynamic measurements were performed and recorded. All parameters were compared with human reference values. The porcine model is feasible to initiate a teaching model, and TEE baseline values were acquired for more development in the experimental porcine model.
Marine bacteria are a rich source of potentially useful antimicrobial molecules. The purpose of the study is to explore the diversity of bacteria with antimicrobial activity isolated from Siganus fuscescens gastrointestinal tract collected from Naozhou Island (20 degrees 52' N-20 degrees 56' N 110 degrees 33' E-110 degrees 38' E), Leizhou Bay, South China Sea.We isolated bacteria from the gastrointestinal tract of fish sample using classical culturing technique, and determined antimicrobial activities of the isolates by Oxford cup method. We investigated diversity of antimicrobial isolates using phylogenetic comparative analysis of 16S rRNA gene sequences.According to the results of morphological observation and part of physiological and biochemical experiments, we isolated 68 strains from fish gastrointestinal tract. Among them, 19 strains with antimicrobial activities were acquired (27.9% of the isolates) and represented 19 different species, belonging to 12 genera (Rothia, Micrococcu, Brachybacterium, Brevibacterium, Psychrobacter, Paracoccus, Cobetia, Citrobacter, Pseudomonas, Bacillus, Lactobacillus and Staphylococcus) of 11 families (Microbacteriaceae, Dermabacteraceae, Brevibacteriaceae, Moraxellaceae, Rhodobacteraceae, Halomonadaceae, Enterobacteriaceae, Pseudomonadaceae, Bacillaceae, Lactobacillaceae and Staphylococcaceae) in three phyla (Actinobacteria, Proteobacteria and Firmicutes). Eight strains (42.1%), 7 strains (36.8%) and 4 strains (21.1%) were belonging to the phylum Firmicutes, Proteobacteria and Actinobacteria, respectively. The phylogenetic distance matrix results suggested that there were obvious genetic divergences between the majority of strains with antimicrobial activity and their phylogenetically most closely related typical strain, due to 16S rRNA gene sequences similarities ranging from 96.2 to 99.9%. In addition, 4 strains (ZJHD2-31, ZJHD5-23, ZJHD2-58 and M26) could represent potential new species, and identification of the novel strain M26 has been published in Antonie van Leeuwenhoek.There are abundant diversity for bacteria with antimicrobial activity and potentially more new species of microorganism in Siganus fuscescens gastrointestinal tract collected from Naozhou Island.
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