The role of a mother in the immunization program is very important, so their understanding of immunization is necessary, the behavior of a person or society about health is determined by knowledge, attitude, belief, tradition, work etc. from the person or society concerned. In addition, the availability of health care facilities, attitudes, behavior of health workers on the provision of health information will also support and strengthen the formation of health behaviors, This study aims to determine the factors that affect the provision of complete basic immunization, This study uses a combined research method between quantitative and qualitative.Sample for this study is 107 mother mother, while informant for qualitative study is Head of PuskesmasPengambiran Padang City, Responsible, Immunization Officer of Puskesmas and Kader which is in Working Area of PengambiranPublic Health in Padang City, The result of this study shown that family support is the most important factors in the implementation Complete basic immunization (p = 0.001). The results of the analysis revealed that the coverage of the implementation of complete basic immunization has not been successful because the lack of active role of cadres and health officers in community in informing immunization in depth. so that there is still a lack of understanding from mothers and families which has no family support in the implementation of complete basic immunization, it has not reached 95% target because the system implementation of complete basic immunization services that have not been optimal. It needs various efforts to improve the achievement of complete basic immunization coverage especially through optimizing the role and function of health and cadre officers and optimizing partnerships with various parties.
Background: Acne vulgaris (AV) is a follicular disease that occurs most often and affects the area containing sebaceous gland follicles, including the face, back, and body. This is a multifactorial condition. The prevalence of acne vulgaris (AV) is still high both in the world and in Indonesia. As many as 80% of AV is found at the age of 11-30 years and almost 100% in adolescents. Various factors can trigger the onset of AV which can lead to severe complications. Aim: This study looked for the difference in interleukin-8 (IL-8) on degrees of AV severity. Materials and method: This study was an observational analytic study with a crosssectional approach. The research was conducted at the Diniyah Putri Islamic Boarding School, Lampung Province, Indonesia, in August 2020. The independent variable in this study was the AV degree, while the dependent variable was the IL-8 level. The sampling method in this study was consecutive sampling, as many as 63 AV patients. The research material is serum from venous blood. Examination of interleukin-8 levels using the ELISA method. Data analysis was univariate to find the mean and standard deviation of IL-8 levels, as well as the frequency distribution to determine the degree of AV. While the bivariate analysis was to determine the difference in the mean of IL-8 levels in AV patients. Results: The mean of IL-8 levels in AV patients was 38.53. Most of the AV patients are in a severe category. Further analysis found no difference in the mean of IL-8 levels between moderate and severe AV degrees. Conclusion: There was no difference in the mean of IL-8 levels in moderate and severe AV patients, but the IL-8 levels at severe AV degrees were higher than moderate AV degrees.
: Objective: The aim with the present study was to determine that arginine affect to the level of IL-6, IL-17 and TGF-β in high-fat diet-induced hypercholesterolemia in rats. Materal and Methods: Male Wistar rats in eight weeks old (n=35) were divided into five groups, one group as a control (C) were given standard r fed and four groups were given high-fat diet (Hfd) with fats from beef tallow. One group of high-fat diet rats recieved no arginine, three groups were recieved arginine in doses of 100, 200 and 400 mg/kg/day each for three weeks. After two weeks of treatment, blood cholesterol were measured, while serum level of IL-6, IL-17 and TGF-β were measured at the final five weeks of this protocol by ELISA methods. Results: There were no significantly different between groups, which received different doses of arginine (p>0,05). IL-17 of high-fat diet received arginine of doses 100 and 200 mg/kg/day were significantly higher compared to high-fat diet received no arginine and control group. Interestingly, IL-17 in high-fat diet group received arginine of doses 400 mg/kg/day were lower compared to groups received lower doses of arginine. TGF-β levels in the high-fat diet group's recieved arginine were lower compared to control group. Conclusions: The effect of arginine on cytokines plays the role on vascular inflammation due to high-fat diet.Key Words: Arginine, High-fat diet, IL-6, IL-17, and TGF–β.
Escherichia coli (E. coli) merupakan salah satu jenis bakteri penyebab terbanyak vaginitis aerobik yang dapat memberikan dampak buruk bagi kesehatan wanita hamil dan janin. Infeksi E. coli yang resisten terhadap antibiotik yang umum digunakan dapat mempersulit, mengganggu, dan memberikan hasil terapi yang buruk. Bakteri E. coli diketahui mampu bertindak sebagai donor dan resipien gen resistensi melalui mekanisme konjugasi, transformasi, dan transduksi. Tujuan penelitian ini adalah menguji kemampuan bakteriosin dari Lactobacillus crispatus (L. crispatus) sebagai senyawa antimikroba yang dapat meminimalisir pengembangan resistensi antibiotik pada bakteri E. coli. Bakteriosin dari Lactobacillus crispatus diperoleh melalui proses sentrifugasi dan filtrasi menjadi bentuk Cell-Free Supernatant. Penelitian ini dilakukan pada bulan Juli – Desember 2023 di Laboratorium Mikrobiologi dan Laboratorium Pusat Diagnostik dan Riset Penyakit Infeksi Fakultas Kedokteran Universitas Andalas. Sampel penelitian ini adalah pathogenic E. coli yang diperoleh dari swab vagina. Penelitian dilakukan dengan teknik dilusi dan Real Time PCR dengan desain penelitian rancangan acak lengkap. Cell Free Supernatant dibagi menjadi konsentrasi 100%, 80%, 60%, 40%, 20%, dan 0% (kontrol). Nilai KHM dan KBM diperoleh dari jumlah bakteri yang tumbuh dan terdeteksi melalui Real Time PCR pada masing-masing konsentrasi. Hasil penelitian diolah secara statistik dengan uji One Way ANOVA dan Post Hoc LSD. Hasil penelitian diperoleh bahwa bacteriosin-containing Cell-Free Supernatant dari Lactobacillus crispatus tidak memiliki efek bakteriostatik dan bakterisidal terhadap pathogenic Escherichia coli dengan nilai KHM dan KBM yang tidak dapat diidentifikasi.
AbstrakDi kota Padang cakupan imunisasi sebesar 88,1% dengan cakupan terendah di wilayah kerja Puskesmas Lubuk Buaya sebesar 81,8%. Serta Kelurahan Parupuk Tabing merupakan kelurahan yang cakupannya terendah dan angka drop-out tertinggi sebesar 12,9% di tahun 2012. Penelitian ini bertujuan untuk mengetahui hubungan tingkat pengetahuan ibu dengan pemberian imunisasi dasar lengkap bayi di wilayah kerja Kelurahan Parupuk Tabing Wilayah Kerja Puskesmas Lubuk Buaya Kota Padang Tahun 2013.Metode: Jenis penelitian ini analitik dengan desain cross-sectional. Populasi adalah ibu yang memiliki bayi usia 1-2 tahun di Kelurahan Parupuk Tabing. Jumlah sampel 63 orang diambil secara Random Sampling. Data dikumpulkan melalui wawancara menggunakan kuesioner dan observasi.pengolahan data dilakukan secara komputerisasi dengan analisis uji Chi-Square pada α=0,05. Didapatkan 57,1%, responden memberikan imunisasi dasar lengkap pada bayinya dan 63,5%. responden yang mempunyai pengetahuan yang cukup tentang imunisasi dasar lengkap. Didapatkan adanya hubungan bermakna antara kedua variabel tersebut.Kesimpulan: terdapat hubungan yang bermakna antara pengetahuan ibu terhadap pemberian imunisasi dasar lengkap pada bayi di kelurahan Parupuk Tabing wilayah kerja puskesmas Lubuk Buaya. Sehingga disarankan kepada kader posyandu dan petugas puskesmas agar memberikan penyuluhan tentang imunisasi, fungsi, dan jadwal pemberian imunisasi tersebut.Kata kunci: imunisasi dasar lengkap, pengetahuanAbstractIn the city of Padang immunization coverage was 88.1% with the lowest coverage in the Lubuk Buaya Public Health Centre was 81.8 % .Village of Parupuk Tabing was the lowest coverage and highest drop-out rate of 12.9% in 2012. This study aims to determine relationshipof the level of mother's knowledge with base complete infant immunization in the Village of Parupuk Tabing Lubuk Buaya Public Health Centre working area in the City of Padang in 2013. Type of this study is analytic study in the form of a cross - sectional design. The population of this study is all of mothers with babies aged 1-2 years in the Village of Parupuk Tabing. The number of samples taken 63 Random Sampling. Data were collected by interviews using questionnaires.Computerized data processing and analisis perfomed by Chi - Square test at α = 0.05. Obtained 57.1% of respondents are fully immunized and 63.5 % of the respondents have sufficient knowledge about the complete basic immunization. There was a significant correlation between the two variables (p=0,000). There was a significant relationship between mother's knowledge to complete basic immunization in infants in Village of Parupuk Tabing Lubuk Buaya Public Health Centre working area. So it is advisable to officer cadre of health posts and health centers that provide counseling about immunization, function, and the immunization schedule.Keywords:complete basic immunization, knowledge
Bone marrow mesenchymal stem cells (BM-MSCs) increases the percentage of integrin α2β1 expression on rat’ wound healing process, but the study which is related to PBMC (peripheral blood mononuclear cell) and PBMC combined with BM-MSCs towards burn healing process has not been conducted yet. The aim of the study was to determine the expression of integrin α2β1 as a result of PBMC and PBMC combined to BM-MSCs administrations towards rat’ wound healing process. Experimental research with the post test only control design was conducted on 12 Wistar rats, divided into three experimental groups: PBS (Phosphate Buffer Saline) as control group, PBMC, and PBMC combined with BM-MSCs. Stem cells were subcutaneously injected with 2x106 cells/ml. A full-thickness burn was made on the dorsal side (back). Skin tissues were collected to investigate the expression of integrin α2β1 using immunohistochemistry method on day-14. Administration of only PBMC (2.972), and PBMC combined with BM-BSCs (3.297) elevated the percentage of integrin α2β1 compared with control group (2.340), but significant result was only in combination group (p=0,05). The administration of only PBMC and PBMC combined with BM-MSCs elevated the expression of integrin α2β1, which showed that both of stem cells accelerated the migration of keratinocyts that will expedite the re-epithelialization phase in burn wound healing.
Background: Migraine is a primary headache disorder that ranks as the third leading cause of disability. Various prophylactic therapies have been developed for migraine treatment, including vitamin D3 supplementation. The mechanism of action of vitamin D3 supplementation in the pathophysiology of migraine has not been extensively studied. Objective: This study assesses the impact of vitamin D3 administration on the levels of the biomarkers CGRP, Glutamate, and NLRP3 and its effect on reducing the frequency and intensity of migraine attacks in chronic migraine patients. Methods: This experimental study (single-blind clinical trial) observes two groups: a group given vitamin D3 at 2000 IU for 12 weeks and a placebo group. This study involved 61 chronic migraine subjects. At the end of the study, after excluded 31 subjects, there were 12 subjects in each of the two groups. Results: A significant relationship was found between vitamin D3 administration and the reduction in the frequency and duration of migraine attacks in both the vitamin D3 group (p<0.001) and the placebo group (p=0.078). No significant relationship was found between vitamin D3 administration and changes in CGRP levels (p=0.633), but there were significant changes in glutamate (p<0.001) and NLRP3 (p=0.016) levels following vitamin D3 administration. Conclusion: Vitamin D3 supplementation has an impact on reducing the frequency and duration of migraine attacks in chronic migraine patients, and there is a significant association with changes in glutamate and NLRP3 levels, but not with changes in CGRP levels.
