N Simiónescu

Romanian Academy

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Co-Authors

Maya Simionescu

Romanian Academy

M Scherzer

Charité - Universitätsmedizin Berlin

Anca Roxana Petrovici

Romanian Academy

Anca V. Sima

Romanian Academy

George E. Palade

Universitatea de Medicină, Farmacie, Științe și Tehnologie „George Emil Palade” din Târgu Mureș

Loredan S. Niculescu

Romanian Academy

Mariana Pinteală

Romanian Academy

M Oprescu

Spitalul Clinic Judetean de Urgentã Craiova

Mihail Coculescu

Carol Davila University of Medicine and Pharmacy

A Constantinovici

Clinical Emergency Hospital Bucharest

Cooperative Institutions

Manchester Metropolitan University

133

Romanian Academy

University of Salford

Grigore T. Popa University of Medicine and Pharmacy

Universidad de Granada

Clinical Emergency Hospital Bucharest

Istituti di Ricovero e Cura a Carattere Scientifico

Carol Davila University of Medicine and Pharmacy

Centro Cardiologico Monzino

Institutul de Chimie Macromoleculară Petru Poni

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Cytochemical dynamics of the adrenal medulla in experimental haemorrhagic shock.

PubMed (1961)

N Simiónescu M Goldstein

Medulla

Haemorrhagic shock

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Citations (1)

New Insights on Hemp Oil Enriched in Cannabidiol: Decarboxylation, Antioxidant Properties and In Vitro Anticancer Effect

Antioxidants (2021)

Anca Roxana Petrovici N Simiónescu Andreea-Isabela Sandu Vasile Paraschiv Mihaela Silion

This study aimed to obtain and characterize extracted hemp oil enriched in cannabidiol (CBD) by decarboxylation of cannabidiolic acid (CBDA) and to give new insights into its antioxidant and anticancer effects. Optimization of CBDA decarboxylation in hemp oil was performed, and CBD and CBDA contents and purities were determined by flash chromatography, 1H- and 13C-NMR. The antioxidant properties of CBD-enriched oil were investigated by Fe2+ chelating activity, Fe3+ reducing antioxidant power assay, O2●− scavenging activity, HO● scavenging ability and lipid peroxidation inhibitory assay, and its cytotoxicity, apoptosis- and oxidative stress-inducing effects on NHDF, MeWo, HeLa, HepG2 and HOS cells were determined. The CBD concentration in hemp oil was increased by CBDA soft decarboxylation optimized at 90 °C, for 1 h and the resulting oil was capable of reducing iron, scavenging free radicals and inhibiting lipid peroxidation in cell-free oxidative conditions. CBD-enriched oil promoted NHDF proliferation at up to 15 µg CBD/mL, while inducing apoptosis and ROS production and modulating antioxidant enzymes’ gene expression in cancer cells, being selective for osteosarcoma cells, and induced apoptosis by p53- and ROS-independent mechanisms. CBD-enriched hemp oil demonstrated antioxidant properties in oxidative conditions and promoted normal fibroblasts’ proliferation, while inducing apoptosis and ROS production in cancer cells.

10.3390/antiox10050738

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Citations (44)

[Acute cerebral edema, an aggravating factor in multiple trauma in children].

PubMed (1988)

Ciurea Av A Arseni D Alexianu N Simiónescu C Drozan

Aggravating Factor

Brain Edema

Cerebral edema

Cerebral trauma

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Citations (0)

Micro vascular endothelium segmental differentiations transcytosis and selective distribution of anionic sites

N Simiónescu

Transcytosis

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Citations (4)

Differentiated micro domains on the luminal surface of the capillary endothelium

Biorheology (1981)

G.F. Palade Maya Simionescu N Simiónescu

The distribution of anionic sites on the luminal surface of the fenestrated endothelium of intestinal and pancreatic blood capillaries was investigated using cationized ferritin (CF), pI 8.4, as a probe. CF was administered in vivo by i.v. injection,

Unmanned surface vehicle

10.3233/bir-1981-183-619

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Citations (58)

Endothelial Albumin Binding Proteins Are Membrane-associated Components Exposed on the Cell Surface

Journal of Biological Chemistry (1989)

Nicolae Ghinea M Eskenasy Maya Simionescu N Simiónescu

The heterobifunctional, photoactivatable, thiol-cleavable cross-linker sulfosuccinimidyl 2-(p-azido-salicylamido)ethyl-1,3′-dithiopropionate (SASD) was radioiodinated and used to determine whether endothelial albumin binding proteins (ABP) recently identified (Ghinea, N., Fixman, A., Alexandru, D., Popov, D., Hasu, M., Ghitescu, L., Eskenasy, M., Simionescu, M., and Simionescu, N. (1988) J. Cell Biol. 107, 231–239) are plasma membrane-associated components exposed on the cell surface. Microvascular endothelial cells (MEC) freshly isolated from rat epididymal fat were incubated with 125I-2-(p-azidosalicylamido)ethyl-1,3′-dithiopropionate (ASD)-albumin conjugate which upon photolysis by UV light was cross-linked to the receptor proteins. By cleaving the disulfide linkages of the cross-linker with 5% β-mercaptoethanol and the ligand-receptor interactions with 0.1% sodium dodecyl sulfate, the radioiodinated ASD moiety remained attached to the receptor peptides which were further detected by 5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by autoradiography. In parallel, samples were examined by ligand blotting with albumin-gold complex. The results showed that in these experimental conditions ABP are represented by two major peptides of 31 and 18 kDa and two minor bands of 73 and 56 kDa. Densitometric scanning showed that the two major bands constitute more than 70% of the total ABP. The four peptides were not apparent if the samples were not UV-irradiated. The binding of the radioiodinated ligand to ABPs was reduced by ∼ 82% in the presence of excess competitive unlabeled albumin. When MEC were incubated with unlabeled SASD and exposed to UV light, the autoradiographic banding pattern obtained was similar to that of either radioiodinated receptor proteins or MEC not treated with SASD. This indicated that the four albumin binding peptides are distinct proteins of the endothelial cell plasmalemma.

Sodium dodecyl sulfate

Linker

Cell surface receptor

Moiety

Serum Albumin

10.1016/s0021-9258(18)83652-3

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Citations (83)

[Amyloid goiter with increased thyroid function in a patient with pulmonary tuberculosis].

PubMed (1962)

I Juvara N Simiónescu Dana Redulescu

Amyloid (mycology)

Source

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Citations (1)

[Experimental surgical models in endocrinology].

PubMed (1966)

N Simiónescu

Source

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Citations (0)

The hyperlipidemic and diabetic hamster, a novel animal model

Atherosclerosis (1994)

Maya Simionescu Doina Popov Anca V. Sima M. Hasu G Costache

Hyperlipidemia

10.1016/0021-9150(94)93328-6

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Citations (2)

Open junctions in the endothelium of the postcapillary venules of the diaphragm.

The Journal of Cell Biology (1978)

N Simiónescu Maya Simionescu George E. Palade

We have previously established that approximately 30% of the endothelial junctions in the pericytic venules of the mouse diaphragm are open to a gap of approximately 30--60 A, and are fully permeated by hemeundecapeptide (H11P) (mol diam approximately 20 A). To estimate the size limit for molecules that can permeate these junctions, we have administered graded tracers intravenously and studied their behavior at the level of pericytic venules in bipolar microvascular fields (BMFs) in the mouse diaphragm. Horseradish peroxidase (HRP) (mol diam approximately 50 A) permeated only approximately 50% of the open junctions of the venular endothelium. Outflow through venular junctions appeared to be modest since the tracer remained restricted to the perivenular spaces. Hemoglobin (Hb, mol diam 64 x 55 x 50 A) permeated only a few (less than 5%), and ferritin (mol diam 110 A), practically none, of the endothelial junctions of the pericytic venules. The findings suggest that under normal conditions the size limit for permeant molecules for open venular junctions is approximately 60 A. Replicas of freeze-fracture preparations from appropriate regions in BMF showed that the intercellular junctions of the venular endothelium have the same organization as previously described for the corresponding segments of the microvasculature in the omentum and mesentery: discontinuous creases or grooves either free of or marked by few intramembrane particles only. Administration of histamine (topically or systemically) and 5-hydroxytryptamine (5-HT) (topically) resulted in typical focal separations of the endothelial junctions and intramural deposits of large tracer particles (carbon black) in the postcapillary venules.

Horseradish peroxidase

10.1083/jcb.79.1.27

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Citations (177)