Jesús V. Jorrín–Novo

University of Córdoba

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Ana M. Maldonado‐Alconada

University of Córdoba

MÁ

María Ángeles Castillejo

University of Córdoba

Luís Valledor

Universidad de Oviedo

María-Dolores Rey

Le Mans Université

M. Cristina Romero‐Rodríguez

Universidad Nacional de Asunción

José Valero-Galván

Universidad Autónoma de Ciudad Juárez

Víctor M. Guerrero-Sánchez

University of Córdoba

Besma Sghaier‐Hammami

University of Carthage

Diego Rubiales

Instituto de Agricultura Sostenible

Rosa Sánchez‐Lucas

University of Birmingham

Cooperative Institutions

University of Córdoba

141

Consejo Superior de Investigaciones Científicas

Cordoba University

Instituto de Agricultura Sostenible

Centre National de la Recherche Scientifique

Universidad Autónoma de Madrid

Universidad de Oviedo

Consejo Nacional de Investigaciones Científicas y Técnicas

University of Naples Federico II

Universidad Complutense de Madrid

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Improving the quality of protein identification in non-model species. Characterization of Quercus ilex seed and Pinus radiata needle proteomes by using SEQUEST and custom databases

Journal of Proteomics (2014)

M. Cristina Romero‐Rodríguez Jesús Pascual Luís Valledor Jesús V. Jorrín–Novo

UniProt

Pinus radiata

Identification

False Discovery Rate

10.1016/j.jprot.2014.01.027

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Citations (56)

Editorial: International Plant Proteomics Organization (INPPO) World Congress 2014

Frontiers in Plant Science (2016)

Joshua L. Heazlewood Jesús V. Jorrín–Novo Ganesh Kumar Agrawal Silvia Mazzuca Sabine Lüthje

EDITORIAL article Front. Plant Sci., 05 August 2016Sec. Plant Proteomics and Protein Structural Biology Volume 7 - 2016 | https://doi.org/10.3389/fpls.2016.01190

Plant science

10.3389/fpls.2016.01190

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Application of Label-Free Shotgun nUPLC–MS^E and 2-DE Approaches in the Study of Botrytis cinerea Mycelium

Journal of Proteome Research (2013)

Raquel González-Fernández Kerman Aloria Jesús M. Arizmendi Jesús V. Jorrín–Novo

The phytopathogenic fungus Botrytis cinerea infects more than different 200 plant species and causes substantial losses in numerous crops. The B05.10 and T4 wild-type strain genomes have been recently sequenced, becoming a model system for necrotrophic pathogens, as well as opening up new alternatives in functional genomics, such as proteomics. We analyzed B. cinerea mycelium from these two wild-type strains, introducing label-free shotgun nUPLC-MS(E) methodology to complement the 2-DE-MS-based approach. We assessed the label-free nUPLC-MS(E) methodology for protein identification and quantification using five mycelium protein dilutions. A total of 225 and 170 protein species were identified by nUPLC-MS(E) in the B05.10 and T4 strains, respectively. Moreover, 129 protein species were quantified in both strains. Significant differences in protein abundance were found in 15 more abundant and 16 less abundant protein species in the B05.10 strain compared to the T4 strain. Twenty-nine qualitative and 15 significant quantitative differences were found using 2-DE. The label-free nUPLC-MS(E) was a reliable, reproducible and sensitive method for protein identification and quantification to study the B. cinerea mycelial proteome. Results obtained by gel-based and gel-free complementary approaches allow a deeper characterization of this fungus, as well as the identification of potential virulence factors.

Shotgun proteomics

Shotgun

Proteome

10.1021/pr3010937

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La utilización de inductores de resistencia sistémica adquirida en el control de plantas parásitas: una hipótesis

E. Prats Jesús V. Jorrín–Novo Diego Rubiales Olmedo

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Metabolism of Chlorotoluron in Resistant and Susceptible Alopecurus Myosuroides. Biotypes. a Study Using Plant Cell Suspensions

Springer eBooks (1997)

J. Menéndez Jesús V. Jorrín–Novo Rafael De Prado

Avena fatua

Lolium rigidum

Phytotoxicity

Allelopathy

10.1007/978-94-011-5538-0_18

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Citations (1)

Proteomics research in forest trees: A 2012-2022 update

Frontiers in Plant Science (2023)

María Ángeles Castillejo Jesús Pascual Jesús V. Jorrín–Novo Tiago Santana Balbuena

This review is a compilation of proteomic studies on forest tree species published in the last decade (2012-2022), mostly focused on the most investigated species, including Eucalyptus, Pinus , and Quercus . Improvements in equipment, platforms, and methods in addition to the increasing availability of genomic data have favored the biological knowledge of these species at the molecular, organismal, and community levels. Integration of proteomics with physiological, biochemical and other large-scale omics in the direction of the Systems Biology, will provide a comprehensive understanding of different biological processes, from growth and development to responses to biotic and abiotic stresses. As main issue we envisage that proteomics in long-living plants will thrive light on the plant responses and resilience to global climate change, contributing to climate mitigation strategies and molecular breeding programs. Proteomics not only will provide a molecular knowledge of the mechanisms of resilience to either biotic or abiotic stresses, but also will allow the identification on key gene products and its interaction. Proteomics research has also a translational character being applied to the characterization of the variability and biodiversity, as well as to wood and non-wood derived products, traceability, allergen and bioactive peptides identification, among others. Even thought, the full potential of proteomics is far from being fully exploited in forest tree research, with PTMs and interactomics being reserved to plant model systems. The most outstanding achievements in forest tree proteomics in the last decade as well as prospects are discussed.

10.3389/fpls.2023.1130665

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Citations (7)

Proteome regulation and epigenetic code during Pinus radiata needle maturation

Proteómica: revista de la Sociedad Española de Proteómica (2010)

Luís Valledor María Jesús Cañal Christof Lenz Roberto Rodríguez Madrera Jesús V. Jorrín–Novo

the differential expression levels of histone epigenetic marks, being the levels of acetylated Histone H4 and Histone H3K4, associated to gene expression, higher in immature needles whereas the Histone H3K9 was only found in mature needles (Figure 1c). This could be explained by the fact that chromatin needs to be altered and restructured during the differentiation to regulate gene expression [1].

Histone code

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Bioquímica Vegetal y Agrícola-Proteómica Vegetal. Universidad de Córdoba

Proteómica: revista de la Sociedad Española de Proteómica (2007)

Jesús V. Jorrín–Novo Ana M. Maldonado‐Alconada

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Otolicuorrea espontánea en el adulto. Presentación de dos casos y revisión de la literatura

Neurocirugía (2011)

Lara Galbarriatu J. Aurrecoechea E. Ruiz de Gopegui Iñigo Pomposo G. Bilbao

Middle fossa

10.1016/s1130-1473(11)70013-6

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Citations (3)

Negative cross‐resistance to bentazone and pyridate in atrazine‐resistant Amaranthus cruentus and Amaranthus hybridus biotypes

Pesticide Science (1992)

Rafael De Prado Manuel Sánchez Jesús V. Jorrín–Novo Carmen Domínguez

Abstract Plants of Amaranthus cruentus and Amaranthus hybridus resistant to atrazine and cyanazine were found in maize fields in north‐eastern Spain. Both resistant foiotypes survived doses of 5 kg ha −1 of atrazine and 2–4 kg ha −1 of cyanazine but were controlled by lower doses of bentazone and pyridate than were susceptible biotypes. Such a negative cross‐resistance was not found for chloroacetamides and MCPA. Chlorophyll fluorescence studies revealed that atrazine, bentazone, cyanazine and pyridate (10 mg litre −1 ) caused inhibition of photosynlhetic electron transport in susceptible leaves, while in resistant plants, atrazine and cyanazine had no effect. Conversely, bentazone and pyridate inhibited photosynthesis to a greater extent in resistant than in susceptible biotypes. Isolated chloroplast membranes from resistant biotypes showed resistance factors of 366 and 501 to atrazine and 39 and 60 to cyanazine for A. hybridus and A. cruentus , respectively. Bentazone and pyridate were found to be more effective in chloropiasts of the resistant biotypes than those of the susceptible plants. It is suggested that enhanced susceptibility to bentazone and pyridate in triazine‐resistant A. cruentus and A. hybridus biotypes may be associated with the alteration of the D‐I polypeptide subunit of photosystem II, as found in triazine‐resistant plants.

Amaranthus hybridus

Amaranthus cruentus

Amaranthaceae

Abutilon

10.1002/ps.2780350206

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Citations (31)