Jesús V. Jorrín–Novo

University of Córdoba

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Ana M. Maldonado‐Alconada

University of Córdoba

MÁ

María Ángeles Castillejo

University of Córdoba

Luís Valledor

Universidad de Oviedo

María-Dolores Rey

Le Mans Université

M. Cristina Romero‐Rodríguez

Universidad Nacional de Asunción

José Valero-Galván

Universidad Autónoma de Ciudad Juárez

Víctor M. Guerrero-Sánchez

University of Córdoba

Besma Sghaier‐Hammami

University of Carthage

Diego Rubiales

Instituto de Agricultura Sostenible

Rosa Sánchez‐Lucas

University of Birmingham

Cooperative Institutions

University of Córdoba

141

Consejo Superior de Investigaciones Científicas

Cordoba University

Instituto de Agricultura Sostenible

Centre National de la Recherche Scientifique

Universidad Autónoma de Madrid

Universidad de Oviedo

Consejo Nacional de Investigaciones Científicas y Técnicas

University of Naples Federico II

Universidad Complutense de Madrid

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Release of phytoalexins and related isoflavonoids from intact chickpea seedlings elicited with reduced glutathione at root level

Plant Physiology and Biochemistry (2001)

José Luis Platero Armero Raquel Requejo-Aguilar Jesús V. Jorrín–Novo Rafael López‐Valbuena Manuel Tena

Phenylpropanoid

Phytoalexin

Isoflavonoid

Biochanin A

Buthionine sulfoximine

10.1016/s0981-9428(01)01299-2

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Citations (45)

Why Consumers Prefer Green Friariello Pepper: Changes in the Protein and Metabolite Profiles Along the Ripening

Frontiers in Plant Science (2021)

Maria Tartaglia Rosaria Sciarrillo Daniela Zuzolo Angela Amoresano Anna Illiano

Fruit ripening is a physiologically complex process altering texture, color, flavor, nutritional value, and aroma. However, some fruits are consumed at an early stage of ripening due to the very peculiar characteristics varying during ripening. An example is a particular ecotype of pepper, the Friariello pepper, among the most important representatives of Campania (Southern Italy) agro-alimentary culture. In this study, for the first time, the physiological variations during Friariello ripening (green, veraison, and fully ripe) were evaluated by hyphenated mass spectrometric techniques in a proteomic and metabolomic approach. We found that Lutein and Thaumatin are particularly abundant in the green Friariello. Friariello at an early stage of ripening, is rich in volatile compounds like butanol, 1 3 5-cycloheptatriene, dimethylheptane, α-pinene, furan-2-penthyl, ethylhexanol, 3-carene, detected by gas chromatography-mass spectrometry (GC-MS) analysis, which give it the peculiar fresh and pleasant taste. The detected features of Friariello may justify its preferential consumption in the early ripening stage and outline new knowledge aimed at preserving specific agro-cultural heritage.

Veraison

Flavour

Cheese ripening

10.3389/fpls.2021.668562

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Citations (6)

Aproximaciones metodológicas para el estudio del estado redox (tiol disulfuro) de proteínas en muestras vegetales

Proteómica: revista de la Sociedad Española de Proteómica (2010)

Sira Echevarría‐Zomeño Per Hägglund P.Birte Svenson Ana M. Maldonado‐Alconada Jesús V. Jorrín–Novo

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Application and optimization of label-free shotgun approaches in the study of Quercus ilex

Journal of Proteomics (2021)

Mónica Escandón Jesús V. Jorrín–Novo María Ángeles Castillejo

Shotgun

Shotgun proteomics

10.1016/j.jprot.2020.104082

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Citations (8)

Proteomics Data Analysis for the Identification of Proteins and Derived Proteotypic Peptides of Potential Use as Putative Drought Tolerance Markers for Quercus ilex

International Journal of Molecular Sciences (2021)

Bonoso San-Eufrasio Ezequiel Darío Bigattón Víctor M. Guerrero-Sánchez Palak Chaturvedi Jesús V. Jorrín–Novo

Drought is one of the main causes of mortality in holm oak (Quercus ilex) seedlings used in reforestation programs. Although this species shows high adaptability to the extreme climate conditions prevailing in Southern Spain, its intrinsic genetic variability may play a role in the differential response of some populations and individuals. The aim of this work was to identify proteins and derived proteotypic peptides potentially useful as putative markers for drought tolerance in holm oak by using a targeted post-acquisition proteomics approach. For this purpose, we used a set of proteins identified by shotgun (LC-MSMS) analysis in a drought experiment on Q. ilex seedlings from four different provenances (viz. the Andalusian provinces Granada, Huelva, Cadiz and Seville). A double strategy involving the quantification of proteins and target peptides by shotgun analysis and post-acquisition data analysis based on proteotypic peptides was used. To this end, an initial list of proteotypic peptides from proteins highly represented under drought conditions was compiled that was used in combination with the raw files from the shotgun experiment to quantify the relative abundance of the fragment’s ion peaks with the software Skyline. The most abundant peptides under drought conditions in at least two populations were selected as putative markers of drought tolerance. A total of 30 proteins and 46 derived peptides belonging to the redox, stress-related, synthesis,-folding and degradation, and primary and secondary metabolism functional groups were thus identified. Two proteins (viz., subtilisin and chaperone GrpE protein) were found at increased levels in three populations, which make them especially interesting for validation drought tolerance markers in subsequent experiments.

Shotgun proteomics

Isobaric labeling

Shotgun

Proteome

10.3390/ijms22063191

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Citations (14)

Multiplex staining of 2-DE gels for an initial phosphoproteome analysis of germinating seeds and early grown seedlings from a non-orthodox specie: Quercus ilex L. subsp. ballota [Desf.] Samp.

Frontiers in Plant Science (2015)

M. Cristina Romero‐Rodríguez Nieves Abril Rosa Sánchez‐Lucas Jesús V. Jorrín–Novo

As a preliminary step in the phosphoproteome analysis of germinating seeds (0 and 24 h after seed imbibition) and early grown seedlings (216 h after seed imbibition) from a non-orthodox sp. Quercus ilex, a multiplex (SYPRO-Ruby and Pro-Q DPS) staining of high-resolution 2-DE gels was used. By using this protocol it was possible to detect changes in protein-abundance and/or phosphorylation status. This simple approach could be a good complementary alternative to the enrichment protocols used in the search for phosphoprotein candidates. While 482 spots were visualized with SYPRO-Ruby, 222 were with Pro-Q DPS. Statistically significant differences in spot intensity were observed among samples, these corresponding to 85 SYPRO-Ruby-, 20 Pro-Q-DPS-, and 35 SYPRO-Ruby and Pro-Q-DPS-stained spots. Fifty-five phosphoprotein candidates showing qualitative or quantitative differences between samples were subjected to MALDI-TOF-TOF MS analysis, with 20 of them being identified. Identified proteins belonged to five different functional categories, namely: carbohydrate and amino acid metabolism, defense, protein folding, and oxidation-reduction processes. With the exception of a putative cyclase, the other 19 proteins had at least one orthologous phosphoprotein in Arabidopsis thaliana, Medicago truncatula, N. tabacum, and Glycine max. Out of the 20 identified, seven showed differences in intensity in Pro-Q-DPS but not in SYPRO-Ruby-stained gels, including enzymes of the glycolysis and amino acid metabolism. This bears out that theory the regulation of these enzymes occurs at the post-translational level by phosphorylation with no changes at the transcriptional or translational level. This is different from the mechanism reported in orthodox seeds, in which concomitant changes in abundance and phosphorylation status have been observed for these enzymes.

Phosphoprotein

Imbibition

Medicago truncatula

Multiplex

10.3389/fpls.2015.00620

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Citations (12)

Differential expression proteomics to investigate responses and resistance to Orobanche crenata in Medicago truncatula

BMC Genomics (2009)

Ma Ángeles Castillejo Ana M. Maldonado‐Alconada Eliane E. Dumas-Gaudot Mónica Fernández‐Aparicio Rafael Susín

Parasitic angiosperm Orobanche crenata infection represents a major constraint for the cultivation of legumes worldwide. The level of protection achieved to date is either incomplete or ephemeral. Hence, an efficient control of the parasite requires a better understanding of its interaction and associated resistance mechanisms at molecular levels. In order to study the plant response to this parasitic plant and the molecular basis of the resistance we have used a proteomic approach. The root proteome of two accessions of the model legume Medicago truncatula displaying differences in their resistance phenotype, in control as well as in inoculated plants, over two time points (21 and 25 days post infection), has been compared. We report quantitative as well as qualitative differences in the 2-DE maps between early- (SA 27774) and late-resistant (SA 4087) genotypes after Coomassie and silver-staining: 69 differential spots were observed between non-inoculated genotypes, and 42 and 25 spots for SA 4087 and SA 27774 non-inoculated and inoculated plants, respectively. In all, 49 differential spots were identified by peptide mass fingerprinting (PMF) following MALDI-TOF/TOF mass spectrometry. Many of the proteins showing significant differences between genotypes and after parasitic infection belong to the functional category of defense and stress-related proteins. A number of spots correspond to proteins with the same function, and might represent members of a multigenic family or post-transcriptional forms of the same protein. The results obtained suggest the existence of a generic defense mechanism operating during the early stages of infection and differing in both genotypes. The faster response to the infection observed in the SA 27774 genotype might be due to the action of proteins targeted against key elements needed for the parasite's successful infection, such as protease inhibitors. Our data are discussed and compared with those previously obtained with pea [1] and transcriptomic analysis of other plant-pathogen and plant-parasitic plant systems.

Medicago truncatula

Proteome

Orobanche

Parasitic plant

Spots

Medicago

10.1186/1471-2164-10-294

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Citations (47)

Back to the basics: Maximizing the information obtained by quantitative two dimensional gel electrophoresis analyses by an appropriate experimental design and statistical analyses

Journal of Proteomics (2010)

Luís Valledor Jesús V. Jorrín–Novo