María Jesús Perteguer

Instituto de Salud Carlos III

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Teresa Gárate

Instituto de Salud Carlos III

Carmen Cuéllar

Universidad Complutense de Madrid

Florencio M. Ubeira

Universidade de Santiago de Compostela

Marta González–Warleta

Xunta de Galicia

Ana Hernández-González

University of California, Davis

Victoria Martínez‐Sernández

Universidade de Santiago de Compostela

Mercedes Mezo

Xunta de Galicia

Fernanda Romarı́s

Universidade de Santiago de Compostela

Agustín Benito

Centro Nacional de Medicina Tropical

Zaida Herrador

Centro Nacional de Medicina Tropical

Cooperative Institutions

Instituto de Salud Carlos III

Centro Nacional de Microbiologia

Universidad Complutense de Madrid

Centro de Investigación Biomédica en Red

Hospital Universitario La Paz

Universidade de Santiago de Compostela

Consejo Superior de Investigaciones Científicas

Centro Nacional de Medicina Tropical

Centre for Biomedical Network Research on Rare Diseases

Universidad de Salamanca

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Aplicación de la técnica PCR-RFLP en la identificación de nematodos implicados en la anisakiasis humana

Medicina Clínica (2004)

María Jesús Perteguer Guadalupe Ortiz Elena G. Sánchez María Flores‐Chávez Esperanza Rodríguez

10.1157/13062165

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Citations (0)

Evidence for Suppression of Onchocerciasis Transmission in Bioko Island, Equatorial Guinea

PLoS neglected tropical diseases (2016)

Laura Moya Zaida Herrador Thuy‐Huong Ta‐Tang José Miguel Rubio María Jesús Perteguer

Onchocerciasis or "river blindness" is a chronic parasitic neglected tropical disease which is endemic both in mainland and insular Equatorial Guinea. We aim to estimate the current epidemiological situation of onchocerciasis in Bioko Island after vector elimination in 2005 and more than sixteen years of Community Directed Treatment with Ivermectin (CDTI) by using molecular and serological approaches for onchocerciasis diagnosis. A community-based cross-sectional study was carried out in Bioko Island from mid-January to mid-February 2014. A total of 544 study participants were recruited. A complete dermatological examination was performed and three skin snips were performed in every participant for parasitological and molecular assessments. Blood spots were also taken for determination of Ov16 IgG4 antibodies trough an "in-house" ELISA assay. Overall, we found 15 out of 522 individuals suffering any onchocerciasis specific cutaneous lesions and 16 out of 528 (3.0%) with onchocercal nodules in the skin. Nodules were significantly associated with age, being more common in subjects older than 10 years than in younger people (3.9% vs. 0%, p = 0.029). Regarding the onchocerciasis laboratory assessment, no positive parasitological test for microfilaria detection was found in the skin snips. The calculated seroprevalence through IgG4 serology was 7.9%. No children less than 10 years old were found to be positive for this test. Only one case was positive for Onchocerca volvulus (O. volvulus) after skin PCR. The present study points out that the on-going mass ivermectin treatment has been effective in reducing the prevalence of onchocerciasis and corroborates the interruption of transmission in Bioko Island. To our knowledge, this is the first time that accurate information through molecular and serological techniques is generated to estimate the onchocerciasis prevalence in this zone. Sustained support from the national program and appropriate communication and health education strategies to reinforce participation in CDTI activities are essential to ensure progress towards onchocerciasis elimination in the country.

Microfilaria

Loa loa

Lymphatic Filariasis

Mass drug administration

Tropical Medicine

10.1371/journal.pntd.0004829

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Citations (26)

Analysis of an expressed sequence tag library from Dicrocoelium dentriticum

Experimental Parasitology (2013)

Ana María Martínez-Ibeas María Jesús Perteguer Camino González Lanza Teresa Gárate M. Yolanda Manga-González

10.1016/j.exppara.2013.07.005

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Citations (6)

Comparison of T24H-his, GST-T24H and GST-Ts8B2 recombinant antigens in western blot, ELISA and multiplex bead-based assay for diagnosis of neurocysticercosis

Parasites & Vectors (2017)

Ana Hernández-González John Noh María Jesús Perteguer Teresa Gárate Sukwan Handali

Currently, the reference standard assay for the serodiagnosis of neurocysticercosis (NCC) is the lentil lectin-bound glycoproteins/enzyme-linked immunoelectrotransfer blot (LLGP-EITB). The main disadvantage of this technique is the complexity of obtaining and purifying the LLGP extract. This could be solved by replacement with highly specific recombinant antigens from Taenia solium. Based on previous studies, we selected and produced the recombinant Ts8B2 and T24H proteins and applied them to three diagnostic techniques: western blot (WB), enzyme-linked immunosorbent assay (ELISA) and the multiplex bead-based assay (MBA). The Ts8B2 and T24H cDNA sequences were expressed in a prokaryotic system and the corresponding expression products purified; three recombinant proteins were further characterized: T24H-his, GST-T24H and GST-Ts8B2. The proteins on WB, ELISA and MBA were tested against 149 sera from patients with NCC confirmed by brain imaging, 40 sera from patients with other parasitic diseases, and 131 sera from US. individuals without evidence of neurocysticercosis (clinical/serological/brain imaging). The sensitivity and specificity of each antigen by WB were calculated by counting the number of true positive, false positive, true negative and false negative results. Using the receiver operating characteristic (ROC) curves, the cut-off values for the ELISA and MBA were established as well as the sensitivity and specificity of each assay. All three antigens showed a high sensitivity on WB in active NCC cases with two or more viable cysts and low sensitivity for cases with single viable cyst or calcified lesions and inactive NCC. WB showed the highest specificity and sensitivity out of the three diagnostic techniques. The recombinant T24H-his was the best diagnostic reagent in WB (100% sensitivity, 99.4% specificity), exhibiting similar results to the LLGP-EITB, against the same panel of NCC sera. The GST-T24H antigen worked better than the others in ELISA and MBA protocols (88.3 and 96.1% sensitivity, respectively and 96.5% specificity). The sensitivity and specificity that we obtained were similar to results from a previous study using a similar recombinant antigen (rT24H), suggesting that recombinant antigens may be good alternatives to crude extracts in a variety of diagnostic techniques. Furthermore, these antigens can be applied in the development of point-of-care tests which would be useful in NCC field studies.

Neurocysticercosis

Taenia solium

Multiplex

10.1186/s13071-017-2160-2

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Citations (24)

Taeniasis and Cysticercosis

Springer eBooks (2022)

Elizabeth Ferrer María Jesús Perteguer

Taeniasis

Taenia solium

Neurocysticercosis

Taenia

Parasitology

Neglected Tropical Diseases

10.1007/978-3-031-00303-5_9

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Citations (5)

Aplicación de la técnica PCR-RFLP en la identificación de nematodos implicados en la anisakiasis humana

Medicina Clínica (2004)

María Jesús Perteguer Guadalupe Ortiz Elena García García María Flores‐Chávez Esperanza Rodríguez

10.1016/s0025-7753(04)74356-0

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Citations (1)

Comparison of recombinant cathepsins L1, L2, and L5 as ELISA targets for serodiagnosis of bovine and ovine fascioliasis

Parasitology Research (2018)

Victoria Martínez‐Sernández María Jesús Perteguer Ana Hernández-González Mercedes Mezo Marta González–Warleta

Hepatica

Fasciolosis

10.1007/s00436-018-5809-7

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Citations (18)

Enzyme-linked immunosorbent assay, immunoblot analysis and RAST fluoroimmunoassay analysis of serum responses against crude larval antigens of Anisakis simplex in a Spanish random population

Journal of Helminthology (1996)

L. García-Palacios Marta González Micaela Esteban E. Mirabent María Jesús Perteguer

The results obtained in a study of seroprevalence by means of ELISA and immunoblot with crude larval extracts of Anisakis simplex using 1008 human sera from Spanish people showing no clinical suspicion of anisakidosis are given. For the evaluation of the results obtained by ELISA the Diagnostic Index (DI) was used, as the ratio between the optical density resulting from the test serum and the optical density of the negative control. Forty-seven sera showed DIs between 1.5 and 2, and 14 sera were greater than 2. After comparison of the immunoblot analysis with the immunorecognition pattern of a human anisakidosis reference serum, a diagnostic criterion could be established for those sera that, at a 1/100 dilution, showed a DI by ELISA greater than 1.5. Seven of 14 selected sera with DIs in ELISA higher than 1.3 showed anti-Anisakis specific IgE antibodies by RAST fluoroimmunoassay.

Anisakis simplex

Seroprevalence

10.1017/s0022149x0001556x

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Citations (33)

Transgenesis in parasitic helminths: a brief history and prospects for the future

Parasites & Vectors (2022)

Monica J. Quinzo María Jesús Perteguer Paul J. Brindley Alex Loukas Javier Sotillo

Helminth infections impact the health of hundreds of millions of persons globally and also cause important economic losses in livestock farming. Methodological limitations as well as the low attention given to the study of helminths have impacted biological research and, thus, the procurement of accurate diagnosis and effective treatments. Understanding the biology of helminths using genomic and proteomic approaches could contribute to advances in understanding host-helminth interactions and lead to new vaccines, drugs and diagnostics. Despite the significant advances in genomics in the last decade, the lack of methodological adaptation of current transgenesis techniques has hampered the progression of post-genomic research in helminthology. However, the application of new techniques, such as CRISPR, to the study of trematodes and nematodes has opened new avenues for genome editing-powered functional genomics for these pathogens. This review summarises the historical advances in functional genomics in parasitic helminths and highlights pending limitations that will need to be overcome to deploy transgenesis tools.

Transgenesis

Functional Genomics

10.1186/s13071-022-05211-z

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Citations (27)

Antibody responses to chimeric peptides derived from parasite antigens in mice and other animal species

Molecular Immunology (2018)

Ricardo A. Orbegozo-Medina Victoria Martínez‐Sernández I. Folgueira Mercedes Mezo Marta González–Warleta

Chimera (genetics)

10.1016/j.molimm.2018.11.019

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Citations (1)