Introduction: The effect of in vitro fertilization (IVF) on neurodevelopmental outcome is unclear. Probably, IVF does not affect traditional measures of neurological development in infancy, but little is known on long-term effects. Recently a new video-based instrument to evaluate neuromotor development was developed. The Infant Motor Profile (IMP) evaluates neuromotor condition in infants in terms of the quality of spontaneous motor behaviour. The IMP does not only assess traditional neuromotor domains, such as performance, symmetry and movement fluency, but also two novel domains: variation and variability. Movement variation denotes the size of the child's movement repertoire. Previous studies demonstrated that reduced movement variation is associated with early lesions of the periventricular white matter and - more generally - reflects the integrety of cortical connectivity. In addition, reduced variation during infancy is associated with neurodevelopmental disorders in later life and reduced intelligence at school age. Variability denotes the child's ability to select from the repertoire of movements the strategy that suits the situation best. In this prospective, assessor-blinded cohort study, we address the question whether ovarian hyperstimulation and/or the in vitro procedure affect movement variation during infancy. Material and Methods: Singletons born following IVF with conventional controlled ovarian hyperstimulation (COH-IVF, n = 68), following IVF in a modified natural cycle (MNC-IVF, n = 57) and following natural conception born to subfertile couples (Sub-NC, n = 90) were assessed with the IMP at 4, 10 and 18 months. The assessment resulted in a total IMP score and 5 domain scores: variation (i.e. the size of movement repertoire), variability (i.e. the ability to select motor strategies), symmetry, fluency and performance. Primary outcome was the domain score variation. Outcome variables were analyzed with a mixed effects model. Results: The mixed model indicated an overall effect of treatment on variation (p Conclusions: The preliminary data suggest that ovarian hyperstimulation may be associated with reduced movement variation in infancy. These findings stress the need to carefully monitor neurodevelopment in IVF offspring.
Abstract Depending on management, cover crops may improve soil and environmental quality and tomato ( Solanum lycopersicum L.) yield. We evaluated the effects of hairy vetch (HV; Vicia villosa R.) residue management and the biculture of HV and rye ( Secale cereale L.) on soil organic carbon (SOC) and total nitrogen (STN), microbial biomass nitrogen (MBN), soil inorganic nitrogen, and tomato yield for 2 yr in a plastic high tunnel. The SOC in the surface 10‐cm depth was 2.87–17.5% significantly greater in HV incorporation (HVI), HV mulch (HVM), and the biculture of HV and rye treatments (HV+RYE), than in a no cover crop treatment (bare fallow). However, cover crop management effects on STN varied with soil depths (0‐ to 10‐ and 10‐ to 30‐cm depths) and years, and HVI tended to be more effective than other treatments in increasing STN. Residual soil nitrate−N was increased by cover crops, more so by HV monoculture than HV+RYE. The MBN and inorganic N (NO 3 − −N + NH 4 + −N) were greater in HVI than either HVM or HV+RYE. Tomato total yield was 11.1–43.8% significantly greater in HVI and HVM than in bare fallow. However, the effects of HV+RYE on MBN, inorganic N, and tomato yield varied with C/N ratio of residues and best results were obtained with a C/N ratio of 17.6 than with 23.7. Therefore, if an adequate seeding HV/rye ratio (2:1) is used, HV+RYE is a better management practice to increase SOC and STN at topsoil and tomato yield with least residual N.
Linkage tests were conducted between an esterase locus, Est1, and loci coding for four isozymes and two morphological markers in a cross between cultivars Shiratsurunoko and Suzuhime. The loci tested were Dia1 (diaphorase), Enp (endopeptidase), Idh2 (isocitrate dehydrogenase), Pgm1 (phosphoglucomutase), Ln (leaf shape), and W1 (flower or hypocotyl color). A contingency chi-square test indicated a significant deviation from the expectation of independent inheritance between the Est1 and the Enp and Ln loci, members of linkage group 4. Based on the combined F2 and F3 data, recombination values were estimated as 32.3 ± 2.1% for Est1 versus Enp and 20.4 ± 2.2% for Est1 versus Ln. The recombination value between the Enp and Ln loci was calculated as 15.8 ± 2.0%. The Est1 locus was therefore assigned to linkage group 4, and the gene order was determined as Est1-Ln-Enp.
In order to improve the use efficiency of a cover crop, hairy vetch (Vicia villosa R., HV), and supplemental chemical N fertilizer, N release and uptake patterns from HV, fast-release N fertilizer (Fast), and slow-release N fertilizer (Slow) in fresh market tomato (Solanum lycopersicum L.) production were investigated using the 15N-labeling method. In the incubation of soil-added N at two mix rates, 20% Fast + 80% Slow (FS) and 100% Slow (S), a large amount of inorganic N, mainly NH4+-N, was released by FS in 4 weeks. Tomato ‘House momotaro’ was grown in 1/2000 a Wagner pots incorporating such N fertilizer and 15N-labeled HV residue (30 g DW/pot, about 200 kg N·ha−1). Plant biomass in tomato grown with HV was larger than that grown without HV. HV-derived N (Ndfhv) was taken up by the tomatoes mainly until 4 weeks after transplant (WAT). The uptake amount of Ndfhv was the same in the pot with HV-FS and HV-S. The rate of N uptake derived from HV to total N uptake in tomato plants (%Ndfhv) was 43% in HV-S, higher than that in HV-FS (34%) in 4 WAT; however, such a difference disappeared after 4 WAT. N uptake by tomato plants continued until 12 WAT. Based on these results, HV acted as a fast-release fertilizer. There was competition in N uptake between chemical fertilizer N and HV-released N in the early stage of tomato cultivation. A large amount of chemical fertilizer tended to suppress the uptake of Ndfhv. N uptake by tomato plant continued until the late stage. These results can be applied to establish a suitable combination of HV and chemical fertilizer for tomato production.
One of the ways to reduce chemical fertilizer application is the use of cover crops, which improve soil properties and supply nutrition to subsequent crops. The application effect of a legume cover crop, hairy vetch (Vicia villosa R., HV), on N dynamics in fresh market tomatoes (Solanum lycopersicum L.), 'House Momotaro,' was investigated using the 15N-labeling method. Tomato seedlings were transplanted into a 1/2000 a Wagner pot at 0, 80, and 240 kg·ha−1 of N application (N0HV, N80HV, and N240HV) on June 9, 2011. Before transplanting, the labeled HV and chemical fertilizers were incorporated into the soil. Five tomato plants were collected 6 times in each treatment and then separated into leaves, stems, and roots. Fruits were harvested at maturity. HV-derived N uptake was recognized mainly in the first 4 weeks after transplant (WAT). Especially in N240HV, HV-derived N uptake ceased at 4 WAT. The uptake amounts of HV-derived N at 10 WAT were 587, 657, and 729 mg·plant−1 in N240HV, N80HV, and N0HV, respectively, and were increased by decreasing N fertilizer application. The rate of N uptake derived from HV to total N uptake in tomato plants (%Ndfhv) was the highest at 2 WAT, and %Ndfhv in N80HV (52.1%) and N0HV (51.5%) were significantly higher than in N240HV (43.6%). After 2 WAT, %Ndfhv decreased gradually in all N rates as tomatoes grew and decreased to 24.8%, 34.4%, and 37.1% in N240HV, N80HV, and N0HV, respectively, until 12 WAT. Nitrogen use efficiency (NUE) from HV-derived N was the highest at 10 WAT, and N0HV (55.3%) was significantly higher than N240HV (44.5%) and N80HV (49.8%). The partition rate of HV-derived N into fruits was 63.9%, and 39.7% of HV-derived N was distributed into 1st and 2nd fruit clusters. From these results, it was clarified that HV can be expected to be an alternative N fertilizer because HV-derived N was absorbed effectively with a small amount of N fertilizer. Further research on fertilizer management in tomato's early stage will be needed for an N-reduction system because HV-derived N was mainly absorbed for 4 WAT.