We describe a 60-year-old woman with leg pain. Although metastatic bone tumor and atypical cells mimicking signet-ring cells in the bone marrow picture were observed, systemic survey revealed no primary lesion. The patient died two months after admission from systemic progress of the disease. Autopsy revealed a small focus of adenocarcinoma within the right upper lobe of the lung and systemic metastases without any particular changes in the gastrointestinal tract. The tumor cells of the lung were diffusely positive for cytokeratin 7, whereas cytokeratin 20 immunoreactivity was weak and focal, and that supported the lung origin of the present tumor. Moreover, the tumor cells in the bone marrow showed a similar pattern in immunoreactivity. These findings suggest that cytokeratin 7 and cytokeratin 20 immunoreactivity is helpful for the premorten diagnosis of the metastatic tumor of unknown origin.(Internal Medicine 37: 766-769, 1998)
Abstract Previous investigations have demonstrated that CTL may play an important role in suppressing the disease progression of HIV infection. In this study, we inoculated mice with IL-12 expression plasmid together with plasmid-encoding HIV-1 envelope to enhance CTL activity by activating a Th1-type response. The results of delayed-type hypersensitivity using the footpad swelling response and of CTL activity clearly showed that HIV-1-specific cell-mediated immunity was enhanced by inoculation of the IL-12 expression plasmid. Quantitation of cytokine in the sera of IL-12-inoculated mice revealed that IFN-gamma significantly increased. The enhanced cell-mediated immunity responses were abrogated by combined administration of the IL-12 expression plasmid and neutralizing anti-IFN-gamma Ab. Together, these results suggest that enhanced virus-specific cell-mediated immunity occurred via an endogenously produced IFN-gamma by inoculation of IL-12 expression plasmid.
The interaction between oral non-steroidal anti-inflammatory drugs (NSAIDs) and prednisolone administered concurrently for 30 days was studied in 18 healthy dogs divided into 3 groups of 6 dogs each: a drug-free negative control group (NC group) given 2 gelatin capsules; a group given meloxicam (0.1 mg/kg) and prednisolone (0.5 mg/kg) (MP group); and a group given a reduced dosage of ketoprofen (0.25 mg/kg, PO) and prednisolone (0.5 mg/kg, PO) (KP group). The dogs were periodically monitored by physical examinations, blood analyses, endoscopic examinations, fecal occult blood tests, renal function tests [effective renal plasma flow (ERPF) and glomerular filtration rate (GFR)], urinalyses [urinary sediments, and urinary micro-albumin to creatinine ratio (UAlb/Cre)], urinary enzyme indices, and haemostatic function tests [buccal mucosa bleeding time (BMBT), cuticle bleeding time (CBT)]. Significant changes were observed in the KP group, including a decrease of ERPF and GFR, an increased UAlb/Cre ratio, prolonged BMBT and CBT, as well as the presence of more severe grades of endoscopic lesions and fecal occult blood. In both the MP and KP groups, abnormal enzymuria with exfoliation of renal tubular epithelial cells in the urine was found. However, no significant changes in any of the other tests were observed in the MP group compared with the NC group. These findings suggest that the combination of NSAIDs, even selective COX-2 inhibitors, with prednisolone may be contraindicated due to the potential for serious adverse effects on the kidneys, the platelets, and the gastrointestinal tract.
The purpOse of this study was to investigate the effect of voluntary running exercise o■ serun triacylglycerol(TG)concentratio■ in male rats. Rats were divided into two grOups in age of 9 wk in eXperiment l and in age of 6wk in experiment 2. 0■ o grOup Of rats was maintained sendentary and a second group of rats was allowed Voluntary wheel Funning during darkneSS periOd. Both groups of rats were housed individual17 on a 12hF dark‐ light cycle and llleal― fed twice a dav. TG remoヤ al ratO wa‐s measttred on rats at the end of 3wk feeding period in experilllent l and at the end of 5wk feeding period in experinent 2. TG secretion rate was measured on rats in one week after the llleasurement of TC re■ oval rate. Results weire as follows. 1. Voluntary running activity of exercised rats averaged 3km/day in experiment l and ・ 10km/day in experilllent 2. 2。 Weight of adiloふ e tissue was lighter in exercised rats than in sedentary rats in both、 experi■ enむs。 ・ 3。 Serum TG level and TC rellloval rate weFe nOt Significantiv different between sedentary rats and eXercised rats in both experilllents. 4. Hepatic― intestinal TC secretion rate Was ■ot Significantly different between two grOups in eXperiment l, but was significantly lower in exercised rats than in sed― entary rats in experiment 2. These FeSults suggest that the voluntary running activitv lllav inhibit the ac‐ oulllurat io■ of adipose tissue and depress the hepatic-1■ testinal TG secretion rate in youn‐ g active rats. 緒 言 血申Triacylglycerol(TG)濃 度は,肝臓及び小腸から血申へのVLDL=TCおよびカイロミクロ
A 18-year old female developed polyarthralgia and Raynaud's phenomenon in 1973. She was admitted to the hospital because of recurrent fever in 1976, and diagnosed as having SLE, manifested by facial butterfly rash, nonerosive arthritis, proteinuria, leukopenia, positive LE cell preparation, abnormal titer of antinuclear antibody and anti-DNA antibody.The patient had episodes of herpes zoster at 4, 28, 38, 52 and 59 months after initial symptoms of SLE. The course of herpes zoster was favorable and all episodes resolved without complications.She had deficiency in cell-mediated immunity, such as skin tests with PPD, DNCB, and IgG Fc receptor positive T cells. But the abnormalities were found both with and without herpes zoster infection. Decrease of complement titer was observed whenever herpes zoster occurred.She first suffered from herpes zoster prior to the initiation of steroid theroid therapy, and received prednisolone at doses from 12.5mg to 30mg daily on 4 episodes of herpes zoster.It seemed that one of the causes on the multiple zoster infections, which were observed in early time after the onset of SLE, was low level of serum complement.
To clarify the clinical significance of autoantibodies to interleukin-1 alpha (IL-1 alpha autoantibodies) in rapidly progressive idiopathic pulmonary fibrosis (IPF), we measured the level of IL-1 alpha autoantibodies in serum of 11 patients on the first hospital day, when patients were admitted due to severe symptoms, and on the 21st hospital day. IL-1 alpha autoantibodies in serum were measured using radioimmunoassay, and the limitation of this assay for IL-1 alpha autoantibodies was 5 ng/ml. These antibodies were detected in 5 of 11 patients on the first hospital day. On the 21st hospital day, these antibodies were detected in all patients, and its level was increased compared with that on the first hospital day. IL-1 alpha autoantibodies that appeared in patients corresponded to that of IgG. The half life of exogenous autoantibodies was investigated following administration of autoantibody rich plasma obtained from healthy blood donors to 6 control patients (CP) and 6 progressive IPF patients. These autoantibody levels in their serum were less than 5 ng/ml before administration. Serum was obtained at the indicated time after administration of IL-1 alpha autoantibodies and the level of these autoantibodies in serum was measured, then the half life was calculated. Half life of exogenous IL-1 alpha autoantibodies in progressive IPF patients was significantly shorter than that in CP (71.3 +/- 31.8 hr vs 352.0 +/- 98.3 hr, p < 0.01). These findings suggested that IL-1 alpha autoantibodies were generated in response to the inflammatory process of rapidly progressive IPF and may act as a regulatory factor for IL-1 alpha.
