Fattah Sotoodehnejadnematalahi

Islamic Azad University, Science and Research Branch

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Abolfazl Fateh

Pasteur Institute of Iran

Seyed Davar Siadat

Pasteur Institute of Iran

Enayat Anvari

Ilam University

Fatemeh Sakhaee

Pasteur Institute of Iran

Mohammad Saber Zamani

Shahed University

B. F. Burke

Coventry University

Hossein Baharvand

University of Science and Culture

Akram Eidi

Islamic Azad University, Science and Research Branch

Sirous Zeinali

Pasteur Institute of Iran

Masoumeh Azizi

Pasteur Institute of Iran

Cooperative Institutions

Islamic Azad University, Science and Research Branch

Tehran University of Medical Sciences

Academic Center for Education, Culture and Research

Royan Institute

Shahid Beheshti University of Medical Sciences

Pasteur Institute of Iran

Iran University of Medical Sciences

University of Tehran

Tarbiat Modares University

Islamic Azad University, Tehran

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Recombinant expression of SARS-CoV-2 receptor binding domain (RBD) in Escherichia coli and its immunogenicity in mice.

DOAJ (DOAJ: Directory of Open Access Journals) (2022)

Zahra Rahbar Shahram Nazarian Ruhollah Dorostkar Fattah Sotoodehnejadnematalahi Jafar Amani

The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), giving rise to the coronavirus disease 2019 (COVID-19), has become a danger to wellbeing worldwide. Thus, finding efficient and safe vaccines for COVID-19 is of great importance. As a basic step amid contamination, SARS-CoV-2 employs the receptor-binding domain (RBD) of the spike protein to lock in with the receptor angiotensin-converting enzyme 2 (ACE2) on host cells. SARS-CoV-2 receptor-binding domain (RBD) is the main human antibody target for developing vaccines and virus inhibitors, as well as neutralizing antibodies. A bacterial procedure was developed for the expression and purification of the SARS-CoV-2 spike protein receptor-binding domain.In this research study, RBD was expressed by Escherichia coli and purified with Ni-NTA chromatography. Then it was affirmed by the western blot test. The immunogenicity and protective efficacy of RBD recombinant protein were assessed on BALB/c mice. Additionally, RBD recombinant protein was tested by ELISA utilizing sera of COVID-19 healing patients contaminated with SARS-CoV-2 wild type and Delta variation.Indirect ELISA was able to detect the protein RBD in serum of the immunized mouse expressed in E. coli. The inactive SARS-CoV2 was detected by antibodies within the serum of immunized mice. Serum antibodies from individuals recovered from Covid19 reacted to the expressed protein.Our findings showed that RBD is of great importance in vaccine design and it can be used to develop recombinant vaccines through induction of antibodies against RBD.

Coronavirus

10.22038/ijbms.2022.65045.14333

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Citations (8)

rs12329760 Polymorphism in Transmembrane Serine Protease 2 Gene and Risk of Coronavirus Disease 2019 Mortality

BioMed Research International (2022)

Saeedeh Sadat Beheshti Shirazi Fatemeh Sakhaee Fattah Sotoodehnejadnematalahi Mohammad Saber Zamani Iraj Ahmadi

The protease produced by the transmembrane serine protease 2 (TMPRSS2) gene enhances viral infections and has been linked to severe acute respiratory syndrome coronavirus 2 pathogenesis. Therefore, this study evaluated the association between TMPRSS2 and coronavirus disease 2019 (COVID-19) mortality. TMPRSS2 rs12329760 polymorphism was genotyped using the tetraprimer amplification refractory mutation system-polymerase chain reaction method in 592 dead and 693 improved patients. In the current study, the frequency of TMPRSS2 rs12329760 CC than TT genotypes was significantly lower in improved patients than in dead patients. According to the findings of the multivariate logistic regression test, higher levels of mean age, creatinine, erythrocyte sedimentation rate, C-reactive protein, aspartate aminotransferase, lower levels of 25-hydroxyvitamin D, uric acid, and real-time PCR Ct values and TMPRSS2 rs12329760 CC genotype were observed to be associated with increased COVID-19 mortality rates. In conclusion, the TMPRSS2 rs12329760 CC genotype was a polymorphism linked to a significantly higher incidence of severe COVID-19. Further studies are required to corroborate the obtained findings.

TMPRSS2

Coronavirus

Gene polymorphism

10.1155/2022/7841969

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Citations (9)

Prokaryotic expression, evaluation, and prediction of the structure and function of the ecarin metalloproteinase domain

Proteins Structure Function and Bioinformatics (2021)

Nasrin Mohammadi Mojgan Bandehpour Fattah Sotoodehnejadnematalahi Bahram Kazemi

Ecarin is one of the most widely used drug compounds in blood clotting experiments and is used to monitor and treat many diseases such as cancer, liver, lupus, and cardiovascular disease. The metalloproteinase domain is known as the active site of ecarin. In this study, an ecarin metalloproteinase cassette was designed and synthesized in the pUC57 vector. The gene fragment was released and cloned into the pET-28a vector and expressed in Escherichia coli. The recombinant protein was confirmed by western blotting. Enzyme activity was estimated by a laboratory coagulation test, and prothrombin time and tertiary structure were determined by using the Iterative Threading ASSEmbly Refinement (I-TASSER) server. Data from blood clotting tests for the produced ecarin activity were analyzed using an independent t test. As per I-TASSER server prediction, model 1 with the highest confidence score 0.95, template modeling score (0.84 ± 0.08), and root mean square deviation (3.5 ± 2.4 Å) was considered as the best model, and the 2e3xA enzyme was more similar to the target protein. The predictive results helped to better understand the relationship between the structure and function of the ecarin metalloproteinase domain. Also, the production of this active site in the prokaryotic expression system, which is simpler and more cost-effective than the production of the eukaryotic system, showed that this recombinant ecarin could be used as a substitute for the raw snake venom of Echis carinatus because it converts prothrombin into thrombin, and its activity, as estimated using the prothrombin time test, was found to be faster than normal ecarin.

Prothrombin time

10.1002/prot.26275

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Citations (2)

Increased risk of COVID-19 mortality rate in IFITM3 rs6598045 G allele carriers infected by SARS-CoV-2 delta variant

Human Genomics (2022)

Melika Gholami Fatemeh Sakhaee Fattah Sotoodehnejadnematalahi Mohammad Saber Zamani Iraj Ahmadi

The interferon-induced transmembrane-protein 3 (IFITM3) is a vital component of the immune system's defense against viral infection. Variants in the IFITM3 gene have been linked to changes in expression and the risk of severe Coronavirus disease 2019 (COVID-19). This study aimed to investigate whether IFITM3 rs6598045, quantitative polymerase chain reaction (qPCR) cycle threshold (Ct) values, and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants are associated with an increased mortality rate of COVID-19.The genotyping of IFITM3 rs6598045 polymorphism was analyzed using the amplification refractory mutation system-polymerase chain reaction in 1342 recovered and 1149 deceased patients positive for SARS-CoV-2.In this study, IFITM3 rs6598045 G allele as minor allele frequency was significantly more common in the deceased patients than in the recovered ones. Furthermore, the highest mortality rates were observed in Delta variant and lowest qPCR Ct values. COVID-19 mortality was associated with IFITM3 rs6598045 GG and AG in Delta variant and IFITM3 rs6598045 AG in Alpha variant. A statistically significant difference was observed in the qPCR Ct values between individuals with GG and AG genotypes and those with an AA genotype.A possible correlation was observed between the mortality rate of COVID-19, the G allele of IFITM3 rs6598045, and SARS-CoV-2 variants. However, large-scale research is still required to validate our results.

Coronavirus

10.1186/s40246-022-00434-8

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Citations (17)

Platinum Nanoparticles as Potent Anticancer and Antimicrobial Agent: Green Synthesis, Physical Characterization, and In-Vitro Biological Activity

Journal of Cluster Science (2022)

Mohammadhassan Gholami‐Shabani Fattah Sotoodehnejadnematalahi Masoomeh Shams‐Ghahfarokhi Ali Eslamifar Mehdi Razzaghi‐Abyaneh

Platinum nanoparticles

Zeta potential

Nanochemistry

Nanomaterials

10.1007/s10876-022-02225-6

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Citations (19)

In-silico Analysis of Pneumococcal Heat-Shock Protein (DnaJ) to Predict Novel Multi-Epitope Vaccine Candidates

Vaccine Research (2021)

Elnaz Afshari Reza Ahangari Cohan Fattah Sotoodehnejadnematalahi Seyed Fazlollah Mousavi

In-silico Analysis of Pneumococcal Heat-Shock Protein (DnaJ) to Predict Novel Multi-Epitope Vaccine Candidates

Epitope mapping

10.52547/vacres.8.2.65

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Citations (2)

The Effect of Akkermansia muciniphila and Its Outer Membrane Vesicles on MicroRNAs Expression of Inflammatory and Anti-inflammatory Pathways in Human Dendritic Cells

Probiotics and Antimicrobial Proteins (2023)

Laya Zoghi Mofrad Abolfazl Fateh Fattah Sotoodehnejadnematalahi Dariush Norouzian Sham Asbi Seyed Davar Siadat

Akkermansia muciniphila

CD80

10.1007/s12602-023-10058-6

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Citations (5)

Angiotensin‐converting enzyme 2 rs2285666 polymorphism and clinical parameters as the determinants of COVID‐19 severity in Iranian population

International Journal of Immunogenetics (2022)

Fereshteh Khalilzadeh Fatemeh Sakhaee Fattah Sotoodehnejadnematalahi Mohammad Saber Zamani Iraj Ahmadi

Host genetic factors may be correlated with the severity of coronavirus disease 2019 (COVID-19). Angiotensin-converting enzyme 2 (ACE2) plays a vital role in viral cell entrance. The current study aimed to evaluate the association of ACE2 rs2285666 polymorphism and clinical parameters with COVID-19 mortality. The ACE2 rs2285666 polymorphism was genotyped using the polymerase chain reaction-restriction fragment length polymorphism in 556 recovered and 522 dead patients. In this study, the frequency of ACE2 rs2285666 CC was significantly higher than TT genotype in dead patients. The multivariate logistic regression analysis results showed that the higher levels of alanine aminotransferase, alkaline phosphatase, creatinine, erythrocyte sedimentation rate, and C-reactive protein and the low levels of uric acid, cholesterol, low density lipoprotein, 25-hydroxyvitamin D, real-time PCR Ct values, and ACE2 rs2285666 CC genotype were associated with increased mortality rates after COVID-19. In conclusion, our findings demonstrated a possible link between COVID-19 mortality, clinical parameters, and ACE2 rs2285666 CC. Further research is required to confirm these results.

Angiotensin-converting enzyme 2

10.1111/iji.12598

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Citations (25)

The Effect of Carvacrol on the Expression of Genes Hmox-1, iNOS, Nrf2 and NF-ҚB in the Spinal Cord of Experimental Autoimmune Encephalomyelitis Mice

Iranian South Medical Journal (2022)

Mahdeiah Ahmadi Akram Eidi Hassan Ahmadvand Mojtaba Khaksarian Fattah Sotoodehnejadnematalahi

The Effect of Carvacrol on the Expression of Genes Hmox-1, iNOS, Nrf2 and NF-ҚB in the Spinal Cord of Experimental Autoimmune Encephalomyelitis Mice

Carvacrol

Encephalomyelitis

10.52547/ismj.25.1.1

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