To assess the use of high-resolution melting(HRM) analysis for rapid identification of porcine circovirus-like agent P1 and porcine circovirus type 2(PCV2),the targeted DNA sequence was amplified by Corbett Rotor-Gene 6000 real-time PCR cycler in the presence of the EvaGreen fluorescent dye.A working protocol of HRM analysis were established.Under the optimized assay conditions,P1 and PCV2 were amplified and two HRM types were differed by only 1 bp.This assay provides a new approach for P1 and PCV2 detection using a new molecular diagnostic method.
Abstract A series of ZnGa 2 O 4 :Tb 3+ ,Ce 3+ persistent luminescence nanophosphors were prepared by high temperature solid‐state reaction. The X‐ray diffraction (XRD) patterns of the representative samples are consistent with that of the ZnGa 2 O 4 standard card. Scanning electron microscope (SEM) images show that the particle size of phosphor is enlarged when the Tb 3+ ion is doped, however, it becomes smaller again after the Ce 3+ ion is codoped. For ZnGa 2 O 4 :Tb 3+ nanophosphors, there exist intense 492 and 551 nm blue/green emissions under 280 nm excitation, and the optimum Tb 3+ doping concentration is 1.7 mol% and the long afterglow time is about 140 min. The PL and long afterglow characteristics of ZnGa 2 O 4 :1.7Tb 3+ nanophosphor are significantly improved once the Ce 3+ ion is codoped. The PL intensity of ZnGa 2 O 4 :1.7Tb 3+ nanophosphor is enhanced by 1.5 times and the long afterglow time is extended to 210 min when Ce 3+ doping concentrations are 0.3 and 0.5 mol%, respectively. The internal mechanism of the long afterglow effect of ZnGa 2 O 4 :1.7Tb 3+ ,0.5Ce 3+ nanophosphor is discussed in detail with the help of measurement results of thermoluminescence spectra.
To explore microRNA (miR)-193b expression and its potential role in colon cancer, reverse transcription-quantitative polymerase chain reaction was performed to detect the miR-193b expression levels in 62 colon cancer tissues and normal adjacent tissues. The miR-193b-overexpressed cell line SW620 was used to study the role of miR-193b in colon cancer. Subsequently, a Transwell assay and cell cycle assay were performed to observe the functional cell changes in the in vitro expression levels of miR-193b. Results indicated that miR-193b expression levels were significantly decreased in colon cancer tissues compared with adjacent normal tissue (P<0.001) and the expression of miR-193b was significantly correlated with TNM staging (P=0.03) and lymph node invasion (P=0.007). Furthermore, overexpression of miR-193b significantly decreased colon cancer cell cycle progression and its migration ability. In addition, the present findings suggested that the increased expression of miR-193b by RAB22A, inhibited downstream proteins involved in the Ras signaling pathway, including the Ras and extracellular signal-related kinase which may inhibit cancer proliferation and migration. In conclusion, the aim was to clarify the association of miR-193b expression with colon cancer, and to explore the mechanism of miR-193b in colon cancer proliferation and cell migration. The preliminary findings revealed that miR-193b may have an important role in the process in colon cancer cell cycle and migration by the RAB22A-Ras signaling pathway, thus providing a theoretical basis for miR-193b as a potential molecular target for colon cancer treatment.
The relationship between the net gain of two kinds of glass with high dopant concentrations and pumping power,thread lengths is measured at room temperature.The results show that,under 100mW pumping power,the net gain of Er-doped glass thread with 0.19g/cm~3 concentration is 1.96dB/cm,its threshold pumping power is 36mW and optimum length is 4.5cm.The net gain of Yb∶Er-codoped glass thread with 1.1g/cm~3 Yb-concentration and 0.12g/cm~3 Er-concentration is 3.07dB/cm,its threshold pumping power is 28mW and optimum length is 2.5cm.That is,10dB total net gain can be obtained within 10cm length.The net gain of Yb∶Er-codoped glass threads don't take on the trend of saturation when pumping power becomes stronger.It means that more sensitive ytterbium improves the net gain characteristics of the Er-doped fiber amplifiers.
The expression of nitric oxide synthase (NOS_(1)) was observed in the normal brain and spinal cord or macaque monkey with immunohistochemical method. NOS_(1)-positive neurons were distributed in extensive regions of CNS, including cerebral cortex, pyramidal layer of hippocampus, granular layer of dentate gyrus, caudate-putamen, corpus striatum, Purkinje^s cells, cerebeller, cerebellar cortex, anterior horn, central grey and posterior horn of spinal cord. The extensive distribution of NOS_(1) indicates that NO is involved in many functions of CNS, such as learning and memory.
ABSTRACT Oligodendrocyte precursor cells (OPCs) undergo a series of energy-consuming developmental events; however, the uptake and trafficking pathways for their energy metabolites remain unknown. In the present study, we found that 2-NBDG, a fluorescent glucose analog, can be delivered between astrocytes and oligodendrocytes through connexin-based gap junction channels but cannot be transferred between astrocytes and OPCs. Instead, connexin hemichannel-mediated glucose uptake supports OPC proliferation, and ethidium bromide uptake or increase of 2-NBDG uptake rate is correlated with intracellular Ca2+ elevation in OPCs, indicating a Ca2+-dependent activation of connexin hemichannels. Interestingly, deletion of connexin 43 (Cx43, also known as GJA1) in astrocytes inhibits OPC proliferation by decreasing matrix glucose levels without impacting on OPC hemichannel properties, a process that also occurs in corpus callosum from acute brain slices. Thus, dual functions of connexin-based channels contribute to glucose supply in oligodendroglial lineage, which might pave a new way for energy-metabolism-directed oligodendroglial-targeted therapies.
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