Gary L. Glish

University of North Carolina at Chapel Hill

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Scott A. McLuckey

Purdue University West Lafayette

Gary J. Van Berkel

Oak Ridge National Laboratory

Paul M. Armistead

University of North Carolina Health Care

Sally A. Hunsucker

Segeberger Kliniken

Gheath Alatrash

The University of Texas MD Anderson Cancer Center

Atim A. Enyenihi

University of North Carolina at Chapel Hill

Tania Rodríguez-Cruz

Houston Methodist

Gregory Lizée

The University of Texas MD Anderson Cancer Center

Patricia A. Ropp

University of North Carolina at Chapel Hill

R. Graham Cooks

Purdue University West Lafayette

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University of North Carolina at Chapel Hill

141

Oak Ridge National Laboratory

University of North Carolina Health Care

UNC Lineberger Comprehensive Cancer Center

Communities In Schools of Orange County

Lung Institute

The University of Texas MD Anderson Cancer Center

Purdue University West Lafayette

Indiana University School of Medicine

Segeberger Kliniken

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Collisional cooling in a quadrupole ion trap at sub-ambient temperatures

International Journal of Mass Spectrometry (2007)

Philip M. Remes Gary L. Glish

Infrared multiphoton dissociation

Quadrupole ion trap

Ion trapping

10.1016/j.ijms.2007.02.004

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Citations (17)

Collision-activated dissociation of negative ions in an ion trap mass spectrometer

Analytical Chemistry (1987)

Scott A. McLuckey Gary L. Glish Paul E. Kelley

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCollision-activated dissociation of negative ions in an ion trap mass spectrometerScott A. McLuckey, Gary L. Glish, and Paul E. KelleyCite this: Anal. Chem. 1987, 59, 13, 1670–1674Publication Date (Print):July 1, 1987Publication History Published online1 May 2002Published inissue 1 July 1987https://doi.org/10.1021/ac00140a019RIGHTS & PERMISSIONSArticle Views207Altmetric-Citations67LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (661 KB) Get e-Alerts Get e-Alerts

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Trap (plumbing)

10.1021/ac00140a019

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Citations (68)

The basics of mass spectrometry in the twenty-first century

Nature Reviews Drug Discovery (2003)

Gary L. Glish Richard W. Vachet

Identification

10.1038/nrd1011

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Citations (419)

Evaluation of e-liquid toxicity using an open-source high-throughput screening assay

PLoS Biology (2018)

Maria F. Sassano Eric S. Davis James E. Keating Bryan Zorn Tavleen K. Kochar

The e-liquids used in electronic cigarettes (E-cigs) consist of propylene glycol (PG), vegetable glycerin (VG), nicotine, and chemical additives for flavoring. There are currently over 7,700 e-liquid flavors available, and while some have been tested for toxicity in the laboratory, most have not. Here, we developed a 3-phase, 384-well, plate-based, high-throughput screening (HTS) assay to rapidly triage and validate the toxicity of multiple e-liquids. Our data demonstrated that the PG/VG vehicle adversely affected cell viability and that a large number of e-liquids were more toxic than PG/VG. We also performed gas chromatography-mass spectrometry (GC-MS) analysis on all tested e-liquids. Subsequent nonmetric multidimensional scaling (NMDS) analysis revealed that e-liquids are an extremely heterogeneous group. Furthermore, these data indicated that (i) the more chemicals contained in an e-liquid, the more toxic it was likely to be and (ii) the presence of vanillin was associated with higher toxicity values. Further analysis of common constituents by electron ionization revealed that the concentration of cinnamaldehyde and vanillin, but not triacetin, correlated with toxicity. We have also developed a publicly available searchable website (www.eliquidinfo.org). Given the large numbers of available e-liquids, this website will serve as a resource to facilitate dissemination of this information. Our data suggest that an HTS approach to evaluate the toxicity of multiple e-liquids is feasible. Such an approach may serve as a roadmap to enable bodies such as the Food and Drug Administration (FDA) to better regulate e-liquid composition.

High-Throughput Screening

10.1371/journal.pbio.2003904

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Citations (151)

Postron ionization mass spectrometry

D. L. Donohue Gary L. Glish Scott A. McLuckey L.D. Hulett H.S. McKown

Experiments are in progress to study the ionization of large molecules by slow positrons. Low energy positrons are being impinged on molecules such as butane, ethanol, benzene, toluene, and others. Masses of the resulting ions are being measured to study the fragmentation that follows the ionization processes. Studies will be made to determine if certain molecules or molecular groups can be selectively ionized by positron attachment. Results of this work are expected to lead to improved methods in analytical mass spectrometry. 4 refs., 3 figs.

Fragmentation

Atmospheric-pressure laser ionization

Thermal ionization

Source

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Supplementary Tables 1 - 4, Figures 1 - 2 from Peptide/MHC Tetramer–Based Sorting of CD8<sup>+</sup> T Cells to a Leukemia Antigen Yields Clonotypes Drawn Nonspecifically from an Underlying Restricted Repertoire

Sally A. Hunsucker Colleen S. McGary Benjamin G. Vincent Atim A. Enyenihi Jennifer P. Waugh

<p>The document contains 4 supplemental tables: 1. RT-PCR Primers for TCRbeta sequencing 2. HPLC-MS identified peptide sequences 3. The amino acid sequences of recurrent TCRbeta clonotypes 4. The distribution of the CDR3 amino acid length in the analyzed patient. There are 2 supplementary figures: 1. Tetramer flow cytometry showing the generation of an oligoclonal T-cell population with a UNC-CDK4-1 high affinity T-cell population. 2. More detailed information regarding the tetramer gating strategy employed.</p>

Tetramer

Cell Sorting

10.1158/2326-6066.22538669.v1

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Miniature Flow-Through Low-Temperature Plasma Ionization Source for Ambient Ionization of Gases and Aerosols

Analytical Chemistry (2015)

Sandra E. Spencer Miko Brandon G. Santiago Gary L. Glish

The design and operation of an inexpensive, miniature low-temperature plasma ion source is detailed. The miniature low-temperature plasma ion source is operated in a "flow-through" configuration, wherein the gaseous or aerosolized analyte, caffeine or pyrolyzed ethyl cellulose, in a carrier gas is used as the plasma gas. In this flow-through configuration, the sensitivity for the caffeine standard and the pyrolysis products of ethyl cellulose is maintained or increased and the reproducibility of the ion source is increased. Changes in the relative intensity of ions from the aerosol produced by pyrolysis of ethyl cellulose are observed in the mass spectrum when the low-temperature plasma ion source is used in the flow-through configuration. Experiments suggest this change in relative intensity is likely due to differences in ionization efficiency rather than increased fragmentation of ethyl cellulose pyrolysis products during ionization. Flow-through low-temperature plasma ionization with the miniature ion source is shown to be a promising technique for the ionization of compounds in gases or aerosol particles.

Ambient ionization

Thermal ionization

10.1021/acs.analchem.5b03447

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Citations (15)

Resolving powers of >7900 using linked scans: how well does resolving power describe the separation capability of differential ion mobility spectrometry

The Analyst (2015)

Brandon G. Santiago Rachel A. Harris Samantha L. Isenberg Gary L. Glish

Differential ion mobility spectrometry (DIMS) separations are described using similar terminology to liquid chromatography, capillary electrophoresis, and drift tube ion mobility spectrometry. The characterization and comparison of all these separations are typically explained in terms of resolving power, resolution, and/or peak capacity. A major difference between these separations is that DIMS separations are in space whereas the others are separations in time. However, whereas separations in time can, in theory, be extended infinitely, separations in space, such as DIMS separations, are constrained by the physical dimensions of the device. One method to increase resolving power of DIMS separations is to use helium in the DIMS carrier gas. However, ions have a greater mobility in helium which causes more ions to be neutralized due to collisions with the DIMS electrodes or electrode housing, i.e. the space constraints. This neutralization of ions can lead to the loss of an entire peak, or peaks, from a DIMS scan. To take advantage of the benefits of helium use while reducing ion losses, linked scans were developed. During a linked scan the amount of helium present in the DIMS carrier gas is decreased as the compensation field is increased. A comparison of linked scans to compensation field scans with constant helium is presented herein. Resolving powers >7900 are obtained with linked scans. However, this result highlights the limitation of using resolving power as a metric to describe DIMS separations.

Ion-mobility spectrometry

Drift tube

10.1039/c5an00845j

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Citations (9)

Structures of NO₃⁻ formed via glow discharge in atmospheric gases

Journal of the American Society for Mass Spectrometry (1990)

Rick A. Flurer Gary L. Glish Scott A. McLuckey

Tandem mass spectrometry experiments using both ion-molecule reactions and collision-induced dissociation (CID) in an ion trap mass spectrometer have been performed to characterize the structures of the NO3− ions that issue from an atmospheric sampling glow discharge ionization source. The experimental evidence suggests that significant abundances of at least two stable forms of NO3− can be obtained from an air-sustained glow discharge. Ab initio calculations have been performed to identify the structures of the likely isomers. Rate constants have been measured for the reaction of the less thermodynamically stable isomer(s) with carbon dioxide, and evidence is given for a new reaction, (OO−NO)− + NO2• → NO2− + O2 + NO•. The combination of em with a kinetic study is shown to be a useful approach for obtaining rate constant information when a mixture of reactive and unreactive isomers is present in the reactant population.

Glow discharge

10.1016/1044-0305(90)85038-n

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Citations (8)

Thermally Assisted Infrared Multiphoton Photodissociation in a Quadrupole Ion Trap

Analytical Chemistry (2001)

Anne H. Payne Gary L. Glish

Thermally assisted infrared multiphoton photodissociation (TA-IRMPD) provides an effective means to dissociate ions in the quadrupole ion trap mass spectrometer (QITMS) without detrimentally affecting the performance of the instrument. IRMPD can offer advantages over collision-induced dissociation (CID). However, collisions with the QITMS bath gas at the standard pressure and ambient temperature cause IR-irradiated ions to lose energy faster than photons can be absorbed to induce dissociation. The low pressure required for IRMPD (< or = 10(-5) Torr) is not that required for optimal performance of the QITMS (10(-3) Torr), and sensitivity and resolution suffer. TA-IRMPD is performed with the bath gas at an elevated temperature. The higher temperature of the bath gas results in less energy lost in collisions of the IR-excited ions with the bath gas. Thermal assistance allows IRMPD to be used at or near optimal pressures, which results in an approximately 1 order of magnitude increase in signal intensity. Unlike CID, IRMPD allows small product ions, those less than about one-third the m/z of the parent ion, to be observed. IRMPD should also be more easily paired with fluctuating ion sources, as the corresponding fluctuations in resonant frequencies do not affect IRMPD. Finally, while IR irradiation nonselectively causes dissociation of all ions, TA-IRMPD can be made selective by using axial expansion to move ions away from the path of the laser beam.

Infrared multiphoton dissociation

Quadrupole ion trap

Torr

10.1021/ac010245+

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Citations (90)