Aim: The 14-3-3η (eta) protein has been associated with the severity of the disease and joint destruction in patients with rheumatoid arthritis (RA). It has also been shown to be likely to be effective in inflammatory events. We aimed to investigate whether eta levels could be a potential biomarker in the diagnosis of ankylosing spondylitis (AS) and in the determination of disease activity in patients with AS.Methods: This study included 51 patients diagnosed with AS and 49 healthy controls aged 20-65 years. The routine hemogram, erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP) levels were measured and the neutrophil/lymphocyte ratio (NLR) was calculated in the patients. The serum eta levels were also measured in the patient and healthy control groups. The Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) and the Ankylosing Spondylitis Disease Activity Score (ASDAS) were used to assess disease activity. Sacroiliac joint radiographs of the patients were evaluated and the sacroiliitis was graded.Results: There was no statistically significant correlation between the degree of sacroiliitis, disease activity indices, and eta levels. There was no statistically significant correlation between eta levels and hematological parameters except for CRP. There was a negative, weak, and statistically significant relationship between the patients’ eta levels and CRP (r=-0.277; p=0.049). We could not find any correlation between the degree of sacroiliitis, disease activity indexes, and serum eta levels in AS patients.Conclusion: Serum eta levels are not a good biomarker for detecting disease activity in patients with ankylosing spondylitis. The 14-3-3η protein may play a more active role in rheumatic diseases where peripheral joint involvement is prominent.
Abstract Background Netrin-1 is a laminin class protein that guides the axonal during the first embryonic development, has pushing and pulling properties, and has axonal chemoattractant activity. Netrin-1 has been shown to increase the effect of fibrosis in mouse lung and human SSc lung cell cultures. In this study, we aimed to investigate the relationship between Netrin-1 and Systemic sclerosis (SSc) and to emphasize the role of Netrin-1 in the pathophysiology of SSc by increasing the known VEGF and M2 macrophage expression, which supports the fibrotic process. Methods The study included 56 SSc patients with a mean age of 48·.08±13.59 years and 58 healthy volunteers with a mean age of 48.01±11.59 years. SSc organ involvements were scanned retrospectively from patient files, and patients were grouped according to SSc complications. Calculation of Netrin-1 levels was performed using a quantitative sandwich enzyme immunoassay method with an ELISA kit (Elabscience, Texas, USA; catalog number: E-EL-H2328; lot number: GZWTKZ5SWK). The modified Rodnan skin score (mRSS) was used for skin thickness scoring in SSc patients. Results The median value of Netrin-1 was found to be significantly higher in SSc (268.8 [82.75-1006.64]) than in controls (108.63 [21.02-351.49]) (p<0.0001). In ROC analysis, a cut-off value of 354.24 for Netrin in SSc was found to provide a sensitive confidence interval with 32.8% sensitivity and 98.3% specificity (AUC[95% CI]: 0.746-0.895, p<0.0001). There was no significant correlation between Netrin-1 level, organ involvement in SSc, and mRSS (p>0.05). Conclusion We found a significant relationship between Netrin-1 levels and SSc disease in this study. Our study is the first clinical study in which Netrin-1 elevation was demonstrated in SSc patients.
ÖzAmaç: Behçet hastalığı (BH) ile iskemi modifiye albümin (İMA) ve tiyol molekülleri arasındaki ilişkiyi araştırmak ve BH'ye bağlı gelişebilecek komplikasyonlarda bu moleküllerin serum düzeylerini değerlendirmektir.BH rekürren oral aft, skar bırakan genital ülsere lezyonlar ve üveitin üçlü kompleks semptomları ile karakterize, histopatolojik olarak perivasküler dokuları ve vasküler duvarı tutan vaskülitik bir hastalıktır.Vasküler hasardan sorumlu mekanizmanın immünoregülatör sistem disregülasyonu ve oksidatif stres artışının olabileceği düşünülmektedir.Bu çalışmada BH'de ve hastalığın farklı klinik prezentasyonlarında İMA, native thiol
Objective Aim of this study is to compare the thiol/disulfide variables before treatment, at the 3rd and 6th months of biologic treatment in patients with axSpA.
Abstract Objective This study was designed to compare thiol/disulfide and ischemia-modified albumin (IMA) levels between psoriatic arthritis (PsA) and healthy controls and evaluate the correlation between these molecules and the disease activity scores used in PsA. Methods A total of 63 PsA patients and 49 healthy volunteers were included in the study. Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), modified disease activity score 28 (DAS28), and Bath Ankylosing Spondylitis Functional Index (BASFI) scores were used as disease activity indices for PsA patients. Calculations of native thiol (-SH), disulfide (-SS), and total thiol (-SH+-SS) molecules were made by the automatic spectrophotometric method, and the albumin cobalt binding test was used to measure IMA levels. Results In the PsA group, -SS/-SH and -SS/(-SH+-SS) levels were higher and -SH/(-SH+-SS) levels were lower than in controls. In the linear regression analysis, a significant correlation relationship was detected between DAS28-erythrocyte sedimentation rate (ESR) and -SS/(-SH+-SS) (β = 0.795, CI 95%, 0.196-1.395; P = .010), -SH/(-SH+-SS) (β = -0.475, CI 95%, 0.114-0.836; P = .010) and IMA (β = 3.932, CI 95%, 0.859-7.005; P = .013). Additionally, a significant correlation was detected between IMA and BASDAI and BASFI. Conclusion In PsA, thiol/disulfide homeostasis has shifted in favor of disulfide as an oxidative indicator. Serum thiol/disulfide levels are correlated with PsA disease activity indices.
The catabolism of tryptophan (Trp) in the kynurenine (Kyn) pathway is thought to have a critical immunosuppressive effect. To evaluate plasma Trp and metabolite levels to identify their diagnostic potential in rheumatoid arthritis (RA). 50 RA patients and 41 control were included in this study. The Trp and Kyn were analyzed and the Kyn\Trp ratio was calculated to estimate Indolamine 2,3 dioxygenase (IDO) enzyme activity involved in Trp degradation. The 50 patients had a mean age of 58·5 ± 10·6 years; 37 females and 13 males, F:M 2.8:1 and median disease duration was 10·1 (7–16) years. Rheumatoid factor was positive in 64 %. The median Trp value was significantly lower in RA (11120·7; 3259–16352 ng/ml) compared to control (12372·3; 7217–31,936 ng/ml) (p = 0·001). Kyn/Trp was significantly higher in RA (4·04; 2·5-12·3) compared to control (3.2; 2·1-4·7) (p < 0·0001). The median Kyn value was higher in RA (451·02; 264–1292 ng/ml) than in control (391·4; 236–1494 ng/ml) (p = 0·04). Trp significantly inversely correlated with the morning stiffness (r = −0·32, p = 0·025), Kyn significantly correlated with the C-reactive protein (CRP) (r = 0·31, p = 0·028) and erythrocyte sedimentation rate (ESR) (r = 0·41, p = 0·003) and the Kyn/Trp ratio correlated with the morning stiffness, CRP and ESR (r = 0·26, p = 0·045; r = 0·32, p = 0·025 and r = 0.32, p = 0·024 respectively). Kyn/Trp, Kyn and Trp significantly predicted RA at a cut-off value of 4.72, 589.1 ng/ml and 9921.1 ng/ml (p < 0.0001, p = 0.04 and p = 0.001 respectively). Our study showed that there is a significant relationship between RA and Kyn and Trp levels and IDO enzyme activity.
Özİdiyopatik granülomatöz mastit (İGM) tanısıyla takip edilen hastaların klinik, patolojik, laboratuvar ve radyolojik özelliklerini kullanarak tedavi yanıtlarını ve hastalık
Case report 1A 21-year-old male patient was diagnosed with axial spondyloarthritis according to ASAS diagnostic criteria upon the detection of bilateral sacroiliitis in sacroiliac magnetic resonance imaging (MRI) (Figure 1) at a physical therapy clinic, where he went to a physical therapy clinic with a complaint of low back pain with an inflammatory character for more than six weeks.Sulfasalazine 2x1000 mg and indomethacin 3x25 mg were started.In laboratory tests in this period white blood cell (WBC): 12000x10^9/L, neutrophil: 10000 x10^9/L, hemoglobin (Hmg): 9.1 g/dL, erythrocyte sedimentation rate (ESR):
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