본 연구에서는 상지에 배양한 상황버섯 균사체 유래 다당류의 기능성 식품 소재로의 활용 가능성을 확인하기 위해 조다당류의 분리 및 정제를 통해 분획을 얻었으며, 다양한 면역증강 효과를 확인하였다. 건조된 조다당류를 DEAE-sepharose CL-6B를 이용하여 크로마토그래피한 후 340 nm에서의 흡광도, 단백질, 당 및 uronic acid 함량을 분석한 결과, 증류수로 용출되는 비흡착 분획(PF-1, 분획번호 3~15)과 흡착된 후 NaCl 용액에 의해 용출되는 분획(PF-2, 분획번호 24~33)을 얻었다. 분획물 PF-1 및 PF-2의 유용성분 함량으로 총당 함량은 각각 75.51%, 52.38%, 총단백질 함량은 1.63%, 8.41%, uronic acid 함량은 17.53%, 15.04% 및 β-glucan 함량은 28.33%, 25.04%로 나타났다. PF-1 및 PF-2에 대해 대식세포주에 처리하여 세포생존율을 확인한 결과 100 μg/mL 농도까지 유의적으로 세포사멸이 나타나지 않아 세포독성이 없음을 확인할 수 있었다. Nitric oxide 생성량은 PF-1 100 μg/mL에서는 nitric oxide 생성량이 23.11 μM로 나타나 양성대조군으로 사용된 LPS 100 ng/mL 처리군(30.30 μM)의 약 76%의 효과가 있는 것으로 확인되었으며, cytokine 생성량(TNF-α 및 IL-6) 또한 무처리군에 비하여 높은 생성량을 나타내었다. Polymerase chain reaction을 통한 면역 관련 유전자 발현분석 결과, iNOS, COX-2, TNF-α, IL-6에서 PF-1 분획에서 높은 발현량을 나타내어 면역 증강을 목적으로 한 기능성식품 개발에 활용 가능하다고 판단된다.
본 연구는 생산형 녹지로서의 과수원을 대상으로 사과나무에 의한 탄소의 저장 및 연간 흡수를 산정하고, 재배과정에서 관리에 기인하여 발생하는 연간 탄소배출량을 파악하여 탄소저감 효과를 계량화하였다. 연구대상 과수원에서 유목에서 성목에 이르는 일정 간격의 근원직경 크기를 고려한 수목을 구입하여, 근굴취를 포함하는 직접수확법에 의해 개체당 부위별 및 전체 생체량을 산정하고 탄소저장량을 산출하였다. 근원부의 줄기 원판을 채취하여 직경생장을 분석하고 연간 탄소흡수량을 산정하였다. 관리에 따른 연간 탄소배출은 전정, 제초, 관수, 시비, 병충해 방제 등을 포함하는 관리자료의 구득, 관리자 면담 및 부분 실측을 바탕으로 계량화하였다. 근원직경을 독립변수로 생장에 따른 사과나무 단목의 탄소저장과 연간 탄소흡수를 계량화하는 활용 용이한 계량모델을 개발하였다. 사과나무의 탄소저장량과 연간 탄소흡수량은 모두 직경생장과 더불어 증가하였고, 직경급 간 그 차이도 직경이 커질수록 증가하는 경향이었다. 근원직경 10 및 15cm인 사과나무 단목은 각각 9.1 및 21.0kg의 탄소를 저장하고, 연간 1.0 및 1.6kg의 탄소를 흡수하는 것으로 나타났다. 연구대상 과수원의 단위면적당 탄소저장량과 연간 탄소흡수량은 각각 3.81t/ha, 0.42t/ha/년이었고, 연간 탄소배출량은 1.30t/ha/년이었다. 즉, 관리 관련 연간 탄소배출량은 연간 탄소흡수량보다 약 3배 더 많았다. 연구결과를 토대로, 생산형 녹지에 적용 가능한 관수, 농약 및 비료의 효율적 적용을 포함하는 저탄소형 관리방안을 모색하였다. 본 연구는 아직 부진했던 뿌리 생체량의 실측과 탄소배출의 구체적 인벤토리를 통해 탄소계량 관련 정보를 구축하는 새로운 초석을 제공한다. This study quantified the storage and annual uptake of carbon by apple trees in orchards as a production-type greenspace, and computed the annual carbon emissions from apple cultivation. Tree individuals in the study orchards were sampled to include the range of stem diameter sizes. The study measured biomass for each part including the roots of sample trees through a direct harvesting method to compute total carbon storage per tree. Annual carbon uptake per tree was quantified by analyzing the radial growth rates of stem samples at ground level. Annual carbon emissions from management practices such as pruning, mowing, irrigation, fertilization, and use of pesticides and fungicides were estimated based on maintenance data, interviews with managers, and actual measurements. Regression models were developed using stem diameter at ground level (D) as an independent variable to easily estimate storage and annual uptake of the carbon. Storage and annual uptake of carbon per tree increased as D sizes got larger. Apple trees with D sizes of 10 and 15 cm stored 9.1 and 21.0 kg of carbon and annually sequestered 1.0 and 1.6 kg, respectively. Storage and annual uptake of carbon per unit area in study orchards were 3.81 t/ha and 0.42 t/ha/yr, respectively, and annual carbon emissions were 1.30 t/ha/yr. Thus, the carbon emissions were about 3 times greater than the annual carbon uptake. The study identified management practices to reduce the carbon footprint of production-type greenspace, including efficient uses of water, pesticides, fungicides, and fertilizers. It breaks new ground by including measured biomass of roots and a detailed inventory of carbon emissions.
In this paper, we propose an antenna module with high isolation between Tx and Rx in RFID reader module. The proposed module consists of a quadrafilar antenna and a directional coupler with a switchable dummy load to improve the directivity. The proposed module achieves the isolation higher than 20 ㏈ between Tx and Rx. To show the validity of the proposed scheme, we have performed the measurement of tagging range and multi-tagging ability. The experiment results show that the detecting range and multi-tagging ability are enhanced by 81% and 200%, respectively.
Landscape trees sequester carbon during their growth processes, but they emit carbon through production in nurseries, which may offset carbon uptake. This study quantified the carbon footprint of landscape tree production. After determining the scope of life cycle for landscape tree production, the energy and material used to produce trees of a target size were analyzed by conducting a field survey of 35 nurseries. This energy consumption and input material were converted to an estimate of carbon emitted using data on carbon emission coefficients. The net carbon uptake was 4.6, 12.2, and 24.3 kg/tree for trees with a DBH of 7, 10, and 13 cm, respectively. Thus, even though carbon is emitted during the production process, landscape trees can act as a source of carbon uptake in cities that have high energy consumption levels. This study broke new ground for quantifying the carbon footprint of landscape tree production by overcoming limitations of the past studies that only considered carbon uptake due to absence of data on energy consumption and difficulty of field survey. These study results are expected to provide information on the carbon footprint of landscape trees and to be useful in determining optimal greenhouse gas emissions reduction goal through urban greenspaces.
Abstract We report the integration of the linkage map of tomato chromosome 2 with a high-density bacterial artificial chromosome fluorescence in situ hybridization (BAC–FISH)-based cytogenetic map. The euchromatic block of chromosome 2 resides between 13 and 142 cM and has a physical length of 48.12 μm, with 1 μm equivalent to 540 kb. BAC–FISH resolved a pair of loci that were 3.7–3.9 Mb apart and were not resolved on the linkage map. Most of the regions had crossover densities close to the mean of ∼200 kb/cM. Relatively hot and cold spots of recombination were unevenly distributed along the chromosome. The distribution of centimorgan/micrometer values was similar to the previously reported recombination nodule distribution along the pachytene chromosome. FISH-based physical maps will play an important role in advanced genomics research for tomato, including map-based cloning of agronomically important traits and whole-genome sequencing.
This study quantified annual uptake and storage of carbon by urban greenspace in institutional lands and suggested improvement of greenspace structures to enhance carbon reduction effects. The study selected a total of five study cities including Seoul, Daejeon, Daegu, Chuncheon, and Suncheon, based on areal size and nationwide distribution. Horizontal and vertical greenspace structures were field-surveyed, after institutional greenspace lots were selected using a systematic random sampling method on aerial photographs of the study cities. Annual uptake and storage of carbon by woody plants were computed applying quantitative models of each species developed for urban landscape trees and shrubs. Tree density and stem diameter (at breast height) in institutional lands averaged 1.4±0.1 trees/100 m2 and 14.9±0.2 cm across the study cities, respectively. Of the total planted area, the ratio of single-layered planting only with trees, shrubs, or grass was higher than that of multi-layered structures. Annual uptake and storage of carbon per unit area by woody plants averaged 0.65±0.04 t/ha/yr and 7.37±0.47 t/ha, which were lower than those for other greenspace types at home and abroad. This lower carbon reduction was attributed to lower density and smaller size of trees planted in institutional lands studied. Nevertheless, the greenspace in institutional lands annually offset carbon emissions from institutional electricity use by 0.6 (Seoul)~1.9% (Chuncheon). Tree planting in potential planting spaces was estimated to sequester additionally about 18% of the existing annual carbon uptake. Enhancing carbon reduction effects requires active tree planting in the potential spaces, multi-layered/clustered planting composed of the upper trees, middle trees and lower shrubs, planting of tree species with greater carbon uptake capacity, and avoidance of the topiary tree maintenance. This study was focused on finding out greenspace structures and carbon offset levels in institutional lands on which little had been known.
The physicochemical properties of spray-dried rice flour with Lactobacillus plantarum CGKW3 were investigated. Amylose and damaged starch contents of spray-dried rice flour (S10, S20, S30, and S50) with L. plantarum CGKW3 were 14.18~17.75% and 24.65~34.08%, respectively. The particle size of spray-dried rice flour was 82.28~131.17 μm. The rice flour with L. plantarum CGKW3 showed a good powder flowability. The water absorption and water solubility of spray-dried rice flour were 1.96~2.13 and 9.91~21.95%, respectively. Thermal properties measured by differential scanning calorimeter revealed that the enthalpy (△H) for starch gelatinization were highest in the rice flour (S50) with L. plantarum CGKW3. When compared, the viable cell number of spray-dried rice flour were found to be in the following order: S10 (5.78 log CFU/g) ‹ S20 (6.38 log CFU/g) ‹ S30 (6.69 log CFU/g) ‹ S50 (7.11 log CFU/g). The survaival rate of L. plantarum CGKW3 was 60.02-73.85%, which reflected the improvement in the quality of rice flour with an increase in treatment concentration. Based on our results, spray-dried rice flour with L. plantarum CGKW3 could be used in various types of rice foods.
In this paper, we have designed and manufactured 10MHz power source for the application of short distance wireless power transmission. The designed power source consists of a DDS(direct digital synthesizer) signal generator, a buffer driver and a balanced power amplifier. Short range wireless power transmission is usually carried out by near-field inductive coupling between source and load. The distance variation between source and load gives rise to the change of load impedance of power amplifier, which has effect on the operation of power amplifier. To overcome this problem due to load variation of power amplifier, we have adopted the balanced power amplifier using the quadrature hybrid implemented by lumped capacitors and a mutually coupled coil. The experiment results show the above 40dBm output power, frequency range of 9 to 11MHz, and total DC power consumption of 36W.
In this paper, we present an antenna front-end architecture of UHF RFID reader dedicated for the near field applications. For the TX-RX isolation, the presented architecture adopts the sum-difference hybrid scheme which operates as a difference mode (or balun) and common-mode for TX and RX operations, respectively. The validity of the proposed architecture is demonstrated by applying to the smart shelf which identifies the books with UHF-tags. Compared with the conventional architecture using a directional coupler or a circulator, the test result shows the enhanced performance in sensitivity and multi-tagging ability.
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