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Beatrica Ševčı́ková

Slovak Academy of Sciences

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Ján Kormanec

Slovak Academy of Sciences

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Bronislava Řežuchová

Slovak Academy of Sciences

Renáta Nováková

Slovak Academy of Sciences

Dagmar Homerová

Slovak Academy of Sciences

Lubomira Feckova

Slovak Academy of Sciences

Dominika Csolleiova

Slovak Academy of Sciences

Erik Mingyar

Slovak Academy of Sciences

Rachel Javorova

Slovak Academy of Sciences

Luz Elena Núñez

EntreChem (Spain)

Jesús Cortés

EntreChem (Spain)

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Slovak Academy of Sciences

Institute of Molecular Biology of the Slovak Academy of Sciences

Swansea University

Comenius University Bratislava

Czech Academy of Sciences, Biology Centre

Institute of Chemistry of the Slovak Academy of Sciences

Bielefeld University

Biomedical Research Center of the Slovak Academy of Sciences

EntreChem (Spain)

Czech Academy of Sciences, Institute of Microbiology

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Cascade of sigma factors in streptomycetes: identification of a new extracytoplasmic function sigma factor σJ that is under the control of the stress-response sigma factor σH in Streptomyces coelicolor A3(2)

Archives of Microbiology (2006)

Vladislava Mazurakova Beatrica Ševčı́ková Bronislava Řežuchová Ján Kormanec

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10.1007/s00203-006-0158-9

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Citations (19)

Identification of DNA-binding proteins involved in regulation of expression of the Streptomyces aureofaciens sigF gene, which encodes sporulation sigma factor σF

Microbiology (2000)

Dagmar Homerová Beatrica Ševčı́ková Ondrej Sprušanský Ján Kormanec

Expression of the sigF gene encoding a sporulation-specific sigma factor, σF, in Streptomyces aureofaciens is restricted only to sporulation. Gel mobility-shift assays using protein fractions from different developmental stages of S. aureofaciens revealed two different putative proteins specifically bound to the sigF promoter region: a protein (designated RsfA) present in young substrate mycelium, and a protein (designated RsfB) present in the course of sporulation. Based on the characteristic profiles of their appearance during differentiation, RsfA might be a repressor and RsfB an activator of sigF expression. The location of a specific binding site of the repressor-like protein (RsfA) was determined by gel mobility-shift assays of promoter deletion fragments and by DNase I footprinting analysis. The binding site mapped from nucleotides −87 to −25 relative to the transcription start point of the sigF promoter, and overlapped the −35 promoter region. Given the dependence of sigF expression upon whiH, the putative sporulation transcription factor WhiH was overproduced in Escherichia coli and used in the mobility-shift assays with the sigF promoter. However, no specific binding was detected, indicating an indirect dependence of sigF upon whiH.

Streptomyces aureofaciens

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DNA footprinting

10.1099/00221287-146-11-2919

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Phenotypic analysis of Salmonella enterica serovar Typhimurium rpoE mutants encoding RNA polymerase extracytoplasmic stress response sigma factors σE with altered promoter specificity

Archives of Microbiology (2012)

Bronislava Řežuchová Dagmar Homerová Beatrica Ševčı́ková Renáta Nováková Lubomira Feckova

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Salmonella enterica

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Specificity factor

SOS response

10.1007/s00203-012-0843-9

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A Structural Analysis of the Angucycline-Like Antibiotic Auricin from Streptomyces lavendulae Subsp. Lavendulae CCM 3239 Revealed Its High Similarity to Griseusins

Antibiotics (2019)

Mária Matulová Lubomira Feckova Renáta Nováková Erik Mingyar Dominika Csolleiova

We previously identified the aur1 gene cluster in Streptomyces lavendulae subsp. lavendulae CCM 3239 (formerly Streptomyces aureofaciens CCM 3239), which is responsible for the production of the angucycline-like antibiotic auricin (1). Preliminary characterization of 1 revealed that it possesses an aminodeoxyhexose d-forosamine and is active against Gram-positive bacteria. Here we determined the structure of 1, finding that it possesses intriguing structural features, which distinguish it from other known angucyclines. In addition to d-forosamine, compound 1 also contains a unique, highly oxygenated aglycone similar to those of spiroketal pyranonaphthoquinones griseusins. Like several other griseusins, 1 also undergoes methanolysis and displays modest cytotoxicity against several human tumor cell lines. Moreover, the central core of the aur1 cluster is highly similar to the partial gris gene cluster responsible for the biosynthesis of griseusin A and B in both the nature of the encoded proteins and the gene organization.

Streptomyces aureofaciens

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Aglycone

10.3390/antibiotics8030102

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Cross-Recognition of Promoters by the Nine SigB Homologues Present in Streptomyces coelicolor A3(2)

International Journal of Molecular Sciences (2021)

Beatrica Ševčı́ková Bronislava Řežuchová Vladimira Mazurakova Dagmar Homerová Renáta Nováková

In contrast to Bacillus subtilis, Streptomyces coelicolor A3(2) contains nine homologues of stress response sigma factor SigB with a major role in differentiation and osmotic stress response. The aim of this study was to further characterize these SigB homologues. We previously established a two-plasmid system to identify promoters recognized by sigma factors and used it to identify promoters recognized by the three SigB homologues, SigF, SigG, and SigH from S. coelicolor A3(2). Here, we used this system to identify 14 promoters recognized by SigB. The promoters were verified in vivo in S. coelicolor A3(2) under osmotic stress conditions in sigB and sigH operon mutants, indicating some cross-recognition of these promoters by these two SigB homologues. This two-plasmid system was used to examine the recognition of all identified SigB-, SigF-, SigG-, and SigH-dependent promoters with all nine SigB homologues. The results confirmed this cross-recognition. Almost all 24 investigated promoters were recognized by two or more SigB homologues and data suggested some distinguishing groups of promoters recognized by these sigma factors. However, analysis of the promoters did not reveal any specific sequence characteristics for these recognition groups. All promoters showed high similarity in the -35 and -10 regions. Immunoblot analysis revealed the presence of SigB under osmotic stress conditions and SigH during morphological differentiation. Together with the phenotypic analysis of sigB and sigH operon mutants in S. coelicolor A3(2), the results suggest a dominant role for SigB in the osmotic stress response and a dual role for SigH in the osmotic stress response and morphological differentiation. These data suggest a complex regulation of the osmotic stress response in relation to morphological differentiation in S. coelicolor A3(2).

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10.3390/ijms22157849

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Recent achievements in the generation of stable genome alterations/mutations in species of the genus Streptomyces

Applied Microbiology and Biotechnology (2019)

Ján Kormanec Bronislava Řežuchová Dagmar Homerová Dominika Csolleiova Beatrica Ševčı́ková

10.1007/s00253-019-09901-0

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Cross-Recognition of the ssgBp Promoter, Which Controls the Expression of the Sporulation–Specific Cell Division Gene ssgB, by Nine SigB Homologues in Streptomyces coelicolor A3(2)

Beatrica Ševčı́ková Bronislava Řežuchová Renáta Nováková Filip Opaterny Rachel Javorova

In their natural environment, bacteria are exposed to various stresses. The stress-response sigma factor SigB of gram-positive Bacillus subtilis is the best-characterized example. Unlike Bacillus subtilis, the gram-positive bacterium Streptomyces coelicolor A3(2) contains nine SigB homologues (SigBFGHIKLMN) with a major role in differentiation and response to osmotic stress. We previously constructed a two-plasmid system to identify promoters recognized by these sigma factors. Interestingly, almost all identified promoters were recognized by two or more SigB homologues. However, no specific sequences characteristic for these recognition groups were found. To examine this cross-recognition in vivo in S. coelicolor A3 (2), one of these promoters was cho-sen, which drives the expression of the sporulation-specific gene ssgB. The ssgBp promoter was inserted into a luciferase reporter plasmid and conjugated to S. coelicolor M145 and nine mutant strains containing deleted individual sigB homologous genes. Luciferase reporter activity indi-cated differential activity of this promoter in these mutant strains, suggesting overlapping pro-moter recognition by these SigB homologues. To determine which nucleotides in the ̶ 10 re-gion are responsible for the selection of a specific SigB homologue, several mutant promoters with altered last three nucleotides in this region were prepared and tested in the two-plasmid system. Some mutant promoters were specifically recognized by some SigB homologues. Mutant promoters were inserted into a luciferase reporter plasmid and conjugated to S. coelicolor A3(2) and these nine mutant strains. Luciferase reporter activity indicated differential activity of these ssgBp mutant promoters, indicating overlapping promoter recognition by these SigB homologues in S. coelicolor A3(2).

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10.20944/preprints202309.0591.v1

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Citations (1)

Pleiotropic anti-anti-sigma factor BldG is phosphorylated by several anti-sigma factor kinases in the process of activating multiple sigma factors in Streptomyces coelicolor A3(2)

Gene (2020)

Beatrica Ševčı́ková Bronislava Řežuchová Erik Mingyar Dagmar Homerová Renáta Nováková

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10.1016/j.gene.2020.144883

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Citations (9)