Ivón Menéndez

Centro de Ingeniería Genética y Biotecnología

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Viviana Falcón

Centro de Ingeniería Genética y Biotecnología

Nelson Acosta‐Rivero

Centro de Ingeniería Genética y Biotecnología

Santiago Dueñas‐Carrera

Centro de Ingeniería Genética y Biotecnología

Bienvenido Grá

Instituto de Gastroenterología

Maria Cristina De Rosa

Istituto di Scienze e Tecnologie Chimiche "Giulio Natta"

Glay Chinea

Centro de Ingeniería Genética y Biotecnología

Juan B. Kourí

Center for Research and Advanced Studies of the National Polytechnic Institute

Mineko Shibayama

Instituto Politécnico Nacional

José Luna‐Muñoz

Universidad Nacional Pedro Henríquez Ureña

Jorge Gavilondo

Centro de Ingeniería Genética y Biotecnología

Cooperative Institutions

Centro de Ingeniería Genética y Biotecnología

National Center for Genetic Engineering and Biotechnology

Instituto de Medicina Tropical “Pedro Kourí”

Instituto de Gastroenterología

Center for Research and Advanced Studies of the National Polytechnic Institute

Cuban Neuroscience Center

Instituto Politécnico Nacional

University of Camagüey

Applied Scientific Research (United States)

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Nuclear localization of nucleocapsid-like particles and HCV core protein in hepatocytes of a chronically HCV-infected patient

Biochemical and Biophysical Research Communications (2003)

Viviana Falcón Nelson Acosta‐Rivero Glay Chinea Maria Cristina De Rosa Ivón Menéndez

Immunoelectron microscopy

Viral life cycle

10.1016/j.bbrc.2003.08.118

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Citations (29)

A histopathological contribution to the diagnosis of vitamin A deficiency in calves

E. Torroella Raquel Camara Rivero M. Lavischka Norberto Herrera Merino Ivón Menéndez

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Evidences of Hepatitis C Virus Replication in Hepatocytes and Peripheral Blood Monocuclear Cells from Patients Negative for Viral RNA in Serum

American Journal of Infectious Diseases (2005)

Viviana Falcón Nelson Acosta‐Rivero Nelson Acosta‐Rivero José Luna‐Muñoz Magdalena Miranda-Sa

In this study, we examined the expression of hepatitis C virus (HCV) components in hepatocytes and peripheral blood mononuclear cells (PBMC) from patients positive for anti-HCV antibodies and negative for serum HCV-RNA. The samples obtained from 25 randomly selected patients (13 of 25 patients showed no histological evidences of chronic hepatitis and had normal serum ALAT and GGTP levels) were studied by in situ Hybridization and Immunofluorescence assays. The findings show that HCV-RNA of both positive and negative polarity was carried in the hepatocytes of more than 80% of cases. The proportion of cells expressing the negative strand (mean ± SD, 10.25% ± 5.56%) was lower than those expressing positive strand (mean ± SD, 19.88% ± 9.19%) (p=0.0076; Student’s t test). In addition, reaction products suggestive of HCV core, E1 and E2 antigens within hepatocytes were also observed. Both hybridization and immunofluorescence signals were localized in the cytoplasm suggesting that this is the place of active HCV replication. On the other hand, the HCV-RNA of positive polarity was detected in PBMC from 16 out of 17 samples analyzed (94%) while the HCV-RNA of negative polarity was detected in 82% of samples investigated. Positive hybridization signals were localized in the cytoplasm of PBMC. Interestingly, 12 out of 13 patients with clinical and histological resolution of hepatitis, contain HCV-RNA in either liver or PBMC. These results provide further evidences that the intermediate replicative form of the HCV genome can persist in hepatocytes and PBMC after apparently complete resolution of chronic hepatitis C.

Replication

10.3844/ajidsp.2005.34.42

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Ultrastructural Evidences of Hepatitis B Infection in Human Liver Biopsies Disclose Complex Assembly and Morphogenesis Pathways for Hepatitis B Virus

American Journal of Infectious Diseases (2008)

Viviana Falcón Ivón Menéndez Nelson Acosta‐Rivero Mineko Shibayama Marlia-C de la Rosa

Despite of recent advances on acknowledge of hepatitis B virus (HBV) structure and biology, little is known about the morphogenesis and release of virions. In this study, the ultrastructural analysis of HBV components in liver biopsies from chronically HBV-infected patients disclosed complex assembly and morphogenesis pathways for HBV. HBV core (HBcAg) and surface (HBsAg) antigens were specifically identified in all liver biopsies from HVB-infected patients. HBcAg containing core-like particles 24-28 nm in diameter were observed both in nucleus and cytoplasm of hepatocytes. Dane’s-like particles were detected either budding to or into the lumen of ER close to HBsAg containing tubular structures. Besides, Dane’s-like particles were detected in different vesicular compartments resembling multivesicular endosomes. Other kind of enveloped HBV-like particles similar to the previously described cobra-shaped and horn-shaped particles were also observed in hepatocytes. Some of these particles were connected to the vesicle membrane through a stalk 20-22 nm in diameter. On the other hand, spherical subviral particles (SVP) were frequently observed in cytoplasmic vesicles. Moreover, a minor proportion of enveloped HBV-like particles budding through the plasma membrane to the extracellular space and bile canaliculi were detected. Interestingly, Dane’s-like particles in the bile canaliculi and entering into ductular-like cells were shown. Strikingly, Dane’s-like particles close to tubular structures and specific immunolabeling for HBcAg both in cytoplasm and nuclei of stellate-like cells were detected. Remarkably, HVB-like particles appeared entering hepatocytes from large cytoplasmic processes of fibrocytes raising the interesting possibility of a cell to cell passage of HBV through direct transcellular migration.

Hepatitis virus

10.3844/ajidsp.2008.96.103

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Aportes al estudio de los mecanismos patogénicos del VHC y sus implicaciones en la comprensión de la enfermedad

Viviana Falcón Santiago Dueñas Ivón Menéndez Maria Cristina De Rosa Juan Morales

Se estudiaron por microscopia electronica de transmision y confocal, biopsias hepaticas y celulas mononucleares de sangre periferica (CMSP) en 150 pacientes cubanos con hepatitis C cronica. Se hallaron elementos novedosos acerca de la deteccion de la capsida del VHC en celulas extraparenquimatosas del higado. Se visualizo la presencia de apoptosis en las celulas hepaticas y en CMSP mediante la tecnica TUNEL y la activacion de efectores de la apoptosis. Se observo por primera vez, la presencia de ARN de cadena positiva y negativa en hepatocitos y en CMSP en mas del 80% de las muestras analizadas de pacientes ARN negativo en suero, con mejoria histologica y bioquimica. Se mostro por primera vez, que las celulas extraparenquimatosas del higado y las CMSP constituyen reservorios para la replicacion del VHC. La presencia en las celulas estrelladas del AgcHC y de las particulas del nucleo del VHC, detectadas por primera vez, mostraron que el VHC puede infectar directamente a estas celulas y su transdiferenciacion en celulas similares a miofibroblastos, lo que modula asi el proceso de fibrogenesis hepatica y demuestra que junto a los fibroblastos, las celulas estrelladas desempenan una funcion relevante en la fibrosis hepatica. La aplicacion de estos conocimientos tiene implicaciones importantes para el diagnostico, seguimiento y pronostico de la enfermedad de referencia

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Detection of HCV-related Structures in the Mitochondria of Apoptotic Hepatocytes in Liver Biopsies from Chronically HCV-infected Patients

Microscopy and Microanalysis (2003)

Viviana Falcón Nelson Acosta‐Rivero Glay Chinea Jorge Gavilondo Marı́a-C de la Rosa

Journal Article Detection of HCV-related Structures in the Mitochondria of Apoptotic Hepatocytes in Liver Biopsies from Chronically HCV-infected Patients Get access Viviana Falcón, Viviana Falcón Biomedicine Division, Center for Genetic Engineering and Biotechnology., P.O. Box 6162, C.P. 10600, Havana. Email: viviana.falcon@cigb.edu.cu Search for other works by this author on: Oxford Academic Google Scholar Nelson Acosta-Rivero, Nelson Acosta-Rivero Biomedicine Division, Center for Genetic Engineering and Biotechnology., P.O. Box 6162, C.P. 10600, Havana. Email: viviana.falcon@cigb.edu.cu Search for other works by this author on: Oxford Academic Google Scholar Glay Chinea, Glay Chinea Biomedicine Division, Center for Genetic Engineering and Biotechnology., P.O. Box 6162, C.P. 10600, Havana. Email: viviana.falcon@cigb.edu.cu Search for other works by this author on: Oxford Academic Google Scholar Jorge Gavilondo, Jorge Gavilondo Biomedicine Division, Center for Genetic Engineering and Biotechnology., P.O. Box 6162, C.P. 10600, Havana. Email: viviana.falcon@cigb.edu.cu Search for other works by this author on: Oxford Academic Google Scholar María-C de la Rosa, María-C de la Rosa Biomedicine Division, Center for Genetic Engineering and Biotechnology., P.O. Box 6162, C.P. 10600, Havana. Email: viviana.falcon@cigb.edu.cu Search for other works by this author on: Oxford Academic Google Scholar Ivón Menéndez, Ivón Menéndez Biomedicine Division, Center for Genetic Engineering and Biotechnology., P.O. Box 6162, C.P. 10600, Havana. Email: viviana.falcon@cigb.edu.cu Search for other works by this author on: Oxford Academic Google Scholar Santiago Dueñas-Carrera, Santiago Dueñas-Carrera Biomedicine Division, Center for Genetic Engineering and Biotechnology., P.O. Box 6162, C.P. 10600, Havana. Email: viviana.falcon@cigb.edu.cu Search for other works by this author on: Oxford Academic Google Scholar Ariel Viña, Ariel Viña Biomedicine Division, Center for Genetic Engineering and Biotechnology., P.O. Box 6162, C.P. 10600, Havana. Email: viviana.falcon@cigb.edu.cu Search for other works by this author on: Oxford Academic Google Scholar Bienvenido Gra, Bienvenido Gra Institute of Gastroenterology, C.P. 10400, Havana; C.P. 10400, Havana Search for other works by this author on: Oxford Academic Google Scholar Miriam Noa, Miriam Noa National Center for Scientific Research, P.O. Box 6990, Havana Search for other works by this author on: Oxford Academic Google Scholar ... Show more Felix Alvarez, Felix Alvarez Biomedicine Division, Center for Genetic Engineering and Biotechnology., P.O. Box 6162, C.P. 10600, Havana. Email: viviana.falcon@cigb.edu.cu Search for other works by this author on: Oxford Academic Google Scholar Josefina Rodríguez, Josefina Rodríguez Biomedicine Division, Center for Genetic Engineering and Biotechnology., P.O. Box 6162, C.P. 10600, Havana. Email: viviana.falcon@cigb.edu.cu Search for other works by this author on: Oxford Academic Google Scholar Juan Morales Juan Morales Biomedicine Division, Center for Genetic Engineering and Biotechnology., P.O. Box 6162, C.P. 10600, Havana. Email: viviana.falcon@cigb.edu.cu Search for other works by this author on: Oxford Academic Google Scholar Microscopy and Microanalysis, Volume 9, Issue S02, 1 August 2003, Pages 1424–1425, https://doi.org/10.1017/S1431927603447120 Published: 19 July 2003

10.1017/s1431927603447120

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HCV core protein localizes in the nuclei of nonparenchymal liver cells from chronically HCV-infected patients

Biochemical and Biophysical Research Communications (2005)

Viviana Falcón Nelson Acosta‐Rivero Mineko Shibayama Glay Chinea Jorge Gavilondo

Hepatic stellate cell

Pathogenesis

Immunostaining

10.1016/j.bbrc.2005.02.107

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Citations (22)

Regional conformational change involving phosphorylation of tau protein at the Thr231, precedes the structural change detected by Alz-50 antibody in Alzheimer's disease

Journal of Alzheimer s Disease (2005)

José Luna‐Muñoz Francisco García‐Sierra Viviana Falcón Ivón Menéndez Laura Chávez-Macías

Neurofibrillary tangles (NFTs) are the neuropathological hallmarks in Alzheimer's disease (AD). Densities of NFTs correlate with the dementia status. NFTs reflect the intracellular accumulation of abnormal paired helical filaments (PHFs) composed of

Tau protein

Neurofibrillary tangle

Conformational change

10.3233/jad-2005-8104

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Citations (100)

Ultrastructural evidences of HCV infection in hepatocytes of chronically HCV-infected patients

Biochemical and Biophysical Research Communications (2003)

Viviana Falcón Nelson Acosta‐Rivero Glay Chinea Jorge Gavilondo Marı́a-C de la Rosa

Immunoelectron microscopy

10.1016/s0006-291x(03)00884-2

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