In the study,p30 gene of Avian reticuloendotheliosis virus(REV) was amplified by PCR from provirus genomic cDNA clone of SNV strain.The p30 gene was then subcloned into the pCold-HF vector.The resulting recombinant plasmid(pCP30) was transformed into the competent cells BL21(DE3).With induction of isopropylthio-β-D-galactoside(IPTG),soluble p30 protein was expressed in BL21 cells and visualized as a band of 72 kDa in SDS-PAGE.Six-week old BALB/c mice were immunized with the purified protein to prepare p30 antiserum.The reactivity of the antiserum was determined in Western blot and indirect immunofluorescent assay.The availability of p30 antiserum would benefit to develop diagnostic method for the detection of REV infection in chickens and waterfowls.
Journal Article Construction of a cell‐surface display system based on the N‐terminal domain of ice nucleation protein and its application in identification of mycoplasma adhesion proteins Get access S. Bao, S. Bao Shanghai Veterinary Research Institute Chinese Academy of Agricultural Sciences Shanghai ChinaCollege of Veterinary Medicine Gansu Agricultural University Lanzhou China Search for other works by this author on: Oxford Academic Google Scholar S. Yu, S. Yu Shanghai Veterinary Research Institute Chinese Academy of Agricultural Sciences Shanghai China Search for other works by this author on: Oxford Academic Google Scholar X. Guo, X. Guo College of Veterinary Medicine Gansu Agricultural University Lanzhou China Search for other works by this author on: Oxford Academic Google Scholar F. Zhang, F. Zhang Shanghai Veterinary Research Institute Chinese Academy of Agricultural Sciences Shanghai China Search for other works by this author on: Oxford Academic Google Scholar Y. Sun, Y. Sun Shanghai Veterinary Research Institute Chinese Academy of Agricultural Sciences Shanghai China Search for other works by this author on: Oxford Academic Google Scholar L. Tan, L. Tan Shanghai Veterinary Research Institute Chinese Academy of Agricultural Sciences Shanghai China Search for other works by this author on: Oxford Academic Google Scholar Y. Duan, Y. Duan Shanghai Veterinary Research Institute Chinese Academy of Agricultural Sciences Shanghai China Search for other works by this author on: Oxford Academic Google Scholar F. Lu, F. Lu College of Veterinary Medicine Gansu Agricultural University Lanzhou China Search for other works by this author on: Oxford Academic Google Scholar X. Qiu, X. Qiu Shanghai Veterinary Research Institute Chinese Academy of Agricultural Sciences Shanghai China Search for other works by this author on: Oxford Academic Google Scholar C. Ding C. Ding Shanghai Veterinary Research Institute Chinese Academy of Agricultural Sciences Shanghai China Correspondence Chan Ding, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 518 Ziyue Road, Shanghai 200241, China. E‐mail: shoveldeen@shvri.ac.cn Search for other works by this author on: Oxford Academic Google Scholar Journal of Applied Microbiology, Volume 119, Issue 1, 1 July 2015, Pages 236–244, https://doi.org/10.1111/jam.12824 Published: 01 July 2015 Article history Received: 15 September 2014 Revision received: 30 March 2015 Accepted: 31 March 2015 Published: 01 July 2015
Viruses have evolved different strategies to hijack subcellular organelles during their life cycle to produce robust infectious progeny. Successful viral reproduction requires the precise assembly of progeny virions from viral genomes, structural proteins, and membrane components. Such spatial and temporal separation of assembly reactions depends on accurate coordination among intracellular compartmentalization in multiple organelles. Here, we overview the rearrangement and morphology remodeling of virus-triggered intracellular organelles. Focus is given to the quality control of intracellular organelles, the hijacking of the modified organelle membranes by viruses, morphology remodeling for viral replication, and degradation of intracellular organelles by virus-triggered selective autophagy. Understanding the functional reprogram and morphological remodeling in the virus-organelle interplay can provide new insights into the development of broad-spectrum antiviral strategies.
Infectious bronchitis (IB) and Newcastle disease (ND) are two major infectious diseases that are a threat to the domestic poultry industry. In this study, we successfully generated a recombinant LaSota candidate vaccine strain, rNDV-IBV-T/B, which expresses a short, synthetic, previously identified IBV S1 multi-epitope cassette using the reverse genetic system. The recombinant virus was propagated in nine-day-old embryonated chicken eggs for 20 passages and genetic stability was confirmed by whole genome DNA sequencing. The recombinant virus had a hemagglutination (HA) titer of 210, mean death time (MDT) of 118 hours, and intracerebral pathogenicity index (ICPI) of 0.05. None of these were significantly different from the parental Newcastle disease virus (NDV) LaSota strain (p > 0.05). Vaccination of white leghorn chickens at one day of age with 106 EID50 rNDV-IBV-T/B provided 90% protection against virulent IBV M41 challenge at three weeks of age, which was significantly higher than the protection of the control group vaccinated with phosphate-buffered saline (PBS) (p < 0.05). The ciliostasis scores of rNDV-IBV-T/B-vaccinated and LaSota-vaccinated groups were 4.2 and 37.6, respectively, which indicated that rNDV-IBV-T/B vaccination reduced the pathogenicity of IBV toward the trachea. Furthermore, real-time RT-PCR assay showed that the rNDV-IBV-T/B vaccination resulted in low levels of viral load (647.80 ± 49.65 RNA copies) in the trachea four days post-challenge, which is significantly lower than groups vaccinated with PBS (8591.25 ± 311.10 RNA copies) or LaSota (7742.60 ± 298.50 RNA copies) (p < 0.05). Meanwhile, the same dose of rNDV-IBV-T/B vaccination provided complete protection against velogenic NDV F48E9 challenge. These results demonstrate that the rNDV-IBV-T/B strain is a promising vaccine candidate to control both IB and ND simultaneously. Furthermore, epitope-based live vector vaccines provide an alternative strategy for the development of cost-effective and, broadly, cross-protective vaccines.
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