Das chronische myofasziale Schmerzsyndrom ist eine häufige Ursache von Schmerzen des Bewegungsapparats und mit einem Defizit absteigender hemmender Leitungsbahnen verbunden. Eine Möglichkeit, die intrakortikalen hemmenden Leitungsbahnen zu stimulieren, ist die repetitive transkranielle Magnet-Stimulation (rTMS).
Chronische Beckenschmerzen sind kontinuierlich oder wiederholt auftretende Schmerzen im Bereich des Beckens oder Unterbauches, die über 6 Monate oder länger persistieren. Vermutlich sind rund 4 % der Bevölkerung betroffen. Häufige Ursache ist die Endometriose, für die bisher nur Therapiemethoden mit ungünstigem Nutzen-Risiko-Profil zur Verfügung stehen. Experimentelle Studien zur Therapie der Endometriose weisen darauf hin, dass auch Melatonin die Aktivität neurotropher Faktoren verringern kann, insbesondere vom Brain derived neurotrophic Factor (BDNF), der eine entscheidende Rolle bei der Schmerzentstehung spielt.
Abstract Innovative skin dressings are needed for an effective skin wound treatment. Here in, it was used spongin-like collagen (SC) from marine sponge Chondrilla caribensis as a promising biomaterial, sodium alginate as matrix and, 3D printing technique to manufacture skin dressings in three concentrations (C1, C2, C3). The physicochemical, morphological and biological (in vitro) results were investigated. The results demonstrated that C2 had a higher mass loss, while C3 had a higher pH in experimental periods. Also, a higher porosity was observed for C1 and C2 skin dressings, with a higher swelling ratio for C2. For FTIR it was found peaks of Amide A, -CH2, -COOH and C-O-C, characteristics of collagen. Moreover, the macroscopic image demonstrated a skin dressing with rough surface and grayish color that is naturally observed in Chondrilla caribensis. For SEM analysis the presence of pores could be observed for all skin dressings, with fibers disposed in layers. In the in vitro analyses, the viability of HFF-1 and L929 cell lines were above 70% compared to control group, while for proliferation could be observed a decreased, that could be explained by the excess of stimulus of materials on cells. Furthermore, in cell adhesion analysis, could be observed that both cell lines adhered to all skin dressings in the experimental periods. The results demonstrated that 3D printed skin dressings at different concentrations were able to stimulated cells and were not cytotoxic. Moreover, the findings highlights the potential of SC associated to 3D printing technique as a suitable skin dressing for tissue engineering applications.
The objective was to conduct a literature review of the main rabdomiossrcoma features to better elucidate the scientific community. The Rhabdomyosarcoma is a malignant neoplasm of soft tissues, predominantly found in children, can affect various parts of the body, but is most commonly found in the head and neck. Their clinical presentation is difficult to diagnose, leading to discovery and delayed treatment. Its subtypes vary according to standard histopathological and each leads to a form of treatment, diagnosis and prognosis different. The earlier finding their better prognosis for patients. UNITERMS: Cancer; Rhabdomyosarcoma; Genetics.
This study aimed to compare different protocols (Protocol 1: P1; Protocol 2: P2; Protocol 3: P3; Protocol 4: P4) for the extraction of spongin-like collagen (SC) from marine sponges. The SEM micrographs demonstrated a fibrillar structure for the extracts from Chondrilla caribensis and the nodular/particulate aggregates for Aplysina fulva. FTIR showed for all samples peaks similar to collagen for both species. For C. caribensis, the extracts obtained using P2, P3, and P4 protocols presented higher values of extraction yield, TPQ, and GAGs. P2 and P4 showed higher values of SC concentration and for antioxidant analysis. For A. fulva, P2, P3, and P4 provided a higher extraction yield besides an increase in the antioxidant assay. For both species, no difference was observed for Col quantification and TPQ analysis; also, higher values of GAGs were found using P2 and P4. Fibroblast proliferation observed for C. caribensis was lower for P1 on day 1 and for P2 and P3 on day 3 (for 50%) compared to the control group. There was a significant reduction in fibroblast cell proliferation for all A. fulva extracts evaluated. It can be concluded that protocols P2 and P4 were more efficient for extracting SC from C. caribensis.
Skin wounds are extremely frequent injuries related to many etiologies. They are a burden on healthcare systems worldwide. Skin dressings are the most popular therapy, and collagen is the most commonly used biomaterial, although new sources of collagen have been studied, especially spongin-like from marine sponges (SPG), as a promising source due to a similar composition to vertebrates and the ability to function as a cell-matrix adhesion framework. Despite evidence showing the positive effects of SPG for tissue healing, the effects of skin dressings manufactured are still limited. In this context, this study aimed at investigating the effects of collagen skin dressings in an experimental model of skin wounds in rats. For this purpose, SEM, FTIR, cell viability, morphological and morphometric aspects, collagen deposition, and immunostaining of TGF-β and FGF were evaluated. The results demonstrated micro- and macropores on the rough surface, peak characteristics of collagen, and no cytotoxicity for the skin dressing. Also, the control group (CG) after 5 and 10 days exhibited an intense inflammatory process and the presence of granulation tissue, while the treated group (TG) exhibited re-epithelialization after 10 days. The evaluation of granulation tissue and neoepithelial length had an intragroup statistical difference (p = 0.0216) and no intergroup difference. Birefringence demonstrated an organized mesh arranged in a network pattern, presenting type I and type III collagen fibers in all groups. Moreover, in the morphometric evaluation, there were no statistical differences in intergroups or time points for the different types of collagen evaluated. In conclusion, these findings may indicate that the dressing has not exacerbated the inflammatory process and may allow faster healing. However, further studies using a critical wound healing injury model should be used, associated with longer experimental periods of evaluation, to further investigate the effects of these promising therapeutic approaches throughout the skin repair process.
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