Amanda de Souza

Universidade Federal de São Paulo

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Ana Cláudia Muniz Rennó

Universidade Federal de São Paulo

Karolyne dos Santos Jorge Sousa

Universidade Federal de São Paulo

Renata Neves Granito

Universidade Federal de São Paulo

Matheus de Almeida Cruz

Universidade Federal de São Paulo

Gustavo Oliva Amaral

Universidade Federal de São Paulo

Júlia Risso Parisi

Universidade Metropolitana de Santos

Daniel Araki Ribeiro

Universidade Federal de São Paulo

Cintia Cristina Santi Martignago

Universidade Federal de São Paulo

Giovanna do Espirito Santo

Universidade Federal de São Paulo

Lívia Assis

Universidade Federal de São Paulo

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Universidade Federal de São Paulo

Universidade de São Paulo

Universidade Federal de São Carlos

Universidade do Estado do Pará

Universidade Federal do Rio Grande do Sul

Hospital de Clínicas de Porto Alegre

Instituto de Saúde

Universidade Federal do Rio Grande

Universidade Metropolitana de Santos

Universidade Brasil

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Avaliação citotóxica de Amoxilina e Clavulanato de Potássio em mexilhões <i>Perna perna</i>

Amanda de Souza

Compostos farmacêuticos são identificados em matrizes ambientais em ordens de grandeza de ng.L -1 a µg.L -1 .Dentre os fármacos, os antibióticos têm recebido atenção especial devido aos problemas que podem causar à biota aquática.O objetivo do presente estudo foi avaliar a citotoxicidade de Amoxicilina e Clavulanato de Potássio isolados e em associação para mexilhões Perna perna utilizando o ensaio do tempo de retenção do corante vermelho neutro, que avalia a estabilidade da membrana lisossômica de hemócitos dos organismos teste.A Amoxicilina causou citotoxicidade aos mexilhões nas concentrações de: CEO: 1 ng.L -1 , CI 25-24h : 0,44 ng.L -1 , CI 25-48h : 1,19 ng.L -1 e CI 25-72h : 0,85 ng.L -1 , o Clavulanato de Potássio foi citotóxico nas concentrações de: 10 ng.L -1 em 24h e 50 ng.L -1 e 100 ng.L -1 em 48h e 72h.Os valores de concentração inibitória foram de CI 25-24h : 3,11 ng.L -1 , CI 25-48h : 3,45 ng.L -1 e CI 25-72h : 3,43 ng.L -1 .No ensaio realizado com a associação dos fármacos todas as concentrações foram citotóxicas aos mexilhões em 48h e em 72h apenas 40 ng.L -1 de Amoxicilina + 10 ng.L -1 de Clavulanato de

10.11606/d.85.2016.tde-23052016-085355

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3D Printed Scaffolds Manufactured with Biosilica from Marine Sponges for Bone Healing in a Cranial Defect in Rats

Marine Biotechnology (2023)

Karolyne dos Santos Jorge Sousa Júlia Risso Parisi Amanda de Souza Matheus de Almeida Cruz Rogério Erbereli

3d printed

Viability assay

Immunostaining

10.1007/s10126-023-10202-x

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OS EFEITOS DA CARBOXITERAPIA NAS ATROFIAS CUTÂNEAS ALBAS LOCALIZADAS NA REGIAO GLÚTEA DE MULHERES

Revista CPAQV - Centro de Pesquisas Avançadas em Qualidade de Vida (2020)

Tereza Cristina dos Reis Ferreira Amanda de Souza Patrícia Damasceno da Silva Amanda Jordana Silva Souza Luane Vanzeler Monteiro

Estria é uma atrofia tegumentar adquirida que surge quando as fibras elásticas e colágenas (responsáveis pela firmeza da pele) se rompem e formam “cicatrizes”. As estrias ocorrem mais em mulheres, podendo ser discretas ou exuberantes.quando há destruição de fibras elásticas e colágenas, que são responsáveis pela sustentação e elasticidade da pele. As estrias podem não ser causadas por doenças subjacentes. Algumas causas comuns são gravidez, ganho de peso na obesidade, estirões de crescimento em adolescentes, efeitos colaterais de medicamentos ou rápido aumento do músculo devido a musculação. A terapia de dióxido de carbono (CO2) refere-se à administração transcutânea e subcutânea de CO2 para fins terapêuticos. Caracteriza-se pelo uso terapêutico do gás carbônico medicinal com 99,9% de pureza administrado de forma subcutânea (percutânea) tendo como objetivo uma vasodilatação periférica e melhora da oxigenação tecidual, O objetivo deste estudo foi verificar os efeitos da carboxiterapia nas estrias albas localizadas na região glútea em mulheres jovens. Foram realizadas 10 aplicações de carboxiterapia em ambos os glúteos de cada paciente, sendo feitas avaliações e reavaliações nas pacientes para coleta de dados, sendo adotado um nível de confiança de 5% e 7 graus de liberdade, para este caso o valor tabelado foi t7;0,05 =1,90, para o teste unicaudal. Os resultados deste estudo , sugerem que a carboxiterapia é um recurso que trouxe resultados estatisticamente significantes a respeito da satisfação corporal das pacientes, colaborando com aspecto social e psicológico das mesmas, assim como uma melhor auto-estima com o decorrer das aplicações.

10.36692/v12n3-49

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Cytotoxicity Evaluation of Amoxicillin and Potassium Clavulanate in Perna perna Mussels

Ecotoxicology and Environmental contamination (2016)

Amanda de Souza Beatriz Barbosa Moreno João Emanoel De Almeida Sizue Ota Rogero Camilo Dias Seabra Pereira

Pharmaceutical compounds are identified in environmental matrices in the order of magnitude of ng L-1 to Î¼g L-1. Among the drugs, antibiotics have been receiving special attention due to the problems caused to aquatic biota. The aim of this study was to evaluate the cytotoxicity of Amoxicillin and Potassium Clavulanate, in isolated and associated forms, to marine mussels Perna perna through neutral red retention time assay (NRRT), which assesses the stability of lisossomal membrane of test-organisms hemocytes. Amoxicillin caused cytotoxicity to the mussels in concentrations of OEC= 1 ng L-1, IC25-24h= 0.44 ng L-1, IC25-48h= 1.19 ng L-1 and IC25-72h= 0.85 ng L-1, Potassium Clavulanate was cytotoxic at concentrations of OEC= 10 ngL-1 in 24h; 50 ng L-1 and 100 ng L-1 at 48h and 72h. The inhibitory concentration values (IC25) were: 3.11 ng L-1, 3.45 ng L-1 and 3.43 ng L-1 at 24h, 48h and 72h respectively. In the test conducted with the combination of drugs, all concentrations were cytotoxic to mussels in 48h. In 72h only 40 ng L-1 Amoxicillin + 10 ng L-1 Potassium Clavulanate and 200 ng L-1 Amoxicillin + 50 ng L-1 Potassium Clavulanate were cytotoxic. The IC25 for Amoxicillin was 1.67 ng L-1 in 48h and 1.36 ng L-1 in 72h. For Potassium Clavulanate was 0.42 ng L-1 in 48h and in 72h was 0.34 ng L-1.

Potassium dichromate

Perna perna

10.5132/eec.2016.01.04

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3D‐printed hydroxyapatite scaffolds for bone tissue engineering: A systematic review in experimental animal studies

Journal of Biomedical Materials Research Part B Applied Biomaterials (2022)

Ingrid Regina Avanzi Júlia Risso Parisi Amanda de Souza Matheus de Almeida Cruz Cintia Cristina Santi Martignago

Abstract The use of 3D‐printed hydroxyapatite (HA) scaffolds for stimulating bone healing has been increasing over the years. Although all the promising effects of these scaffolds, there are still few studies and limited understanding of their interaction with bone tissue and their effects on the process of fracture healing. In this context, this study aimed to perform a systematic literature review examining the effects of different 3D‐printed HA scaffolds in bone healing. The search was made according to the preferred reporting items for systematic reviews and meta‐analysis (PRISMA) orientations and Medical Subject Headings (MeSH) descriptors “3D printing,” “bone,” “HA,” “repair,” and “in vivo.” Thirty‐six articles were retrieved from PubMed and Scopus databases. After eligibility analyses, 20 papers were included (covering the period of 2016 and 2021). Results demonstrated that all the studies included in this review showed positive outcomes, indicating the efficacy of scaffolds treated groups in the in vivo experiments for promoting bone healing in different animal models. In conclusion, 3D‐printed HA scaffolds are excellent candidates as bone grafts due to their bioactivity and good bone interaction.

3d printed

Animal model

10.1002/jbm.b.35134

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Injectable hydrogels for treating skin injuries in diabetic animal models: a systematic review

Journal of Diabetes & Metabolic Disorders (2024)

Gustavo Oliva Amaral Giovanna do Espirito Santo Ingrid Regina Avanzi Júlia Risso Parisi Amanda de Souza

Biocompatibility

10.1007/s40200-024-01510-7

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In vivo effects of biosilica and spongin-like collagen scaffolds on the healing process in osteoporotic rats

Research Square (Research Square) (2024)

Matheus de Almeida Cruz Karolyne Santos Jorge Sousa Ingrid Regina Avanzi Amanda de Souza Cintia Santi Martignago

Abstract Due to bioactive properties, introducing spongin-like collagen (SPG) into the biosilica (BS) extracted from marine sponges, would present an enhanced biological material for improving osteoporotic fracture healing by increasing bone formation rate. Our aim was to characterize the morphology of the BS/SPG scaffolds by scanning electron microscopy (SEM), the chemical bonds of the material by Fourier transform infrared spectroscopy (FTIR) and evaluate the orthotopic in vivo response of BS/SPG scaffolds in tibial defects of osteoporotic fractures in rats (histology, histomorphometry and immunohistochemistry) in 2 experimental periods (15 and 30 days). SEM showed that scaffolds were porous, showing the spicules of BS and fibrous aspect of SPG. FTIR showed characteristic peaks of BS and SPG. For the in vivo studies, after 30 days BS and BS/SPG showed a higher amount of newly formed bone compared to the first experimental period, observed both in the periphery and in the central region of the bone defect. For histomorphometry, BS/SPG presented higher %BV/TV compared to the other experimental groups. After 15 days, BS presented higher volumes of collagen type I. After 30 days, all groups demonstrated higher volumes of collagen type III compared to volumes at 15 days. After 30 days, BS/SPG presented higher immunostaining of osteoprotegerin compared to the other experimental groups at the same experimental period. The results showed that BS and BS/SPG scaffolds were able of improving bone healing. Future researches should focus on the effects of BS/SPG on longer periods in vivo studies.

10.21203/rs.3.rs-4283707/v1

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Development and comparison of two 3D printed scaffolds of biosilica from marine sponges for bone tissue engineering

Research Square (Research Square) (2024)

Giovanna do Espirito Santo Amanda de Souza Gustavo Oliva Amaral Amanda Sardeli Alqualo Karolyne dos Santos Jorge Sousa

Abstract This study compared the physicochemical characteristics and biological effects of two 3D printed biosilica (BS) scaffolds (grid and gyroid). Methods included Scanning Electron Microscopy (SEM), Micro-Computed Tomography (Micro-CT), Mass Loss and pH Assessment, Fourier-Transform Infrared Spectroscopy (FTIR), and Energy-Dispersive X-ray Spectroscopy (EDS). The mechanical evaluation involved a Compression Test, and in vitro tests used cell adhesion assays with osteoblastic (MC3T3-E1) and fibroblastic (L929) cell lines. SEM showed BS spicules in both models on day 0, with signs of degradation along the experimental periods of immersion, forming a homogeneous network with the interaction with alginate. Micro-CT revealed rough surfaces in both models, with the gyroid model being more homogeneous and porous, with larger pores compared to the grid model. The gyroid model demonstrated higher values in the compression test and a decrease in pH on the first day and no differences for both models on days 3, 7, and 14. The mass loss was higher in the gyroid model by day 21. FTIR tests showed characteristic peaks for ALG and BS. EDS detected silica (Si), chlorine (Cl), calcium (Ca), carbon (C), and oxygen (O). In cell adhesion assays, both models supported adhesion and proliferation of L929 and MC3T3-E1 cells, with the gyroid model showing better cell elongation and morphology. Overall, the gyroid model demonstrated superior physicochemical properties, greater mechanical strength, and enhanced biological performance compared to the grid model, making it more promising for tissue engineering applications.

Gyroid

Simulated body fluid

10.21203/rs.3.rs-4914115/v1

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3D printed scaffolds of biosilica and spongin from marine sponges: Analysis of genotoxicity and cytotoxicity for bone tissue repair

Research Square (Research Square) (2024)

Karolyne dos Santos Jorge Sousa Amanda de Souza Matheus de Almeida Cruz Lindiane Eloísa de Lima Giovanna do Espirito Santo

Abstract Biosilica (BS) and spongin (SPG) from marine sponges are highlighted for their potential to promote bone regeneration. Moreover, additive manufacturing, specifically 3D printing, is introduced as a technology for producing bone grafts with optimized interconnected porous structures, allowing for better cell attachment, proliferation, and differentiation. Thus, the aims of this study were to characterize the BS and BS/SPG 3D printed scaffolds and to evaluate the biological effects in vitro . The physicochemical characteristics of BS and BS/SPG 3D printed scaffolds were analyzed by SEM, FTIR, porosity, evaluation of mass loss, and pH measurement. For in vitro analysis, the cellular viability of the MC3T3-E1 cell lineage was assessed using the AlamarBlue® assay and SEM, while genotoxicity was evaluated through the micronucleus assay. SEM analysis revealed distinct features: the presence of spicules in BS, the fibrillar structure of SPG, and material degradation over the immersion period. FTIR indicated peaks corresponding to silicon oxide in BS samples and carbon oxide and amine in SPG samples. BS-SPG scaffolds exhibited higher porosity, while BS scaffolds displayed greater mass loss. pH measurements indicated a significant decrease induced by BS, which was mitigated by SPG over the experimental periods. In vitro studies demonstrated the biocompatibility and non-cytotoxicity of scaffold extracts. The micronucleus (MN) test further confirmed the absence of cytotoxicity in the samples. These findings suggest that 3D printed BS and BS/SPG scaffolds may possess desirable morphological and physicochemical properties, indicating in vitro biocompatibility.

10.21203/rs.3.rs-3940069/v1

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Flounder fish (Paralichthys sp.) collagen a new tissue regeneration: genotoxicity, cytotoxicity and physical–chemistry characterization

Bioprocess and Biosystems Engineering (2023)

Karolyne dos Santos Jorge Sousa Amanda de Souza Lindiane Eloísa de Lima Rogério Erbereli Jonas de Araújo Silva

Comet Assay

Viability assay

10.1007/s00449-023-02884-3

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Citations (2)