David López-González

Universidade de Vigo

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Adela M. Sánchez‐Moreiras

Universidade de Vigo

Fabrizio Araniti

University of Milan

Manuel J. Reigosa

Universidade de Vigo

Mercedes Verdeguer

Universitat Politècnica de València

Marta Teijeira

Universidade de Vigo

Elisa Graña

Universidade de Vigo

SÁ

Sara Álvarez‐Rodríguez

Universidade de Vigo

Jesús Simal‐Gándara

Universidade de Vigo

Miguel A. Prieto

Universidade de Vigo

Muhammad Iftikhar Hussain

Universidade de Vigo

Cooperative Institutions

Universidade de Vigo

Universitat Politècnica de València

Universidade de Santiago de Compostela

University of Basilicata

University of Calabria

Faculty (United Kingdom)

Università di Camerino

Servicio Gallego de Salud

University of Milan

University of Reggio Calabria

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Investigating the phytotoxic potential of Carlina acaulis essential oil against the weed Bidens pilosa through a physiological and metabolomic approach

Industrial Crops and Products (2023)

Sara Álvarez‐Rodríguez Eleonora Spinozzi Adela M. Sánchez‐Moreiras David López-González Marta Ferrati

Essential oils (EOs) are widely studied as possible candidates for new eco-friendly herbicides for weed management due to their phytotoxicity. In this study we tested the phytotoxic potential of the EO obtained from the roots of Carlina acaulis L. (Apiaceae) against the weed Bidens pilosa L. This EO, containing 98% of the polyacetylene carlina oxide, showed strong phytotoxic effects on the plant metabolism, such as leaf necrosis, reduction of relative water content and total leaf area, and an increase in the dry weight/fresh weight ratio, suggesting a water status alteration. The EO also damaged the photosynthetic machinery, as evidenced by the significant reduction of the effective quantum yield of photosystem II (ΦII) and the maximum quantum yield of photosystem II (Fv/Fm). In addition, the non-photochemical quenching (ΦNPQ) significantly increased after spraying with C. acaulis EO. Damage to photosystem II was further demonstrated through the reduction of manganese and calcium concentrations, possibly due to an alteration in the correct functionality of the Mn4Ca cluster of the PSII. Metabolomics analysis revealed an accumulation of branched-chain amino acids, such as isoleucine and valine, which is commonly related to osmotic alterations under drought stress situations and a general reduction in sugar content (fructose, glucose, mannose, among others), suggesting reduction of the photosynthetic efficiency too. Overall, these findings suggest C. acaulis EO as a promising natural product with phytotoxic potential against weeds that deserves further investigation.

Bidens pilosa

Phytotoxicity

10.1016/j.indcrop.2023.117149

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Citations (10)

Phytotoxic Activity of the Natural Compound Norharmane on Crops, Weeds and Model Plants

Plants (2020)

David López-González David Ledo Luz Cabeiras-Freijanes Mercedes Verdeguer Manuel J. Reigosa

Norharmane is a secondary metabolite that appears in different species of land plants. In this paper, we investigated for the first time the specificity of norharmane through germination and growth tests on some crops as Zea mays L. (maize), Triticum aestivum L. (wheat), Oryza sativa L. (rice) and Lactuca sativa L. (lettuce) and weeds as Amaranthus retroflexus L. (amaranth), Echinochloa crus-galli L. (barnyard grass), Plantago lanceolata L. (ribwort), Portulaca oleracea L. (common purslane) and Avena fatua L. (wild oat), and its phytotoxic capacity on the metabolism of adult Arabidopsis thaliana L. (thale cress) by measuring chlorophyll a fluorescence, pigment content, total proteins, osmotic potential and morphological analysis. Norharmane had an inhibitory effect on the germination of A. fatua and P. lanceolata, and the growth of P. oleracea, E. crus-galli and A. retroflexus. On adult A. thaliana plants, the compound was more effective to watering, leading to water stress that compromised the growth of the plants and ultimately affected the photosynthetic apparatus. Therefore, this research shows that norharmane not only affects seedlings’ metabolism, but also damages the metabolism of adult plants and can be a potential model for a future bioherbicide given its specificity.

Avena fatua

Bioherbicide

Allelopathy

Avena

Phytotoxicity

Galium

10.3390/plants9101328

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Bio-Products Obtained from Broccoli and Cabbage Wastes Are Proposed as Functional Food Ingredients and Bioherbicides for Sustainable Weed Management

Sustainability (2025)

Claudia Bas-Bellver Nieves Melero-Carnero David López-González Cristina Barrera Mercedes Verdeguer

Developing processes that contribute to the valorization of vegetable wastes is of great importance since these residues are characterized by being of high quality, having a huge potential for valorization. However, upcycling vegetables residues and defining specific applications for the value-added products obtained might be a challenge, and they should be tackled by means of different and complementary innovations. In the present study, broccoli and white cabbage discards were transformed into powdered products by means of selected techniques and conditions, which have been explored for applications in the agri-food sector. The obtained brassica powders were rich in bioactive compounds such as phenolics and isothiocyanates. Their antioxidant properties in response to in vitro digestion were evaluated to assess the potential of the products as functional food ingredients. On the other hand, brassica powders were tested as bioherbicides. For that purpose, inhibition tests on weed germination and growth of weeds from agricultural soil seedbank and selected species (Lolium rigidum, Papaver rhoeas, Portulaca oleracea, and Echicnochloa crus-galli) were performed under controlled greenhouse conditions. In vitro simulated digestion studies demonstrated that bioactive constituents of powders were progressively released during digestion, and consequently, a part of them could be finally absorbed and, thus, provide their beneficial effect. Brassica bioproducts significantly reduced the germination of weeds from the agricultural soil seedbank, and the selected weed species tested, namely L. rigidum, P. rhoeas, P. oleracea, and E. crus-galli. Powders also showed a negative effect on the root weight and length of dicotyledonous weeds from the soil seedbank and L. rigidum, whereas a stimulatory effect was observed on the spring–summer species, E. crus-galli and P. oleracea. The results of this work contribute to extending the range of applications for brassica industrialization wastes.

10.3390/su17010282

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Trans-cinnamaldehyde-related overproduction of benzoic acid and oxidative stress on Arabidopsis thaliana

Frontiers in Plant Science (2023)

David López-González Yolanda Ferradás Fabrizio Araniti Elisa Graña José M. Hermida‐Ramón

Trans-cinnamaldehyde is a specialised metabolite that naturally occurs in plants of the Lauraceae family. This study focused on the phytotoxic effects of this compound on the morphology and metabolism of Arabidopsis thaliana seedlings.To evaluate the phytotoxicity of trans-cinnamaldehyde, a dose-response curve was first performed for the root growth process in order to calculate the reference inhibitory concentrations IC50 and IC80 (trans-cinnamaldehyde concentrations inducing a 50% and 80% inhibition, respectively). Subsequently, the structure and ultrastructure of the roots treated with the compound were analysed by light and electron microscopy. Based on these results, the following assays were carried out to in depth study the possible mode of action of the compound: antiauxinic PCIB reversion bioassay, determination of mitochondrial membrane potential, ROS detection, lipid peroxidation content, hormone quantification, in silico studies and gene expression of ALDH enzymes.Trans-cinnamaldehyde IC50 and IC80 values were as low as 46 and 87 μM, reducing the root growth and inducing the occurrence of adventitious roots. At the ultrastructural level, the compound caused alterations to the mitochondria, which were confirmed by detection of the mitochondrial membrane potential. The morphology observed after the treatment (i.e., appearance of adventitious roots) suggested a possible hormonal mismatch at the auxin level, which was confirmed after PCIB bioassay and hormone quantification by GC-MS. The addition of the compound caused an increase in benzoic, salicylic and indoleacetic acid content, which was related to the increased gene expression of the aldehyde dehydrogenase enzymes that can drive the conversion of trans-cinnamaldehyde to cinnamic acid. Also, an increase of ROS was also observed in treated roots. The enzyme-compound interaction was shown to be stable over time by docking and molecular dynamics assays.The aldehyde dehydrogenases could drive the conversion of trans-cinnamaldehyde to cinnamic acid, increasing the levels of benzoic, salicylic and indoleacetic acids and causing the oxidative stress symptoms observed in the treated seedlings. This would result into growth and development inhibition of the trans-cinnamaldehyde-treated seedlings and ultimately in their programmed-cell-death.

10.3389/fpls.2023.1157309

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Citations (7)

The plant secondary metabolite citral alters water status and prevents seed formation in Arabidopsis thaliana

Plant Biology (2015)

Elisa Graña Carla Díaz‐Tielas David López-González Ana Martínez-Peñalver Manuel J. Reigosa

Based on previous results, which showed that the secondary metabolite citral causes disturbances to plant water status, the present study is focused on demonstrating and detailing these effects on the water-related parameters of Arabidopsis thaliana adult plants, and their impact on plant fitness. Clear evidence of effects on water status and fitness were observed: plants treated with 1200 and 2400 μm citral showed decreased RWC, reduced Ψs , increased Ψw and reduced stomatal opening, even 7 days after the beginning of the experiment. Plant protection signals, such as leaf rolling or increased anthocyanin content, were also detected in these plants. In contrast, 14 days after beginning the treatment, treated plants showed signs of citral-related damage. Moreover, the reproductive success of treated plants was critically compromised, with prematurely withered flowers and no silique or seed development. This effect of citral on fitness of adult plants suggests a promising application of this natural compound in weed management by reducing the weed seed bank in the soil.

Citral

Silique

Secondary metabolite

10.1111/plb.12418

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Citations (15)

Short-Term Effects of Trans-Cinnamic Acid on the Metabolism of Zea mays L. Roots

Plants (2023)

David López-González Leonardo Bruno Carla Díaz‐Tielas Antonio Lupini Meriem Miyassa Aci

-Cinnamic acid is a phenolic compound widely studied in plant metabolism due to its importance in regulating different plant processes. Previous studies on maize plants showed that this compound could affect plant growth and causes metabolic changes in the leaves when applied. However, its effects on root metabolism are not well known. This study analyses the short-term effect of

Cinnamic acid

Plant Physiology

10.3390/plants12010189

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Citations (2)

Soil organic matter quality in an olive orchard differently managed for 21 years: Insights into its distribution through soil aggregates and depth

Agriculture Ecosystems & Environment (2024)

Rosangela Addesso Fabrizio Araniti Andrea Bloise Alba N. Mininni Bartolomeo Dichio

Orchard

Soil Quality

10.1016/j.agee.2024.109388

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Ultrastructural and hormonal changes related to harmaline-induced treatment in Arabidopsis thaliana (L.) Heynh. root meristem

Plant Physiology and Biochemistry (2022)

Sara Álvarez‐Rodríguez David López-González Manuel J. Reigosa Fabrizio Araniti Adela M. Sánchez‐Moreiras

Harmaline is an indole alkaloid with demonstrated phytotoxicity and recognized pharmacological applications. However, no information is available concerning its mode of action on plant metabolism. Therefore, the present work evaluated bioherbicide mode of action of harmaline on plant metabolism of Arabidopsis thaliana (L.) Heynh. Harmaline induced a strong inhibitory activity on root growth of treated seedlings, reaching IC50 and IC80 values of 14 and 29 μM, respectively. Treated roots were shorter and thicker than control and were characterized by a shorter root meristem size and an increase of root hairs production. Harmaline induced ultrastructural changes such as increment of cell wall thickness, higher density and condensation of mitochondria and vacuolization, appearance of cell wall deposits, increment of Golgi secretory activity and higher percentage of aberrant nuclei. The ethylene inhibitor AgNO3 reversed high root hair appearance and increment of root thickness, and pTCSn::GFP transgenic line showed fluorescence cytokinin signal in stele zone after harmaline treatment that was absent in control, whereas the auxin signal in the transgenic line DR5 was significantly reduced by the treatment. All these results suggest that the mode of action of harmaline could be involving auxin, ethylene and cytokinin synergic/antagonistic action.

Harmaline

Root hair

10.1016/j.plaphy.2022.03.022

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Citations (6)