Introdução: Durante a superovulação ovariana, uma "corte" de oócitos é recrutada, sendo determinado seu estágio de maturidade, imediatamente antes da microinjeção.Pouco se sab e a respeito da viabilidade dos oócitos imaturos, de seu potencial de fe cundação e de sua importância nos resultados da injeção intracitoplasmática de espermatozóides (lCSI).Nosso objetivo fo i estudar, nos procedimentos de ICSI, a fr eqüência de oócitos imaturos humanos, a probabilidade de maturação e de fe rtilização "in vitro".Analisou-se prospectivamente o número de oócitos em prófa se (PI), metáfa se I (MI) e metáfa se 11 (M1I), estabelecendo correlações entre as taxas de maturação e fe rtilização "in vitro" e o impacto sobre os resultados de gra videz.
Summary The aim of the present study was to determine the chance of pregnancy and the risk of multiple pregnancies taking into account the number and quality of transferred embryos in patients >36 years old or ≤36 years old. For this study, 1497 patients undergoing intra-cytoplasmic sperm injection (ICSI) cycles in a private assisted reproduction centre were split into groups according to the number and quality of the transferred embryos on the third or fifth day of development. The pregnancy rate and multiple pregnancy rate were compared between the embryo quality groups in patients <36 years old or ≥36 years old. In patients <36 years old, for the day 3 embryo transfer, no significant difference was noted in the pregnancy rate when the groups were compared. However the multiple pregnancy rate was increased by the transfer of an extra low-quality embryo (17.1 versus 28.2%, P = 0.020). For day 5 embryo transfer, the transfer of an extra blastocyst significantly increased the pregnancy rate (36.0 versus 42.4%, P < 0.001) and the multiple pregnancy rate (4.4 versus 16.9%, P < 0.001). In older patients, no significant difference was noted in the pregnancy rate when the groups were compared. However, when an extra low-quality embryo was transferred, a significantly increased rate of multiple pregnancies was observed for day 3 (18.2 versus 26.4%, P = 0.049) and day 5 embryo transfers (5.2 versus 16.1%, P < 0.001). In conclusion, the transfer of an extra low-quality embryo may increase the risk of a multiple pregnancy. In younger patients, the transfer of an extra low-quality blastocyst may also increase the chance of pregnancy.
Objective: to determine if eating habits, physical activity and BMI can influence assisted reproduction outcomes. Material and Methods: this study analyzed 436 patients undergoing intracytoplasmic sperm injection cycles. Patients answered a questionnaire and regression analysis examined the relationship between lifestyle and BMI with the intracytoplasmic sperm injection cycles outcomes. Results: no influence of lifestyle and obesity was observed on the number of oocytes recovered. Obesity reduced the normal fertilization rate (coefficient [Coef.]: −16.0; p = 0.01) and increased the risk of miscarriage (OR: 14.3; p = 0.03). Physical activity positively affected implantation (Coef.: 9.4; p = 0.009), increased the chance of pregnancy (OR: 1.83; p = 0.013) and tended to decrease the risk of miscarriage (OR: 0.30; p = 0.068). In addition, an inverse correlation was found between physical activity and BMI, and a direct correlation was found between soft-drink consumption and BMI. Conclusions: eating habits, physical activity and obesity could affect clinical outcomes of assisted reproduction.