Chaur‐Tsuen Lo

National Formosa University

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Kou‐Cheng Peng

National Dong Hwa University

Guor‐Cheng Fang

Chongqing University of Technology

Shuying Liu

Jilin Agricultural University

Yuan-Jie Zhuang

Hungkuang University

Chi‐Hua Cheng

National Dong Hwa University

Meng-Hsien Cho

Hungkuang University

Hsueh‐Hui Yang

Hualien Tzu Chi Medical Center

Kai-Hsiang Tsai

Hungkuang University

Chia‐Ann Yang

Tzu Chi University

You-Fu Xiao

Hungkuang University

Cooperative Institutions

National Formosa University

National Dong Hwa University

National Chung Hsing University

Hungkuang University

Tzu Chi University

National University of Formosa

Dayeh University

National Taipei University

National Taiwan University

National Synchrotron Radiation Research Center

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Seasonal Variations (Autumn, Winter, and Spring) of Atmospheric Particulate and Particulate-Bound Mercury Hg(p) at a Suburban/Coastal Area

Environmental Forensics (2010)

Guor‐Cheng Fang Chaur‐Tsuen Lo Chia-Kuan Liu

From September 3, 2009, to March 5, 2010, the dry deposition, total suspended particulate (TSP) matter, coarse and fine particulates, and bound mercury (Hg[p]) in the atmosphere were investigated at Bei-Shi sampling site in central Taiwan. When using projection film as collection medium, the average ambient air particulates for day dry deposition fluxes and TSPs were 162.09 ± 97.69 (μg/(min/cm2)) and 78.71 ± 34.42 (μg/m3), respectively. The average fine particulate matter (PM2.5) and coarse particulate matter (PM2.5–10) were 20.5 ± 6.30 μgm−3 and 11.2 ± 4.44 μg m−3, respectively. Average atmospheric particulates Hg(p) in dry depositions, TSPs, PM2.5−10, and PM2.5were 0.156 ± 0.082 ng/m2*min, 0.07 ± 0.036 ng/m3, 0.011 ± 0.006 ng/m3,and 0.007 ± 0.005, respectively. The average compositions of atmospheric bound Hg Hg(p) in dry depositions, TSPs, PM2.5−10 and PM2.5 were 1.02, 36.8, 4.16 and 2.821, respectively. Also, the highest composition for particulate Hg(p) in TSP was observed in spring (50.74 ng/g), and the highest composition for particulate Hg(p) in PM2.5 occurred in autumn (3.11 ng/g). However, the highest composition for particulate Hg(p) in PM2.5−10 occurred in winter (4.67 ng/g). In what concerns particulate Hg(p) in TSP, the values were all less than those from previous studies (Wang et al., 2006 Wang, Z., Zhang, X., Chen, Z. and Zhang, Y. 2006. Mercury concentrations in size-fractionated airborne particles at urban and suburban sites in Beijing, China. Atmospheric Environment, 40: 2194–2201. [Crossref], [Web of Science ®] , [Google Scholar]). Related to Hg(p) in PM2.5, the values were also all less than those from previous studies (Tsai et al. 2003 Tsai, Y. I., Kuo, S. and Lin, Y. 2003. Temporal characteristics of inhalable mercury and arsenic aerosols in the urban atmosphere in southern Taiwan. Atmospheric Environment, 37: 3401–3411. [Crossref], [Web of Science ®] , [Google Scholar]; Marsik et al. 2007 Marsik, F. J., Keeler, G. J. and Landis, M. S. 2007. The dry-deposition of speciated mercury to the Florida Everglades: Measurements and modeling. Atmospheric Environment, 41: 36–149. [Crossref], [Web of Science ®] , [Google Scholar]). In what concerns Hg(p) in PM2.5−10, the values were all less than those of previous studies (Marsik et al. 2007 Marsik, F. J., Keeler, G. J. and Landis, M. S. 2007. The dry-deposition of speciated mercury to the Florida Everglades: Measurements and modeling. Atmospheric Environment, 41: 36–149. [Crossref], [Web of Science ®] , [Google Scholar]). The data presented in this communication provides information that may be useful in forensic investigations of Hg environmental releases.

Mercury

Deposition

10.1080/15275922.2010.523757

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Citations (10)

Annual ambient atmospheric mercury speciation measurement from Longjing, a rural site in Taiwan

Environmental Geochemistry and Health (2016)

Guor‐Cheng Fang Chaur‐Tsuen Lo Meng-Hsien Cho Yuan-Jie Zhuang Kai-Hsiang Tsai

Mercury

Genetic algorithm

10.1007/s10653-016-9861-x

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Citations (14)

Purification, identification and characterization of Nag2 N-acetylglucosaminidase from Trichoderma virens strain mango

Botanical studies (2022)

Jheng-Hua Huang Feng‐Jin Zeng Jhe-Fu Guo Jian-Yuan Huang Hua‐Chian Lin

N-acetylglucosaminidase (NAGase) could liberate N-acetylglucosamine (GlcNAc) from GlcNAc-containing oligosaccharides. Trichoderma spp. is an important source of chitinase, particularly NAGase for industrial use. nag1 and nag2 genes encoding NAGase, are found in the genome in Trichoderma spp. The deduced Nag1 and Nag2 shares ~ 55% homology in Trichoderma virens. Most studies were focus on Nag1 and nag1 previously.The native NAGase (TvmNAG2) was purified to homogeneity with molecular mass of ~ 68 kDa on SDS-PAGE analysis, and identified as Nag2 by MALDI/MS analysis from an isolate T. virens strain mango. RT-PCR analyses revealed that only nag2 gene was expressed in liquid culture of T. virens, while both of nag1 and nag2 were expressed in T. virens cultured on the plates. TvmNAG2 was thermally stable up to 60 °C for 2 h, and the optimal pH and temperature were 5.0 and 60-65 °C, respectively, using p-nitrophenyl-N-acetyl-β-D-glucosaminide (pNP-NAG) as substrate. The hydrolytic product of colloidal chitin by TvmNAG2 was suggested to be GlcNAc based on TLC analyses. Moreover, TvmNAG2 possesses antifungal activity, inhibiting the mycelium growth of Sclerotium rolfsii. And it was resistant to the proteolysis by papain and trypsin.The native Nag2, TvmNAG2 was purified and identified from T. virens strain mango, as well as enzymatic properties. To our knowledge, it is the first report with the properties of native Trichoderma Nag2.

Chitinase

Trichoderma

Molecular mass

10.1186/s40529-022-00344-x

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Sources of ambient air particulates and Hg(p) pollutants at Freeway, Industrial, Thermal power plant F.I.T. characteristic sites

Environmental Earth Sciences (2016)

Guor‐Cheng Fang Chaur‐Tsuen Lo Yuan-Jie Zhuang Yu-Chen Kuo Meng-Hsien Cho

Mercury

Deposition

Scavenging

10.1007/s12665-015-5057-4

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Citations (8)

Plate assay for fungal enzymes using cellophane membranes

Analytical Biochemistry (2009)

Shuying Liu Pei-Char Feng Chaur‐Tsuen Lo Kou‐Cheng Peng

Cellophane

10.1016/j.ab.2009.09.016

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Citations (11)

Oospore dormancy, germination and survival of Pythium aphanidermatum.

Plant Protection Bulletin (Taipei) (1992)

Y. S. Lin Chaur‐Tsuen Lo C.H. Hwang

Pythium aphanidermatum

Oospore

Source

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Citations (1)

Comparisons of particulate-bound mercury (PBM) compositions in soil and vegetation at a traffic site

Environmental Forensics (2017)

Guor‐Cheng Fang Chaur‐Tsuen Lo Meng-Hsien Cho Yuan-Jie Zhuang Chao-Yang Huang

This investigation analyzes the particulate-bound mercury (PBM) compositions in soil and vegetation at a traffic sampling site in Taichung, Taiwan, during a sampling period from October to December, 2015. A direct mercury analyzer (DMA-80) was used to measure the particulate-bound mercury (PBM). A T-test was conducted to determine the mean differences between the PBM composition in soil and that in vegetation at the site. The results indicate that 1) the mean particulate-bound mercury compositions in soil and vegetation were the lowest in November, when the (mean OR average) wind speed was the highest (4.1 m/s); 2) Particulate-bound mercury compositions (PBM) in both soil and vegetation correlated weakly with temperature, humidity and wind speed; 3) T-test statistical results denoted that the PBM compositions did not significantly differ between soil and vegetation in the three-month sampling period.

Mercury

10.1080/15275922.2017.1408156

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Citations (0)

Physiological races of Fusarium oxysporum f.sp. pisi in Taiwan.

Plant Protection Bulletin (Taipei) (1990)

P. H. Wang Chaur‐Tsuen Lo Y. S. Lin

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Improved Biocontrol Efficacy of Trichoderma harzianum 1295-22 for Foliar Phases of Turf Diseases by Use of Spray Applications

Plant Disease (1997)

Chaur‐Tsuen Lo Eric B. Nelson Gary E. Harman

Trichoderma harzianum strain 1295-22 is an effective biocontrol agent for several fungal diseases. The efficacy of granule and spray applications of strain 1295-22 for control of Pythium root rot, brown patch, and dollar spot of creeping bentgrass was investigated. Spray applications of conidial suspensions (SA) of strain 1295-22 significantly reduced all three diseases of creeping bentgrass turf in both greenhouse and field experiments. Control was greatest when Triton X-100 at 0.1% was added to aqueous spray suspensions. When SA were applied weekly, the biocontrol treatments were equivalent to standard fungicides. Broadcast granule applications (GA) also significantly reduced foliar symptoms of Pythium root rot, dollar spot, and brown patch; turf quality also was enhanced. The populations of Trichoderma spp. in the root zone of a bentgrass putting green treated with SA or GA of strain 1295-22 increased 10- to 100-fold after treatment compared with untreated plots. However, strain 1295-22 was present at high levels on bentgrass leaves only following SA. Collectively, the results suggest that strain 1295-22 possesses both rhizosphere and phylloplane competence. The combination of broadcast applications of granules followed by spray applications of conidia reduced damage from both root and foliar diseases.

Trichoderma harzianum

Pythium ultimum

Pythium

Trichoderma

Root rot

Phyllosphere

10.1094/pdis.1997.81.10.1132

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Citations (70)

Involvement of Pachybasin and Emodin in Self-Regulation of Trichoderma harzianum Mycoparasitic Coiling

Journal of Agricultural and Food Chemistry (2012)

Yi-Ruu Lin Chaur‐Tsuen Lo Shuying Liu Kou‐Cheng Peng

Our aim was to determine the effects of two secondary metabolites secreted by Trichoderma harzianum, pachybasin and emodin, on the mycoparasitic coiling behavior and cAMP content of T. harzianum. The number of T. harzianum coils around Nylon 66 fiber was increased in the presence of R. solani. The number of T. harzianum coils around R. solani hyphae and Nylon 66 fiber were significantly increased in the presence of pachybasin and emodin. The cAMP level in T. harzianum was significantly increased by close contact with R. solani and much higer cAMP level in the presence of exogenous pachybasin and emodin. A cAMP inhibitor diminished the effect of pachybasin and emodin on T. harzianum coiling around Nylon 66 fiber. The results suggest that pachybasin and emodin mediate the increase in the number of Trichoderma mycoparasitic coils via cAMP signaling. This is the first report to suggest that pachybasin and emodin play roles in the biocontrol mechanism of Trichoderma.

Trichoderma harzianum

Trichoderma

10.1021/jf202773y

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Citations (40)