Journal Article Dr. Gottfried’s Reply Get access Eugene L. Gottfried, M.D. Eugene L. Gottfried, M.D. Laboratory of Clinical Hematology The New York Hospital – Cornell Medical Center New York, New York 10021 Search for other works by this author on: Oxford Academic Google Scholar American Journal of Clinical Pathology, Volume 67, Issue 3, 1 March 1977, Pages 313–314, https://doi.org/10.1093/ajcp/67.3.313a Published: 01 March 1977 Article history Received: 29 November 1976 Accepted: 29 November 1976 Published: 01 March 1977
Elevated hemoglobin A2 (Hb A2) levels can be identified conveniently by densitometry after electrophoresis on cellulose acetate strips. Because a recent report questioned the accuracy of this technic, the method was re-evaluated by paired comparison with microcolumn chromatography. Analysis of 100 patient specimens showed high correlation (r = +0.84), but an average Hb A2 concentration 0.7% higher by densitometry than by chromatography (P < 0.001). With upper limits set at 4.5% and 3.8%, respectively, results were divided into “normal” and “high” for each method. Concordant results were obtained in 97 of the 100 cases (82 normal, 15 high). Another densitometer of improved design was used for paired analysis of 50 additional specimens, 25 normal and 25 with β-thalassemia trait. The two groups were well separated by both procedures, arid Hb A2 levels were similar (r = +0.92, P > 0.6). This study demonstrates that it is possible, with carefully controlled technics and properly calibrated instruments, to use electrophoresis with densitometry as a reliable means of identifying abnormal Hb A2 levels.
Use of the International Normalized Ratio (INR) has been recommended as a means of standardizing prothrombin time (PT) results for management of oral anticoagulant therapy. During the evaluation of a new lot of thromboplastin reagent, however, INR values were obtained that were inconsistent with results obtained with the prior lot of reagent from the same manufacturer. A local normalized ratio (LNR) was substituted for the INR for the new reagent, based on a calculated local sensitivity index (LSI). Validation of the LSI was performed at the three hospitals in the medical community by testing an identical panel of aliquots from 64 plasmas obtained from patients who were chronically receiving oral anticoagulants. Each test result was classified according to the INR value as low, low therapeutic, high therapeutic, or high. Local normalized ratio values obtained at one hospital in the community were in reasonable agreement with INR determinations in the other two hospital laboratories. Classification mismatches occurred using the INR, however, in 84 of 280 (30%) of the paired samples. Thus, the inability to generate consistent INR values within a local medical community raises serious concern about the reliability of the INR concept in its current form.
PIPERAZINE, available for oral use as the citrate,¶ has been found effective against pinworm and ascaris infections.1 2 3 Its structural formula is as follows: Widespread experience with this compound has disclosed virtually no significant toxic manifestations at recommended doses of 50 to 75 mg. of the hexahydrate per kilogram of body weight per day, and only mild diarrhea and occasional urticaria are listed in a standard textbook of pharmacology.4 Neurologic findings have been reported in England by White and Standen5 and by Sims,6 and in this country by Howie,7 in patients taking up to three times the recommended dosage. An unusual . . .
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Biochemistry of Plasmalogens. III. Concentrations in Tissues of the Rat as a Function of Age*Eugene L. Gottfried, Maurice M. Rapport, and Mrs. Phyllis RosenCite this: Biochemistry 1963, 2, 4, 646–648Publication Date (Print):July 1, 1963Publication History Published online1 May 2002Published inissue 1 July 1963https://pubs.acs.org/doi/10.1021/bi00904a004https://doi.org/10.1021/bi00904a004research-articleACS PublicationsRequest reuse permissionsArticle Views41Altmetric-Citations17LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The clinical value of a laboratory test depends on more than just a statistically significant difference between two test groups. Grimm et al1may have overemphasized the prognostic importance of the white blood cell count for coronary heart disease. The unwary may be misled into believing that a blood cell count can actually identify persons at risk. When the test groups are large, even minor differences may be statistically significant. For example, in one study of about 1,300 healthy subjects,2there was a small but highly significant sex difference in the platelet count (P<.0001). Even so, there are better ways than a platelet count to separate the girls from the boys!
Journal Article The Diagnostic Significance of a Prolonged Erythrocytic Glycerol Lysis Time (GLT50) Get access Anthony Posteraro, Jr., M.D., Anthony Posteraro, Jr., M.D. From the Departments of Medicine and Pathology, The New York Hospital–Cornell Medical Center, New York, New York Search for other works by this author on: Oxford Academic Google Scholar Eugene L Gottfried, M.D. Eugene L Gottfried, M.D. From the Departments of Medicine and Pathology, The New York Hospital–Cornell Medical Center, New York, New York Search for other works by this author on: Oxford Academic Google Scholar American Journal of Clinical Pathology, Volume 70, Issue 4, 1 October 1978, Pages 637–641, https://doi.org/10.1093/ajcp/70.4.637 Published: 01 October 1978 Article history Received: 27 June 1977 Accepted: 29 July 1977 Published: 01 October 1978
