Paweł Marzęda

Medical University of Lublin

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Co-Authors

Jarogniew J. Łuszczki

Medical University of Lublin

Paula Wróblewska-Łuczka

Medical University of Lublin

Magdalena Florek-Łuszczki

Instytut Medycyny Wsi im. Witolda Chodźki

Katarzyna Załuska-Ogryzek

Medical University of Lublin

Małgorzata Drozd

Institut de Pharmacologie Moléculaire et Cellulaire

Agnieszka Góralczyk

Medical University of Lublin

Aleksandra Walczak

Medical University of Lublin

Michał Tchórz

Medical University of Lublin

Andrzej Kościuk

Medical University of Lublin

Hubert Bojar

Instytut Medycyny Wsi im. Witolda Chodźki

Cooperative Institutions

Medical University of Lublin

1 Military Clinical Hospital with Outpatient Clinic

Instytut Medycyny Wsi im. Witolda Chodźki

ORCID

John Paul II Catholic University of Lublin

Rzeszów University

Medical University of Lodz

Nicolaus Copernicus University

Jan Kochanowski University

Wojewódzki Szpital Zespolony

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Interactions among Lacosamide and Second-Generation Antiepileptic Drugs in the Tonic-Clonic Seizure Model in Mice

International Journal of Molecular Sciences (2021)

Katarzyna Załuska-Ogryzek Paweł Marzęda Paula Wróblewska-Łuczka Magdalena Florek-Łuszczki Zbigniew Plewa

Combination therapy with two or three antiseizure medications (ASMs) is sometimes a preferred method of treatment in epilepsy patients. (1) Background: To detect the most beneficial combination among three ASMs, a screen test evaluating in vivo interactions with respect to their anticonvulsant properties, was conducted on albino Swiss mice; (2) Methods: Classification of interactions among lacosamide (LCM) and selected second-generation ASMs (lamotrigine (LTG), pregabalin (PGB), oxcarbazepine (OXC), and topiramate (TPM)) was based on the isobolographic analysis in the mouse maximal electroshock-induced seizure (MES) model. Interactions among LCM and second-generation ASMs were visualized using a polygonogram; (3) Results: In the mouse MES model, synergy was observed for the combinations of LCM + TPM + PGB and LCM + OXC + PGB. Additivity was reported for the other combinations tested i.e., LCM + LTG + TPM, LCM + LTG + PGB, LCM + LTG + OXC, and LCM + OXC + TPM in this seizure model. No adverse effects associated with triple ASM combinations, containing LCM and second-generation ASMs were observed in mice; (4) Conclusions: The combination of LCM + TPM + PGB was the most beneficial combination among the tested in this study, offering synergistic suppression of tonic-clonic seizures in mice subjected to the MES model. Both the isobolographic analysis and polygonogram method can be recommended for experimental epileptology when classifying interactions among the ASMs.

Lacosamide

Oxcarbazepine

Pregabalin

Catalepsy

10.3390/ijms22115537

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Citations (4)

Synergy among oxcarbazepine , pregabalin and topiramate in the mouse maximal electroshockinduced seizure test – an isobolographic analysis

Journal of Pre-Clinical and Clinical Research (2018)

Katarzyna Załuska-Ogryzek Maria Kondrat-Wróbel Anna N. Panasiuk-Poterek Paweł Marzęda Aleksandra Walczak

1. Legge AW, Detyniecki K, Javed A, Hirsch LJ, Kato K, Buchsbaum R, et al. Comparative efficacy of unique antiepileptic drug regimens in focal epilepsy: An exploratory study. Epilepsy Res. 2018; 142: 73–80. CrossRef Google Scholar

Oxcarbazepine

Pregabalin

Antiepileptic drug

10.26444/jpccr/101578

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Citations (5)

Cannabidiol Interacts Antagonistically with Cisplatin and Additively with Mitoxantrone in Various Melanoma Cell Lines—An Isobolographic Analysis

International Journal of Molecular Sciences (2022)

Paweł Marzęda Paula Wróblewska-Łuczka Małgorzata Drozd Magdalena Florek-Łuszczki Katarzyna Załuska-Ogryzek

The medical application of cannabidiol (CBD) has been gathering increasing attention in recent years. This non-psychotropic cannabis-derived compound possesses antiepileptic, antipsychotic, anti-inflammatory and anxiolytic properties. Recent studies report that it also exerts antineoplastic effects in multiple types of cancers, including melanoma. In this in vitro study we tried to reveal the anticancer properties of CBD in malignant melanoma cell lines (SK-MEL 28, A375, FM55P and FM55M2) administered alone, as well as in combination with mitoxantrone (MTX) or cisplatin (CDDP). The effects of CBD on the viability of melanoma cells were measured by the MTT assay; cytotoxicity was determined in the LDH test and proliferation in the BrdU test. Moreover, the safety of CBD was tested in human keratinocytes (HaCaT) in LDH and MTT tests. Results indicate that CBD reduces the viability and proliferation of melanoma-malignant cells and exerts additive interactions with MTX. Unfortunately, CBD produced antagonistic interaction when combined with CDDP. CBD does not cause significant cytotoxicity in HaCaT cell line. In conclusion, CBD may be considered as a part of melanoma multi-drug therapy when combined with MTX. A special attention should be paid to the combination of CBD with CDDP due to the antagonistic interaction observed in the studied malignant melanoma cell lines.

HaCaT

MTT assay

Viability assay

Mitoxantrone

10.3390/ijms23126752

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Citations (24)

MMP targeting in the battle for vision: Recent developments and future prospects in the treatment of diabetic retinopathy

Life Sciences (2019)

Justyna Drankowska Michał Kos Andrzej Kościuk Paweł Marzęda Anna Boguszewska‐Czubara

10.1016/j.lfs.2019.05.038

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Citations (31)

Silent cerebral ischemic lesions in ablation-naïve patients with non-valvular atrial fibrillation: does the pulmonary vein anatomy matter?

Cardiology Journal (2024)

Andrzej Główniak Anna Drelich‐Zbroja Adam Tarkowski Paweł Marzęda Katarzyna Wojewoda

Background: Silent cerebral ischemic lesions (SCILs) detected by magnetic resonance imaging (MRI) can precede symptomatic stroke, the risk of which is increased five-fold in atrial fibrillation (AF) patients. In our study, we aimed to evaluate the initial incidence of SCILs in the population of patients referred for ablation due to symptomatic AF and to identify possible risk factors. Methods: A total of 110 patients, with a mean age (SD) of 59.9 (9.4) years, referred for ablation, were included in the study. In all patients, MRI was performed before the procedure to evaluate the incidence of SCILs in the ablation-naïve patients. Results: MRI revealed preexisting SCIL in 81/110 patients (73.6%). Notably, SCILs were found in all patients with CHA 2 DS 2 -VASc score ≥ 4. In univariable analysis, age (p < 0.001), CHA 2 DS 2 -VASc score (p = 0.001), hypertension (p = 0.01), and anticoagulation duration (p = 0.023) were identified as significant risk factors for SCILs, while the presence of anatomical variants of left-sided common pulmonary veins trunk (LCPV) had negative prognostic value (p = 0.026). Multivariable logistic regression analysis identified age (p < 0.001) as the risk factor of preexisting SCILs, whereas the presence of LCPV trunk was associated with significantly lower (p = 0.005) SCILs incidence. Conclusions: Silent cerebral ischemic lesions detected in MRI are frequent in the population of patients with non-valvular AF. The incidence of SCILs is higher in patients with long history of arrhythmia and higher CHA 2 DS 2 -VASc score. The relationship between the anatomy of pulmonary veins and the incidence of SCILs needs further investigation.

10.5603/cj.99142

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Citations (0)

Antiproliferative effects of LY-2183240 combined with various chemotherapeutic drugs in an isobolographic in vitro model of malignant melanoma

European Journal of Pharmacology (2024)

Paweł Marzęda Paula Wróblewska-Łuczka Magdalena Florek-Łuszczki Agnieszka Góralczyk Jarogniew J. Łuszczki

Mitoxantrone

MTT assay

Carboplatin

10.1016/j.ejphar.2024.176937

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Citations (1)

New psychoactive substances abuse and its clinical consequences - the case report

DOAJ (DOAJ: Directory of Open Access Journals) (2019)

Andrzej Kościuk Michał Kos Justyna Drankowska Marcelina Kaleta Paweł Marzęda

Psychoactive substance

Drugs of abuse

10.5281/zenodo.3372284

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Citations (1)

Additive suppression of tonic-clonic seizures in mice receiving the combination of carbamazepine, phenobarbital and valproate

Journal of Pre-Clinical and Clinical Research (2019)

Katarzyna Załuska-Ogryzek Paweł Marzęda Hubert Bojar Aleksandra Walczak Jarosław Chmielewski

1. Brodie MJ. Pharmacological treatment of drug-resistant epilepsy in adults: a practical guide. Curr Neurol Neurosci Rep. 2016;16:82. Google Scholar

Phenobarbital

Tonic (physiology)

Valproic Acid

10.26444/jpccr/109381

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Citations (4)

Role of vitamin A in health and illness

Journal of Pre-Clinical and Clinical Research (2019)

Paweł Marzęda Jarogniew J. Łuszczki

1. Shmarakov IO, Yuen JJ, Blaner WS. Carotenoid Metabolism and Enzymology. Carotenoids Hum. Heal., Totowa, NJ: Humana Press; 2013, p. 29–56. https://doi.org/10.1007/978-1-.... CrossRef Google Scholar

10.26444/jpccr/112376

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Citations (10)

Additive interactions between retigabine and oxcarbazepine in the chimney test and the model of generalized tonic-clonic seizures in mice

Journal of Epileptology (2016)

Zagaja Mirosław Barbara Miziak Katarzyna Załuska-Ogryzek Paweł Marzęda Drop Bartłomiej

Summary Introduction. Patients with pharmacoresistant epilepsy are usually treated with two or more antiepileptic drugs (AEDs). The search for therapeutically efficacious AED combinations is still a challenging issue for clinicians and epileptologists throughout the world. Aim. To determine the interaction profile for the combination of retigabine (RTG) and oxcarbazepine (OXC) in both, the model of tonic-clonic seizures, the maximal electroshock (MES)-induced seizure model and chimney test (motor performance) in adult male albino Swiss mice. Methods. Isobolographic analysis (type I) was applied to characterize interactions for the combination of RTG with OXC with respect to its anticonvulsant and acute side (neurotoxic) effects, as determined in the MES and chimney tests, respectively. Results. The combination of RTG with OXC at the fixed-ratios of 1:3, 1:1 and 3:1 produced additive interactions in the MES test in mice. Similarly, the combination of RTG with OXC at the fixed-ratio of 1:1 produced additive interaction with a tendency towards sub-additivity in the chimney test in mice. Measurement of total brain concentrations of both AEDs revealed that RTG did not affect total brain concentrations of OXC and inversely, OXC had no impact on RTG’s total brain concentrations, confirming pharmacodynamic interaction between the drugs. Conclusions. The additive pharmacodynamic interactions in both the MES and chimney tests in mice were observed for the combination of RTG with OXC.

Oxcarbazepine

Pharmacodynamics

10.1515/joepi-2016-0016

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Citations (2)