In hepatocellular carcinoma (HCC), CD8+ T-cell responses targeting tumor-associated antigens (TAA) are considered to be beneficial. However, only little is known about the molecular characteristics of TAA-specific CD8+ T cells in HCC due to their low frequency. In this study, we performed peptide/MHCI tetramer-based enrichment to characterize circulating TAA-specific CD8+ T cells ex vivo in HCC patients to gain a better understanding of their phenotypical and transcriptional characteristics.

Ex vivo

10.1055/s-0038-1677236

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Genetics in viral hepatitis: role of HLA class I and II alleles in hepatitis C virus infection

Springer eBooks (2007)

Robert Thimme Christoph Neumann‐Haefelin

10.1007/978-1-4020-6393-0_4

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Citations (1)

Deep spatial profiling of human COVID-19 brains

Zenodo (CERN European Organization for Nuclear Research) (2021)

Marius Schwabenland Henrike Salié Jovan Tanevski Saskia Killmer Marilyn Salvat Lago

Imaging mass cytometry data (txt files and folders containing single tiffs + cell masks) generated for the analysis of postmortem brains of COVID-19 patients and control groups (multiple sclerosis and patients who received ECMO therapy).

Profiling (computer programming)

2019-20 coronavirus outbreak

10.5281/zenodo.5018259

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P0479 : Naive-like CD8+ T cells specific for subdominant hepatitis B virus epitopes are present in chronically infected patients

Journal of Hepatology (2015)

A Schuch Muthamia M. Kiraithe Julia Lang‐Meli Christoph Neumann–Haefelin Robert Thimme

Subdominant

10.1016/s0168-8278(15)30687-5

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Deep Spatial Profiling of COVID-19 Brains Reveals Neuroinflammation by Compartmentalized Local Immune Cell Interactions and Targets for Intervention

SSRN Electronic Journal (2021)

Marius Schwabenland Henrike Salié Jovan Tanevski Saskia Killmer Marilyn Salvat Lago

COVID-19 can cause acute and chronic neurological symptoms. The underlying pathophysiological mechanisms, the involved immune cells, their spatial distribution, cellular interactions and the role of virus tropism remain largely unclear. Here, we deeply interrogated the brain stem and olfactory bulb in COVID-19 patients with imaging mass cytometry to understand the local immune response at a spatially resolved, high-dimensional single-cell level. We observed significant immune activation in the CNS and identified distinct phenotypes of T cells and microglial clusters, their presence in specific anatomical regions and context-specific cellular interactions. Microglial nodules and perivascular immune cell clusters constitute key sites of the local immune response, with viral antigen present in ACE2-expressing cells in the perivascular compartment. Disease-associated neuroinflammation is associated with astrogliosis and severe axonal damage as a structural basis for the neurologic deficits. Finally, we identify compartment- and cluster-specific immune checkpoints that can be targeted for future therapeutic interventions.Funding: This project was supported by grants from the Deutsche Forschungsgemeinschaft (DFG, GermanResearch Foundation) (SFB 992, SFB1160, SFB/TRR167, SFB/TRR179, German Excellency strategyCIBSS - EXC-2189– Project ID390939984) and special research funds from the Ministry for Science, Research and Art of Baden-Wuerttemberg dedicated to "COVID-19 research" and "Neuroinflammation".B.B. was further supported by DFG grant BE-5496/5-1 and M.P. was further supported by the Sobek Foundation, the Ernst-Jung Foundation, the Reinhart-Koselleck-Grant and Gottfried Wilhelm Leibniz-Prize.H.E.M. was supported by DFG ME-3644/5-1.Ethical Approval: The analyses were performed with the approval of the Institutional Review Boards (Ethic Committee of the Albert-Ludwigs-University, Freiburg: 322/20, 10008/09; Ethics Committee of the Hamburg Chamber of Physicians: WF-051/20, PV7311). The study was performed in agreement with the principles expressed in the Declaration of Helsinki (2013).Conflict of Interest: None to declare.

10.2139/ssrn.3765620

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Virushepatitis – wann daran denken?

DMW - Deutsche Medizinische Wochenschrift (2019)

Hendrik Luxenburger Robert Thimme Christoph Neumann‐Haefelin

Abstract Chronic viral hepatitis can remain unrecognized but may nevertheless lead to liver cirrhosis and hepatocellular carcinoma. Thus, patients with elevated liver enzymes as well as risk groups need to be screened and treated for viral hepatitis. These groups include, in particular, migrants from countries with high HBV or HCV prevalence, persons with previous or current intravenous drug use, and homosexual men. For HBV- or HCV-associated diseases, such as panarteriitis nodosa, cryoglobulinemic vasculitis or B-cell lymphoma, antiviral therapy may lead to remission. Prior to high-dose immunosuppressive therapy, especially with regimes containing rituximab, chronic or resolved HBV infection must be ruled out or antiviral prophylaxis may be required to avoid a potentially fatal HBV reactivation.

Antiviral Therapy

Viral Hepatitis

10.1055/a-0640-3627

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Protektiver Effekt von HLA-B27 auf den Verlauf der HIV- und Hepatitis-C-Virus-Infektion

DMW - Deutsche Medizinische Wochenschrift (2011)

Christoph Neumann–Haefelin

Drug Prescribing for Patients with Chronic Kidney Disease in General Practice: a Cross-Sectional Study

HLA-B27

Pathogenesis

Viral Hepatitis

10.1055/s-0031-1272531

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Exhaustion of CD39-Expressing CD8+ T Cells in Crohn’s Disease Is Linked to Clinical Outcome

Gastroenterology (2022)

Anna-Maria Globig Lena Sophie Mayer Maximilian Heeg Geoffroy Andrieux Manching Ku

10.1053/j.gastro.2022.06.045

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SARS-CoV-2-specific adaptive immune response after mRNA vaccination in liver transplanted patients

Zeitschrift für Gastroenterologie (2022)

Julia Lang‐Meli Hendrik Luxenburger Vivien Karl ElaheSalimi Alizei Benedikt Csernalabics

Background and aim Liver transplant recipients have an increased risk to develop severe or prolonged COVID-19. Therefore, these individuals particularly benefit from prophylactic vaccination. Recent studies demonstrated reduced antibody response rates upon COVID-19 mRNA vaccination in immunosuppressed individuals, however, little is known about the role of cellular immunity in this setting.

Antibody response

2019-20 coronavirus outbreak

10.1055/s-0041-1740815

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