Color me yellow: Poly(ADP-ribose) polymerases (PARPs) play a major role in cellular survival and maintenance of energy stores after genotoxic insult. The colorimetric PARP substrate ADP-ribose-pNP can be used to monitor PARP activity. By monitoring the production of p-nitrophenolate, the kinetic parameters of PARP-1, tankyrase, and PARP-4 could be evaluated. ADP=adenosine diphosphate, pNP=p-nitrophenoxy.
Gelb strahlt: Poly(ADP-Ribose)-Polymerasen (PARPs) spielen eine zentrale Rolle für das Überleben der Zellen und die Aufrechterhaltung von Energiespeichern nach einem gentoxischen Angriff. Das kolorimetrische PARP-Substrat ADP-Ribose-pNP kann zur Verfolgung der PARP-Aktivität genutzt werden. Anhand der Produktion von p-Nitrophenolat sind die Kinetikparameter von PARP-1, Tankyrase und PARP-4 ermittelbar. ADP = Adenosindiphosphat, pNP = p-Nitrophenoxy. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2001/2007/z603988_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Fluorescence studies of poly(2-methoxy-5-(4-(phenylazo)phenyl-4'-(1,10-dioxydecyl))-1,4-phenylenevinylene) (MPA-10-PPV), a PPV derivative with alkyl-tethered azobenzene side chains that was first synthesized by Yoshino and co-workers, are presented. The emission intensity of MPA-10-PPV in dilute solution is partially quenched by energy transfer from the PPV backbone to the azobenzene side chains, and the energy transfer is more efficient to the cis azobenzene isomer than to the trans. Because of this differential, the emission intensity can be reversibly modulated by controlling the isomeric form of the azobenzene side chain with light. The energy transfer process sensitizes the cis to trans azobenzene isomerization, and this additional isomerization pathway alters the composition of the azobenzene photostationary state in MPA-10-PPV.
