MÁ

Marı́a Ángeles Pérez

Universidad de Zaragoza

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i-10 index

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Document Type

Co-Authors

José Manuel García‐Aznar

Universidad de Zaragoza

M. Doblaré

Universidad de Zaragoza

Elena García‐Gareta

Instituto de Investigación Sanitaria Aragón

ÁA

Ángel Alberich‐Bayarri

Center For Biomarker Research In Medicine

Pilar Alamán‐Díez

Universidad de Zaragoza

Carlos Borau

Universidad de Zaragoza

Diego Sainz‐DeMena

Universidad de Zaragoza

Gabriele Nasello

KU Leuven

Roberto Sanz‐Requena

Hospital Regional de Antofagasta

Diego Alastruey-López

Instituto de Investigación Sanitaria Aragón

Cooperative Institutions

Universidad de Zaragoza

Instituto de Investigación Sanitaria Aragón

Instituto de Salud Carlos III

Consejo Superior de Investigaciones Científicas

Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine

Institute of Engineering

Instituto de Nanociencia y Materiales de Aragón

Universitat de València

Instituto Tecnológico de Aragón

Neuron BioPharma (Spain)

Author Statistics

Papers

Citation

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Parametric finite element model of medial patellofemoral ligament reconstruction model development and clinical validation

Journal of Experimental Orthopaedics (2019)

Vicente Sanchis‐Alfonso Diego Alastruey-López Gerad Ginovart Erik Montesinos-Berry Fabio García-Castro

Currently, there is uncertainty regarding the long-term outcome of medial patellofemoral ligament reconstructions (MPFLr). Our objectives were: (1) to develop a parametric model of the patellofemoral joint (PFJ) enabling us to simulate different surgical techniques for MPFLr; (2) to determine the negative effects on the PFJ associated with each technique, which could be related to long-term deterioration of the PFJ. A finite element model of the PFJ was created based on CT data from 24 knees with chronic lateral patellar instability. Patella contact pressure and maximum MPFL-graft stress at five angles of knee flexion (0, 30, 60, 90 and 120°) were analysed in three types of MPFLr: anatomic, non-anatomic with physiometric behaviour, and non-anatomic with non-physiometric behaviour. An increase in patella contact pressure was observed at 0 and 30° of knee flexion after both anatomic and non-anatomic MPFLr with physiometric behaviour. In both reconstructions, the ligament was tense between 0 and 30° of knee flexion, but at 60, 90 and 120°, it had no tension. In the third reconstruction, the behaviour was completely the opposite. A parametric model of the PFJ enables us to evaluate different types of MPFLr throughout the full range of motion of the knee, regarding the effect on the patellofemoral contact pressure, as well as the kinematic behaviour of the MPFL-graft and the maximum MPFL-graft stress.

Medial Patellofemoral Ligament

Patellofemoral joint

Biomechanics

10.1186/s40634-019-0200-x

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Citations (10)

In silico assessment of the bone regeneration potential of complex porous scaffolds

Marı́a Ángeles Pérez Reduan Asbai-Ghoudan Gabriele Nasello Stefaan W. Verbruggen Sergio Ruiz de Galarreta

Mechanical environment plays a crucial role in regulating bone regeneration in bone defects. Assessing the mechanobiological behavior of patient-specific orthopedic scaffolds in-silico could help guide optimal scaffold designs, as well as intra- and post-operative strategies to enhance bone regeneration and improve implant longevity. Additively manufactured porous scaffolds, and specifically triply periodic minimal surfaces (TPMS), have shown promising structural properties to act as bone substitutes, yet their ability to induce mechanobiologially-driven bone regeneration has not been elucidated. The aim of this study is to i) explore the bone regeneration potential of TPMS scaffolds made of different stiffness biocompatible materials, to ii) analyze the influence of pre-seeding the scaffolds and increasing the post-operative resting period, and to iii) assess the influence of patient-specific parameters, such as age and mechanosensitivity, on outcomes. To perform this study, an in silico model of a goat tibia is used. The bone ingrowth within the scaffold pores was simulated with a mechano-driven model of bone regeneration. Results showed that the scaffold’s architectural properties affect cellular diffusion and strain distribution, resulting in variations in the regenerated bone volume and distribution. The softer material improved the bone ingrowth. An initial resting period improved the bone ingrowth but not enough to reach the scaffold’s core. However, this was achieved with the implantation of a pre-seeded scaffold. Physiological parameters like age and health of the patient also influence the bone regeneration outcome, though to a lesser extent than the scaffold design. This analysis demonstrates the importance of the scaffold’s geometry and its material, and highlights the potential of using mechanobiological patient-specific models in the design process for bone substitutes.

10.31224/3617

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Citations (0)

Unusual tolerance to high temperatures in a new herbicide-resistant D1 mutant from Glycine max (L.) Merr. cell cultures deficient in fatty acid desaturation

Planta (2001)

Miguel Alfonso Inmaculada Yruela Susana Almárcegui Elena Torrado Marı́a Ángeles Pérez

Phosphatidylglycerol

Wild type

Cerulenin

10.1007/s004250000421

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Citations (93)

Decellularization of tumours: A new frontier in tissue engineering

Journal of Tissue Engineering (2022)

Elena García‐Gareta Marı́a Ángeles Pérez José Manuel García‐Aznar

Cancer is one of the leading causes of death worldwide. The tumour extracellular matrix (ECM) has unique features in terms of composition and mechanical properties, resulting in a structurally and chemically different ECM to that of native, healthy tissues. This paper reviews to date the efforts into decellularization of tumours, which in the authors' view represents a new frontier in the ever evolving field of tumour tissue engineering. An overview of the ECM and its importance in cancer is given, ending with examples of research using decellularized tumours, which has already indicated potential therapeutic targets, unravelled malignancy mechanisms or response to chemotherapy agents. The review highlights that more research is needed in this area, which can answer important questions related to tumour formation and progression to ultimately identify new and effective therapeutic targets. Within the near-future of personalized medicine, this research can create patient-specific tumour models and therapeutic regimes.

Decellularization

Regenerative Medicine

10.1177/20417314221091682

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Citations (21)

In vitro osteoinduction of human mesenchymal stem cells in biomimetic surface modified titanium alloy implants

Dental Materials Journal (2012)

Sonia Santander Clara Alcaine Jaber Lyahyai Marı́a Ángeles Pérez C. Rodellar

This article has been retracted by the Editorial Board of Dental Materials Journal due to violation of our publishing policies and procedures as of December 1, 2013.

Titanium alloy

Biocompatible material

Surface Modification

10.4012/dmj.2012-015

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Citations (14)

Hydrocolloids of Egg White and Gelatin as a Platform for Hydrogel-Based Tissue Engineering

Gels (2023)

Karinna Georgiana Pele H. Amaveda M. Mora Carlos Marcuello Anabel Lostao

Innovative materials are needed to produce scaffolds for various tissue engineering and regenerative medicine (TERM) applications, including tissue models. Materials derived from natural sources that offer low production costs, easy availability, and high bioactivity are highly preferred. Chicken egg white (EW) is an overlooked protein-based material. Whilst its combination with the biopolymer gelatin has been investigated in the food technology industry, mixed hydrocolloids of EW and gelatin have not been reported in TERM. This paper investigates these hydrocolloids as a suitable platform for hydrogel-based tissue engineering, including 2D coating films, miniaturized 3D hydrogels in microfluidic devices, and 3D hydrogel scaffolds. Rheological assessment of the hydrocolloid solutions suggested that temperature and EW concentration can be used to fine-tune the viscosity of the ensuing gels. Fabricated thin 2D hydrocolloid films presented globular nano-topography and in vitro cell work showed that the mixed hydrocolloids had increased cell growth compared with EW films. Results showed that hydrocolloids of EW and gelatin can be used for creating a 3D hydrogel environment for cell studies inside microfluidic devices. Finally, 3D hydrogel scaffolds were fabricated by sequential temperature-dependent gelation followed by chemical cross-linking of the polymeric network of the hydrogel for added mechanical strength and stability. These 3D hydrogel scaffolds displayed pores, lamellae, globular nano-topography, tunable mechanical properties, high affinity for water, and cell proliferation and penetration properties. In conclusion, the large range of properties and characteristics of these materials provide a strong potential for a large variety of TERM applications, including cancer models, organoid growth, compatibility with bioprinting, or implantable devices.

Gelatin

Biopolymer

10.3390/gels9060505

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Citations (17)

Primary Human Osteoblasts Cultured in a 3D Microenvironment Create a Unique Representative Model of Their Differentiation Into Osteocytes

Frontiers in Bioengineering and Biotechnology (2020)

Gabriele Nasello Pilar Alamán‐Díez Jessica Schiavi Marı́a Ángeles Pérez Laoise M. McNamara

Microengineered systems provide an in vitro strategy to explore the variability of individual patient response to tissue engineering products, since they prefer the use of primary cell sources representing the phenotype variability. Traditional in vitro systems already showed that primary human osteoblasts embedded in a 3D fibrous collagen matrix differentiate into osteocytes under specific conditions. Here, we hypothesized that translating this environment to the microfluidic scale creates a minimal functional unit to recapitulate osteoblast maturation towards osteocytes and matrix mineralization. Primary human osteoblasts were seeded in a type I collagen hydrogel, to establish the role of lower (2.5x10^5 cells/ml) and higher (1x10^6 cells/ml) cell density on their differentiation into osteocytes. A custom semi-automatic image analysis software was used to extract quantitative data on cellular morphology from brightfield images. The results are showing that cells cultured at a high density increase dendrite length over time, stop proliferating, exhibit dendritic morphology, upregulate alkaline phosphatase (ALP) activity and express the osteocyte marker dental matrix protein 1 (DMP1). On the contrary, cells cultured at lower density proliferate over time, do not upregulate ALP and express the osteoblast marker bone sialoprotein 2 (BSP2) at all timepoints. Our work reveals that microengineered systems create unique conditions to capture the major aspects of osteoblast differentiation into osteocyte with a limited number of cells. We propose that the microengineered approach is a functional strategy to create a patient-specific bone tissue model and investigate the individual osteogenic potential of the patient bone cells.

Bone sialoprotein

Osteocyte

DMP1

Matrix (chemical analysis)

10.3389/fbioe.2020.00336

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Citations (59)

Accelerating numerical simulations of strain-adaptive bone remodeling predictions

Computer Methods in Applied Mechanics and Engineering (2014)

Kasra Mohaghegh Marı́a Ángeles Pérez José Manuel García‐Aznar

Strain (injury)

Bone remodeling