Mohammed I. Mostafa

National Research Centre

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Heba A. S. Bazid

Menoufia University

Eman Masoud Abd El Gayed

Menoufia University

Salwa M. El Shebini

National Research Centre

Nihad H. Ahmed

National Research Centre

Sahar A. El-Masry

National Research Centre

Nermin Tayel

University of Sadat City

Mahmoud A.S. Afify

National Research Centre

Ayat N. Kamal

National Research Centre

M. A. Hammam

Menoufia University

Nayera E. Hassan

National Research Centre

Cooperative Institutions

National Research Centre

Menoufia University

National Water Research Center

Cairo University

Ain Shams University

Adam International Hospital

Research Institute of Ophthalmology

Agricultural Genetic Engineering Research Institute

Al-Azhar University

Alexandria University

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Assessment of macronutrients dietary intake, central adiposity among pre- and postmenopausal Egyptian women with benign and malignant breast tumors

Clinical Nutrition ESPEN (2023)

Osama Azmy Salwa M. El Shebini Nihad H. Ahmed Mohammed Abu‐Elghait Mohammed Mohammed Mohammed Gomaa

10.1016/j.clnesp.2023.03.011

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CD93 has a crucial role in pathogenesis of psoriasis

Journal of Cosmetic Dermatology (2021)

Wafaa Ahmed Shehata Alaa Hassan Maraee Nermin Tayel Asmaa S. Mohamed Eman Masoud Abd El Gayed

Psoriasis is a chronic, immune-related disorder; inflammation, higher rate of epidermal proliferation, and angiogenesis are the main pathognomonic features. Cluster of differentiation 93 (CD93), an angiogenic element, plays a role in cell adhesion regulation and has a putative function in inflammation.To assess CD93 immunohistochemical expression in psoriatic skin and the association of CD93 single nucleotide polymorphisms (SNPs) rs2749817 to disease pathogenesis and severity.This case-control study was done on 50 patients with psoriasis vulgaris beside 50 age- and sex-matched healthy controls. Assessment of psoriasis severity was done by Psoriasis Area and Severity Index (PASI) score. 3 mm punch skin biopsies were taken from every participant, and hematoxylin and eosin staining and immunohistochemical staining for CD93 antibody were done. Assessment of CD93 rs2749817 gene polymorphism by the TaqMan allelic discrimination assay technique (real-time PCR) was done.Immunohistochemical expression of CD93 showed membrano-cytoplasmic localization in both endothelial and inflammatory cells of cases and controls with significant more positivity in dermal endothelial and inflammatory cells of cases than controls (p = 0.001 and 0.014 respectively). Strong intensity was present in 18 of cases endothelial cells and 24 inflammatory cells with absence in controls (p = 0.001 for both) with significantly higher H-score and higher percent of positive cells (p = 0.001 for both). The TC genotype was lower in patients compared to control (p-value = 0.006) and CC genotype which was present only in cases (p-value = 0.021).Cluster of differentiation 93 has an essential role in psoriasis and an encouraging future therapy for psoriasis.

Pathogenesis

10.1111/jocd.14250

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Cryopreservation of rare ejaculate and testicular spermatozoa, a novel simple technique; the culture dish slice freezing: A pilot study.

Journal of Reproductive Medicine and Embryology (2024)

Medhat Amer Ihab Fikry Alshaimaa Aldsoqy Iman Attia Mohammed I. Mostafa

Objective: The ability to cryopreserve small numbers of spermatozoa retrieved by testicular sperm extraction (TESE) in males having nonobstructive azoospermia avoids the requirement for repeated surgery and promotes the preservation of fertility. The same applies to men with cryptozoospermia who may show few sperms only after concentrating their semen samples. Many techniques were proposed for freezing rare motile spermatozoa. In this pilot study we propose a new simple and safe technique that requires no specific commercial tools.Methodology: A new method is suggested for cryopreserving rare human spermatozoa using the culture dish slice. Results: Four cases were included in this pilot study. A total of 20 motile ejaculate sperms and 20 motile testicular sperms were frozen using the culture dish slice technique. Sperm retrieval, retrieval of motility and retrieval of usable sperms for ICSI were (100%, 25% and 70% respectively) for ejaculate sperms and (100%, 10% and 60% respectively) for testicular sperms. Conclusion: The culture dish slice technique would represent an easy, non-expensive method for rare sperm cryopreservation that necessitates no special commercial devices.

Testicular sperm extraction

Semen Analysis

Sperm Retrieval

10.21608/jrme.2024.267502.1011

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N-Acetyltransferase 2 gene polymorphism and its serum levels in vitiligo patients

Journal of Immunoassay and Immunochemistry (2024)

Heba A. S. Bazid M. A. Hammam Mona H. Keshk Mohammed I. Mostafa Eman Masoud Abd El Gayed

Although numerous mechanisms are involved in vitiligo pathogenesis, few studies correlate N-acetyltransferase 2 to this disease.

Vitiligo

Acetyltransferases

N-acetyltransferase

10.1080/15321819.2024.2415898

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Interleukin19 gene polymorphism and its serum level in acne vulgaris patients

Journal of Immunoassay and Immunochemistry (2021)

Heba A. S. Bazid Alaa Marae Nermin Tayel Rania Sleem Enas Mahrous

Numerous cytokines are involved in acne vulgaris pathogenesis, though few studies correlate interleukin IL-19 to acne vulgaris. So this study aimed to assess the IL-19 (rs 2243191) gene polymorphism and its serum level in acne vulgaris. This case-control study involved 90 acne vulgaris cases and 90 age- and sex-matched controls. Acne severity was assessed according to Global Acne Grading System (GAGS), and serum IL-19 was assessed by ELISA and IL-19 (rs 2243191) gene polymorphism was assessed by real time PCR. This study showed that acne cases had significantly higher IL-19 levels than controls. Also, its level was significantly higher in severe cases than moderate and mild cases. Regarding IL-19 gene polymorphism (rs 2243191), TT and CT genotypes were significantly higher in patients than in controls. The incidence of minor allele T was greater in patients than in controls. There were significant differences between IL-19 genotypes and disease severity. Serum IL-19 was significantly higher in genotypes TT and CT acne cases than in those with genotype CC. We concluded that TT genotype of IL-19 might be a hereditary risk factor for acne vulgaris development. It is associated with a high IL-19 serum level, which could be a marker of acne severity...

Gene polymorphism

Pathogenesis

10.1080/15321819.2021.1952425

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Assessment of cytochrome P450 1A1 gene polymorphism and vitamin A serum level in psoriasis vulgaris

Journal of Immunoassay and Immunochemistry (2023)

Heba A. S. Bazid Alaa Marae Nermin Tayel Etab Serag Hadeer Selim

Psoriasis is characterized by cutaneous hyperproliferation, secondary to immune system dysregulation. Vitamin A regulates the immune response and sustains epithelial tissue hemostasis. The CYP1A1 gene, has many biological actions, including vitamin A metabolism. To evaluate CYP1A1 gene polymorphism and serum vitamin A level in patients with psoriasis vulgaris, a case–control study involving two groups was conducted: group 1 (45 patients with psoriasis vulgaris) served as the cased group and group 2 (45 healthy participants who were sex and age matched) acted as the control group. CYP1A1 (rs1048943) gene polymorphism and vitamin A serum level were assessed by TaqMan allelic discrimination (PCR) and ELISA, respectively. AG genotype was present only in cases (22.2%), while AA genotype was present in all controls (P=.001). Vitamin A levels were lower in cases than in controls (32.0 ± 7.41 vs. 46.2 ± 15.7 μg/ml, respectively) (P<.001). AG genotype was associated with a lower vitamin A level (P=.001). The detected genotype difference between psoriasis patients and controls, which was associated with a lower serum vitamin A level and was also lower in more severe cases, suggests a role of the CYP1A1 gene and vitamin A in disease pathogenesis and prognosis.

Gene polymorphism

Pathogenesis

10.1080/15321819.2023.2189471

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Brief Overview of Dietary Intake, Gut Microbiota, Metabolic Markers and Research Opportunities in Sample of Egyptian Women

Research Square (Research Square) (2022)

Nayera E. Hassan Salwa M. El Shebini Sahar A. El-Masry Nihad H. Ahmed Ayat N. Kamal

Abstract Back ground: Metabolic syndrome (MetS) is a phenotype caused by interaction of host intrinsic factors such as genetics and gut microbiome, and extrinsic factors such as diet and lifestyle. Aim : To demonstrate the interplay of intestinal microbiota with obesity, MetS markers and some dietary ingredients among sample of Egyptian women. Methods: A cross-sectional study included 115 Egyptian women; 82 obese (59 without MetS and 23 with MetS) and 33 normal weight ones. Anthropometric assessment, 24 hours dietary recall, laboratory evaluation of liver enzymes (AST and ALT), leptin, short chain fatty acids (SCFA), C-reactive protein (CRP), fasting blood glucose (FBS), insulin and lipid profile, in addition to fecal microbiota analysis were done. Results : The obese women with MetS had the highest significant values of anthropometric and biochemical parameters, and consumed diet high in calories, protein, fat and carbohydrate, low in fiber and micronutrients. Bacteroidetes and Firmicutes was the abundant bacteria among different gut microbiota, with low Firmicutes/ Bacteroidetes Ratio, and insignificant differences between the 3groups. Firmicutes/ Bacteroidetes Ratio significantly correlated positively with total cholesterol and LDL-C and negatively with SCFA. Conclusion : The dietary factor, dysbiosis and the metabolic product short chain fatty acids have been implicated in causing metabolic defects.

Dysbiosis

10.21203/rs.3.rs-1427027/v1

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Brief overview of dietary intake, some types of gut microbiota, metabolic markers and research opportunities in sample of Egyptian women

Scientific Reports (2022)

Nayera E. Hassan Salwa M. El Shebini Sahar A. El-Masry Nihad H. Ahmed Ayat N. Kamal

Metabolic syndrome (MetS) is a phenotype caused by the interaction of host intrinsic factors such as genetics and gut microbiome, and extrinsic factors such as diet and lifestyle. To demonstrate the interplay of intestinal microbiota with obesity, MetS markers, and some dietary ingredients among samples of Egyptian women. This study was a cross-sectional one that included 115 Egyptian women; 82 were obese (59 without MetS and 23 with MetS) and 33 were normal weight. All participants were subjected to anthropometric assessment, 24 h dietary recall, laboratory evaluation of liver enzymes (AST and ALT), leptin, short chain fatty acids (SCFA), C-reactive protein, fasting blood glucose, insulin, and lipid profile, in addition to fecal microbiota analysis for Lactobacillus, Bifidobacteria, Firmicutes, and Bacteroid. Data showed that the obese women with MetS had the highest significant values of the anthropometric and the biochemical parameters. Obese MetS women consumed a diet high in calories, protein, fat, and carbohydrate, and low in fiber and micronutrients. The Bacteroidetes and Firmicutes were the abundant bacteria among the different gut microbiota, with low Firmicutes/Bacteroidetes ratio, and insignificant differences between the obese with and without MetS and normal weight women were reported. Firmicutes/Bacteroidetes ratio significantly correlated positively with total cholesterol and LDL-C and negatively with SCFA among obese women with MetS. Findings of this study revealed that dietary factors, dysbiosis, and the metabolic product short chain fatty acids have been implicated in causing metabolic defects.

Sample (material)

10.1038/s41598-022-21056-z

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Expression of cold-inducible RNA binding protein in psoriasis

Journal of Immunoassay and Immunochemistry (2022)

Heba A. S. Bazid Mohamed Shoeib Asmaa Nagy Elsayed Mohammed I. Mostafa May M. Shoeib

Psoriasis is an immune-mediated skin disease with a potential morbidity in patients. Cold-inducible RNA binding protein (CIRP) is a stress responsive protein having diverse roles in cancer and inflammation. This study aimed to evaluate the expression of CIRP, (serum and tissue), in psoriasis patients and to correlate this expression to the clinico-pathological data of the patients. The serum level and tissue expression of CIRP were compared between 20 patients and 20 healthy controls. Additionally, the association between CIRP level and various clinicopathological parameters was done. The serum level of CIRP was measured by enzyme-linked immunosorbent assay (ELISA) while its tissue expression was detected via immunohistochemistry. CIRP was expressed in the epidermis of all studied cases and controls with nuclear localization. A significant difference in its epidermal expression between lesional, perilesional cases and controls was observed. It was higher in control epidermis than perilesional skin and the lowest in lesional skin. Conversely, the serum CIRP level was significantly higher in psoriasis patients compared to healthy subjects. CIRP seemed to have a significant pathologic role in psoriasis patients with evident difference in its intracellular and extracellular expression levels suggesting a potential difference it its function.

Epidermis (zoology)

10.1080/15321819.2022.2039183

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Study of leptin gene polymorphism and leptin serum level in alopecia areata patients

Journal of Immunoassay and Immunochemistry (2022)

Heba A. S. Bazid M. A. Hammam Mohammed I. Mostafa Yasmine Gamal Eman Masoud Abd El Gayed

Leptin, produced by adipocytes, regulates metabolism, hunger, and immune response. The inflammatory role of leptin has been linked to autoimmune diseases. To assess leptin gene polymorphism and serum level in alopecia areata and their relation to metabolic syndrome (MS). This case-control study was conducted on 100 alopecia areata patients (50 with MS and 50 without MS) and 50 age- and gender-matched controls. Leptin gene polymorphism and serum level were assessed through the use of PCR and ELISA, respectively. GG genotype was the highest in AA with MS (54%), lower in AA without MS (42%), and the lowest in controls (20%). G allele was more expressed in cases, than in controls (P < .001). The serum leptin level was the highest in AA with MS, lower in AA without MS, and the lowest in controls (P value = 0.001). Leptin level was significantly higher in GG polymorphism than AG and AA. Leptin gene polymorphism (GG genotype) and serum level appear to play a significant role in AA. Absent difference regarding leptin gene polymorphism and MS might indicate a separate inflammatory role of leptin or the future risk of MS development in AA patients.

Gene polymorphism

10.1080/15321819.2022.2088294

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