Evelyn Lobo-Rivero

Centro Nacional de Sanidad Agropecuaria

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Belkis Corona

Centro Nacional de Sanidad Agropecuaria

Adrian Alberto Díaz-Sánchez

University of Saskatchewan

Lisset Roblejo‐Arias

Centro Nacional de Sanidad Agropecuaria

Ernesto Vega-Cañizares

Centro Nacional de Sanidad Agropecuaria

Osvaldo Fonseca‐Rodríguez

Umeå University

Ivette Espinosa-Castaño

Centro Nacional de Sanidad Agropecuaria

Pastor Alfonso

Centro Nacional de Sanidad Agropecuaria

Roxana Marrero‐Perera

Centro Nacional de Sanidad Agropecuaria

Ania Ramón-Martínez

Centro Nacional de Sanidad Agropecuaria

Anisleidy Pérez-Castillo

Centro Nacional de Sanidad Agropecuaria

Cooperative Institutions

Centro Nacional de Sanidad Agropecuaria

Bipar

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

École Nationale Vétérinaire d'Alfort

Centro de Ingeniería Genética y Biotecnología

ORCID

Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail

Systèmes d’élevage méditerranéens et tropicaux

Laboratoire de Recherches sur le Développement de l'Elevage

Universidad Técnica de Manabí

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COVID-19: the need for the strategic "ONE HEALTH" approach

Revista de salud animal (2020)

M.I. Percedo Abreu Pastor Alfonso Evelyn Lobo-Rivero Melissa Reyes I. Gutierrez Ravelo

Human health is increasingly interconnected with that of the animals in the environment they share, while the threat of emerging diseases arising at that interface increases as a result of the multiple and growing driving forces which propitiate that greater human-animalenvironmental interrelationship COVID-19 is an emerging zoonosis caused by SARS-CoV-2, a new coronavirus whose primary source is associated with bats Its rapid global expansion led the World Organization (WHO) to consider the disease as a Public Emergency of International Concern (PHEIC), for which the cross-sectoral approach is necessary The One Health strategy, promoted by the international organizations FAO, OIE and WHO, promotes collaborative efforts at all levels across multiple disciplines and sectors to achieve optimal health for people, animals and the environment, thus providing a conceptual framework for the development of cross-sectoral and multidisciplinary solutions to global health challenges This approach reviews the latest research reports on crucial aspects in the epidemiology of this new zoonosis, such as the characteristics of coronaviruses, their hosts, factors after crossing the interspecies barrier, and the undefined aspects of SARS-CoV-2 with potential implications for its prevention and control, as well as the lack of knowledge of the intermediate host of the virus, an important link in the epidemiological chain and the role that wild and domestic animal species, including pets, can have in the persistence of the virus in the environment and its potential re-emergence It also addresses the necessary cooperation between human and animal health services in activities to face COVID-19, while pointing out its negative economic impact on the entire food production and distribution chain This is an example of the importance of the holistic vision proposed by the One Health approach in the prevention and control of zoonoses, benefiting the achievement of the Sustainable Development Goals by all countries, by proposing that healthy people and animals live in a healthy planet There is a need of strengthening capacity building and cooperation between the human and animal health sectors, as well as their preparedness to face future health emergencies

Zoonosis

One Health

Pandemic

Global Health

Coronavirus

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Detección de Mollicutes en leche de tanques procedentes de la provincia Zamora-Chinchipe, Ecuador

Revista de salud animal (2018)

Natacha Ramírez-Sanmartín Luis Rodrigo-Saa María Irian Percedo Anisleidy Pérez-Castillo Evelyn Lobo-Rivero

There are several Mollicutes species that can produce mastitis. These infectious agents include Mycoplasma bovis , Mycoplasma canadense, Mycoplasma californicum , as well as Mycoplasma alkalences, Mycoplasma bovirhinis and Mycoplasma bovigenitalium . The objective of the present study was to detect the presence of Mollicutes in tank milk from herds in the Zamora-Chinchipe province, Ecuador. For the selection of the herds, a zoning was carried out in blocks of 25 km 2 , according to their ecological conditions (conglomerates). The number of herds for the study was determined by the EpiData program, for a 95 % confidence and a 5 % error. Sampling was carried out between May 2015 and December 2016, and the clusters of each canton to which the sampling was made were randomly chosen. In the selected herds, a milk sample (10 ml in sterile tube) was collected from each collection tank. Samples were analyzed by PCR with 16S rRNA universal primers of the Mollicutes class. In addition, the samples were analyzed by PCR with specific primers to detect the presence of M. bovis . Of the 143 herds researched, 25.87 % tested positive to Mollicutes (37/143 samples). However, the 37 samples positive to Mycoplasma spp. were negative to M. bovis . The results suggest the presence of other Mollicutes species in the herds.

Mycoplasma mycoides

Mycoplasmataceae

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Microfluidic PCR and network analysis reveals complex tick-borne pathogen interactions in the tropics

Parasites & Vectors (2024)

Cristian Díaz‐Corona Lisset Roblejo‐Arias Elianne Piloto‐Sardiñas Adrian Alberto Díaz-Sánchez Angélique Foucault‐Simonin

Abstract Background Ixodid ticks, particularly Rhipicephalus sanguineus s.l., are important vectors of various disease-causing agents in dogs and humans in Cuba. However, our understading of interactions among tick-borne pathogens (TBPs) in infected dogs or the vector R. sanguineus s.l. remains limited. This study integrates microfluidic-based high-throughput real-time PCR data, Yule's Q statistic, and network analysis to elucidate pathogen-pathogen interactions in dogs and ticks in tropical western Cuba. Methods A cross-sectional study involving 46 client-owned dogs was conducted. Blood samples were collected from these dogs, and ticks infesting the same dogs were morphologically and molecularly identified. Nucleic acids were extracted from both canine blood and tick samples. Microfluidic-based high-throughput real-time PCR was employed to detect 25 bacterial species, 10 parasite species, 6 bacterial genera, and 4 parasite taxa, as well as to confirm the identity of the collected ticks. Validation was performed through end-point PCR assays and DNA sequencing analysis. Yule's Q statistic and network analysis were used to analyse the associations between different TBP species based on binary presence-absence data. Results The study revealed a high prevalence of TBPs in both dogs and R. sanguineus s.l., the only tick species found on the dogs. Hepatozoon canis and Ehrlichia canis were among the most common pathogens detected. Co-infections were observed, notably between E. canis and H. canis . Significant correlations were found between the presence of Anaplasma platys and H. canis in both dogs and ticks. A complex co-occurrence network among haemoparasite species was identified, highlighting potential facilitative and inhibitory roles. Notably, H. canis was found as a highly interconnected node, exhibiting significant positive associations with various taxa, including A. platys , and E. canis , suggesting facilitative interactions among these pathogens. Phylogenetic analysis showed genetic diversity in the detected TBPs. Conclusions Overall, this research enhances our understanding of TBPs in Cuba, providing insights into their prevalence, associations, and genetic diversity, with implications for disease surveillance and management. Graphical abstract

Rhipicephalus sanguineus

Ehrlichia canis

Hepatozoon

Ehrlichiosis

Parasitology

Tick-borne disease

10.1186/s13071-023-06098-0

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Molecular detection and identification of spotted fever group rickettsiae in ticks collected from horses in Cuba

Medical and Veterinary Entomology (2020)

Adrian Alberto Díaz-Sánchez Neil B. Chilton Lisset Roblejo‐Arias Osvaldo Fonseca‐Rodríguez Roxana Marrero‐Perera

Abstract Spotted fever group (SFG) rickettsiae are obligatory intracellular bacteria that cause disease in humans and other animals. Ixodid ticks are the principal vectors of SFG rickettsiae. The present study aimed to determine the prevalence and species identity of SFG rickettsiae in ticks and horses from urban and rural areas of western Cuba using PCR assays. Tick samples, collected from 79 horses, consisted of 14 Amblyomma mixtum adults, 111 Dermacentor nitens adults and 19 pools of D. nitens nymphs (2–5 individuals/pool). The PCR results revealed the presence of Rickettsia spp. in 64% of the A. mixtum adults, 16% of the D. nitens adults, and 11% of the pooled samples of D. nitens nymphs. In contrast, Rickettsia spp. was not detected in any of the 200 horse blood samples included in this study. DNA sequence data of the rickettsial 17 kDa antigen gene showed that Rickettsia amblyommatis was present in A. mixtum ; and Rickettsia felis in D. nitens . This is the first report of R. felis in D. nitens in Cuba. The present study extends our knowledge of the potential vector spectrum and distribution of SFG rickettsiae pathogens in western Cuba.

10.1111/mve.12480

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Detection of microorganisms of the class Mollicutes associated to the respiratory-reproductive complex in horses from Mayabeque, Cuba.

Revista de salud animal (2019)

Ania Ramón-Martínez Fulgencio Pupo-Batista Lisset Roblejo‐Arias Antonio Díaz Sánchez Ileana Miranda-Cabrera

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Desarrollo de PCR especie-específicos para la detección de micoplasmas contaminantes de cultivos celulares

Revista de salud animal (2020)

Anisleidy Pérez Castillo Arianna Duque Ortiz Yaima Burgher Jose Antonio Agüero Fernández Mario Sergio Solis Castells

Mollicute contamination in cell cultures is common. Species such as Mycoplasma arginini , Mycoplasma salivarium , Mycoplasma fermentans , Mycoplasma hyorhinis , Mycoplasma orale , and Acholeplasma laidlawii are identified as causing 95 % of the contaminations. The present work is aimed at developing species-specific PCRs for the identification of the most frequent contaminating mycoplasma species in cell cultures. The critical parameters of PCR were optimized and the analytical sensitivity and specificity were determined. The optimum temperature for hybridization was set at 55oC; magnesium concentration at 1,5 mM. A range of 0,3-0,5 µM was selected for the primers. The analytical sensitivity of PCR was 1 pg/µL, while its analytical specificity was 100 %. Species-specific PCR was performed on 58 cell culture samples where the presence of Mollicutes was previously detected. As a result, Mycoplasma orale was detected as the most frequent species, followed by Mycoplasma fermentans , Acholeplasma laidlawii , Mycoplasma arginini , Mycoplasma hyorhinis , and Mycoplasma salivarium . The results of this study confirm that the species-specific PCR assays developed are feasible to use for the identification of mycoplasma species in cell cultures.

Mycoplasmataceae

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Microbiological and genotypic characterization of Pasteurella mutocida strains associated with the cunicular respiratory syndrome.

Revista de salud animal (2019)

Sonia Lugo-Marante Ivette Espinosa-Castaño Zenilda Zamora-Borges Layna Riera-Ojeda Ileana María Sosa-Testé

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Taller Nacional: Propuesta de una red piloto para la vigilancia de resistencia antimicrobiana en cepas patógenas y comensales de Escherichia coli de origen porcino

Revista de salud animal (2020)

Ivette Espinosa Castaño Yanet López Dorta Pedro Yoelvis de la Fé Emigdio Lemes Rosa Elena Hernández Fillor

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Seropositividad a Toxoplasma gondii en Felis catus del municipio Güines, Mayabeque, Cuba

Revista de salud animal (2021)

Daymara Mengana-Sánchez Ángel Entrena García Pastor Alfonso Evelyn Lobo-Rivero Belkis Corona

Toxoplasmosis is an important zoonosis due to its impact on human and animal health. Toxoplasma gondii is the causal agent of this disease. This protozoan has a complex biological cycle in which felines are the definitive hosts. The objective of this study was to evaluate the seropositivity to T. gondii in cats from Guines municipality, Mayabeque province, as well as to characterize the breeding practices of their owners and determine the main clinical symptoms in seropositive cats. . Thirty cats (19 females and 11 males) were studied. A total of 96.7 % (29/30) were seropositive to T. gondii , with no significant differences between sexes. The survey showed that 86.6 % (26/30) of the animals lived with other cats and went out on the streets daily. In addition, 52.6 % females and 100 % males were not sterilized. Paleness of visible mucous membranes was the most frequently observed sign (12/29, 41.37 %) in seropositive cats. It is concluded that there is a high frequency of positivity to Toxoplasma gondii in cats from Guines municipality, favored by certain breeding practices which increase the risk of exposure to T. gondii , benefit the dispersion of the protozoan and even represent the insufficient knowledge of their owners to adopt preventive measures against zoonosis. From the clinical signs observed, there is a need to improve tenure systems with implications for human and animal health and welfare, based on the One Health approach.

Zoonosis

Toxoplasmosis

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Termoestabilidad del ADN deshidratado de Mycoplasma gallisepticum

Revista de salud animal (2017)

Arianna Duque-Ortiz Damarys Relova-Vento Anisleidys Perez-Castillo Yaima Burger-Pulgarón Evelyn Lobo-Rivero

The preservation and storage of genetic material are of vital interest especially for mycoplasmas , since it is known that the traditional conservation methods reported for these species are not completely effective . The diagnosis of these microorganisms is carried out by standard and molecular methods ; among the latter is the Polymerase Chain Reaction test (PCR). One of the critical points of this test is the stability of the deoxyribonucleic acid (DNA). The aim of this work was to evaluate the thermal stability of fragments amplified by PCR, from dehydrated DNA, from the 16S rRNA gene for the detection of Mollicutes and from the 16S rRNA and mgc2 genes for the diagnosis of M. gallisepticum , targets commonly used in diagnosis. The product of each extraction was dried and stored at 4, 20 and 37 oC for a period of 90 days. The stability over time of the different regions of the diagnosis was assessed by amplification using PCR. The results showed that M. gallisepticum DNA was heat stable at room temperature for a period of 90 days. Furthermore, it was found that the purity of the extracted DNA did not affect its stability when kept dehydrated. The results showed that preserving the DNA of M. gallisepticum dehydrated allows storage and transfer without high costs and without affecting the quality of the samples for diagnosis.

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