Jovan Blagojević

University of Belgrade

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Milan Maletić

University of Belgrade

Zoran Stanimirović

University of Belgrade

Uroš Glavinić

University of Belgrade

Slobodanka Vakanjac

University of Belgrade

MĐ

Milan Đorđević

University of Nis

Branislav Kovačević

University of Novi Sad

Milorad Mirilović

University of Belgrade

Svetlana Nedić

University of Belgrade

Aleksandar Mašić

Zoetis (United States)

Jevrosima Stevanović

University of Belgrade

Cooperative Institutions

University of Belgrade

Croatian Veterinary Institute

University of Veterinary Medicine

University of Novi Sad

University of Belgrade – Faculty of Veterinary Medicine

Scientific Veterinary Institute "Novi Sad"

Institute of Physics Belgrade

Institut za filozofiju

University of Ljubljana

University of Antwerp

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Genome-Wide Analysis of Milk Production Traits and Selection Signatures in Serbian Holstein-Friesian Cattle

Animals (2024)

Marko Ristanić Minja Zorc Uroš Glavinić Jevrosima Stevanović Jovan Blagojević

To improve the genomic evaluation of milk-related traits in Holstein-Friesian (HF) cattle it is essential to identify the associated candidate genes. Novel SNP-based analyses, such as the genetic mapping of inherited diseases, GWAS, and genomic selection, have led to a new era of research. The aim of this study was to analyze the association of each individual SNP in Serbian HF cattle with milk production traits and inbreeding levels. The SNP 60 K chip Axiom Bovine BovMDv3 was deployed for the genotyping of 334 HF cows. The obtained genomic results, together with the collected phenotypic data, were used for a GWAS. Moreover, the identification of ROH segments was performed and served for inbreeding coefficient evaluation and ROH island detection. Using a GWAS, a polymorphism, rs110619097 (located in the intron of the CTNNA3 gene), was detected to be significantly (p < 0.01) associated with the milk protein concentration in the first lactation (adjusted to 305 days). The average genomic inbreeding value (FROH) was 0.079. ROH islands were discovered in proximity to genes associated with milk production traits and genomic regions under selection pressure for other economically important traits of dairy cattle. The findings of this pilot study provide useful information for a better understanding of the genetic architecture of milk production traits in Serbian HF dairy cows and can be used to improve lactation performances in Serbian HF cattle breeding programs.

Genome-wide Association Study

SNP genotyping

Runs of Homozygosity

Candidate gene

Bovine genome

SNP

Synteny

10.3390/ani14050669

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The Efficacy of Intravenous Application of the Mycobacterium Cell Wall Fraction (Amplimune) in the Treatment of Subclinical Mastitis Caused by S. Aureus

Ljubodrag Stanišić Zoran Stanimirović Branislav Kovačević Jovan Blagojević Svetlana Nedić

The aim of the study was to investigate the effect of the immunostimulant Mycobacterium Cell Wall Fraction (MCWF) on the treatment of S. aureus SCM by intravenous application. The study included 45 HF dairy cows 1-4 months after parturition divided into three groups (n=15 per group): the I group (MC+) - cows with S. aureus SCM treated with MCWF; the II group (MC-) - cows with S. aureus SCM, with no treatment; and III control group. Samples were collected 0th (I sample), 7th (II), and 14th day (III) from the day of SCM diagnosis and on day 21st (IV). A greater influx of leukocytes was confirmed into milk after 7 days after MCWF treatment in MC+ group, which was followed by increase of WBC and LYM in blood. These results support the hypothesis of effective action of MCWF, and in quarters with lower-grade infection, bacteriological cure was achieved. The MC- group had a statistically higher concentration of TBARS and CAT activity in milk, while MC+ group had lower blood serum LDH activity, which indicates a positive effect of the MCWF application and a lower exposure of the tissue to lipide peroxidation and inflammation caused by S. aureus. The application of MCWF would give new possibilities in the prevention and therapy of mammary gland diseases without fear of the presence of residues and the emergence of bacterial resistance. In future studies, the effects of local and systemic application of MCWF in the treatment of S. aureus SCM should be compared.

Subclinical infection

Fraction (chemistry)

10.2139/ssrn.4725620

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Use of Thymol in Nosema ceranae Control and Health Improvement of Infected Honey Bees

Insects (2022)

Uroš Glavinić Jovan Blagojević Marko Ristanić Jevrosima Stevanović Nada Lakić

Nosema ceranae is the most widespread microsporidian species which infects the honey bees of Apis mellifera by causing the weakening of their colonies and a decline in their productive and reproductive capacities. The only registered product for its control is the antibiotic fumagillin; however, in the European Union, there is no formulation registered for use in beekeeping. Thymol (3-hydroxy-p-cymene) is a natural essential-oil ingredient derived from Thymus vulgaris, which has been used in Varroa control for decades. The aim of this study was to investigate the effect of thymol supplementation on the expression of immune-related genes and the parameters of oxidative stress and bee survival, as well as spore loads in bees infected with the microsporidian parasite N. ceranae. The results reveal mostly positive effects of thymol on health (increasing levels of immune-related genes and values of oxidative stress parameters, and decreasing Nosema spore loads) when applied to Nosema-infected bees. Moreover, supplementation with thymol did not induce negative effects in Nosema-infected bees. However, our results indicate that in Nosema-free bees, thymol itself could cause certain disorders (affecting bee survival, decreasing oxidative capacity, and downregulation of some immune-related gene expressions), showing that one should be careful with preventive, uncontrolled, and excessive use of thymol. Thus, further research is needed to reveal the effect of this phytogenic supplement on the immunity of uninfected bees.

Nosema ceranae

Thymol

10.3390/insects13070574

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Citations (28)

Efficiency of Two Protocols of Resynchronization of Estrus and Ovulation in High-Producing Dairy Cows at Peak Lactation

Acta veterinaria (2022)

Milan Đorđević Jovan Blagojević Miodrag Radinović Marko Cincović Jelena Maletić

Abstract The reproductive efficiency of the cows was monitored after two resynchronization protocols: Ovsynch (OVS) and Double Ovsynch (DOS). The research initially included 70 HF cows who entered the first synchronization protocol – Presynch. Cows that did not conceive after the first synchronization were divided into two groups and introduced to two resynchronization protocols. In the first group of cows (n=35), the DOS protocol began with the application of GnRH on day 22 after the Presynch TAI (Timed Artificial Insemination), and seven days later pregnancy check-up was done and PGF 2α was applied only to non-pregnant cows (n=23), which remained in the study. In the second group of cows, the OVS protocol started on day 32 after Presynch TAI only in non-pregnant animals (n=20). Progesterone (P4) concentration was determined at the time of application of GnRH1, PGF 2α and GnRH2 in both groups of cows, and then 30 days after Resynch TAI, ultrasound pregnancy diagnosis was done. A higher percentage of pregnant cows were recorded in the OVS group compared to the DOS group (45% and 35%, respectively). The concentration of P4 in the serum of cows in the DOS group during the first measurement (GnRH1) was significantly higher than the value in cows that did not conceive (p<0.05), while in the third measurement (GnRH2) the average concentration of P4 in conceiving cows was significantly lower (p<0.001) compared to cows that did not coincive. The open days period was significantly longer in pregnant cows that were resynchronized using the DOS protocol compared to cows from the OVS protocol. In conclusion, the OVS protocol of estrus resynchronization in dairy cows proved to be more successful than the DOS protocol. However, considering the advantages the OVS, it is needed to determine which day of the sexual cycle is the best to start resynchronization.

Estrus synchronization

10.2478/acve-2022-0029

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Hematological, biochemical, and acid-base response of trotters to submaximal exercise at the end of the horse racing season

Veterinarski glasnik (2024)

Milica Stojković Jovan Blagojević D. Gvozdić Lazar Marković Dušan Bošnjaković

Depending on intensity, physical exercise in horses causes various changes in the parameters of hematological, biochemical, acid-base, and electrolyte status, which can affect the health and athletic performance of the horse. This study?s objective was to look at how submaximal exercise at the end of the racing season affected the horses? hematological, biochemical, acid-base, and electrolyte status markers. In this study, eight (n=8) trotters, aged 4?2 years, were involved. Venous blood samples were drawn from each horse by jugular puncture before and after exercise to determine hematologic, biochemical, acid-base and electrolyte parameters. The submaximal physical exercise in this study was two intervals of 2,000 m of slow trotting and two consecutive runs of 500 m at submaximal level. Hematocrit (HCT), red blood cell (RBC) and monocyte count, hemoglobin (HGB) concentration, aspartate aminotransferase (AST) activity, and glucose concentration increased significantly after the exercise. Additionally, significant decreases in venous blood pH, bicarbonate (HCO3-) and total CO2 (TCO2) concentration, base excess of the extracellular fluid (BEecf), and ionized Ca2+ (iCa2+) concentrations were established after exercise. In contrast, partial pressure of CO2 (pCO2), total concentration of weak acids (Atot), the anion gap (AG), and total protein and lactate concentrations were significantly higher after exercise. Considering the significant changes in the parameters of hematological, biochemical, and acid-base status after submaximal exercise, determining those parameters would be useful for monitoring the health and performance of trotters.

Acid–base homeostasis

Hemoconcentration

Bicarbonate

pCO2

Venous blood

Anion gap

Base excess

10.2298/vetgl230626002s

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Competition of disorder and electron-phonon coupling in 2H-TaSe$_{2-x}$S$_x$ ($0\le x\le 2$) as evidenced by Raman spectroscopy

arXiv (Cornell University) (2023)

Jovan Blagojević S. Djurdjić Mijin J Bekaert M. Opačić Yu Liu

The vibrational properties of 2H-TaSe$_{2-x}$S$_x$ ($0 \le x \le 2$) single crystals were probed using Raman spectroscopy and density functional theory calculations. The end members revealed two out of four symmetry-predicted Raman active modes, together with the pronounced two-phonon structure, attributable to the enhanced electron-phonon coupling. Additional peaks become observable due to crystallographic disorder for the doped samples. The evolution of the E$_{2g}^2$ mode Fano parameter reveals that the disorder has weak impact on electron-phonon coupling, which is also supported by the persistence of two-phonon structure in doped samples. As such, this research provides thorough insights into the lattice properties, the effects of crystallographic disorder on Raman spectra, and the interplay of this disorder with the electron-phonon coupling in 2H-TaSe$_{2-x}$S$_x$ compounds.

10.48550/arxiv.2312.09421

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Electrocatalytic Dioxygen Reduction by Carbon Electrodes Noncovalently Modified with Iron Porphyrin Complexes: Enhancements from a Single Proton Relay

Chemistry - A European Journal (2015)

Soumalya Sinha Michael S. Aaron Jovan Blagojević Jeffrey J. Warren

Oxygen reduction in acidic aqueous solution mediated by a series of asymmetric iron (III)-tetra(aryl)porphyrins adsorbed to basal- and edge- plane graphite electrodes is investigated. The asymmetric iron porphyrin systems bear phenyl groups at three meso positions and either a 2-pyridyl, a 2-benzoic acid, or a 2-hydroxyphenyl group at the remaining meso position. The presence of the three unmodified phenyl groups makes the compounds insoluble in water, enabling catalyst retention during electrochemical experiments. Resonance Raman data demonstrate that catalyst layers are maintained, but can undergo modification after prolonged catalysis in the presence of O2 . The introduction of a single proton relay group at the fourth meso position makes the asymmetric iron porphyrins markedly more robust catalysts; these molecules support higher sustained current densities than the parent iron tetraphenylporphyrin. Iron porphyrins bearing a 2-pyridyl group are the most active catalysts and operate at stable current densities ≥1 mA cm(-2) for over 5 h. Comparative analysis of the catalysts with different proton relays also is reported.

Carbon fibers

10.1002/chem.201502618

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Citations (50)

Motif of annunciation as biblical subtext in the poetry of Ivan V. Lalić

Kultura (2023)

Jovan Blagojević

Ivan V. Lalić, a poet who marked the last decades of the 20th century, belongs to the group of highly religious poets of the neo-symbolist movement. In the existing criticism, the biblical subtext of his poetry has been noticed, but some moments have not been given suf-fi-cient attention. And while the motif of Annunciation is much noticed and covered in criticism, some other theophanic aspects have been given little or no attention, even though they are precisely the context for proper understanding of Lalić's understanding of Annunciation. These are Old Testament scenes, primarily related to the forefather Abraham, Jacob, the exodus and the three young men in the fiery furnace. The author of the article devotes himself to these scenes, bringing them into connection with Lalić's understanding of Annunciation as a kind of point of resolution of cosmic, historical and personal tensions.

Subtext

Motif (music)

10.5937/kultura2379137b

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The efficacy of intravenous application of the Mycobacterium Cell Wall Fraction (AMPLIMUNE) in the treatment of subclinical mastitis caused by S. Aureus

Veterinary Research Communications (2024)

Ljubodrag Stanišić Zoran Stanimirović Branislav Kovačević Jovan Blagojević Svetlana Nedić

Subclinical infection

10.1007/s11259-024-10426-9

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Retained bovine placenta - various treatments and their effects

Veterinarski glasnik (2021)

Milan Maletić Filip Spasojevic Jovan Blagojević Nevenka Aleksić Slobodanka Vakanjac

Given the influence of retentio secundinarum (RS) on the reproductive performance of cows, the current work aimed to assess the effect of various approaches to the treatment of retained placenta on the main reproductive parameters, primarily on the interval from parturition to the first service, and the insemination index. The trial involved 120 dairy cows in which the placenta was not expelled within 24 h postpartum. The first group (G1, n=60) contained cows in which the retained placenta was not extirpated, but they were administered antibiotics. The second group (G2, n=60) comprised cows in which the placenta was removed manually, and were also administered antibiotics. At the end of the study, 53 cows remained in G1 and 54 cows in G2. According to medicament therapy, both groups were divided into three sub-groups treated with: procaine benzylpenicillin, chlortetracycline hydrochloride or ceftiofur. Comparison of the performance of G1 and G2 cows showed significantly better fertility parameters occurred in the animals from which the retained placenta was extracted manually. The pregnancy rate in the G2 group was remarkably higher than in the G1 group. The average service interval was significantly lower (p<0.05) in the G2 group compared to the G1 group. The best results were obtained with the i.m. administration of ceftiofur after the removal of the placenta.

Retained placenta

Ceftiofur

Benzylpenicillin

Metritis

10.2298/vetgl201205003m

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Citations (1)