O Estado do Rio de Janeiro esta integralmente inserido no Bioma Mata Atlântica. Estudos sobre a composicao floristica arborea da regiao Norte fluminense, publicados por Nascimento e colaboradores; resultaram na identificacao de um total de 210 especies vegetais distribuidas em 148 generos e 43 familias (Acta Botânica Brasileira, 2003, 17, 371-386). Dentre estas foi escolhida para o presente estudo a especie Annona dolabripetala, pertencente a familia Annonaceae. Estudos fitoquimicos e/ou farmacologicos com plantas desta familia eram centrados ate 1982, nos alcaloides isoquinolinicos. Depois de 1982 com a descoberta de “uvaricim”, a primeira acetogenina isolada de annonaceae e que apresentou atividade antileucemica in vivo ; foram intensificados trabalhos na busca desta nova classe de compostos que se mostraram altamente bioativos principalmente como antitumorais, inseticidas e pesticidas. A especie A. dolabripetala e popularmente conhecida como araticu, araticum, ata, biriba do mato e pinha do mato. E uma especie endemica do Brasil com distribuicao geografica no Nordeste, Sudeste e Sul. Na literatura nao se encontra estudo fitoquimico e/ ou farmacologico desta especie. O presente trabalho propoe, alem do estudo fitoquimico das folhas desta especie, em todos os extratos obtidos, ensaios de atividade antioxidante utilizando o DPPH (1,1 difenil-2-picril-hidrazil) como sequestrador de radicais e ensaios de atividade antineoplasica pelo metodo MTT. A coleta do material vegetal foi realizada na Reserva Biologica da Uniao localizada no municipio de Casemiro de Abreu em Maio/2013. Para preparacao dos extratos as folhas secas e moidas (203,268 g) foram submetidas a uma extracao sob pressao subcritica utilizando MeOH e H2O (9:1) como solventes, obtendo um extrato bruto de 74 g. Uma parte deste extrato 19,363 g foi submetido a particao com hexano, cloroformio e metanol obtendo-se fracoes com 1,0498 g; 0,3211 g; 1,9815 g respectivamente. Cada uma destas fracoes foi submetida a avaliacao do seu potencial de atividade biologica atraves de ensaio in vitro com celulas neoplasicas da linhagem U937 (linfoma), a investigacao do possivel mecanismo de acao por apoptose tambem foi realizado. Os resultados obtidos mostram que todos os extratos apresentam atividade em pelo menos uma das concentracoes testadas.
On March 11, 2020, the World Health Organization (WHO) officially declared the outbreak caused by the new coronavirus (SARS-CoV-2) a pandemic. The rapid spread of the disease surprised the scientific and medical community. Based on the latest reports, news, and scientific articles published, there is no doubt that the coronavirus has overloaded health systems globally. Practical actions against the recent emergence and rapid expansion of the SARS-CoV-2 require the development and use of tools for discovering new molecular anti-SARS-CoV-2 targets. Thus, this review presents bioinformatics and molecular modeling strategies that aim to assist in the discovery of potential anti-SARS-CoV-2 agents. Besides, we reviewed the relationship between SARS-CoV-2 and innate immunity, since understanding the structures involved in this infection can contribute to the development of new therapeutic targets. Bioinformatics is a technology that assists researchers in coping with diseases by investigating genetic sequencing and seeking structural models of potential molecular targets present in SARS-CoV2. The details provided in this review provide future points of consideration in the field of virology and medical sciences that will contribute to clarifying potential therapeutic targets for anti-SARS-CoV-2 and for understanding the molecular mechanisms responsible for the pathogenesis and virulence of SARS-CoV-2.
Over the centuries, nature has contributed to the basic needs of human beings.In particular, plants have cooperated as a source of drugs for the treatment of a broad spectrum of diseases because of the structural diversity of secondary metabolites.With strict attention to antineoplastic therapy, the search for anticancer drugs has increased in order to meet more specific and selective treatments.The acetogenins (ACGs), a class of secondary metabolites found exclusively in the family Annonaceae, are substances of great importance due to their well-known cytotoxic potency in human tumor cell lines in vitro, which can be exploited in designing anticancer agents.Among the 150 genera of Annonaceae family, genus Annona is in which is recorded the highest number of these substances.In order to identify the therapeutic potential and future directions for research, the purpose of this review is to provide data pertaining to the studies of cytotoxicity in vitro in cell lines of human cancer of isolated ACGs of Annona species in the period between 1989 and 2012 as well as basic information regarding the phytochemical studies of the same.
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