Sarvesh K. Soni

Punjab Engineering College

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Sampa Sarkar

RMIT University

Andrew S. Ball

RMIT University

Suresh K. Bhargava

RMIT University

Dinesh Kumar

Dr. S.R. Chandrasekhar Institute of Speech and Hearing

Selvakannan Periasamy

RMIT University

Vipul Bansal

RMIT University

Suresh K. Bhargava

University of Rajasthan

Kakarla Raghava Reddy

University of Sydney

Vivek B. Ravindran

RMIT University

Dhiman Sarkar

National Chemical Laboratory

Cooperative Institutions

RMIT University

MIT University

National Chemical Laboratory

RMIT Europe

Indian Institute of Chemical Technology

Academy of Scientific and Innovative Research

Banasthali University

Centre of Excellence for Advanced Materials

Yogi Vemana University

Savitribai Phule Pune University

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A QCM-based ‘on–off’ mechanistic study of gas adsorption by plasmid DNA and DNA–[Bmim][PF₆] construct

RSC Advances (2016)

Sarvesh K. Soni K. M. Mohibul Kabir Ravichandar Babarao Victoria E. Coyle Sampa Sarkar

The study of the adsorption behavior of disease markers such as ammonia (NH₃) and acetaldehyde (CH₃CHO) with biomaterials has been presented to enable the development of self-diagnosis technologies, among others.

10.1039/c6ra14759c

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Citations (3)

A modified approach to recover and enumerate Ascaris ova in wastewater and sludge

PLoS neglected tropical diseases (2019)

Vivek B. Ravindran Aravind Surapaneni Nicholas D. Crosbie Jonathan Schmidt Esmaeil Shahsavari

Ascaris

Enumeration

10.1371/journal.pntd.0007020

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Citations (9)

Cuboplex-Mediated Nonviral Delivery of Functional siRNA to Chinese Hamster Ovary (CHO) Cells

ACS Applied Materials & Interfaces (2021)

Sampa Sarkar Nhiem Tran Sarvesh K. Soni Zeyad Nasa Calum J. Drummond

Lipid nanoparticles of internal cubic symmetry, termed cuboplexes, are potential nonviral delivery vehicles for gene therapy due to their "topologically active" nature, which may enhance endosomal escape and improve delivery outcomes. In this study, we have used cationic cuboplexes, based on monoolein (MO) doped with a cationic lipid, for the encapsulation and delivery of antisense green fluorescent protein (GFP)—small interfering RNA (siRNA) into Chinese Hamster Ovary (CHO)—GFP cells. Agarose gel electrophoresis has confirmed the successful encapsulation of siRNA within cationic cubosomes, while synchrotron small-angle X-ray scattering (SAXS) demonstrated that the underlying cubic nanostructure of the particles was retained following encapsulation. The cationic cubosomes were shown to be reasonably nontoxic against the CHO-GFP cell line. Fluorescence-activated cell sorting (FACS) provided evidence of the successful transfection to CHO-GFP cells. Knockdown efficiency was strongly linked to the type of cationic lipid used, although all cubosomes had essentially the same internal nanostructure. The gene knockdown efficiency for some cationic cubosomes was shown to be higher than lipofectamine, which is a commercially available liposome-based formulation, while the controlled release of the siRNA from the cubosomes over a 72 h period was observed using confocal microscopy. This combination exemplifies the potential of cationic cuboplexes as a novel, nonviral, controlled-release delivery vector for siRNA.

10.1021/acsami.0c20956

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Detection of Helminth Ova in Wastewater Using Recombinase Polymerase Amplification Coupled to Lateral Flow Strips

Water (2020)

Vivek B. Ravindran Basma Khallaf Aravind Surapaneni Nicholas D. Crosbie Sarvesh K. Soni

Ascaris lumbricoides is a major soil-transmitted helminth that is highly infective to humans. The ova of A. lumbricoides are able to survive wastewater treatment, thus making it an indicator organism for effective water treatment and sanitation. Hence, Ascaris ova must be removed from wastewater matrices for the safe use of recycled water. Current microscopic techniques for identification and enumeration of Ascaris ova are laborious and cumbersome. Polymerase chain reaction (PCR)-based techniques are sensitive and specific, however, major constraints lie in having to transport samples to a centralised laboratory, the requirement for sophisticated instrumentation and skilled personnel. To address this issue, a rapid, highly specific, sensitive, and affordable method for the detection of helminth ova was developed utilising recombinase polymerase amplification (RPA) coupled with lateral flow (LF) strips. In this study, Ascaris suum ova were used to demonstrate the potential use of the RPA-LF assay. The method was faster (< 30 min) with optimal temperature at 37 °C and greater sensitivity than PCR-based approaches with detection as low as 2 femtograms of DNA. Furthermore, ova from two different helminth genera were able to be detected as a multiplex assay using a single lateral flow strip, which could significantly reduce the time and the cost of helminth identification. The RPA-LF system represents an accurate, rapid, and cost-effective technology that could replace the existing detection methods, which are technically challenged and not ideal for on-site detection in wastewater treatment plants.

Recombinase Polymerase Amplification

Ascaris suum

Ascaris

10.3390/w12030691

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Citations (13)

The Intracellular Co- localizations of Different Size of Gold Nanoparticles

TechConnect Briefs (2011)

Cemil Boyoglu Seyhan Boyoglu-Barnum Qin He Michelle Miller Shree R. Singh

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Microfluidic Synthesis of Nanoparticles and their Biosensing Applications

Critical Reviews in Analytical Chemistry (2016)

Jyoti Boken Sarvesh K. Soni Dinesh Kumar

We present an extensive overview of the evolution and progress made in the field of microstructures and nanostructures preparation using microfluidic techniques in recent times. A microfluidic system creates particles that are within a narrow range of shape and size distribution. It enables controlling the shape, size and composition of nanomaterials (NMs) for various applications. A brief evaluation of the advantages of both droplet-based and continuous flow synthesis of nanoparticles (NPs) is discussed in detail and compared with the traditional wet chemical batch synthesis approach. Due to increasing applications of biosensing, nanobiotechnology, nanomedicine and diagnostics devices, special attention should be paid to metal NPs developed through microfluidic routes.

Nanobiotechnology

Nanomaterials

10.1080/10408347.2016.1169912

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Citations (48)

A Review on the Current Knowledge and Prospects for the Development of Improved Detection Methods for Soil-Transmitted Helminth Ova for the Safe Reuse of Wastewater and Mitigation of Public Health Risks

Water (2019)

Vivek B. Ravindran Sarvesh K. Soni Andrew S. Ball

Climate change, increase in population and scarcity of freshwater have led to a global demand for wastewater reuse in irrigation. However, wastewater has to be treated in order to minimize the presence of pathogens, in particular, the ova of soil-transmitted helminthes (STHs). Limiting the transmission via removal of STH ova, accurate assessment of risks and minimizing the exposure to the public have been recommended by health regulators. The World Health Organization (WHO) guideline specifies a limit of ≤1 ova/L for safe wastewater reuse. Additionally, the Australian Guidelines for Water recycling (AGWR) recommend a hydraulic retention time of over 25 days in a lagoon or stabilization pond to ensure a 4 log reduction value of helminth ova and to mitigate soil-transmitted helminths associated risks to humans. However, the lack of fast and sensitive methods for assessing the concentration of STH ova in wastewater poses a considerable challenge for an accurate risk assessment. Consequently, it has been difficult to control soil-transmitted helminthiasis despite effective mass drug administration. This limitation can be overcome with the advent of novel techniques for the detection of helminth ova. Therefore, this review presents an assessment of the current methods to detect the viable ova of soil-transmitted helminths in wastewater. Furthermore, the review focuses on the perspectives for the emerging state-of-the-art research and developments that have the potential to replace currently available conventional and polymerase chain reaction based methods and achieve the guidelines of the WHO in order to allow the safe reuse of wastewater for non-potable applications, thereby minimizing public health risks.

Wastewater Reuse

10.3390/w11061212

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Citations (14)

Structural evaluation and catalytic performance of nano-Au supported on nanocrystalline Ce_0.9Fe_0.1O_2−δ solid solution for oxidation of carbon monoxide and benzylamine

RSC Advances (2014)

Putla Sudarsanam Selvakannan Periasamy Sarvesh K. Soni Suresh K. Bhargava Benjaram M. Reddy

Nano-Au supported on nanocrystalline Ce_0.9Fe_0.1O_2−δ solid solution was found to show excellent catalytic performance for both CO oxidation and benzylamine oxidation.

Nanocrystalline material

10.1039/c4ra07450e

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Citations (48)

Please do not adjust margins Please do not adjust margins Intrinsic therapeutic and biocatalytic roles of ionic liquid mediated self-assembled platinum-phytase nanosphere

Sarvesh K. Soni Sampa Sarkar Selvakannan Periasamy Selvakannan Periasamy Dhiman Sarkar

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Fluoride adsorption on a cubical ceria nanoadsorbent: function of surface properties

RSC Advances (2016)

Ankita Dhillon Tarun Kumar Sharma Sarvesh K. Soni Dinesh Kumar

Development of ceria with high fluoride loading capacity at pH 7.0 within 2 h at 1 g L⁻¹ adsorbent dose.

10.1039/c6ra16962g

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Citations (33)