Kenji Ohya

National Institute of Health Sciences

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Hideto Fukushi

Gifu University

Tsuyoshi Yamaguchi

Tottori University

Yukiko Hara‐Kudo

National Institute of Health Sciences

Tokuma Yanai

Gifu University

Tetsuo Asai

Gifu University

Kotaro Sawai

National Institute of Animal Health

Justice Opare Odoi

Council for Scientific and Industrial Research

Fumito Maruyama

Hiroshima University

Hirohito Ogawa

Okayama University

Toshihiro Ito

Nara Medical University

Cooperative Institutions

Gifu University

Hokkaido University

The University of Tokyo

National Institute of Infectious Diseases

National Institute of Health Sciences

Kyoto University

Tokyo University of Agriculture and Technology

Obihiro University of Agriculture and Veterinary Medicine

Osaka University

National Institute of Health Sciences

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Chlamydophila felisCF0218 Is a Novel TMH Family Protein with Potential as a Diagnostic Antigen for Diagnosis ofC. felisInfection

Clinical and Vaccine Immunology (2008)

Kenji Ohya Yu Takahara E Kuroda Saori Koyasu Shigeyuki Hagiwara

Chlamydophila felis is a causative agent of acute and chronic conjunctivitis and pneumonia in cats (feline chlamydiosis). Also, C. felis is a suspected zoonotic agent of such diseases as non-Chlamydia trachomatis conjunctivitis in humans, although this is controversial. At present, there is no serodiagnostic system that specifically detects C. felis infection conveniently. Current systems use antigens such as lipopolysaccharide that cross-react with all chlamydia species. In addition, it is difficult to distinguish between cats that are vaccinated with the commercial vaccine against C. felis and cats that are infected with C. felis. Here, we describe a new candidate diagnostic antigen for diagnosis of C. felis infection, CF0218, that was obtained by screening a genomic expression library of C. felis Fe/C-56 with C. felis-immunized serum. CF0218 was a putative transmembrane head (TMH) family protein with bilobed hydrophobic motifs at its N terminus, and orthologues of CF0218 were not found in the Chlamydophila pneumoniae or Chlamydia trachomatis genomes. The recombinant CF0218 was not recognized by antiserum against C. trachomatis, suggesting that CF0218 is C. felis specific. CF0218 transcription during the course of C. felis infection was confirmed by reverse transcription-PCR. By indirect immunofluorescence analysis, CF0218 was colocalized with the C. felis-formed inclusion bodies in the infected cells. The antibody response against CF0218 was elevated following C. felis infection but not by vaccination in experimentally vaccinated and infected cats. These results suggest that CF0218, a novel TMH family protein of C. felis, possesses potential as a C. felis infection-specific diagnostic antigen.

10.1128/cvi.00134-08

Cite

Citations (16)

Expression of human interferon alpha and tumor necrosis factor alpha in plants.

Takeshi Matsumura Kenji Ohya N. Itchouda Chihiro Sugimoto Jean‐Pierre Toutant

Alpha (finance)

Source

Cite

Citations (1)

Equine herpesvirus type 1 tegument protein VP22 is not essential for pathogenicity in a hamster model, but is required for efficient viral growth in cultured cells

Journal of Veterinary Medical Science (2015)

Ayaka Okada Satoko Izume Kenji Ohya Hideto Fukushi

VP22 is a major tegument protein of Equine herpesvirus type 1 (EHV-1) that is a conserved protein among alphaherpesviruses. However, the roles of VP22 differ among each virus, and the roles of EHV-1 VP22 are still unclear. Here, we constructed an EHV-1 VP22 deletion mutant and a revertant virus to clarify the role of VP22. We found that EHV-1 VP22 was required for efficient viral growth in cultured cells, but not for virulence in a hamster model.

Viral tegument

Wild type

10.1292/jvms.14-0648

Cite

Citations (7)

Chicken B Lymphoma DT40 Cells as a Useful Tool for in vitro Analysis of Pathogenic Infectious Bursal Disease Virus

Journal of Veterinary Medical Science (2008)

Kaori Terasaki Haruko Hirayama Christopher J. Kasanga Min Thein Maw Kenji Ohya

Susceptibility of DT40 cells to pathogenic field strains of infectious bursal disease virus (IBDV) including very virulent and classical virulent strains were studied. After the first and second passage of the virus in DT40 cells, IBDV-specific antigen was readily detected in DT40 cells inoculated with the pathogenic field strain infected bursal homogenates. Nucleotide sequence analysis in the VP2 hypervariable domain, which is critical for the virulence of IBDV, revealed no common amino acid substitutions among the pathogenic IBDVs in accordance with the propagation in DT40 cells. These results indicate that DT40 cells are a useful tool for rapid isolation of pathogenic field strains and successive in vitro analysis of IBDV.

Infectious bursal disease

10.1292/jvms.70.407

Cite

Citations (24)

Comparative Analysis of Fecal Microbiota in Grasscutter (Thryonomys swinderianus) and Other Herbivorous Livestock in Ghana

Microorganisms (2020)

Kiyonori Kawasaki Kenji Ohya Tsutomu Omatsu Yukie Katayama Yasuhiro Takashima

The grasscutter (also known as the greater cane rat; Thryonomys swinderianus) is a large rodent native to West Africa that is currently under domestication process for meat production. However, little is known about the physiology of this species. In the present study, aiming to provide information about gut microbiota of the grasscutter and better understand its physiology, we investigated the intestinal microbiota of grasscutters and compared it with that of other livestock (cattle, goat, rabbit, and sheep) using 16S rRNA metagenomics analysis. Similar to the other herbivorous animals, bacteria classified as Bacteroidales, Clostridiales, Ruminococcaceae, and Lachnospiraceae were abundant in the microbiome of grasscutters. However, Prevotella and Treponema bacteria, which have fiber fermentation ability, were especially abundant in grasscutters, where the relative abundance of these genera was higher than that in the other animals. The presence of these genera might confer grasscutters the ability to easily breakdown dietary fibers. Diets for grasscutters should be made from ingredients not consumed by humans to avoid competition for resources and the ability to digest fibers may allow the use of fiber-rich feed materials not used by humans. Our findings serve as reference and support future studies on changes in the gut microbiota of the grasscutter as domestication progresses in order to establish appropriate feeding methods and captivity conditions.

Lachnospiraceae

10.3390/microorganisms8020265

Cite

Citations (7)

GENETIC CHARACTERISTICS AND ANTIMICROBIAL RESISTANCE OF ESCHERICHIA COLI FROM JAPANESE MACAQUES (MACACA FUSCATA) IN RURAL JAPAN

Journal of Wildlife Diseases (2011)

Keiko Ogawa Keiji Yamaguchi Masatsugu Suzuki Toshio Tsubota Kenji Ohya

Escherichia coli was isolated from wild and captive Japanese macaques (Macaca fuscata) to investigate the risk of zoonotic infections and the prevalence of antimicrobial-resistant Escherichia coli in the wild macaque population in Shimokita Peninsula, a rural area of Japan. We collected 265 fresh fecal samples from wild macaques and 20 samples from captive macaques in 2005 and 2006 for E. coli isolation. The predominant isolates were characterized by serotyping, virulence gene profiling, plasmid profiling, pulsed-field gel electrophoresis (PFGE), and microbial sensitivity tests. In total, 248 E. coli strains were isolated from 159 fecal samples from wild macaques, and 42 E. coli were isolated from 17 samples from captive macaques. None of the virulence genes eae, stx, elt, and est were detected in any of the isolates. The relatedness between wild- and captive-derived isolates was low by serotyping, PFGE, and plasmid profiling. Serotypes O8:H6, O8:H34, O8:H42, O8:HUT, O103:H27, O103:HNM, and OUT:H27 were found in wild macaque feces; serotypes O157:H42 and O119:H21 were recovered from captive macaques. O-and H-serotypes of the 26 isolates were not typed by commercial typing antisera and were named OUT and HUT, respectively. Twenty-eight isolates had no flagellar antigen, and their H-serotypes were named HNM. Similarity of PFGE patterns between wild-derived isolates and captive-derived isolates was <70%. No plasmid profile was shared between wild-derived and captive-derived isolates. The prevalence of antimicrobial-resistant E. coli was 6.5% (n=62) in wild macaques, and these isolates were resistant to cephalothin. We conclude that wild Japanese macaques in Shimokita Peninsula were unlikely to act as a reservoir of pathogenic E. coli for humans and that antimicrobial-resistant E. coli in wild macaques may be derived from humans.

10.7589/0090-3558-47.2.261

Cite

Citations (6)

Genetic and antigenic analysis of <i>Chlamydia pecorum</i> strains isolated from calves with diarrhea

Journal of Veterinary Medical Science (2015)

Akifumi OHTANI Masahito Kubo Hiroshi Shimoda Kenji Ohya Tadashi IRIBE

Chlamydia pecorum (designated 22–58) was isolated in 2010 in HmLu-1 cells from the jejunum of a calf which died of necrotizing enterocolitis in Yamaguchi Prefecture, Japan. Immunohistochemical staining identified C. pecorum positive reactions in the jejunal villi. C. pecorum, designated 24–100, was isolated from the feces of a calf with diarrhea in another farm in Yamaguchi Prefecture in 2012. A significant increase in neutralizing antibody titers against C. pecorum was confirmed in paired sera. Nucleotide sequence identities of omp1 genes of the 2 isolates were 100%. The isolates were genetically and antigenically more closely related to C. pecorum Bo/Yokohama strain isolated from cattle with enteritis in Japan than to the other prototype strains, Bo/Maeda isolated from cattle with pneumonia and Ov/IPA isolated from sheep with polyarthritis. These results indicate that C. pecorum strains similar to 22–58 and 24–100 might be endemic in Yamaguchi Prefecture and cause enteric disease in cattle.

10.1292/jvms.14-0585

Cite

Citations (5)

Draft Genome Sequence of Mycobacterium virginiense Strain GF75, Isolated from the Mud of a Swine Farm in Japan

Genome Announcements (2018)

Toshihiro Ito Fumito Maruyama Kotaro Sawai Keiko Nozaki Keiko Otsu

Mycobacterium virginiense, a newly described species of the Mycobacterium terrae complex, is a cause of tenosynovitis and osteomyelitis in the United States. Here, we report the 4,849,424-bp draft genome sequence of M. virginiense strain GF75, isolated from a mud sample taken from a Japanese swine farm.

Strain (injury)

Sequence (biology)

Tenosynovitis

10.1128/genomea.00362-18

Cite

Citations (6)

Complete genome sequence of Chlamydia psittaci NRM_5 strain isolated from the fecal samples of a wild Indian ring-necked parakeet ( Psittacula krameri manillens is) in Japan

Microbiology Resource Announcements (2024)

Yukiko SASSA-O’BRIEN Chien-Fu Wu Yasuhiko Matsushita Kenji Ohya Hiromitsu Moriyama

We report here the whole-genome sequence of the

Parakeet

Strain (injury)

Chlamydia psittaci

10.1128/mra.01169-23

Cite

Citations (0)

Unique genomic sequences in a novelMycobacterium aviumsubsp.hominissuislineage enable fine scale transmission route tracing during pig movement

bioRxiv (Cold Spring Harbor Laboratory) (2022)

Tetsuya Komatsu Kenji Ohya Atsushi Ota Yukiko Nishiuchi Hirokazu Yano

Abstract Mycobacterium avium subsp. hominissuis (MAH) is one of the most prevalent mycobacteria causing non-tuberculous mycobacterial disease in humans and animals. Of note, MAH is a major cause of mycobacterial granulomatous mesenteric lymphadenitis outbreaks in pig populations. To determine the precise source of infection of MAH in a pig farm and to clarify the epidemiological relationship among pig, human and environmental MAH lineages, we collected 50 MAH isolates from pigs reared in Japan and determined draft genome sequences of 30 isolates. A variable number of tandem repeat analysis revealed that most pig MAH isolates in Japan were closely related to North American, European and Russian human isolates but not to those from East Asian human and their residential environments. Historical recombination analysis revealed that most pig isolates could be classified into SC2/4 and SC3, which contain MAH isolated from pig, European human and environmental isolates. Half of the isolates in SC2/4 had many recombination events with MAH lineages isolated from humans in East Asia. To our surprise, four isolates belonged to a new lineage (SC5) in the global MAH population. Members of SC5 had few footprints of inter-lineage recombination in the genome, and carried 80 unique genes, most of which were located on lineage specific-genomic islands. Using unique genetic features, we were able to trace the putative transmission route via their host pigs. Together, we clarify the possibility of species-specificity of MAH in addition to local adaptation. Our results highlight two transmission routes of MAH, one exposure on pig farms from the environment and the other via pig movement. Moreover, our study also warns that the evolution of MAH in pigs is influenced by MAH from patients and their residential environments, even if the MAH are genetically distinct. Highlights Variable number of tandem repeat analysis of Mycobacterium avium subsp. hominissuis (MAH) isolated from pigs ( n =50) were conducted. Draft genome sequences of MAH ( n =30) and genome analysis were conducted. Pig MAHs were genetically far from East Asian human isolates and close to those of Western countries. Novel MAH lineage which were transmitted farms by pig movement was found. Human MAH isolates influenced the evolution of pig isolates.

Lineage (genetic)

Domestic pig

10.1101/2022.04.04.487006

Cite

Citations (0)