Roberto Domı́nguez

Universidad Nacional Autónoma de México

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Roberto Chavira

Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

Marı́a Esther Cruz

Peruvian University of Applied Sciences

Leticia Morales‐Ledesma

Universidad Nacional Autónoma de México

Angélica Flores

Isabela State University

Carolina Morán

Benemérita Universidad Autónoma de Puebla

Elizabeth Vieyra

Universidad Nacional Autónoma de México

Mario Cárdenas

Ação Educativa

Leticia Morales

University of Kansas

Pablo Damián‐Matsumura

Universidad Autónoma Metropolitana

Deyra A. Ramírez

Autonomous University of Tlaxcala

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Universidad Nacional Autónoma de México

100

Benemérita Universidad Autónoma de Puebla

Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

University of Tennessee at Knoxville

Mexican Social Security Institute

Universidad de Montevideo

Autonomous University of Tlaxcala

Stanford University

University of Tennessee Medical Center

Universidad de Zaragoza

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Effects of a lesion in the dorsal raphe nuclei performed during the juvenile period of the female rat, on puberty

Brain Research Bulletin (1998)

Marı́a Elena Ayala J.J López Monroy Leticia Morales Marta E Castro Roberto Domı́nguez

Dorsal raphe nucleus

Estradiol valerate

10.1016/s0361-9230(98)00074-4

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Citations (19)

Effects of unilateral or bilateral superior ovarian nerve section in prepubertal rats on the ovulatory response to gonadotrophin administration

Journal of Endocrinology (1998)

Leticia Morales R Chávez ME Ayala Roberto Domı́nguez

The modulating effects of ovarian innervation reaching the ovary through the suspensory ovarian nerve on the reactivity of the ovaries to gonadotrophins were analysed. Juvenile rats (32 days old), with or without unilateral or bilateral section of the superior ovarian nerve, were injected with 8 iu of pregnant mare serum gonadotrophin (PMSG), 10 iu of human chorionic gonadotrophin (hCG) or with 8 iu of PMSG followed 56 h later with 10 iu of hCG. Treatments were given immediately after surgery or 4 days later, and the rats were killed on the day of first vaginal oestrus. In rats with unilateral section, treatment with PMSG did not induce full ovulatory response by the denervated ovary whether the treatment was applied immediately or 4 days after surgery (0/11 rats treated immediately ovulated vs 5/5 (sham) and 11/12 (control, P < 0.05 Fisher's exact probability test), and 4/19 did when treatment was done 4 days after surgery vs 8/10 (sham) and 11/12 (control, P < 0.05). The rats with bilateral section receiving the same hormonal treatment, PMSG administration, ovulated. The number of ova shed by the left ovary was similar to those of the control, while the right ovary released fewer ova. Stimulation with hCG immediately after unilateral section did not induce ovulation in normal or denervated ovary. When the treatment was applied 4 days after surgery, ovulation was observed only in the innervated ovary. In the rats with bilateral section, hCG injection induced ovulation in both ovaries. In those rats with unilateral section of the superior ovarian nerve, the treatment with PMSG + hCG given immediately after surgery resulted in a compensatory ovulation by the innervated ovary (the number of ova shed/ovulating animal was significantly higher than those released by control or sham-operated animals: left section, number of ova shed by the right ovary 7.6 +/- 0.3 vs 5.5 +/- 0.8 and 4.9 +/- 1 respectively, P < 0.05; right section, number of ova shed by the left ovary 10.2 +/- 0.6 vs 4.4 +/- 1.1 and 7.0 +/- 0.9, P < 0.05), while the denervated one showed a lower ovulation rate as well as a smaller number of ova shed than those by the control animals. When the hormonal replacement was given 4 days after surgery, such compensatory ovulation was observed in the left ovary of those rats with a section of the right nerve (14.3 +/- 2.6 vs 4.4 +/- 1.1 and 6.5 +/- 1.1, P < 0.05). When the PMSG + hCG treatment was applied to animals with bilateral section of the superior ovarian nerve, the ovulation rate by the right ovary was significantly lower than in control and sham-operated treated animals (2/10 vs 11/11 and 6/7, P < 0.05). Because the ovaries receive innervation through the superior ovarian nerve, the ovarian plexus and the vagus nerve, the results obtained in unilateral denervated animals suggest that the innervation of the ovary via the superior ovarian nerve regulates in a stimulatory way the effects of gonadotrophin resulting in ovulation. The ovulation induced by hormonal treatment of rats with bilateral section of the superior ovarian nerve suggests that the effects of bilateral section on ovulation are not the addition of the effects of left and right denervation, implying the existence of a modulatory effect in gonadotrophin action on ovulation via other neural pathways.

10.1677/joe.0.1580213

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Citations (42)

Experimental Polycystic Ovary Syndrome (PCOS) Induced by Estradiol Valerate (EV) Is Not Explained Only by the Increase in the Norepinephrine Ovarian Activity.

Biology of Reproduction (2008)

Roberto Domı́nguez Rosa Linares Gabriela Rosas Sara E. Cruz-Morales Leticia Morales

EV-induced PCOS is associated with an increase in peripheral sympathetic outflow, while sympathetic ovarian nerve denervation by bilateral section of the superior ovarian nerve (SON) results in ovulation. We have previously shown that the effects of unilateral section of the SON results in asymmetric response of ovulation and hormone secretion by the ovaries. The aims of present study were to analyze if the unilateral sympathetic denervation of one ovary by unilateral section of the SON, in a rat with PCOS induced by a single dose of EV injected on day 10 of life, overcomes the ovulatory ability in the denervated ovary and normalize progesterone (P), testosterone (T) and estradiol (E) serum levels. Oil and EV-treated animals on day 10 of life, on day 24 of life were submitted or not to the section of the right or left SON and autopsied at 90 days of age at the day of estrus. None of the EV-treated animals ovulated (0/11) while 9/12 of oil-treated ones did (p<0.05). All EV-treated animals developed PCOS. In oil-treated animals ovulation by the innervated ovary in rats with unilateral section of the SON was higher than in the denervated one (14/21 vs. 6/21, p<0.05). A similar result was observed in EV-treated animals with unilateral section of the SON (16/19 vs. 5/19, p<0.05). P levels in PCOS rats were similar to oil-treated animals (13.9±2.4 vs. 11.4±1.4), while T and E levels were significantly higher in PCOS rats (T 54.5±5.7 vs. 25.0±6.1; E 36.7±5.5 vs. 21.2±1.3, p<0.05). The unilateral section of the SON to oil-treated rats did not modify P and T serum levels. The section of the right SON resulted in lower E than in oil-treated and rats with section of the left SON (14.1±1.8 vs. 21.2±1.3, 23.1±1.3, p<0.05). The section of the right SON to EV-treated animals resulted in lower P levels than in EV-treated ones and in those with section of the left SON (5.2±0.6 vs. 13.9±2.4, 16.6±1.9, p<0.05). The unilateral section of the NOS to PCOS rats resulted in lower T and E levels than in control rats with PCOS (T 17.8±2.3, 20.8±4.4 vs. 54.5±5.7; E 15.9±1.2, 18.1±1.9 vs. 36.7±5.5, p<0.05). The section of SON resulted in a decrease in norepinephrine concentration in the denervated ovary and normal levels in the innervated one. The results support the idea of a neural communication between the ovaries and suggest that when such communication is modified, other neural information takes the control of ovulation in the innervated ovary and the hormone secretion modulation by the ovaries. Supported by UNAM-DGAPA IN 203307 and IN 200405.

Estradiol valerate

Testosterone propionate

10.1093/biolreprod/78.s1.178c

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Citations (0)

Oogenesis in Adult Mammals, Including Humans: A Review

Endocrine (2005)

Antonín Bukovský Michael R. Caudle Marta Svetlikova Jay Wimalasena María E. Ayala

Ovarian Cortex

Follicular atresia

Tunica albuginea (penis)

10.1385/endo:26:3:301

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Citations (94)

Dorsal and medial raphe nuclei participate differentially in reproductive functions of the male rat

Reproductive Biology and Endocrinology (2015)

María E. Ayala Diana E. Velázquez Juan L. Mendoza Juana Monroy Roberto Domı́nguez

Innervation of the hypothalamus and median eminence arise from the dorsal and medial raphe nuclei (DRN and MRN, respectively). The hypothalamus regulates the secretion of gonadotropins, which in turn regulate the reproductive function of males and females. However, it is not known the role of raphe nuclei in male reproductive function. Our goal was to investigate the role of the DRN and MRN in the regulation of the testicular function and secretion of gonadotropins in prepubertal rats. Dihydroxytryptamine (5,6-DHT) in ascorbic acid was used to chemically lesion the DRN or MRN. Rats were treated at 30 days-of-age and sacrificed at 45 or 65 days-of-age. Sham-treated controls were injected with ascorbic acid only. Negative controls were untreated rats. The damage induced by the 5,6-DHT was monitored in coronal serial sections of DRN and MRN; only the animals in which lesion of the DRN or MRN was detected were included in this study. As output parameters, we measured the concentrations of noradrenaline (NA), dopamine (DA) and serotonin (5-HT) in the anterior (AH) and medial (MH) hypothalamus by high performance liquid chromatography (HPLC); whereas, circulating concentrations of gonadotropins and sexual steroids were measured by radioimmunoassay. Seminiferous epithelium and sperm quality were also evaluated. Lesion of DRN or MRN does not induced changes in concentrations of LH, progesterone, and testosterone. Compared with the control group, the sham or lesion of the DRN or MRN did not modify noradrenaline or dopamine concentrations in the AH and MH at 45 or 65 days of age. Meanwhile, serotonin concentrations decreased significantly in lesioned rats. Lesion of DRN induced significantly lower concentrations of FSH regardless of age; similar lesion in the MRN had no impact on FSH levels. Sperm concentration and motility were significantly decreased in the same animals. The lesion of the MRN does not induced changes in the seminiferous epithelium or gonadotropin levels. Our results suggest that raphe nuclei regulate differentially the male reproductive functions. The DRN but not the MRN regulates the secretion of gonadotropins and testicular function.

Dorsal raphe nucleus

Median raphe nucleus

Median eminence

Raphe nuclei

5,7-Dihydroxytryptamine

10.1186/s12958-015-0130-0

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Citations (6)

Lateralization of the connections of the ovary to the celiac ganglia in juvenile rats

Reproductive Biology and Endocrinology (2009)

Carolina Morán F. Moreno Zarate J.L. Morán Anabella Handal Roberto Domı́nguez

Abstract During the development of the female rat, a maturing process of the factors that regulate the functioning of the ovaries takes place, resulting in different responses according to the age of the animal. Studies show that peripheral innervation is one relevant factor involved. In the present study we analyzed the anatomical relationship between the neurons in the celiac-superior mesenteric ganglia (CSMG), and the right or left ovary in 24 or 28 days old female pre-pubertal rats. The participation of the superior ovarian nerve (SON) in the communication between the CSMG and the ovaries was analyzed in animals with unilateral section of the SON, previous to injecting true blue (TB) into the ovarian bursa. The animals were killed seven days after treatment. TB stained neurons were quantified at the superior mesenteric-celiac ganglia. The number of labeled neurons in the CSMG of rats treated at 28 days of age was significantly higher than those treated on day 24. At age 24 days, injecting TB into the right ovary resulted in neuron stains on both sides of the celiac ganglia; whereas, injecting the left side the stains were exclusively ipsilateral. Such asymmetry was not observed when the rats were treated at age of 28 days. In younger rats, sectioning the left SON resulted in significantly lower number of stained neurons in the left ganglia while sectioning the right SON did not modify the number of stained neurons. When sectioning of the SON was performed to 28 days old rats, no staining was observed. Present results show that the number and connectivity of post-ganglionic neurons of the CSMG connected to the ovary of juvenile female rats change as the animal mature; that the SON plays a role in this communication process as puberty approaches; and that this maturing process is different for the right or the left ovary.

10.1186/1477-7827-7-50

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Citations (10)

The effects of sensorial denervation on the ovarian function, by the local administration of capsaicin, depend on the day of the oestrous cycle when the treatment was performed

Endocrine (2014)

Angélica Trujillo Leticia Morales Roberto Domı́nguez

Capsaicin

10.1007/s12020-014-0299-6

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Citations (5)

Studies on the Microheterogeneity of Anterior Pituitary Follicle-Stimulating Hormone in the Female Rat. Isoelectric Focusing Pattern Throughout the Estrous Cycle1

Biology of Reproduction (1988)

Alfredo Ulloa‐Aguirre R. Espinoza Pablo Damián‐Matsumura Fernando Larrea Alonso Vilches‐Flores

Anterior pituitary (AP) glands were removed from adult female rats at different times throughout the estrous cycle, and the isohormones of follicle-stimulating hormone (FSH) present within them were separated by isoelectric focusing in polyacrylamide gels (PAGE-IEF; pH range 3.0–8.0). Gel eluents were analyzed for FSH content by radioimmunoassay (RIA) and radioreceptor assay (RRA). All AP samples exhibited several peaks of FSH immunoactivity within a pH range of 6.2–4.0; the major peak constantly exhibited an isoelectric point (p1) of 4.9–4.5. To quantify differences in the IEF pattern of AP-FSH between the pituitaries collected during the different days of the cycle, each PAGE-IEF profile was divided into 7 regions (pI 7.0–6.3 = Area 1, 6.2–5.5 = Area 2, 5.4–5.0 = Area 3, 4.9–4.5 = Area 4, 4.4–4.0 = Area 5, 3.9–3.5 = Area 6, and <3.5 = Area 7), and the amount of FSH present within each was determined. In all APs collected at 0900 h of diestrus 1 (Dl) and 2 (D2), proestrus (P), and estrus (E); at 1300 h of D1, D2, and E; at 2200 h of P; and at 0200 h of E, the majority of FSH immunoactivity (37–55% of total FSH on gel) focused within Area 4, whereas Areas 2 and 3 contained minor amounts of FSH activity (11–26% and 14–24%, respectively). However, in glands collected at 1100 h, 1300 h, and 1800 h of the day of P, a significant increase in FSH content within Area 2 (30–32% of total on gel, p<0.05 vs. the remaining groups in the same area IP, Dl, D2, and E at 0900 h; Pat 2200 h; E at 0200 b; and Dl, D2, and E at 1300 hi) was clearly detected. The FSH peak found within this less acidic p1 are different from that found within Area 4 not only in its pI but also in receptor binding capacity. (RRA:RIA ratio Area 2 = 7.0 ± 2.7, Area 4 = 4.3 ± 1.0, p<0.05) and behavior in Concanavalin A (unbound to bound FSH ratio Area 2 = 0.14 ± 0.01, Area 4 = 0.40 ± 0.03, p<0.05). Enzymatic desialylation of a highly purified rat FSH preparation (NIADDK-FSH-I-6; p1 = 4.8) produced several peaks of immunoactive FSH, with more basic pls, that exhibited a higher affinity for Concanavalin A than that presented by the intact untreated molecule (unbound to bound FSH ratio of neuraminidase-treated FSH = 0.32 ± 0.01, control = 0.55 ± 0.04, p<0.05).

10.1095/biolreprod38.1.70

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Citations (45)

Effects of thymulin on spontaneous puberty and gonadotrophin-induced ovulation in prepubertal normal and hypothymic mice

Journal of Endocrinology (1999)

Lorena Hinojosa Roberto Chavira Roberto Domı́nguez P Rosas

The effects of thymulin administration beginning on days 19 or 24 of age on spontaneous puberty and gonadotrophin-induced ovulation were analysed in female normal and hypothymic mice. In normal and hypothymic mice, the daily administration of thymulin at 24 days of age resulted in a delay in the age of vaginal opening, with an increase in serum progesterone levels. Normal mice treated with 200 ng thymulin beginning on day 19 of age and injected with pregnant mare serum gonadotrophin (PMSG) 24 h later had an increase in ovulation rate, number of ova shed and weight of the ovaries. None of the hypothymic mice treated with thymulin on day 19 and PMSG on day 20 ovulated. PMSG treatment on day 25 induced ovulation in hypothymic mice. When these animals were injected previously with 200 ng thymulin, the number of ova shed by ovulating animals was lower than in PMSG-treated animals. Administration of thymulin and sequential injection of PMSG and human chorionic gonadotrophin 54 h later resulted in an increase in ovulatory response in comparison with those receiving only PMSG. The results suggest that thymulin plays a role in the regulation of spontaneous puberty through its effects on adrenal and ovarian endocrine functions. The increase in the ovarian PMSG response-treated animals, previously given thymulin, showed that this thymic hormone participates in the regulation of gonadotrophin secretion mechanisms and seems to be dose- and age-dependent. In hypothymic mice, neuroendocrine mechanisms regulating puberty are different from those of normal mice.

10.1677/joe.0.1630255

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Citations (22)

In Adult Rats With Polycystic Ovarian Syndrome, Unilateral or Bilateral Vagotomy Modifies the Noradrenergic Concentration in the Ovaries and the Celiac Superior Mesenteric Ganglia in Different Ways

Frontiers in Physiology (2019)

Rosa Linares Gabriela Rosas Elizabeth Vieyra Deyra A. Ramírez Daniel R. Velázquez

In rats with polycystic ovarian syndrome (PCOS) induced by estradiol valerate (EV) injection, sectioning of the vagus nerve in the juvenile stage restores ovulatory function, suggesting that the vagus nerve stimulates the onset and development of PCOS. We analyzed whether in adult rats, the role played by the vagus nerve in PCOS development is associated with the nerve's regulation of noradrenergic activity in the celiac superior mesenteric ganglion (CSMG). Ten-day-old rats were injected with corn oil [vehicle (Vh)] or EV (2 mg). At 76 days of age, rats injected with Vh or EV were subjected to sham surgery or the sectioning of one or both vagus nerves (vagotomy). The animals were sacrificed at 80-82 days of age at vaginal estrus smear. Compared to Vh-treated animals, EV-induced PCOS rats showed a lack of ovulation, the presence of follicular cysts, and a high concentration of testosterone, without changes in noradrenaline concentrations in the CSMG or ovaries. In PCOS rats, sham surgery lowered serum testosterone and noradrenaline concentrations in the CSMG but did not restore ovulation. In animals with PCOS, vagotomy lowered testosterone concentrations to a larger degree than in sham-surgery animals. The ovaries of rats with PCOS and vagotomy showed fresh corpora lutea, indicating ovulation. In EV-treated rats with unilateral vagotomy, the concentration of noradrenaline in the CSMG was similar to that in rats with PCOS and sham surgery, which did not ovulate, while in the ovaries of PCOS rats with left or bilateral vagotomy, the noradrenaline concentration was lower than that in sham-surgery-treated animals. Our results suggest that the vagus nerve regulates PCOS development through a different mechanism than the increase in the noradrenergic activity in the CSMG; however, in ovaries, the restoration of ovulation is associated with a decrease in ovarian noradrenaline.

Autonomic ganglion

10.3389/fphys.2019.01309

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Citations (16)