Roberto Domı́nguez

Universidad Nacional Autónoma de México

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Roberto Chavira

Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

Marı́a Esther Cruz

Peruvian University of Applied Sciences

Leticia Morales‐Ledesma

Universidad Nacional Autónoma de México

Angélica Flores

Isabela State University

Carolina Morán

Benemérita Universidad Autónoma de Puebla

Elizabeth Vieyra

Universidad Nacional Autónoma de México

Mario Cárdenas

Ação Educativa

Leticia Morales

University of Kansas

Pablo Damián‐Matsumura

Universidad Autónoma Metropolitana

Deyra A. Ramírez

Autonomous University of Tlaxcala

Cooperative Institutions

Universidad Nacional Autónoma de México

100

Benemérita Universidad Autónoma de Puebla

Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

University of Tennessee at Knoxville

Mexican Social Security Institute

Universidad de Montevideo

Autonomous University of Tlaxcala

Stanford University

University of Tennessee Medical Center

Universidad de Zaragoza

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Effects of Injecting Thymulin into the Anterior or Medial Hypothalamus or the Pituitary on Induced Ovulation in Prepubertal Mice

NeuroImmunoModulation (2005)

Lorena García Lorena Hinojosa Roberto Domı́nguez Roberto Chavira Patricia Rosas

In prepubertal mice, subcutaneous thymulin injection before equine chorionic gonadotrophin (eCG) treatment simulates ovulation; seemingly, the thymulin could be acting at the hypothalamus-pituitary axis level. Objective: This study was designed to analyze the effects of injecting thymulin into the hypothalamus or pituitary on induced ovulation of prepubertal mice. Method: Female mice, 19 days old, were anesthetized with ether and injected with saline solution or thymulin into the anterior or medial hypothalamus or the pituitary and treated with eCG when 20 days old. The ova shed were counted and serum concentrations of 17β-estradiol were measured. In the ovaries, the morphometrical analysis was performed and the atresia evaluated. Results: Ether anesthesia treatment blocked eCG-induced ovulation in almost all animals. Mice anesthetized and treated with eCG and gonadotrophin-releasing hormone (GnRH) or human chorionic gonadotrophin (hCG) ovulated a full quota of ova. Injecting saline solution into the anterior or medial hypothalamus or the pituitary did not reduce the blocking effects of ether anesthesia on induced ovulation, but the incidence of atretic follicles was higher. Injecting thymulin directly into the anterior hypothalamus did not restore ovulation, nor diminish the number of atretic follicles. In contrast, injecting thymulin into the medial hypothalamus restored the ovulation ratio and decreased the percentage of atretic follicles. Similar results were obtained by injecting thymulin into the pituitary, though thymulin treatment in the pituitary resulted in a higher number of ova shed and lower follicular atresia. Conclusion: The present results suggest that thymulin acts at the medial hypothalamus level, facilitating the release of GnRH and at the pituitary level regulating gonadotrophin release.

Induced ovulation

Follicular atresia

10.1159/000087111

Cite

Citations (15)

In rats with the polycystic ovary syndrome, the monoaminergic activity in the celiac superior mesenteric ganglion depends on the vagal innervation

Australasian Medical Journal (2017)

Rosa Linares Gabriela Rosas Elizabeth Vieyra Deyra A. Ramírez Yaneely A Guerrero

Monoaminergic

10.21767/amj.2017.2878

Cite

Citations (3)

Lack of sensorial innervation in the newborn female rats affects the activity of hypothalamic monoaminergic system and steroid hormone secretion during puberty

Endocrine (2013)

Ubaldo Quiróz Leticia Morales‐Ledesma Carolina Morán Angélica Trujillo Roberto Domı́nguez

Monoaminergic

Median eminence

10.1007/s12020-013-0055-3

Cite

Citations (4)

Intrinsic innervation of the ovary and its variations in the rat senescence process

Journal of Molecular Histology (2022)

Juan Manuel Bravo-Benítez Yolanda Cruz Rosa Angélica Lucio Berenice Venegas Alfonso Díaz

Ovarian functions decrease with perimenopause. The ovary has extrinsic innervation, but the neural influence on ovarian functions and dysfunction is not well-studied. The present study aimed to biochemically and morphometrically characterize the intrinsic neurons in ovaries from young adult, middle-aged, and senescent Long Evans CII-ZV rats (3, 12, and 15 months old, respectively). Ovaries were extracted from four rats of each age group (n = 12 total), cryopreserved, and processed for immunofluorescence studies with the primary NeuN/β-tubulin and NeuN/tyrosine hydroxylase (TH) antibodies. The soma area and number of intrinsic neurons in the ovarian stroma, surrounding follicles, corpus luteum, or cyst were evaluated. The intrinsic neurons were grouped in cluster-like shapes in ovarian structures. In senescent rats, the intrinsic neurons were mainly localized in the ovarian stroma and around the cysts. The number of neurons was lower in senescent rats than in young adult rats (p < 0.05), but the soma size was larger than in young adult rats. Immunoreactivity to TH indicated the presence of noradrenergic neurons in the ovary with the same characteristics as NeuN/β-tubulin, which indicates that they are part of the same neuronal group. Taken together, the findings indicate that the intrinsic neurons may be related to the loss of ovarian functions associated with aging.

NeuN

Senescence

Neurite

10.1007/s10735-022-10069-7

Cite

Citations (2)

Induction of Precocious Puberty in Newborn Female Guinea Pigs by a Positive Feedback Action of Estrogens

Hormone Research (1977)

Mabel Medero Roberto Domı́nguez

The existence of a positive feedback of estrogen on the gonadotrophin-releasing mechanism at birth, was tested in the female guinea pig. Estradiol benzoate (100 ng during the first 5 days of life) or HCG (100 RU during the first 5 days of life) induced a true precocious puberty, with similar characteristics to the control animals (60% of the animals ovulated at puberty age: corpora lutea present at the ovaries). The uterus was larger in ovulating than in nonovulating animals. Reserpine (2.5 mg/kg) on 15, 17 and 19 days of age did not modify the precocious puberty induced by estradiol at birth. It is concluded that exogenous or endogenous estrogen (induced by exogenous gonadotrophin administration) have a positive feedback action on the newborn female guinea pig to release gonadotrophin and develop puberty. These results are very different than those observed in the rat and differences can be related to the stage of somatic and nervous maturation attained by the animals at birth.

Reserpine

10.1159/000178804

Cite

Citations (1)

HYSTERECTOMY OF THE NEWBORN GUINEA-PIG, SUBSEQUENT EFFECTS ON THE OESTROUS CYCLE AND LIFE SPAN OF THE CORPORA LUTEA

Journal of Endocrinology (1975)

Washington Buño Emilia Carlevaro Laura Riboni Hortensia D'Albora Luisa de los Reyes

SUMMARY Longer oestrous cycles result from neonatal hysterectomy than from hysterectomy in adult life. Section and cauterization of the utero-vaginal union also prolonged the vaginal closure period up to an average of 55 days. The destruction of the mesometrium did not lengthen the oestrous cycle. Uterine autografts in hysterectomized newborn guinea-pigs did not prevent the long cycles.

Cauterization

Life span

10.1677/joe.0.0660233

Cite

Citations (5)

Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections

Journal of Visualized Experiments (2020)

Carlos‐Camilo Silva Montserrat Bolaños-Hurtado Cinthia Juárez-Tapia Angélica Flores Isabel Arrieta‐Cruz

Many experimental approaches have been used for studying the role of the brain in the regulation of ovulation. Examples include the lesion and deafferentation of neuronal groups, which are both invasive methods that permanently impair the integrity of the target area. These methods are accompanied by collateral effects that can affect the analysis of acute and temporal regulatory mechanisms. The stereotaxic implantation of guide cannulas aimed at specific brain regions, followed by a recovery period, allows researchers to microinject different drugs after the disappearance of the undesired effects of the surgery. Tetrodotoxin has been used to determine the roles of several brain areas in diverse physiological processes because it transiently inhibits the sodium-dependent action potentials, thus blocking all neural activity in the target region. This protocol combines this method with strategies for the assessment of the estrous cycle and ovulation to reveal the role of discrete brain regions in the regulation of ovulation at particular times of any given stage of the estrous cycle. Awake and unrestrained rats (Rattus norvegicus) were used to avoid the blocking effects that anesthetics and stress hormones exert on ovulation. This protocol can be easily adapted to other species, brain targets and pharmacological agents to study different physiological processes. Future improvements to this method include the design of a microinjection system using glass capillaries of small diameter instead of guide cannulas. This will reduce the amount of tissue damaged during the implantation and decrease the spread of the infused drugs outside the target area.

Tetrodotoxin

Microinjections

10.3791/61493

Cite

Citations (0)

Effect of steroid injection to newborn rats on serotonin activity in frontal cortex and raphe

Neuroreport (2003)

Roberto Domı́nguez Sara E. Cruz-Morales Milene Cristina de Carvalho Maristela Xavier Marcus Lira Brandão

The effects of injecting testosterone propionate or estradiol benzoate to newborn rats on dopaminergic and serotoninergic activity in the frontal cortex, dorsal and median raphe nucleus were analyzed when animals reached adulthood. High performance liquid chromatography was used to measure tissue levels of dopamine, serotonin and its metabolites. Activity was calculated as the metabolite/neurotransmitter ratio. An increase in androgen or estrogen levels at birth caused a significant decrease in serotoninergic activity in the frontal cortex and in the dorsal raphe nucleus, without causing apparent changes in dopaminergic activity; serotinergic activity in the median raphe nucleus was not affected. The results suggest that the transmission of DA and 5-HT in these structures are differentially influenced by early androgenization or estrogenization.

Dorsal raphe nucleus

Median raphe nucleus

Raphe nuclei

Raphe

10.1097/00001756-200303240-00014

Cite

Citations (21)

Effects of neonatal androgenization on the chromatofocusing pattern of anterior pituitary FSH in the female rat

Journal of Endocrinology (1990)

Alfredo Ulloa‐Aguirre Pablo Damián‐Matsumura R. Espinoza Roberto Domı́nguez Leticia Morales

ABSTRACT Anterior pituitary glands were removed from neonatally androgenized (100 μg testosterone propionate) female rats and normal controls at 5, 10, 18, 21, 30, 60 and 90 days of age, and the multiple forms of FSH present within them were separated by chromatofocusing (pH range 7·5–4·0). Additional pituitary glands from intact adult males (90 days old) were also studied for comparative purposes. All animal groups exhibited multiple forms of immunoactive FSH within a pH range of 7·5–4·0, as well as an additional FSH form obtained after the addition of 1·0 mol NaCl/l to the chromatofocusing column (salt peak). In animals 5–30 days old (controls and androgenized) the majority of FSH applied to the chromatofocusing columns was recovered within the salt peak (45-85% of total FSH immunoactivity recovered). However, as the animals aged, more FSH immunoactivity focused within less acidic regions (isoelectric point (pI) 5·9–5·0); pituitaries from animals 60 days old contained the greatest proportion of FSH focused within this pH range (controls, 39·2±0·6%; androgenized, 23·1 ±0·9% of total immunoactivity recovered; P < 0·03 vs animals 30 days old for both experimental groups). This shift towards less acidic FSH was attenuated in androgenized animals compared with the controls ( P <0·01). In control adult rats, the chromatofocusing distribution pattern of pituitary FSH varied according to the day of the oestrous cycle. Pituitary extracts from control rats decapitated during the morning of pro-oestrus, oestrus and day 1 of dioestrus exhibited the highest proportion of immunoactive FSH (23·2–28·8% of total) focused within a pH range of 5·9–5·0, whilst only 10·4–11·6% of FSH from androgenized rats and those on day 1 of dioestrus was recovered within this pH range ( P <0·05). In control animals decapitated during the morning of prooestrus and oestrus, 10–26% of FSH focused within the most alkaline region (pI 7·5–6·0); the chromatofocusing pattern of pituitary FSH from the neonatally androgenized animals was characteristic, in that no more than one peak (1·5±0·5% of total) was detected in this alkaline region. In the adult male rats, the majority of pituitary FSH eluted from the chromatofocusing columns within a pH of 4·9–4·0 (52·4±1·2% of total FSH immunoactivity) and the salt peak (pH <4·0) (33·1 ±2·4 of total). All FSH isoforms obtained after chromatofocusing represented α and β dimers as disclosed by size exclusion chromatography. The results strongly suggest that a cyclic or 'female' pattern of hypothalamic and gonadal secretion leads the anterior pituitary towards the production of less acidic FSH isoforms, whereas a tonic or 'androgenic' type of secretion, as that present in adult males and females with the androgen-induced anovulatory syndrome, leads more to the production of strongly acidic FSH isoforms. The finding of qualitative and quantitative differences among normally cycling and androgenized animals gives further support for the concept of the existence of a sexual dichotomy in terms of the type of FSH synthesized by the anterior pituitary gland. Journal of Endocrinology (1990) 126, 323–332

Chromatofocusing

10.1677/joe.0.1260323

Cite

Citations (18)

The Neural Signals of the Superior Ovarian Nerve Modulate in an Asymmetric Way the Ovarian Steroidogenic Response to the Vasoactive Intestinal Peptide

Frontiers in Physiology (2018)

Gabriela Rosas Rosa Linares Deyra A. Ramírez Elizabeth Vieyra Angélica Trujillo

The superior ovarian nerve (SON) provides neuropeptide-Y, norepinephrine and vasoactive intestinal peptide (VIP) to the ovaries. Ovarian steroidogenesis is modulated by the SON. In the cyclic rat, the acute steroidogenic response to ovarian microinjection of VIP is asymmetric and varies during the estrous cycle. In the present study, we analyze whether the differential effects of VIP in each ovary are modulated by the neural signals arriving through the SON. Cyclic female rats were submitted on diestrus-1, diestrus-2, proestrus, or estrus to a unilateral section of the SON, and immediately afterward, the denervated ovary was either microinjected or not with VIP. Animals were sacrificed 1 h after treatment. The injection of VIP into the left denervated ovary performed on diestrus-1 decreased progesterone levels in comparison with the left SON sectioning group; similar effects were observed on proestrus when VIP was injected into either of the denervated ovaries. Compared to the left SON sectioning group, VIP treatment into the left denervated ovary on diestrus-2 or proestrus decreased testosterone levels, whereas on diestrus-1, proestrus or estrus, the same treatment resulted in higher estradiol levels. Compared to the right SON sectioning group, VIP injected into the right denervated ovary yielded higher testosterone levels on diestrus-1 and estrus and lower testosterone levels on proestrus. VIP injection into the right denervated ovary increased estradiol levels on diestrus-2 or estrus while decreasing them on proestrus. Our results indicate that in the adult cyclic rat, the set neural signals arriving to the ovaries through the SON asymmetrically modulate the role of VIP on steroid hormone secretion, depending on the endocrine status of the animal. The results also support the hypothesis that the left and right ovary respond differently to the VIPergic stimulus.

Vasoactive

10.3389/fphys.2018.01142

Cite

Citations (15)