Maryam Ghobeh

Islamic Azad University, Science and Research Branch

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Parichehreh Yaghmaei

Islamic Azad University, Science and Research Branch

Masoud Homayouni Tabrizi

Islamic Azad University, Mashhad

Shahin Ahmadian

Shahid Beheshti University

Azadeh Ebrahim‐Habibi

Tehran University of Medical Sciences

Ali Es‐haghi

Islamic Azad University, Mashhad

Maryam Tabarzad

Shahid Beheshti University of Medical Sciences

Ali Akbar Meratan

Institute for Advanced Studies in Basic Sciences

Fariba Fathi

Islamic Azad University, Science and Research Branch

Mohammad‐Bagher Ebrahim‐Habibi

Petroleum University of Technology

Mohammad Reza Rezaei

University of Birjand

Cooperative Institutions

Islamic Azad University, Science and Research Branch

University of Tehran

Shahid Beheshti University of Medical Sciences

Shahid Beheshti University

Islamic Azad University, Tehran

Sharif University of Technology

Tarbiat Modares University

Shiraz University

Shiraz University of Medical Sciences

Tabriz University of Medical Sciences

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Inhibition of HEWL fibril formation by taxifolin: Mechanism of action

PLoS ONE (2017)

Mohsen Mahdavimehr Ali Akbar Meratan Maryam Ghobeh Atiyeh Ghasemi Ali Akbar Saboury

Among therapeutic approaches for amyloid-related diseases, attention has recently turned to the use of natural products as effective anti-aggregation compounds. Although a wealth of in vitro and in vivo evidence indicates some common inhibitory activity of these compounds, they don't generally suggest the same mechanism of action. Here, we show that taxifolin, a ubiquitous bioactive constituent of foods and herbs, inhibits formation of HEWL amyloid fibrils and their related toxicity by causing formation of very large globular, chain-like aggregates. A range of amyloid-specific techniques were employed to characterize this process. We found that taxifolin exerts its effect by binding to HEWL prefibrillar species, rather than by stabilizing the molecule in its native-like state. Furthermore, it's binding results in diverting the amyloid pathway toward formation of very large globular, chain-like aggregates with low β-sheet content and reduced solvent-exposed hydrophobic patches. ThT fluorescence measurements show that the binding capacity of taxifolin is significantly reduced, upon generation of large protofibrillar aggregates at the end of growth phase. We believe these results may help design promising inhibitors of protein aggregation for amyloid-related diseases.

Taxifolin

Amyloid (mycology)

Globular protein

Mechanism of Action

10.1371/journal.pone.0187841

Cite

Citations (46)

COMPARATIVE STUDY ON THE EFFECTS OF SEVERAL ERYTHROCYTE LYSING BUFFERS ON THE EXTRACTION OF GENOMIC DNA FROM FROZEN BLOOD SAMPLES IN EVALUATION OF TELOMERE LENGTH BY QPCR METHOD

Studies in Medical Sciences (2024)

Alireza Herischi Maryam Ghobeh Jafar Soleymani

genomic DNA

10.61186/umj.35.4.274

Cite

Citations (0)

Anti-Proliferative Activity of Magnetic Iron Oxide Nanoparticles Functionalized Glucose and Conjugated Cinnamon Against Gastric Cell Line

BioNanoScience (2024)

Mahsa Askarzadeh Esfahani Ali Salehzadeh Maryam Ghobeh

Zeta potential

MTT assay

IC50

Fragmentation

Iron oxide nanoparticles

10.1007/s12668-024-01562-2

Cite

Citations (1)

Preparation and Characterization of Allicin-Loaded Solid Lipid Nanoparticles Surface-Functionalized with Folic Acid-Bonded Chitosan: In Vitro Anticancer and Antioxidant Activities

Frontiers in Bioscience-Landmark (2023)

Faris J. Alyasiri Maryam Ghobeh Masoud Homayouni Tabrizi

Objective: This investigation aimed to increase the bioavailability and anticancer effects of allicin (AC) by encapsulating it in solid lipid nanoparticles (SLN) decorated with chitosan (CS)-conjugated folic acid (FA). Material and Methods: Nanoparticles (NPs) were synthesized by high-pressure homogenization, and then, Fourier-transform infrared (FTIR), Field-Emission Scanning Electron Microscopy (FESEM), Dynamic Light Scattering (DLS), and zeta potential methods were used to determine their physicochemical characteristics. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to assess the effect of toxicity and flow cytometry, while fluorescent staining methods were used to investigate the type of cell death. Real-time quantitative polymerase chain reaction (qPCR) was used to evaluate the expression levels of apoptotic genes: Bcl-2, and caspase-8. Results: The presence of AC-SLN-CS-FA with a spherical morphology, an average size of 86.7 ± 9.4 nm, uniform distribution (0.31), a surface charge of +21.3 ± 13.3 mV, an encapsulation percentage of 86.3%, and a folate binding rate of 63% confirmed the success of the preparation method. Suppression of MCF-7 cancer cells and non-toxicity of AC-SLN-CS-FA on Human foreskin fibroblast (HFF) normal cells were confirmed by cytotoxic assay. The results of flow cytometry revealed that the cells were arrested in the sub-G1 phase, and the activation of the intrinsic apoptosis pathway was confirmed by the results of real-time qPCR. Conclusions: In general, AC-SLN-CS-FA has the potential to prevent free radicals and trigger apoptosis in cancer cells by activating the intrinsic apoptosis pathway; thus, making it a promising subject in preclinical research.

Zeta potential

MTT assay

10.31083/j.fbl2807135

Cite

Citations (7)

Association of Blood Lead Level with Thyroid Hormones and Blood Factors in Iranian Traffic Officers

Biomacromolecular Journal (2018)

Hanieh Babaei Maryam Ghobeh Maryam S. Daneshpour

Lead has many applications in the industry, but does not play a vital physiological role in the human body. On the other hand, lead has shown undesirable effects on the nervous, gastrointestinal, and endocrine systems, and people who are highly exposed to this element, due to their occupations or residencies, are affected by its harmful effects. Therefore, we designed a cross-sectional study on traffic officers with high exposure level to lead in Tehran, Iran, to specify the effect of lead on their levels of thyroid hormones and blood cells. The blood lead concentration was measured using Lead Analyzer and Flame Atomic Absorption spectrometry. The levels of levels of thyroid hormones and blood cells were measured using KX-21N and ADVIA Centaur XPT (Immunoassay system) instruments, respectively. The obtained results demonstrated that there was no significant association between blood lead levels and levels of thyroid hormones, number of blood cells, and related blood cells parameters. However, there was a weak association between blood lead levels and the age of participants (r = 0.25). Further studies with higher sample size could confirm these results.

Blood lead level

Lead (geology)

Source

Cite

Citations (0)

Anti-Microbial Peptides: Strategies of Design and Development and Their Promising Wound-Healing Activities

Molecular Biology Reports (2022)

Fariba Fathi Maryam Ghobeh Maryam Tabarzad

10.1007/s11033-022-07405-1

Cite

Citations (13)

The Anticancer Impact of Ananas Leaves Extract-synthesized Folate-linked Chitosan Coated CeO2 Nanoparticles on Human Breast Cancer Cells

Journal of Polymers and the Environment (2023)

Jasim Kamil Kadhim Alkhafagi Masoud Homayouni Tabrizi Maryam Ghobeh

ABTS

DAPI

MTT assay

MCF-7

Ananas

10.1007/s10924-023-02904-z

Cite

Citations (4)

The Anticancer, Anti-metastatic, Anti-oxidant, and Anti-angiogenic Activity of Chitosan-coated Parthenolide/Bovine Serum Albumin Nanoparticles

Journal of Inorganic and Organometallic Polymers and Materials (2023)

Ahmed Ibrahim Albosultan Maryam Ghobeh Masoud Homayouni Tabrizi

Parthenolide

Bovine serum albumin

ABTS

Chorioallantoic membrane

Zeta potential

10.1007/s10904-023-02541-y

Cite

Citations (3)

Design and Evaluation of a Novel Anti-microbial Peptide from Cathelicidin-2: Selectively Active Against Acinetobacter baumannii

Deleted Journal (2023)

Fariba Fathi Maryam Ghobeh Farshad H. Shirazi Maryam Tabarzad

Background: Infections caused by pathogenic microorganisms have increased the need for hospital care and have thus represented a public health problem and a significant financial burden. Classical treatments consisting of traditional antibiotics face several challenges today. Anti-microbial peptides (AMPs) are a conserved characteristic of the innate immune response among different animal species to defend against pathogenic microorganisms. Objectives: In this study, a new peptide sequence (mCHTL131-140) was designed using the in silico approach. Methods: Cathelicidin-2 (UniprotID: Q2IAL7) was used as a potential antimicrobial protein, and a novel 10 - 12 amino acids sequence AMP was designed using bioinformatics tools and the AMP databases. Then, the anti-bacterial, anti-biofilm, and anti-fungal properties of the peptide, as well as its hemolytic activity and cytotoxicity towards human fibroblast (HDF) cells, were investigated in vitro. Results: Online bioinformatics tools indicated that the peptide sequence could have anti-bacterial, anti-viral, anti-fungal, and anti-biofilm properties with little hemolytic properties. The experimental tests confirmed that mCHTL131-140 exhibited the best anti-bacterial properties against Acinetobacter baumannii and had fair anti-fungal properties. Besides, it did not cause red blood cell lysis and showed no cytotoxicity towards HDF cells. Conclusions: In general, the designed peptide can be considered a promising AMP to control hospital-acquired infections by A. baumannii.

Acinetobacter baumannii

Cathelicidin

10.5812/ijpr-141920

Cite

Citations (3)

Investigation of the expression of Cis P‐tau and Pin1 proteins following air pollution induction in the brain tissue of C57BL/6 mice

Biotechnology and Applied Biochemistry (2024)

Sheyda Shahpasand Seyyed Hossein Khatami Sajad Ehtiati Farzaneh Salmani Tayebe Zarei

Abstract Alzheimer's disease (AD) is a multifactorial disease in which environmental factors play a role. Among environmental factors, air pollution is a vital issue in modern life. Despite extensive considerations, it remains uncertain how pollution mediates neurodegeneration in AD. Beta‐amyloids and hyperphosphorylated tau proteins are the two main pathological markers that have been studied in AD so far. Tau protein is basically a phosphoprotein whose functions are controlled by phosphorylation. The function of tau protein is to be located on the surface of microtubules and stabilize them. Studies have shown that phosphorylated tau protein (p‐tau) exists in cis and trans conformations at Thr231, among which cis is highly neurotoxic. The Pin1 enzyme performs the conversion of cis to trans or vice versa. In this study, an experimental mouse model was designed to investigate the formation of cis p‐tau by inducing air pollution. In this way, mice were randomly exposed to pollution at 2‐week, 1‐month, and 2‐month intervals. We investigated the formation of phosphorylated cis tau form during air pollution on mouse brains using Western blots and immunofluorescence. The fluorescent imaging results and Western blotting analysis of mouse brains revealed a significant accumulation of cis p‐tau in pollution‐treated mice models compared to the healthy control mice. According to Western blot results, air pollution induction caused a significant decrease in Pin1 protein. The results clearly show that the tauopathy observed during air pollution is mediated through the formation of cis tau. Our findings unravel tauopathy mysteries upon pollution and would help find a possible therapeutic target to fight the devastating disorder caused by modern life.

Tauopathy

Tau protein

10.1002/bab.2660

Cite

Citations (0)