Background: Preventing plaque rupture is the most effective strategy to reduce acute coronary syndrome (ACS). Monocytes/macrophages play a key role in plaque destabilization and rupture leading to ...
In order to perform therapy for carcinomatous peritonitis by a new angiogenesis inhibitor, TNP-470, we investigated the effective timing and the optimal doses for intraperitoneal administration using two mice models. In both carcinomatous peritonitis models caused by M 5076 tumor and B 16 melanoma, the early administration of TNP-470 within one week after tumor inoculation extended the survival times of the mice receiving the drugs, whereas the administration of TNP-470 one week or later after inoculation did not affect the survival time. However, there were significant differences in the effective therapeutic doses of TNP-470 between the two models. It is important to select the best timing and doses for intraperitoneal administration of TNP-470 based on the state of angiogenesis and the sensitivity of the tumor tissues to TNP-470.
The pathological changes brought about by the i.p. administration of a new dosage format, cisplatin microcrystals suspended in oil (CDDP-Oil), was examined in mice. CDDP-Oil decreased the absolute weight and the relative weight (organ weight/body weight) of the kidney, liver and spleen in the mice receiving the dosage form. However, the severity of the reduction of the organ weight in the CDDP-Oil administration groups was not different from that in the cisplatin aqueous solution (CDDP-Sol) administration groups. Histologically, severe degeneration and atrophy were recognized in the kidney, large intestine, small intestine, thymus, spleen, bone marrow and lymph nodes in the CDDP-Oil administration groups as well as CDDP-Sol administration groups. However, there were no additional changes in the macroscopic and microscopic findings in the CDDP-Oil groups. From these results, we concluded that this dosage form did not change the toxicity of cisplatin in terms of pathological effects.
The authors propose a new granulation technique employing a spray fluidized bed granulator that directly produces granules from liquid materials. Instant coffee dissolved in water was used as the liquid material. In order to elucidate the agglomeration mechanisms in the granulator, changes in the physical properties of granules were measured at various liquid material feed rates. We proposed an index R that is related to the liquid feed rate and is capable of quantitatively evaluating the conditions of drying in the granulator. Experimental results showed that granule formation depends on the R value in accordance with the following three mechanisms: 1) generation of fine particles by the spray drying effect, 2) agglomeration of fine particles, and 3) layering of fine particles onto the surface of individual agglomerates. Spherical granule products with high bulk density were obtained especially with layering granulation in a stable fluidized bed. Further, an equation derived from the mass balance for coffee solids in the granulator accurately described the growth of granules during layering granulation.† This report was originally printed in J. Soc. Powder Technology, Japan. 36(5), 368-377 (1999) in Japanese, before being translated into English by KONA Editorial Committee with the permission of the editorial committee of the Soc. Powder Technology, Japan.
Point mutations that activate the Ki-ras proto-oncogene are present in approximately 50% of human colorectal tumors and the activated Ki-ras gene is considered to play an important role in colorectal cancer cell proliferation. Five different colon cancer cell lines and two kinds of control cell lines were treated with antisense oligonucleotides complementary to the messenger RNA of Ki-ras. Treatment with antisense oligonucleotides at concentrations between 10 and 40 μM significantly and dose-dependently Inhibited cell growth, colony formation and Ki-ras protein production of the colon cancer cells with activated Ki-ras, but did not affect the normal cells and colon cancer cells without Ki-ras mutation. These results show that use of synthetic oligonucleotides is an effective way of producing antisense-mediated changes in the behavior of human colon cancer cells with an activated Ki-ras gene.
