Labelling with 125IUdR at radioactivity concentrations commonly employed in studied with i.v. injected tumour cells (1.0-0.1 microCi/ml) was shown to reduce considerably the in vitro reproductive viability of mastocytoma tumour cells. Velocity sedimentation cell separation studies on mastocytoma cells that had been labelled for 12 h with 0.8 microCi/ml 125IUdR yielded a population that varied markedly between fraction with respect to distribution of label and, in parallel, with respect to induced loss of reproductive viability. A similar population of mastocytoma cells that had been labelled for 36 h with 0.01 microCi/ml 125IUdR yielded fractions where distribution of label was not associated with reduced reproductive viability. Although in vivo survival (as distinct from reproductive viability) of tumour cells injected i.v. and i.p. was not significantly altered within 7 h and 30 h respectively by the commonly used concentrations of 125IUdR, it is suggested that in studies of the fate of injected tumour cells exponentially growing cells be labelled with 125IUdR for intervals well in excess of population doubling times at concentrations less than or equal to 0.025 microCi/ml.
Concentrations of human growth hormone (HGH) in pituitary tumours measured by the tibial line assay (Young, Bahn & Randall, 1965) or assessed visually from electrophoretic patterns of pituitary extracts (Lloyd & Meares, 1965) have been shown to vary from quite low levels to concentrations greater than those present in the normal anterior pituitary gland. In the present study, HGH concentration in four normal anterior pituitary glands, obtained at autopsy, and in six pituitary tumours, resected surgically, has been estimated by radioimmunoassay and by inspection of starch-gel electrophoresis patterns. Histological examination of the tumour tissue was performed on paraffin sections of formalin-fixed specimens after staining with haematoxylin and eosin, eosin and methyl blue, and PAS-orange G. Extracts of pituitary tissue were prepared by homogenization in tris-EDTA-boric acid buffer at pH 8·9. The clear supernatant obtained after centrifugation was subjected to radioimmunoassay and to starch-gel electrophoresis. Radioimmunoassay was performed by the solid-phase
A comparison of death certificates and necropsy findings in a group of premenopausal women suggests that a number of diseases are either underdiagnosed in life. Atypical cases of intracerebral haemorrhage are frequently misdiagnosed. These occur in the frontal, temporal or parietal lobes in non-hypertensive women and may be suitable for surgical treatment. Their aetiology remains obscure. Pulmonary embolus is habitually underdiagnosed in premenopausal women and myocardial infarcts appear to be overdiagnosed. The study reemphasizes that death certificates are inaccurate and that low necropsy rates render accurate statistics of diseases in the community difficult to obtain.
Sera from DBA/2 and Quackenbush mice (which are non-immune for mastocytoma and Sarcoma 180 respectively) contain a heat-labile (56 degrees for 30 min) component(s) that inhibits the in vitro growth of DBA Mastocytoma P-815 X-2 and Sarcoma 180. Adsorption of the sera with tumour cells at 4 degrees did not eliminate the factor(s), suggesting that it is not an antibody. In liquid suspension cultures inhibitory activity was observed at concentrations of mouse serum of 10--20% and in semisolid agar clonogenic cell assays at concentrations as low as 1%. The influences of the inhibitor(s) for both tumours and in both culture systems were parallel. However, there was a quantitative difference in susceptibility to other environmental factors (FCS concentration, bicarbonate concentration, and O2 tension) between the two tumours. These results parallel the in vivo findings where intravenously injected mastocytoma cells produced more tumours than did Sarcoma 180.
This study seeks to explore the correlation between cross-generational resilience and self-esteem within Caribbean families. While Caribbean families prioritize multigenerational support, they confront significant stressors that may strain familial bonds and lead to trauma and stress-related disorders. Social stigma exacerbates these challenges, potentially impacting self-esteem and perpetuating generational effects. However, the relationship between family resilience and self-esteem remains unclear. A survey-based investigation involving 179 multigenerational Caribbean families was conducted. Each family completed a self-reported questionnaire assessing family stress levels, resilience, and self-esteem. The study revealed a notable association between family resilience and self-esteem. Families exhibiting higher resilience levels also demonstrated elevated self-esteem among their members. This research contributes to understanding self-esteem dynamics within Caribbean families, emphasizing the significant link between family resilience and individual self-esteem. It highlights the potential for generational impact. The findings underscore the necessity for culturally sensitive family life education and mental health support tailored to Caribbean families. Such initiatives are crucial for fostering the overall well-being of Caribbean families navigating unique challenges in their pursuit of resilience and self-esteem.
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