Clara Conicella

Institute of Biosciences and Bioresources

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A. Errico

University of Naples Federico II

Maria Cammareri

Institute of Biosciences and Bioresources

Federica Consiglio

National Research Council

Gaetana Cremona

Institute of Biosciences and Bioresources

Riccardo Aiese Cigliano

SOM Biotech (Spain)

Pasquale Termolino

Institute of Biosciences and Bioresources

Domenico Carputo

University of Naples Federico II

Edgardo Filippone

University of Naples Federico II

L. M. Monti

Accenture (Italy)

Rosa Paparo

Institute of Biosciences and Bioresources

Cooperative Institutions

University of Naples Federico II

National Research Council

Institute of Biosciences and Bioresources

Federico II University Hospital

Institute of Genetics and Biophysics

National Academies of Sciences, Engineering, and Medicine

Istituto di Genetica Molecolare

University of Bari Aldo Moro

University of Wisconsin–Madison

University of Pavia

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Enhanced triterpene saponin biosynthesis and root nodulation in transgenic barrel medic (Medicago truncatula Gaertn.) expressing a novel β‐amyrin synthase (AsOXA1) gene

Plant Biotechnology Journal (2008)

Massimo Confalonieri Maria Cammareri Elisa Biazzi Paola Pecchia Pedro Fevereiro

Summary Triterpene saponins are a group of bioactive compounds abundant in the genus Medicago , and have been studied extensively for their biological and pharmacological properties. In this article, we evaluated the effects of the ectopic expression of AsOXA1 cDNA from Aster sedifolius on the production of triterpene saponins in barrel medic ( Medicago truncatula Gaertn.). AsOXA1 cDNA encodes β‐amyrin synthase, a key enzyme involved in triterpene saponin biosynthesis. One of the four transgenic lines expressing AsOXA1 accumulated significantly larger amounts of some triterpenic compounds in leaf and root than did control plants. In particular, the leaf exhibited significantly higher levels of bayogenin, medicagenic acid and zanhic acid. The amounts of medicagenic acid and zanhic acid, which represent the core of the M. truncatula leaf saponins, were 1.7 and 2.1 times higher, respectively, than the amounts extracted from the control line. In root, the production of bayogenin, hederagenin, soyasapogenol E and 2β‐hydroxyoleanolic acid was increased significantly. The increase in the total amounts of triterpenic compounds observed in the leaves of transgenic lines correlated with the AsOXA1 expression level. Interestingly, the plants expressing AsOXA1 showed, under different growth conditions, improved nodulation when compared with the control line. Nodulation enhancement was also accompanied by a significant change in the soyasapogenol B content. Our results indicate that the ectopic expression of AsOXA1 in barrel medic leads to a greater accumulation of triterpene saponins and enhanced root nodulation.

Medicago truncatula

Hederagenin

Sapogenin

Medicago

Glycyrrhiza uralensis

10.1111/j.1467-7652.2008.00385.x

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Citations (62)

Centro Miglioramento Genetico Ortaggi C.N.R. Portici (Napoli)

Edgardo Filippone Clara Conicella A. Errico F. Saccardo

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Multi-omics data integration provides insights into the post-harvest biology of a long shelf-life tomato landrace

Horticulture Research (2022)

Riccardo Aiese Cigliano Riccardo Aversano Antonio Di Matteo Samuela Palombieri Pasquale Termolino

Abstract In this study we investigated the transcriptome and epigenome dynamics of the tomato fruit during post-harvest in a landrace belonging to a group of tomatoes (Solanum lycopersicum L.) collectively known as “Piennolo del Vesuvio”, all characterized by a long shelf-life. Expression of protein-coding genes and microRNAs as well as DNA methylation patterns and histone modifications were analysed in distinct post-harvest phases. Multi-omics data integration contributed to the elucidation of the molecular mechanisms underlying processes leading to long shelf-life. We unveiled global changes in transcriptome and epigenome. DNA methylation increased and the repressive histone mark H3K27me3 was lost as the fruit progressed from red ripe to 150 days post-harvest. Thousands of genes were differentially expressed, about half of which were potentially epi-regulated as they were engaged in at least one epi-mark change in addition to being microRNA targets in ~5% of cases. Down-regulation of the ripening regulator MADS-RIN and of genes involved in ethylene response and cell wall degradation was consistent with the delayed fruit softening. Large-scale epigenome reprogramming that occurred in the fruit during post-harvest likely contributed to delayed fruit senescence.

Epigenome

Reprogramming

10.1093/hr/uhab042

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Citations (18)

Production of embryo rescued hybrids between the landrace “Friariello” (Capsicum annuum var. annuum) and C. baccatum var. pendulum: phenotypic and cytological characterization

Euphytica (2018)

Gaetana Cremona Marina Iovene Giovanna Festa Clara Conicella Mario Parisi

Embryo rescue

Reciprocal cross

10.1007/s10681-018-2211-6

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Citations (11)

Karyotype studies on Pisum fulvum and Pisum sativum, using a chromosome image analysis system

Genome (1991)

A. Errico Clara Conicella Gianfranco Venora

Karyotype studies were conducted on Pisum fulvum, employing traditional and image analysis methods. Meiotic data from F 1 plants obtained from crosses between Pisum sativum and P. fulvum were also obtained and correlated with the karyotype data. The karyotypes of the wild species P. fulvum were different from the standard P. sativum karyotype for relative length and arm ratio of chromosomes III, V, and VII; an additional satellite is present in chromosome V and a longer satellite in chromosome VII. These chromosome differences resulted from two independent reciprocal interchanges, T(3–5) and T(1–7), which were determined by meiotic analysis of crosses made to translocation tester stocks.Key words: translocation, computer system, Pisum, karyotype.

10.1139/g91-017

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Citations (27)

Astersedifolioside A—C, Three New Oleane‐Type Saponins with Antiproliferative Activity.

ChemInform (2004)

Gabriella Corea Maria Iorizzi Virginia Lanzotti Maria Cammareri Clara Conicella

Abstract For Abstract see ChemInform Abstract in Full Text.

Terpene

10.1002/chin.200503176

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A transcriptionally active copia-like retroelement in Citrus limon

Cellular & Molecular Biology Letters (2009)

Bruna De Felice Robert Wilson Carolina Argenziano Ioanis Kafantaris Clara Conicella

Abstract The plant nuclear genome is largely composed of mobile DNA, which can rearrange genomes and other individual gene structure and also affect gene regulation through various promoted activities: transposition, insertion, excision, chromosome breakage, and ectopic recombination. Ty1-copia-like retrotransposon is a widespread class of transposable elements in the plant kingdom, representing a large part of the total DNA content. Here, a novel retrotransposon-like sequence was isolated and identified as the Ty1-copia-like reverse transcriptase domain (named here CLCoy1), based on the homology of known elements. Fluorescence in situ hybridization, revealed that CLCoy1 was mainly located in telomeric and sub-telomeric regions along the Citrus chromosomes. CLCoy1 composes 3.6% of the genome and, interestingly, while transposons are mostly specific to a species, this element was identified in other Citrus species such as Citrus aurantium, Fortunella margarita and Citrus paradisi, but undetected in Poncirus trifoliata. We also determined that wounding, salt and cell culture stress produced transcriptional activation of this novel retroelement in Citrus limon. The novel Ty1-copia-like element CLCoy1 may have played a major role in shaping genome structure and size during Citrus species evolution.

Retrotransposon

10.2478/s11658-008-0050-5

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Citations (32)

Multi-omics data integration provides insights into the post-harvest biology of an Italian long shelf-life tomato landrace

Riccardo Aiese Cigliano Riccardo Aversano Antonio Di Matteo Samuela Palombieri Pasquale Termolino

10.17632/jnhhh4v9zm.2

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Meiotic Mutations and Crop Improvement

Plant breeding reviews (2007)

Federica Consiglio Luigi Monti Clara Conicella Domenico Carputo Luigi Frusciante

This chapter contains sections titled: Introduction Cytological Tools Molecular Tools Meiotic Genes and Mutations Applications in Breeding Conclusion Literature Cited

10.1002/9780470168028.ch6

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Highlights of meiotic genes in Arabidopsis thaliana

AFRICAN JOURNAL OF BIOTECHNOLOGY (2003)

Federica Consiglio Clara Conicella L. M. Monti Domenico Carputo

Meiosis is a fascinating and complex phenomenon and, despite its central role in sexual plant reproduction, little is known on the molecular mechanisms involved in this process. We review the progress made in recent years using Arabidopsis thalianamutants for isolating meiotic genes. In particular, emphasis is given on the description of mutants affecting either the regular commitment to meiosis, or the mechanisms of synapsis, recombination, and cytokinesis. We believe that the isolation of genes affecting some crucial meiotic events may represent the first step towards the practical use of meiotic genes in plant breeding. The introduction of deviations in the meiotic pathway into sexual crops will have important implications for the exploitation of apomixis and sexual polyploidization. Key words: Arabidopsis, meiosis, mutants, fertility.

Synapsis

Sexual reproduction

Apomixis

Meiocyte

10.5897/ajb2003.000-1102

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Citations (5)