Tarek Khaled Elmaghraby

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Preventive effect of the flavonoid, quercetin, on hepatic cancer in rats via oxidant/antioxidant activity: molecular and histological evidences

Journal of Experimental & Clinical Cancer Research (2009)

AlaaEddeen M. Seufi Safinz S Ibrahim Tarek Khaled Elmaghraby E. S. E. Hafez

The incidence of hepatocellular carcinoma is increasing in many countries. The estimated number of new cases annually is over 500,000, and the yearly incidence comprises between 2.5 and 7% of patients with liver cirrhosis. The incidence varies between different geographic areas, being higher in developing areas; males are predominantly affected, with a 2:3 male/female ratioExperiments were designed to examine the effect of N-Nitrosodiethylamine (NDEA) as cancer-inducer compound and to confirm the preventive effect of the flavonoid quercetin on hepatocellular carcinoma in rats. Briefly, thirty six male albino rats of Wistar strain were divided into 3 groups: the 1st group was administered NDEA alone (NDEA-treated), the 2nd group was treated simultaneously with NDEA and quercetin (NDEA+Q) and the 3rd group was used as control (CON). Randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) as well as p53-specifi PCR assays were employed to determine genomic difference between treated, and control animals. Histological confirmation as well as oxidant/antioxidant status of the liver tissue was done.RAPD analysis of liver samples generated 8 monomorphic bands and 22 polymorphic bands in a total of 30-banded RAPD patterns. Cluster analysis and statistical analyses of RAPD data resulted in grouping control and NDEA+Q samples in the same group with 80% similarity cut-off value. NDEA-treated samples were clustered in a separate group. Specific PCR assay for polymorphism of P53 gene revealed a uniform pattern of allele separation in both control and NDEA+Q samples. Quercetin anticancer effect was exhibited in significant decrease of oxidative stress and significant decrease of antioxidant activity. Histopathological studies showed normal liver histology of the NDEA+Q samples. Meanwhile, several cancer-induced features were clearly observable in NDEA-treated samples.This paper demonstrated that preventive effect of quercetin on hepatocarcinoma in rats by RAPD-PCR, tracing the effect on p53 gene and by histopathological evidence. Hereby, it was proved that quercetin exerted its preventive effect via decreased oxidative stress and decreased antioxidant activity.

Pro-oxidant

Anti oxidant

10.1186/1756-9966-28-80

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Citations (116)

Combretastatin A-4 disodium phosphate and low dose gamma irradiation suppress hepatocellular carcinoma by downregulating ROCK1 and VEGF gene expression

Molecular Biology Reports (2020)

Zakaria Mohamed Alghzzawy Tarek Khaled Elmaghraby Sanaa A. Hagag Mohammed H. Awwad

Combretastatin

CD31

Immunostaining

10.1007/s11033-020-05282-0

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Citations (4)

Nanogold Assay Improves Accuracy of Conventional TB Diagnostics

Lung (2019)

Hesham El-Samadony Hassan M.E. Azzazy Mohamed Awad Tageldin Mahmoud El-Sayed Ashour Ibrahim M. Deraz

Diagnostic odds ratio

Gold standard (test)

10.1007/s00408-018-00194-0

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Citations (8)

Protective effect of deoxynucleotide triphosphates on DNA damage in different mammalian cells exposed to -radiation

The Egyptian Journal of Hospital Medicine (2002)

Tarek Khaled Elmaghraby

DNA is generally considered to be the most critical cellular target when considering the lethal, carcinogenic and mutagenic effects of drugs, radiation and environmental chemicals.So the study aim to the determination the damaging effect of -radiation on DNA and the protective effect of deoxynucleotide triphosphates (dNTPs).The study includes three cell types, lymphocytes, kidney cells of African gree monkey (Vero) and hepatocellular carcinoma of human (HePG2) exposed to 1-5 Gy of -radiation and by using fluorometric analysis of DNA unwinding (FADU) method, DNA damage was measured after radiation.The cells were divided into two groups:The first received 5x10 -5 dNTPs from 0-30 minutes after radiation, while the second group was not supplemented with deoxynucleotides.Clonogenic survival for vero and HePG2 cell lines was measured.The results revealed that the increase of irradiation dose precipitates an increase of DNA strand breaks.The slope curve of initial DNA damage and mean inactivation dose (D ) differ between vero and HepG2 cell line by a factor of up 3.5 and 2, respectively.dNTPs have clear ameliorating effect on DNA damage.FADU method can play an important role in the choice of a suitable treatment (radiation or drugs) and its dosage according to measurement of DNA damages in selective malignant tissues.Moreover, using dNTPs mixture can reduce the side effect of these treatment especially after experimentally on live mammals (mice) .

radiation damage

10.21608/ejhm.2002.18746

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Assessment of human epidermal growth factor receptor 2/neu gene amplification and expression as a biomarker for radiotherapy and hormonal-treated breast cancer patients in upper Egypt

Journal of Cancer Research and Therapeutics (2018)

Marwa Khairy Essmat Mostafa A Abdelwanis Eman Zaky Mosad Tarek Khaled Elmaghraby Mohamed I. A. Othman

Breast cancer plays major public health in Egyptian women. In upper Egypt, There is an increase in the incidence of breast cancer compared to other Egyptian areas without know the reasons. In this study, we aimed to evaluate the potential of HER-2/neu status as one of the important markers to classify the women suffering from breast cancer in upper Egypt and monitoring the responsiveness to different therapies.The present study was performed on 67 female breast cancer patients in the South Egypt Cancer Institute to evaluate HER-2/neu gene amplification and expression.Tissue samples were used for immunohistological analysis of endocrine receptors, HER-2/neu, and HER-2/neu gene amplification. In addition, the blood samples were also used to determine HER-2/neu gene expression.All statistical analyses were performed using Chi-square test. The statistical difference is considered statistically significant at P < 0.05.There was a statistically significant association between HER-2/neu gene expression and the age of patients. There is decrease in the level of HER-2/neu mRNA expression in group treated with chemotherapy and group treated with chemotherapy and radiotherapy compared to each group baseline level of HER-2/neu mRNA expression before treatment. On the contrary, the group treated with chemotherapy, radiotherapy, and hormonal therapy revealed increase on the level of HER-2/neu mRNA expression when compared with their baseline for the same patients before treatment.We need further studies on the large group of upper Egypt breast cancer patients to confirm that the level of HER-2/neu mRNA expression can be used as a marker for classified them and their response to different treatment.

Hormonal Therapy

HER2/neu

10.4103/jcrt.jcrt_42_17

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Citations (4)

Hepatoprotective effect of ferulic acid and/or low doses of γ-irradiation against cisplatin-induced liver injury in rats

Human & Experimental Toxicology (2022)

Marwa A Esmat Ahmed Osman Rasha E. Hassan Sanaa A. Hagag Tarek Khaled Elmaghraby

The therapeutic efficacy of cisplatin (CIS) is limited owing to its hepatotoxic side effects. The current study aimed to investigate the protective impact of ferulic acid (FA) and low-doses of γ-irradiation (LDR) against CIS-prompted hepatotoxicity in rats. Adult male Swiss albino rats were divided into eight groups: untreated group; FA, LDR, and CIS treated groups; and combinations of one or more of the above treatments. Post-treatment analyses included measuring redox markers like SOD and CAT activity, NO free radical content, and lipid peroxidation in liver tissue. Serum aminotransferase activities were also determined. Additionally, gene transcript levels of liver NF-ҡB-P65, caspase-1, COX-2, and IL-1β were quantified. Moreover, immunohistochemistry for caspase-3 and histopathological examinations were estimated in liver tissue. Our findings revealed increased levels of oxidative stress along with a significant reduction in anti-oxidative responses and a significant increase in serum aminotransferase activities in the CIS-intoxicated group. A similar increase was also observed in COX-2 and IL-1β transcript levels and caspase-3 enzyme activity, besides a decrease in transcript levels of NF-ҡB-p65 and caspase-1, indicating an overall inflammatory trend and an increase in the apoptotic shift. The co-administration of FA and/or treatment with LDR has ameliorated the hepatotoxic effect induced by CIS. The histopathological investigation of liver tissues confirmed this ameliorating action of these adjuvant therapies against CIS toxicity. In conclusion, it is plausible to suggest that the hepatoprotective effects of co-administration of FA and/or LDR against CIS-induced hepatotoxicity are attributed to the possession of anti-oxidative, anti-inflammatory, and anti-apoptotic capabilities.

10.1177/09603271221136205

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Citations (13)

Effect of Vascular Disrupting Agent (CA-4DP) and Ionizing Radiation on Tumor Growth in Rats

Journal of Basic and Environmental Sciences (2019)

Zakaria Mohamed Alghzzawy Tarek Khaled Elmaghraby Sanaa A. Hagag M. H. Awwad

10.21608/jbes.2019.370595

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Citations (0)