Lizeth Fuentes‐Mera

Universidad Autónoma de Nuevo León

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Alberto Camacho

Universidad Autónoma de Nuevo León

César López‐Camarillo

Universidad Autónoma de la Ciudad de México

Vanessa Pérez‐Silos

University of Illinois Chicago

Nidia K. Moncada-Saucedo

Universidad Autónoma de Nuevo León

Laurence A. Marchat

Instituto Politécnico Nacional

Iván Alberto Marino-Martínez

Universidad Autónoma de Nuevo León

Jorge Lara‐Arias

Universidad Autónoma de Nuevo León

Víktor J Romero-Díaz

Universidad Autónoma de Nuevo León

Roger Maldonado‐Ruiz

Universidad Autónoma de Nuevo León

Larisa Montalvo-Martínez

Universidad Autónoma de Nuevo León

Cooperative Institutions

Universidad Autónoma de Nuevo León

Universidad Nacional Autónoma de México

Instituto Politécnico Nacional

Center for Research and Advanced Studies of the National Polytechnic Institute

Hospital Universitario Dr José Eleuterio Gonzalez

Centro de Investigación y Desarrollo

Instituto Nacional de Cancerología

Universidad Autónoma de la Ciudad de México

Hospital General Dr. Manuel Gea Gonzalez

Tecnológico de Monterrey

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Dystrophin Dp71 is required for neurite outgrowth in PC12 cells

Experimental Cell Research (2004)

Rosalinda Acosta Cecilia Montáñez Lizeth Fuentes‐Mera Everardo González-Rodríguez Pablo Gómez

Neurite

10.1016/j.yexcr.2004.01.015

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Citations (39)

Mesenchymal Stem Cells Subpopulations: Application for Orthopedic Regenerative Medicine

Stem Cells International (2016)

Vanessa Pérez‐Silos Alberto Camacho Lizeth Fuentes‐Mera

Research on mesenchymal stem cells (MSCs) continues to progress rapidly. Nevertheless, the field faces several challenges, such as inherent cell heterogeneity and the absence of unique MSCs markers. Due to MSCs’ ability to differentiate into multiple tissues, these cells represent a promising tool for new cell‐based therapies. However, for tissue engineering applications, it is critical to start with a well‐defined cell population. Additionally, evidence that MSCs subpopulations may also feature distinct characteristics and regeneration potential has arisen. In this report, we present an overview of the identification of MSCs based on the expression of several surface markers and their current tissue sources. We review the use of MSCs subpopulations in recent years and the main methodologies that have addressed their isolation, and we emphasize the most‐used surface markers for selection, isolation, and characterization. Next, we discuss the osteogenic and chondrogenic differentiation from MSCs subpopulations. We conclude that MSCs subpopulation selection is not a minor concern because each subpopulation has particular potential for promoting the differentiation into osteoblasts and chondrocytes. The accurate selection of the subpopulation advances possibilities suitable for preclinical and clinical studies and determines the safest and most efficacious regeneration process.

Regenerative Medicine

10.1155/2016/3187491

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Citations (43)

A Bioactive Cartilage Graft of IGF1-Transduced Adipose Mesenchymal Stem Cells Embedded in an Alginate/Bovine Cartilage Matrix Tridimensional Scaffold

Stem Cells International (2019)

Nidia K. Moncada-Saucedo Iván Alberto Marino-Martínez Jorge Lara‐Arias Víktor J Romero-Díaz Alberto Camacho

Articular cartilage injuries remain as a therapeutic challenge due to the limited regeneration potential of this tissue. Cartilage engineering grafts combining chondrogenic cells, scaffold materials, and microenvironmental factors are emerging as promissory alternatives. The design of an adequate scaffold resembling the physicochemical features of natural cartilage and able to support chondrogenesis in the implants is a crucial topic to solve. This study reports the development of an implant constructed with IGF1-transduced adipose-derived mesenchymal stem cells (immunophenotypes: CD105+, CD90+, CD73+, CD14-, and CD34-) embedded in a scaffold composed of a mix of alginate/milled bovine decellularized knee material which was cultivated in vitro for 28 days (3CI). Histological analyses demonstrated the distribution into isogenous groups of chondrocytes surrounded by a de novo dense extracellular matrix with balanced proportions of collagens II and I and high amounts of sulfated proteoglycans which also evidenced adequate cell proliferation and differentiation. This graft also shoved mechanical properties resembling the natural knee cartilage. A modified Bern/O'Driscoll scale showed that the 3CI implants had a significantly higher score than the 2CI implants lacking cells transduced with IGF1 (16/18 vs. 14/18), representing high-quality engineering cartilage suitable for in vivo tests. This study suggests that this graft resembles several features of typical hyaline cartilage and will be promissory for preclinical studies for cartilage regeneration.

Chondrogenesis

Decellularization

Hyaline cartilage

CD90

Matrix (chemical analysis)

10.1155/2019/9792369

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Citations (6)

Additional file 4: of Suppression of cell migration is promoted by miR-944 through targeting of SIAH1 and PTP4A1 in breast cancer cells

Figshare (2016)

Ali Flores‐Pérez Laurence A. Marchat Sergio Rodrı́guez-Cuevas Verónica Bautista Lizeth Fuentes‐Mera

Raw data obtained from gene expression studies using DNA microarrays. (XLS 2.36 mb)

10.6084/m9.figshare.c.3613406_d3

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Citations (0)

Obesogenic diet intake during pregnancy programs aberrant synaptic plasticity and addiction-like behavior to a palatable food in offspring

Behavioural Brain Research (2017)

Alberto Camacho Larisa Montalvo-Martínez Robbi E. Cardenas-Perez Lizeth Fuentes‐Mera Lourdes Garza‐Ocañas

Conditioned place preference

10.1016/j.bbr.2017.05.014

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Citations (30)

Maternal Flavonoids Intake Reverts Depression-Like Behaviour in Rat Female Offspring

Nutrients (2019)

Ana Laura de la Garza Miguel A. Garza-Cuellar Ivan A. Silva-Hernandez Robbi E. Cardenas-Perez Luis A. Reyes‐Castro

Maternal hypercaloric exposure during pregnancy and lactation is a risk factor for developing diseases associated with inflammation such as obesity, diabetes and, neurological diseases in the offspring. Neuroinflammation might modulate neuronal activation and flavonoids are dietary compounds that have been proven to exert anti-inflammatory properties. Thus, the aim of the present study is to evaluate the effect of maternal supplementation with flavonoids (kaempferol-3-O-glucoside and narirutin) on the prevention of depression-like behaviour in the female offspring of dams fed with an obesogenic diet during the perinatal period. Maternal programming was induced by high fat (HFD), high sugar (HSD), or cafeteria diets exposure and depressive like-behaviour, referred to as swimming, climbing, and immobility events, was evaluated around postnatal day 56⁻60 before and after 30 mg/kg i.p. imipramine administration in the female offspring groups. Central inflammation was analyzed by measuring the TANK binding kinase 1 (TBK1) expression. We found that the offspring of mothers exposed to HSD programming failed to show the expected antidepressant effect of imipramine. Also, imipramine injection, to the offspring of mothers exposed to cafeteria diet, displayed a pro-depressive like-behaviour phenotype. However, dietary supplementation with flavonoids reverted the depression-like behaviour in the female offspring. Finally, we found that HSD programming increases the TBK1 inflammatory protein marker in the hippocampus. Our data suggest that maternal HSD programming disrupts the antidepressant effect of imipramine whereas cafeteria diet exposure leads to depressive-like behaviour in female offspring, which is reverted by maternal flavonoid supplementation.

Cafeteria

10.3390/nu11030572

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Citations (47)

La modulación epigenética de microglia modifica la expresión de citocinas proinflamatorias

Revista de Ciencias Farmaceúticas y Biomedicina (ISSN:2448-8380) (2020)

Gabriela Cruz-Carrillo Larisa Montalvo-Martínez Lizeth Fuentes‐Mera Alberto Camacho

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Anti‑inflammatory and anti‑catabolic effect of non‑animal stabilized hyaluronic acid and mesenchymal stem cell‑conditioned medium in an osteoarthritis coculture model

Molecular Medicine Reports (2020)

Mario Simental‐Mendía Sonia Lozano‐Sep�lveda Vanessa Pérez‐Silos Lizeth Fuentes‐Mera Herminia Mart�nez‐Rodr�guez

Previous clinical studies have reported the clinical effectiveness of non‑animal stabilized hyaluronic acid (NASHA) and adipose‑derived mesenchymal stromal/stem cells (MSC) in the treatment of knee osteoarthritis (OA). Unlike MSC secreted mediators, in vitro anti‑inflammatory effects of NASHA have not been evaluated. We aimed to evaluate and compare the anti‑inflammatory effect of NASHA and MSC conditioned medium (stem cell‑conditioned medium; SC‑CM), in an explant‑based coculture model of OA. Cartilage and synovial membrane from seven patients undergoing total knee arthroplasty were used to create a coculture system. Recombinant IL‑1β was added to the cocultures to induce inflammation. Four experimental groups were generated: i) Basal; ii) IL‑1β; iii) NASHA (NASHA + IL‑1β); and iv) SC‑CM (SC‑CM + IL‑1β). Glycosaminoglycans (GAG) released in the culture medium and of nitric oxide (NO) production were quantified. Gene expression in cartilage and synovium of IL‑1β, matrix metallopeptidase 13 (MMP13), ADAM metallopeptidase with thrombospondin type 1 motif 5 (ADAMTS5) and tissue inhibitor of metalloproteinases 1 (TIMP1) was measured by reverse transcription‑quantitative PCR. Media GAG concentration was decreased in cocultures with NASHA and SC‑CM (48 h, P<0.05; 72 h, P<0.01) compared with IL‑1β. Production of NO was significantly lower only in SC‑CM after 72 h (P<0.01). In cartilage, SC‑CM inhibited the expression of IL‑1β, MMP13 and ADAMTS5, while NASHA had this effect only in MMP13 and ADAMTS5. In synovium, SC‑CM decreased the expression level of MMP13 and ADAMTS5, while NASHA only decreased ADAMTS5 expression. Both NASHA and SC‑CM increased TIMP1 expression in cartilage and synovium. Treatments with NASHA and SC‑CM were shown to be a therapeutic option that may help counteract the catabolism produced by the inflammatory state in knee OA. The anti‑inflammatory mediators produced by MSC promote a lower expression of inflammatory targets in our study model.

10.3892/mmr.2020.11004

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Citations (10)

Maternal overnutrition by hypercaloric diets programs hypothalamic mitochondrial fusion and metabolic dysfunction in rat male offspring

Nutrition & Metabolism (2018)

Robbi E. Cardenas-Perez Lizeth Fuentes‐Mera Ana Laura de la Garza Iván Torre-Villalvazo Luis A. Reyes‐Castro

Maternal overnutrition including pre-pregnancy, pregnancy and lactation promotes a lipotoxic insult leading to metabolic dysfunction in offspring. Diet-induced obesity models (DIO) show that changes in hypothalamic mitochondria fusion and fission dynamics modulate metabolic dysfunction. Using three selective diet formula including a High fat diet (HFD), Cafeteria (CAF) and High Sugar Diet (HSD), we hypothesized that maternal diets exposure program leads to selective changes in hypothalamic mitochondria fusion and fission dynamics in male offspring leading to metabolic dysfunction which is exacerbated by a second exposure after weaning. We exposed female Wistar rats to nutritional programming including Chow, HFD, CAF, or HSD for 9 weeks (pre-mating, mating, pregnancy and lactation) or to the same diets to offspring after weaning. We determined body weight, food intake and metabolic parameters in the offspring from 21 to 60 days old. Hypothalamus was dissected at 60 days old to determine mitochondria-ER interaction markers by mRNA expression and western blot and morphology by transmission electron microscopy (TEM). Mitochondrial-ER function was analyzed by confocal microscopy using hypothalamic cell line mHypoA-CLU192. Maternal programming by HFD and CAF leads to failure in glucose, leptin and insulin sensitivity and fat accumulation. Additionally, HFD and CAF programming promote mitochondrial fusion by increasing the expression of MFN2 and decreasing DRP1, respectively. Further, TEM analysis confirms that CAF exposure after programing leads to an increase in mitochondria fusion and enhanced mitochondrial-ER interaction, which partially correlates with metabolic dysfunction and fat accumulation in the HFD and CAF groups. Finally, we identified that lipotoxic palmitic acid stimulus in hypothalamic cells increases Ca2+ overload into mitochondria matrix leading to mitochondrial dysfunction. We concluded that maternal programming by HFD induces hypothalamic mitochondria fusion, metabolic dysfunction and fat accumulation in male offspring, which is exacerbated by HFD or CAF exposure after weaning, potentially due to mitochondria calcium overflux.

Overnutrition

Adult male

Clinical nutrition

10.1186/s12986-018-0279-6

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Citations (51)

Functional Roles of microRNAs in Cancer: microRNomes and oncomiRs Connection

InTech eBooks (2013)

César López‐Camarillo Laurence A. Marchat Elena Arechaga‐Ocampo Elisa Azuara-Licéaga Carlos Pérez‐Plasencia

Approaches in Biomarkers Discovery and Therapeutic Targets in Cancer 72 tumor suppressors, thus they have been collectively named as "oncomiRs" [5].Deregulation of microRNAs expression strongly alters key events leading to cancer, including differentiation, proliferation, apoptosis, migration, invasion and metastasis, and chemotherapy resistance.In consequence, the identification of deregulated microRNAs in cancer and their respective targets may provide potential diagnostic and prognostic tumor biomarkers and represent new therapeutic targets for cancer therapy.

10.5772/53981

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Citations (6)