João A. Queiroz

University of Minho

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Fani Sousa

University of Beira Interior

120

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Ângela Sousa

University of Beira Interior

Carla Cruz

University of Beira Interior

Luís A. Passarinha

University of Beira Interior

Fernanda Domingues

University of Beira Interior

Patrícia Pereira

University of Coimbra

Diana Costa

University of Beira Interior

Eugénia Gallardo

University of Beira Interior

Augusto Q. Pedro

University of Aveiro

Susana Ferreira

University of Beira Interior

Cooperative Institutions

University of Beira Interior

176

University of Lisbon

Universidade do Porto

University of Coimbra

Centre National de la Recherche Scientifique

Universidade Nova de Lisboa

Universidade de São Paulo

Rede de Química e Tecnologia

University of Aveiro

Instituto Superior Técnico

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DNA-Based Hydrogels: An Approach for Multifunctional Bioapplications

Gels horizons: from science to smart materials (2018)

Diana Costa Artur J. M. Valente João A. Queiroz

Biocompatibility

Biocompatible material

10.1007/978-981-10-6077-9_13

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Citations (1)

Modeling of existing cooling towers in ASPEN PLUS using an equilibrium stage method

Energy Conversion and Management (2012)

João A. Queiroz Vitor M.S. Rodrigues Henrique A. Matos F.G. Martins

Cooling tower

10.1016/j.enconman.2012.03.030

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Citations (43)

Formulation of chitosan–TPP–pDNA nanocapsules for gene therapy applications

Nanotechnology (2010)

Vítor M. Gaspar Fani Sousa João A. Queiroz Ilídio J. Correia

The encapsulation of DNA inside nanoparticles meant for gene delivery applications is a challenging process where several parameters need to be modulated in order to design nanocapsules with specific tailored characteristics. The purpose of this study was to investigate and improve the formulation parameters of plasmid DNA (pDNA) loaded in chitosan nanocapsules using tripolyphosphate (TPP) as polyanionic crosslinker. Nanocapsule morphology and encapsulation efficiency were analyzed as a function of chitosan degree of deacetylation and chitosan-TPP ratio. The manipulation of these parameters influenced not only the particle size but also the encapsulation and release of pDNA. Consequently the transfection efficiency of the nanoparticulated systems was also enhanced with the optimization of the particle characteristics. Overall, the differently formulated nanoparticulated systems possess singular properties that can be employed according to the desired gene delivery application.

Nanocapsules

10.1088/0957-4484/22/1/015101

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Citations (68)

Biotechnology Letters (2000)

Maria Margarida Diogo Sérgio Conti Ribeiro João A. Queiroz Gabriel A. Monteiro Pierre Perrin

Sepharose

10.1023/a:1005665003375

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Citations (39)

Arginine homopeptides for plasmid DNA purification using monolithic supports

Journal of Chromatography B (2018)

Sara Cardoso Ângela Sousa João A. Queiroz Adriano Rodrigues Azzoni Fani Sousa

10.1016/j.jchromb.2018.04.031

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Citations (7)

Plasmid DNA nanogels as photoresponsive materials for multifunctional bio-applications

Journal of Biotechnology (2014)

Diana Costa Artur J. M. Valente João A. Queiroz

Linker

DNA condensation

Nanogel

10.1016/j.jbiotec.2014.10.033

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Citations (19)

Optimization of fermentation conditions for the production of human soluble catechol-O-methyltransferase by Escherichia coli using artificial neural network

Journal of Biotechnology (2012)

Renata d’Andrada Tenório Almeida Silva Susana Ferreira Maria João Bonifácio João M.L. Dias João A. Queiroz

Central composite design

Catechol

10.1016/j.jbiotec.2012.03.025

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Citations (22)

Smoothing membrane protein structure determination by initial upstream stage improvements

Applied Microbiology and Biotechnology (2019)

Augusto Q. Pedro João A. Queiroz Luís A. Passarinha

Smoothing

10.1007/s00253-019-09873-1

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Citations (10)

Purification of supercoiled G-quadruplex pDNA for in vitro transcription

Separation and Purification Technology (2016)

Tiago Santos Patrícia Pereira Fani Sousa João A. Queiroz Carla Cruz

G-quadruplex

Transcription

10.1016/j.seppur.2016.02.036

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Citations (7)

Screening of l‐histidine‐based ligands to modify monolithic supports and selectively purify the supercoiled plasmid DNA isoform

Journal of Molecular Recognition (2015)

Lúcia F. A. Amorim Fani Sousa João A. Queiroz Carla Cruz Ângela Sousa

The growing demand of pharmaceutical-grade plasmid DNA (pDNA) suitable for biotherapeutic applications fostered the development of new purification strategies. The surface plasmon resonance technique was employed for a fast binding screening of l-histidine and its derivatives, 1-benzyl-L-histidine and 1-methyl-L-histidine, as potential ligands for the biorecognition of three plasmids with different sizes (6.05, 8.70, and 14 kbp). The binding analysis was performed with different isoforms of each plasmid (supercoiled, open circular, and linear) separately. The results revealed that the overall affinity of plasmids to l-histidine and its derivatives was high (KD > 10(-8) M), and the highest affinity was found for human papillomavirus 16 E6/E7 (K(D) = 1.1 × 10(-10) M and KD = 3.34 × 10(-10) M for open circular and linear plasmid isoforms, respectively). L-Histidine and 1-benzyl-L-histidine were immobilized on monolithic matrices. Chromatographic studies of L-histidine and 1-benzyl-L-histidine monoliths were also performed with the aforementioned samples. In general, the supercoiled isoform had strong interactions with both supports. The separation of plasmid isoforms was achieved by decreasing the ammonium sulfate concentration in the eluent, in both supports, but a lower salt concentration was required in the 1-benzyl-L-histidine monolith because of stronger interactions promoted with pDNA. The efficiency of plasmid isoforms separation remained unchanged with flow rate variations. The binding capacity for pDNA achieved with the l-histidine monolith was 29-fold higher than that obtained with conventional L-histidine agarose. Overall, the combination of either L-histidine or its derivatives with monolithic supports can be a promising strategy to purify the supercoiled isoform from different plasmids with suitable purity degree for pharmaceutical applications.

10.1002/jmr.2449

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Citations (6)