Objective:To observe the curative effect of ATP-infrared biological effect technique in treatment of patients with severe vulvovaginal candidiasis(SVVC). Methods:One hundred and fifty-three patients with SVVC were observed by prospective randomized controlled trial.Seventy-seven cases in treatment group were treated with ATP-infrared biological effect technique,once a day for two times,then the cases were treated with vaginal application of miconazole nitrate suppository,400 mg per day for six days.Seventy-six cases in control group were treated with vaginal application of miconazole nitrate suppository,400 mg per day for six days.The cases were asked to record the subjective symptoms and adverse reactions every day after the start of treatment;the cases in the two groups were reexamined at 3 and 30 days after drug withdrawal;evaluation of curative effect was carried out. Results:The onset times in treatment group and control group were(0.7±0.6) hours and(19.3±8.8) hours,respectively,there was statistically significant difference(P0.01).From the first day to the fourth day after treatment,the decrease amplitude of score of subjective symptoms in treatment group was statistically significant larger than that in control group(P0.01).On the third day after treatment,the clinical effective rates in treatment group and control group were 90.91% and 73.68%,respectively;the negative conversion rates of fungal examination in treatment group and control group were 96.10% and 85.53%,respectively;the total effective rates in treatment group and control group were 89.61% and 69.74%,respectively,there was statistically significant difference in the above-mentioned indexes between the two groups(P0.05).On the thirtieth day after treatment,the clinical effective rates in treatment group and control group were 94.80% and 68.42%,respectively;the negative conversion rates of fungal examination in treatment group and control group were 96.10% and 82.89%,respectively;the total effective rates in treatment group and control group were 92.21% and 64.47%,respectively,there was statistically significant difference in the above-mentioned indexes between the two groups(P0.01). Conclusion:ATP-infrared biological effect technique can relieve the subjective symptoms of the patients with SVVC rapidly,improve short-term curative effect and long-term curative effect,and elevate the quality of life of the patients,which is an effective method to cure SVVC.
Aim: Gastric cancer (GC) is one of the most common malignant tumors. Chrysophanol has been reported to possess antitumor effects on a variety of cancers; however, its role in GC remains unclear. This study aimed to investigate the effects of chrysophanol on the proliferation, pyroptosis, migration, and invasion of GC cells. Methods: Human GC cell lines MKN 28 and AGS cells were treated with different concentrations of chrysophanol, then cell proliferation, migration, invasion and pyroptosis were determined by CCK-8, colony-forming assay, wound healing assay, Transwell assay, and flow cytometry. Cell migration and invasion were reassessed in these transfected cells following the transfection of nod-like receptor protein-3 (NLRP3) siRNA in MKN 28 and AGS cells. To examine the downstream signaling pathway of the NLRP3 signaling pathway, NLRP3, caspase-1, gasdermin-D, interleukin (IL)-1β, and IL-18 were detected by quantitative real-time-polymerase chain reaction or western blotting. Results: Chrysophanol inhibited the proliferation of GC cells, caused pyroptosis, inhibited cell migration and invasion, and increased the expression of NLRP3 inflammasomes in GC cells. Knockdown of NLRP3 inhibited the effects of chrysophanol on proliferation, pyroptosis, migration, and invasion of GC cells. Chrysophanol plays an anticancer role by enhancing NLRP3. Conclusions: Chrysophanol exerts anti-neoplastic effects in vitro in GC cells by modulating NLRP3, thus highlighting its therapeutic potential in GC.
Tumor microenvironment (TME) and metastasis are attractive characteristics for cancer prognosis. However, the potential role of the combination of these in breast cancer (BRCA) remains elusive. This study aims to develop a novel prognostic scoring model combining characteristics of tumor-infiltrating immune cells (TIICs), a primary component of the TME, and tumor metastatic properties. Quantified TIICs and metastatic features yielded panel including 12 genes. A precise prognostic scoring system named Metastatic and Immunogenomic Risk Score (MIRS) was established via a neural network-based model. MIRS could predict prognostic and therapeutic outcomes in public datasets with decent efficiency and showed better performance when compared with other published signatures. MIRS is capable of stratifying patients into high risk-group (MIRShigh) and low risk-group (MIRSlow) according to the optimal cut-off. The MIRSlow patients exhibit significantly improved survival rate compared with MIRShigh patients (P < 0.0001), higher response to chemotherapy, but lower response to immunotherapy. Conversely, higher infiltration level of TIICs and significantly prolonged survival (P = 0.029) are observed in MIRShigh patients, indicating sensitive response in immunotherapy. Surprisingly, IVL, one of genes in MIRS scoring system, is found to be associated with the prognosis of triple-negative breast cancer (TNBC). Our analysis shows that IVL might involve cell migration signaling pathways. Notably, MIRS is a webtool (https://lva85.github.io/MIRS/) that is almost universally applicable to BRCA patients in a manner independent of platform (RNA sequencing or microarray). In total, MIRS is a promising tool for prognosis and could also predict the response to several treatment options and the molecular subtype in BRCA.Funding Information: This work was supported by The Science and Technology Development Fund, Macau SAR 462 (File no. 0020/2021/A, 001/2020/ALC, SKL-QRCM (MUST)-2020-2022), and Dr. Neher’s Biophysics Laboratory for Innovative Drug Discovery, State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, China (Grant no. FRG-21-032-SKL), Zhongnanshan Medical Foundation of Guangdong Province (Grant No. ZNSA-2021016), China. NIH 1U01DK135111 and NIH National Center for Advancing Translational Sciences UL1TR001998.Declaration of Interests: The authors declare no competing interests.
Objective To explore the long-term therapy effect of children intermittent exotropia after surgery and the impact factors of acquired foveolar stereoscopic vision. Methods A total of 216 children with intermittent exotropia aged 4-15 years were accepted the orthopia surgery. Before the surgery and 1 month or over 1 year after the surgery, position of eye, binocular vision and far and near stereoscopic vision were determined. The averaged years of following up were 3.68. Re- suits One year or longer after the surgery, 90.74% of all the children acquired the near stereoscopic vision less than 60 ＂, while 74.07% acquired the far stereoscopic vision less than 60 ＂. The far and near foveolar stereoscopic visions had no significant difference. According to the age of the pa- tients, they were divided into three groups, including less than 6 years old group, 6-9 years old group and more than 9 years old group. There was no significance in far and near foveolar stereo- scopic visions among the three groups. For a long time after operation, 150 （69.44%） patients were orthotopic, 66 （30.56%） patients were not fully orthotopic. The tested positive of near stereoscopic vi- sion less than 60 ＂ had no significance between orthotopic patients and nor fully orthotopic patients. The tested positive of the far stereoscopic vision less than 60 ＂ in orthotopic patients was 80.00%,while that in nor fully orthotopic patients was 60.61%. The difference of the test positive was signif- icant （x^2=12.17, P 〈0.01）. The logistic analysis showed that the tested positive of the far foveolar ste- reoscopic visions was associated with whether the patients were with far stereoscopic vision before surgery. Orthotopic maintain long-term after surgery was associated with the tested positive of foveo- lar stereoscopic vision. Conclusions The far and near stereoscopic vision are improved after chil- dren intermittent exotropia surgery without age influence. The impact factor of far foveolar stereo- scopic vision formation is eye position regression. The far stereoscopic vision before surgery is bene- ficial for foveolar stereoscopic vision formation after surgery. So the best opportunity for the surgery is before the loss of far stereoscopic vision.
Key words:
Intermittent exotropia; Children; Stereoscopic vision; Surgery
To investigate whether miR-125b regulates the osteogenic differentiation of bone marrow mesenchymal stem cells (MSCs) by modulating Smad4, a predicted target in silicon.Smad4 3'-UTR-luciferase vector was constructed and dual-luciferase reporter gene assay was employed to examine the effect of miR-125b on luciferase activity. MSCs were isolated and cultured from human bone marrow, and then transfected with miR-125b mimics followed by induction of osteogenic differentiation. qRT-PCR and Western blot were used to detect the expressions of Smad4 mRNA and protein. MSCs were induced into the osteoblasts after transfecting with Smad4 siRNA, and the effect of Smad4 downregulation on osteogenic differentiation was observed by AKP activity and RUNX2 mRNA levels.miR-216b bound Smad4 3'-UTR and inhibited the luciferase activity (P<0.05). Smad4 mRNA and protein expressions were significantly down-regulated in the MSCs induced into osteogenic differentiation when miR-125b was overexpressed. Downregulation of Smad4 suppressed the AKP activity and RUNX2 mRNA expression, indicating that Smad4 siRNA simulated at least in part the function of miR-125b as the regulator of MSCs osteogenic differentiation.miR-125b can suppress MSCs osteogenic differentiation by directly targeting Smad4.
Metastasis-associated processes are the predominant instigator of fatalities linked to cancer, wherein the pivotal role of circulating tumor cells lies in the resurgence of malignant growth. In recent epochs, exosomes, constituents of the extracellular vesicle cohort, have garnered attention within the field of tumor theranostics owing to their inherent attributes encompassing biocompatibility, modifiability, payload capacity, stability, and therapeutic suitability. Nonetheless, the rudimentary functionalities and limited efficacy of unmodified exosomes curtail their prospective utility. In an effort to surmount these shortcomings, intricate methodologies amalgamating nanotechnology with genetic manipulation, chemotherapy, immunotherapy, and optical intervention present themselves as enhanced avenues to surveil and intercede in tumor metastasis and relapse. This review delves into the manifold techniques currently employed to engineer exosomes, with a specific focus on elucidating the interplay between exosomes and the metastatic cascade, alongside the implementation of tailored exosomes in abating tumor metastasis and recurrence. This review not only advances comprehension of the evolving landscape within this domain but also steers the trajectory of forthcoming investigations.
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)