SARS-CoV-2, a highly contagious coronavirus, is responsible for the global pandemic of COVID-19 in 2019. Currently, it remains uncertain whether SARS-CoV-2 possesses oncogenic or oncolytic potential in influencing tumor progression. Therefore, it is important to evaluate the clinical and functional role of SARS-CoV-2 on tumor progression. Here, we integrated bioinformatic analysis of COVID-19 RNA-seq data from the GEO database and performed functional studies to explore the regulatory role of SARS-CoV-2 in solid tumor progression, including lung, colon, kidney and liver cancer. Our results demonstrate that infection with SARS-CoV-2 is associated with a decreased expression of genes associated with cancer proliferation and metastasis in lung tissues from patients diagnosed with COVID-19. Several cancer proliferation or metastasis related genes were frequently downregulated in SARS-CoV-2 infected intestinal organoids and human colon carcinoma cells. In vivo and in vitro studies revealed that SARS-CoV-2 nucleocapsid (N) protein inhibits colon and kidney tumor growth and metastasis through the N-terminal (NTD) and the C-terminal domain (CTD). The molecular mechanism indicates that the N protein of SARS-CoV-2 interacts with YBX1, resulting in the recruitment of PKM mRNA into stress granules mediated by G3BP1. This process ultimately destabilizes PKM expression and suppresses glycolysis. Our study reveals a new function of SARS-CoV-2 nucleocapsid protein on tumor progression.
Currently, there is still controversy on postoperative adjuvant chemotherapy for node-negative advanced gastric cancer. Herein, we sought to evaluate the role of postoperative adjuvant chemotherapy in these patients. We retrospectively analyzed the clinical and pathological characteristics of 363 node-negative advanced gastric cancer patients in our hospital from 1996 to 2007 who underwent gastrectomy and D2 lymphadenectomy. We compared the survival rate of the surgery-only group with that of the adjuvant chemotherapy treatment group. The 5-year survival rates of patients in the surgery-only group and the chemotherapy treatment group were 70.7% and 73.8%, respectively. There was no significant difference in the survival rate between patients receiving postoperative chemotherapy and patients not receiving chemotherapy (P=0.328). However, postoperative chemotherapy treatment significantly increased the survival rate of pT4aN0M0 patients (P=0.020), although it did not exert a direct effect on the survival rate in pT2N0M0 and pT3N0M0 patients (P=0.990 and P=0.895). We also summarized and analyzed the side effects and safety of postoperative adjuvant chemotherapy. The rate of chemotherapy-related adverse events was 79.9%. Although 61 (36.1%) patients had to adjust their chemotherapy dose, no patient died from side effects. In conclusion, postoperative chemotherapy treatment is safe but did not show a direct impact on the survival rate of the node-negative advanced gastric cancer patients. However, pT4aN0M0 patients can benefit from postoperative adjuvant chemotherapy after undergoing D2 radical resections.
【Objective】To discuss the value of hysteroscopy and dilatation and curettage (D C) in endometrial carcinoma diagnosis. 【Methods】72 endometrium carcinoma patients were invided into two groups, examined by D C (group A, 32 patients) or hysteroscopy (group B, 40 patients). The diagnosis's veracity of the two groups was compared.【Results】To estimate the involvement of cervical canal, the accuracy of group B was higher than that of group A (P 0.05). The positive rate of peritoneal cytology examination and the accuracy of estimating the no involvement of cervical canal were comparable in the two groups (P 0.05).【Conclusion】To find the involvement of cervical mucosa with endometrial carcinoma, hysteroscopy can improve the accuracy, and does not increase the positive rate of peritoneal cytology examination.
Cryptocurrency is a blockchain application that has attracted the attention by many users. The proof-of-work consensus mechanism is adopted by several cryptocurrencies, such as Bitcoin, Litecoin, and Dogecoin. This work discusses how miners choose mining pools (MPs) and how MP operators adjust pool fees to maximize their revenue. This work proposes a mining difficulty adjustment method and a randomly distributed round-batch computing power competition algorithm for mining simulation. The simulation results show that by increasing the pool fees, the large MP can increase the revenue by about 60%, and the ability to withstand the risk of changes in computing power. Small MPs can sacrifice about 35% of the revenue in exchange for the willingness of miners to stay by lowering the fees.
Gastric/gastroesophageal junction (G/GEJ) cancer represents a significant global health challenge. Radical surgery remains the cornerstone of treatment for resectable G/GEJ cancer. Supported by robust evidence from multiple clinical studies, therapeutic approaches, including adjuvant chemotherapy or chemoradiation, and perioperative chemotherapy, are generally recommended to reduce the risk of recurrence and enhance long-term survival outcomes post-surgery. In recent years, immune checkpoint inhibitors (ICIs) have altered the landscape of systemic treatment for advanced or metastatic G/GEJ cancer, becoming the standard first-line therapy for specific patients. Consequently, exploring the efficacy of ICIs in the adjuvant or neoadjuvant setting for resectable G/GEJ cancer is worthwhile. This review summarizes the current advances in the application of ICIs for resectable G/GEJ cancer.
The preparation of-cyclodextrin(-CD) inclusion compound of supercritical fluid extraction(SFECO2) of Zhizihoupo decoction(ZZHPT-SFE) by saturated water solution method was optimized by orthogonal design with the yield of inclusion compound and the inclusion ratios of honokiol and magnolol as the indexes. The optimal inclusion process was carried out at 50 ℃ for 1 h with the weight ratio of ZZHPT-SFE to-CD of 1∶9. The formation of the inclusion compound was confirmed by thin layer chromatography(TLC), scanning electron microscope(SEM) and differential scanning calorimetry(DSC), respectively.
In recent years, with the development of imaging technology, the accurate diagnosis of precancerous lesions of digestive system and early lymphoma has attracted wide attention in the medical field.In this study, 82 patients with gastrointestinal diseases, including 32 patients with early gastrointestinal lymphoma and 50 patients with gastrointestinal precancerous lesions, underwent dynamic contrast-enhanced computed tomography (CT) scanning. The difference (δ1, δ2) and ratio (Q1, Q2) of density between arterial phase, portal phase and plain scan were measured and compared, and the receiver operating characteristic (ROC) curve of the subjects was drawn.The results showed no statistically significant differences in the general condition of patients or a difference for the results of the arterial phase δ1 and Q1 between the two groups (P>0.05). However, the portal venous phase δ2 and Q2 in the early lymphoma group and in precancerous lesion group were 29.50±6.05, 41.55±10.10 Hounsfield units (HU), and 1.70±0.05, 2.06±0.31, respectively. The area under the ROC curve (AUC) values for δ2 and Q2 to identify the two diseases were 0.755 and 0.878, respectively. When δ2 and Q2 were 35.63 and 1.86 HU, the specificity was 89.60% and 67.50%, and sensitivity was 89.60% and 64.90%, respectively. When the two indexes, δ2 and Q2, were combined, the specificity and sensitivity of diagnosis were 98.99% and 56.80%, respectively.Dynamic contrast-enhanced CT can effectively distinguish early gastrointestinal lymphoma from precancerous lesions and improve the diagnostic accuracy.
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)