Lin Gan

RWTH Aachen University

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Liuyan Yang

Shanghai Academy of Agricultural Sciences

Yan Gao

Nanjing Tech University

Guanxing Huang

Chinese Academy of Geological Sciences

Bernd Denecke

RWTH Aachen University

William H. Klein

The University of Texas MD Anderson Cancer Center

Thomas Look

University Hospital of Zurich

Roland Sonntag

Universitätsklinikum Aachen

Tim Clarner

RWTH Aachen University

Eva Verjans

RWTH Aachen University

Ralf Weiskirchen

RWTH Aachen University

Cooperative Institutions

RWTH Aachen University

Universitätsklinikum Aachen

Nanjing University

Chinese Academy of Sciences

State Key Laboratory of Pollution Control and Resource Reuse

Ministry of Water Resources of the People's Republic of China

Nanjing Hydraulic Research Institute

Westfälische Hochschule

Heidelberg University

Helmholtz Centre for Infection Research

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CREB and CREM reciprocally regulate expression of ST2 in Tregs and mediate the balance between type 1 and type 2 immune responses

Authorea (Authorea) (2024)

Sudheendra Hebbar Subramanyam Judit Turyne Hriczko Saskia J. Schulz Thomas Look Tannaz Goodarzi

Background: Regulatory T cells (T regs) are gatekeepers of immune homeostasis and characterized by expression of Foxp3, which maintains T reg identity. The cAMP response element binding protein CREB is suggested to regulate T reg function and expression, however detailed analysis of CREB function in Tregs is lacking. Methods: Mice with a Foxp3-specific knockout of CREB were generated, phenotypically characterized using flow cytometry and serological testing and Tregs were subjected to whole transcriptome, TSDR methylation and ATACseq analysis. An ovalbumin induced asthma model and a T cell transfer colitis model were performed. Results: CREB deficient T regs expand in vivo but show a reduced Foxp3 expression combined with an enhanced expression of ST2, IL-5, IL-13, IL-10, and CREM, while GATA3 was not altered. Enhanced prevalence of ST2 positive Tregs was found in different organs. CREB deficient T regs were suppressive in vitro and prevented disease activity in the Th1 model of T cell mediated transfer colitis in an IL-10 dependent way while Foxp3 CRECREB fl/fl mice showed enhanced ovalbumin mediated asthma severity and spontaneous IgE production. While TSDR methylation was unchanged in CREB deficient T regs, deficiency of CREB alters chromatin accessibility at the ST2, IL13 and CREM locus. Additional genetic CREM deficiency reversed the ST2 phenotype and worsened colitis. Conclusion: Our data suggest that the balance between CREB and CREM expression is important for the regulation of ST2 expression in T regs and for the balance between type 1 and type 2 immune responses.

Adoptive Cell Transfer

10.22541/au.172489829.93821221/v1

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Neural extracellular matrix regulates visual sensory motor integration

iScience (2024)

Jacqueline Reinhard Cornelius Mueller-Buehl Susanne Wiemann Lars Roll Veronika Luft

Visual processing depends on sensitive and balanced synaptic neurotransmission. Extracellular matrix proteins in the environment of cells are key modulators in synaptogenesis and synaptic plasticity. In the present study, we provide evidence that the combined loss of the four extracellular matrix components, brevican, neurocan, tenascin-C, and tenascin-R, in quadruple knockout mice leads to severe retinal dysfunction and diminished visual motion processing

Synaptogenesis

Perineuronal net

Tenascin

Tenascin C

10.1016/j.isci.2024.108846

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Citations (2)

Pseudomonas putida inoculation promotes submerged plant Vallisneria natans growth by carbon conversion in a plant–microbe interaction

Marine and Freshwater Research (2018)

Lin Gan Hui Zhao Aili Wang Sanshan Li Jia Liu

Submerged plant growth is limited by the reduction of underwater photosynthesis attributed to low CO2 availability, as well as light limitation associated with underwater conditions. Heterotrophic bacteria and fungi play an important role in local aqueous dissolved inorganic carbon (DIC) content surrounding submerged plants. In order to investigate the effects of carbon conversion in plant–microbe interactions on plant growth, in the present study we inoculated the plant medium of Vallisneria natans with Pseudomonas putida KT2440 and measured carbon conversion in the system, as well as several indices of plant growth. The quantity of P. putida KT2440 increased twofold because of the availability of organic matter produced by V. natans. Similarly, P. putida KT2440 supplied DIC for V. natans, improving its photosynthetic rate. Moreover, the significantly higher leaf area, specific leaf area and fresh biomass of V. natans attributed to the presence of P. putida KT2440 demonstrated that the interaction between V. natans and P. putida enhanced the efficiency of nutrient and CO2 uptake by V. natans, promoting V. natans growth. Therefore, we suggest that the carbon and oxygen microcycle based on the protocooperation of V. natans and P. putida KT2440 may accelerate the transformation of carbon to increase carbon availability to promote the growth of both plant and microbe.

Pseudomonas putida

Carbon fibers

10.1071/mf17117

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Citations (5)

A positive cis-regulatory element with a bicoid target site lies within the sea urchin Spec2a enhancer

Trends in Genetics (1993)

Lin Gan William H. Klein

Nodal signaling

Blastula

Strongylocentrotus purpuratus

10.1016/0168-9525(93)90007-5

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Citations (1)

Regulation of Spec genes in aboral ectoderm cells of sea urchin {\it Strongylocentrotus purpuratus}

Lin Gan

Strongylocentrotus purpuratus

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Study on Characteristics of Biodegradation of Fenpropathrin by Mixed Microbe

Anhui nongye kexue (2006)

Lin Gan

The activated sludge taken from an aerobic tank in wastewater disposal plant of a chemical factory was acclimated to degrade fenpropathrin.The mixed culture microbes had optimum condition: pH 7.2,30 ℃ and for 72 h.The growth of mixed culture microbes and degradation rate of fenpropathrin were related to supplement of additional carbon source in medium.A high fenpropathrin removal rate of 85 % in 72 h was achieved by supplement in 1 g/L glucose.However,in batch test,the fenpropathrin removal and degradation rate were comparatively low in 72 h during the degradation of substrate where fenpropathrin was taken as the sole source of carbon,nitrogen and energy.

Degradation

Carbon source

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CXCR7 Protein Is Not Expressed on Human or Mouse Leukocytes

The Journal of Immunology (2010)

Robert Berahovich Brian A. Zabel Mark E.T. Penfold Susanna Lewén Yu Wang

Since the discovery that CXCR7 binds to CXCL12/SDF-1α, the role of CXCR7 in CXCL12-mediated biological processes has been under intensive scrutiny. However, there is no consensus in the literature on the expression of CXCR7 protein by peripheral blood cells. In this study we analyzed human and mouse leukocytes and erythrocytes for CXCR7 protein expression, using a competitive CXCL12 binding assay as well as by flow cytometry and immunohistochemistry using multiple CXCR7 Abs. CXCR7(-/-) mice were used as negative controls. Together, these methods indicate that CXCR7 protein is not expressed by human peripheral blood T cells, B cells, NK cells, or monocytes, or by mouse peripheral blood leukocytes. CXCR7 protein is, however, expressed on mouse primitive erythroid cells, which supply oxygen to the embryo during early stages of development. These studies therefore suggest that, whereas CXCR7 protein is expressed by primitive RBCs during murine embryonic development, in adult mammals CXCR7 protein is not expressed by normal peripheral blood cells.

10.4049/jimmunol.1001660

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Citations (78)

A Hypomorphic Myogenin Allele Reveals Distinct Myogenin Expression Levels Required for Viability, Skeletal Muscle Development, and Sternum Formation

Developmental Biology (1999)

Jay L. Vivian Lin Gan Eric N. Olson William H. Klein

Sternum

10.1006/dbio.1999.9305

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Allelopathy appraisal of worm metabolites in the synergistic effect between Limnodrilus hoffmeisteri and Potamogeton malaianus on algal suppression

Ecotoxicology and Environmental Safety (2019)