Studies were conducted to characterize the pharmacokinetics and excretion of hydroxysafflor yellow A (HSYA) in rats and dogs after administration by intravenous injection or infusion. Plasma, urine, feces and bile concentrations of HSYA were measured using five validated mild HPLC methods. Linear pharmacokinetics of HSYA after the intravenous administrations were found at doses ranging from 3 to 24 mg/kg in rats and from 6 to 24 mg/kg in dogs. At a dose of 3 mg/kg, HSYA in urine, feces and bile was determined. For 48 h after dosing, the amount of urinary excretion accounted for 52.6 ± 17.9 % (range: 31.1 - 78.7 %, n = 6) of the dose, and the amount of fecal amount accounted for 8.4 ± 5.3 % (range 1.7 - 16.4 %, n = 6) of the dose. Biliary excretion amount accounted for 1.4 ± 1.0 % (range 0.4 - 2.9 %; n = 6) of the dose for 24 h after dosing. Percent plasma protein binding of HSYA ranged from 48.0 to 54.6 % at 72 h. In summary, five mild HPLC methods for the determinations of HSYA in rat plasma, urine, feces, bile and dog plasma have been developed and successfully applied to preclinical pharmacokinetics and excretion of HSYA in rats and dogs. The results of excretion studies indicated that HSYA was rapidly excreted as unchanged drug in the urine. In view of previous pharmacological work, the concentration-dependent neuroprotective effect of HSYA in rats was defined.

10.1055/s-2005-916249

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In situ forming parenteral depot systems based on poly(ethylene carbonate): Effect of polymer molecular weight on model protein release

European Journal of Pharmaceutics and Biopharmaceutics (2013)

Dafeng Chu Catherine Curdy Bernd Riebesehl Moritz Beck‐Broichsitter Thomas Kissel

Bovine serum albumin

Phosphate buffered saline

10.1016/j.ejpb.2013.05.020

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