Bioactive compounds extracted from wildAcanthopanax senticosusshowed radioprotective effects throughin vitroantioxidant activities and suppression of radiation injury in mice.
Studies have underscored the pivotal role of metastasis-associated protein 3 (MTA3) as a cancer regulator, yet its potential as a drug target across cancers necessitates comprehensive evaluation. In this study, we analyzed MTA3 expression profiles to ascertain its diagnostic and prognostic value in pan-cancers, probing associations with genetic variations and immunological characteristics. Notably, liver hepatocellular carcinoma (LIHC) exhibited the most significant correlation with MTA3. By transfection of siRNA, interference of MTA3 affected HepG2 and Hepa1-6 cell viability and migration. Through drug screening and drug-likeness evaluation among marine-derived natural products, Jaspamycin was identified as a potential hepatocellular carcinoma treatment by targeting MTA3. By applying in vitro and in vivo experiment, the inhibitory effects of Jaspamycin on hepatocellular carcinoma viability, migration, and tumor progression were observed. To assess the potential of MTA3 as an anticancer drug target, MTA3 overexpression plasmid was transfected together with Jaspamycin treatment, and observed that MTA3 upregulation counteracted the inhibitory effects of Jaspamycin on hepatocarcinoma cell proliferation and migration, underscoring the efficacy of MTA3 as a drug target in hepatocellular carcinoma drug screening. This study highlights the clinical significance of MTA3 in pan-cancer, particularly in hepatocellular carcinoma. Additionally, it identifies Jaspamycin, a marine-derived compound with promising pharmacological properties, as an effective inhibitor of MTA3 activity, suggesting its potential for hepatocellular carcinoma treatment.
Recently, there has been increased interest in the production of functional probiotic food products such as cheese, yogurt and ice cream, as well as beverages and products based on meat, fruit, chocolate, etc. However, the range of such products is extremely limited, partly due to the difficulty of maintaining the viability of probiotic microorganisms. The thermal stability of the encapsulated L. plantarum SP-A3 culture was evaluated under conditions simulating the heat treatment of fortified food products. The object of the study is nearly pure culture of L. plantarum SP-A3 from the probiotic drug Lactobacterin (Mitrogen NPO). We used deep culture method on a dense nutrient medium – MRS agar at 37°C. The test culture was encapsulated in a 2 % alginate gel by B-390 apparatus (Buchi) using a 2 % calcium lactate solution as a fixative. Two types of microcapsules were obtained and studied: 200-300 µm and 400-600 µm in size. The capsules had a rounded regular shape, matte white, uniform color and were elastic. The efficiency of encapsulation with the proposed parameters was about 89%. We studied the possibility of using a model system simulating cooling of a food product after heat treatment. Both objects pass the most critical temperature ranges from 70 to 60 °C in a similar way, the use of a model system to study the survival of microorganisms when cooling a real food product after heat treatment is adequate. The study did not identify statistically significant differences between the capsule size (200-300 µm and 400-600 µm) and the survival after heat treatment (74,7±0,7% and 68,8±1,2%, respectively). Compared to unencapsulated cells (0% in three independent experiments), microencapsulated probiotics showed higher survival rates of up to 75,4%. Thus, with the use of microencapsulated probiotics, the range of probiotic foods can be expanded.
There has been increased concern regarding the potential human health risks associated with exposure to phthalates. Research indicates that food intake is the most critical exposure pathway for phthalates. This study aimed to investigate packaged beef samples for the presence of dimethyl terephthalate (DMTP), di-n-butyl phthalate (DnBP), and diisooctyl phthalate (DiOP) and to assess their translocation from the common form of food packaging procured from various Saint-Petersburg and Leningrad region shops. The packaging samples include paper and different types of plastic. Phthalates were extracted by dichloromethane and analyzed by gas chromatography coupled with mass spectrometry (GC-MS). While DnBP had the highest mean values in beef from 34.5 to 378.5 μg·kg −1 , DiOP displayed the lowest mean values from LOD to 37 μg·kg −1 . The larger contact area and the presence of distributed fat on the surface of the minced meat resulted in significantly higher phthalate translocation than beef slices. Further, DMTP was not detected in any samples. However, the examined food packages do not meet the requirements of Russian, EU and USA legislation, as DnBP migrates to meat. Calculated maximum DnBP daily intake of 0.167 μg·kg −1 ·day −1 for chilled minced beef in vacuum packaging did not exceed tolerable daily intake (TDI) level. The most alarming results are concerning the phthalates presence in beef farmed in the Leningrad region and not subjected to any plastic packaging. A full-scale study is warranted to determine the pathways and sources of phthalates migration in the food chain.
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