The delayed wound healing especially non-healing skin wound is one of the problem in clinical practice and hot research in basic medicine.The common therapies' effects were not confirm.The induced Pluripotent Stem Cell (iPSC) technology is one of de novo approaches in regeneration medicine these years.The original iPSC was reprogrammed from rat tail fibroblast.So the concept of direct cellular reprogramming was reported versus the iPSC-based reprogramming.Thus we review the fibroblast potential "stem" characteristics and its promotion to the wound healing.
Poly(1,6-hexanediol) carbonate diols (PCDL) was synthesized using 1,6-hexanediol (HDO) and diethyl carbonate(DEC) in the presence of Ti(OBu)4 as catalyst. The polymerization reaction was carried out in a two-stage process,including atmospheric pressure and vacuum. All reaction conditions were investigated based on Ti(OBu)4. The experiment results show that it exhibits the best catalytic activity with Ti(OBu)4 accounting for 0.05 weight percent of HDO. PCDL of expected number average molecular weight is prepared rapidly with raising the reaction temperature. It is available for controlling the molecular weight and polydispersity of PCDL to alter the vacuum time. The characters of PCDL were determined by Fourier transform infrared spectrum(FT-IR),1H nuclear magnetic resonance spectrum(1H-NMR),gel permeation in chromatography(GPC) and differential scanning calorimetry (DSC). It is concluded that Ti(OBu)4 is evidenced to be an effective catalyst for transesterification. The possible reaction mechanism of Ti(OBu)4 was offered.
To compare the biological behavior of mouse osteoblast-like cells (MC3T3-E1) on hydroxyapatite (HA)-coated nanotube surface of titanium and plasma-sprayed HA (HA-PS)-coated titanium surface.The HA-coated nanotube surface of titanium were fabricated by anodization coupled with alternative immersion method (AIM). MC3T3-E1 osteoblast cells cultured in vitro were seeded onto these different surfaces; their growth states were examined by a confocal laser scanning microscope; the proliferation behavior, alkaline phosphatase (ALP) activity, osteocalcin (OCN) secretion, and analysis of osteoblastic gene expressions were also compared in detail.Significant increases in ALP activity and OCN production on days 7 and 14 (P < 0.05) were observed for AIM-coated HA (HA-AIM) surfaces. However, cells cultured on HA-AIM-coated surfaces showed a delayed growth pattern. Real-time polymerase chain reaction analyses showed significantly higher relative mRNA expression levels of osteoblastic genes (runt-related protein 2, osterix, osteopontin, OCN) in cells cultured on the HA-AIM-coated nanotube surfaces as compared with cells cultured on the HA-PS and baer Ti surfaces.The current research showed that the HA-AIM-coated nanotubular Ti surfaces enhance osteoblast differentiation, which had the potential to further improve osseointegration.
Abstract Deep carbonate reservoirs present significant hurdles for reservoir stimulation due to extreme temperatures and rapid acid-rock reactions. The dense adsorption layer has the ability to postpone the interaction between rock and hydrogen ions, which is crucial for lowering the acid rock reaction rate. Based on the surfactant and film-forming agent’s synergistic impact, the retarding agent SH-1—which has the ability to generate a dense adsorption film on the surface of rock samples—was chosen for this study. After adding synergist and corrosion inhibitor, a new type of high temperature resistant adsorption type retarding acid system ’ 0.9% SH-1 + 20% HCl + 0.1% synergist + 1.5% corrosion inhibitor ’ was developed. The retarding performance of the retarding acid system at high temperature and various parameters of acid rock reaction kinetics were studied. The results show that the new adsorption-type retarded acid system can achieve good retarding effect at high temperature and ultra-high temperature, and the retarding rate is 90 % at 180 °C. In comparison to traditional hydrochloric acid, the new adsorption-type retarded acid exhibits a lower hydrogen ion mass transfer rate and a higher acid-rock reaction activation energy. These differences can effectively lower the acid-rock reaction rate. Additionally, an examination of the etching morphology reveals that the retarded acid is more easily etched along the cracks leading to the deep core. The novel adsorption-delayed acid offers excellent retarding power, high temperature resistance, and minimal damage. Consequently, it has certain reference value for the study of acidification transformation of ultra-high temperature deep carbonate reservoirs.
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